BrabA.17296.a

Integrase, catalytic domain

CENTER ID: BrabA.17296.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17296.a.A1.GE31572 Full length( BrabA.17296.a ) 1 149
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.564
RefSeq: YP_413993.1
UniProt: Q2YML5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTVRKRGGRK RAIGTRAPML IPPAANVRWL LDFVSDQLTD GRRFRVLAVV DDCTRECLPL VADTSLSGLR VARELDRIIE RRGKPKMIVS DNGSEFTSNA ILHWVDRTKM EWHYIAPGKP IQNAFIESFN GQLRDELLNE TFFSSLTHA
NT Sequence
TTGACGGTAC GCAAGCGTGG CGGGCGTAAG CGGGCGATAG GCACCCGAGC GCCGATGTTG ATCCCGCCGG CTGCCAATGT TCGCTGGTTG CTGGACTTCG TGTCAGATCA GCTCACCGAT GGCCGCAGGT TCCGGGTGCT TGCGGTCGTC GATGATTGCA CAAGAGAATG CCTGCCTCTT GTCGCCGATA CGTCCCTTTC CGGCTTGCGG GTTGCCCGTG AGTTGGACCG GATCATCGAA AGACGTGGCA AGCCGAAGAT GATCGTCAGT GACAACGGCA GTGAGTTCAC CAGTAACGCA ATCCTGCATT GGGTGGATCG GACCAAAATG GAATGGCACT ATATAGCGCC TGGCAAACCG ATCCAGAACG CCTTCATCGA AAGCTTCAAT GGACAGCTAC GAGACGAGCT CCTGAATGAA ACGTTCTTCT CGTCACTAAC CCATGCA
Details for BrabA.17296.a.A1.GE31572
HARVESTED ON: 3/1/2011
SEQUENCED ON: 3/7/2011
EXPECTED MW: 19kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLTVRKRGG RKRAIGTRAP MLIPPAANVR WLLDFVSDQL TDGRRFRVLA VVDDCTRECL PLVADTSLSG LRVARELDRI IERRGKPKMI VSDNGSEFTS NAILHWVDRT KMEWHYIAPG KPIQNAFIES FNGQLRDELL NETFFSSLTH A
Validated NT Sequence
gnnnnnnnnn nnnnnnangg agtaccatgg ctcatcacca tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tgttgacggt acgcaagcgt ggcgggcgta agcgggcgat aggcacccga gcgccgatgt tgatcccgcc ggctgccaat gttcgctggt tgctggactt cgtgtcagat cagctcaccg atggccgcag gttccgggtg cttgcggtcg tcgatgattg cacaagagaa tgcctgcctc ttgtcgccga tacgtccctt tccggcttgc gggttgcccg tgagttggac cggatcatcg aaagacgtgg caagccgaag atgatcgtca gtgacaacgg cagtgagttc accagtaacg caatcctgca ttgggtggat cggaccaaaa tggaatggca ctatatagcg cctggcaaac cgatccagaa cgccttcatc gaaagcttca atggacagct acgagacgag ctcctgaatg aaacgttctt ctcgtcacta acccatgcat aataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgcnnnnnn aaannnnnnn nnnnnaanca nntncgngtg tannnnnnnt ccncnncccc cc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLTVRKRGG RKRAIGTRAP MLIPPAANVR WLLDFVSDQL TDGRRFRVLA VVDDCTRECL PLVADTSLSG LRVARELDRI IERRGKPKMI VSDNGSEFTS NAILHWVDRT KMEWHYIAPG KPIQNAFIES FNGQLRDELL NETFFSSLTH A
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgacggta cgcaagcgtg gcgggcgtaa gcgggcgata ggcacccgag cgccgatgtt gatcccgccg gctgccaatg ttcgctggtt gctggacttc gtgtcagatc agctcaccga tggccgcagg ttccgggtgc ttgcggtcgt cgatgattgc acaagagaat gcctgcctct tgtcgccgat acgtcccttt ccggcttgcg ggttgcccgt gagttggacc ggatcatcga aagacgtggc aagccgaaga tgatcgtcag tgacaacggc agtgagttca ccagtaacgc aatcctgcat tgggtggatc ggaccaaaat ggaatggcac tatatagcgc ctggcaaacc gatccagaac gccttcatcg aaagcttcaa tggacagcta cgagacgagc tcctgaatga aacgttcttc tcgtcactaa cccatgcata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a