BrabA.17291.a

Bacterial luciferase

CENTER ID: BrabA.17291.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17291.a.A1.GE31570 Full length( BrabA.17291.a ) 1 327
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17291.a.A1.PS01100 Full length( BrabA.17291.a ) 1 327
BrabA.17291.a.A1.PS01101 Full length( BrabA.17291.a ) 1 327

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.1160
RefSeq: YP_414504.1
UniProt: Q2YPZ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIPLSVLDLS PVSEGSDAAH SLRNTLELAQ HAERLGFHRY WLAEHHNMPG ITSAATSVVI GYVANGTSTI RVGAGGVMLP NHSPLVIAEQ FGTLASLFPG RIDFGLGRAP GTDPVTAHAL RRNLDSDANA FPQDVVELLN YFKPAEPGQR VRAVPGEGLN VPVWILGSSP FGAQLAAMLG LPYAFASHFA PAEMERAIAL YRERFEPSEY MQKPYVMLGL NVIAADTDEE ANYLFTSQLQ AFINLRSGRP GKLPAPVEGY MERLDPAAQA LVRQMLSCRV VGGPETVAKG IKAFAENTGA DELMITGMIH DHKKRLRSYE IVSNSIA
NT Sequence
ATGATACCTT TATCCGTTCT TGACCTTTCG CCCGTTTCAG AAGGCAGCGA TGCGGCCCAT TCGCTTCGCA ACACGCTGGA GCTTGCGCAG CACGCTGAAC GGCTGGGCTT CCACCGCTAC TGGCTGGCGG AACATCACAA TATGCCGGGC ATCACCAGCG CGGCTACCTC GGTCGTAATC GGCTATGTGG CCAACGGAAC CTCGACCATT CGCGTGGGGG CGGGCGGTGT CATGCTGCCG AACCATTCGC CTCTGGTGAT CGCGGAACAG TTCGGGACAC TTGCTTCGTT GTTTCCGGGC CGCATCGATT TTGGCCTTGG GCGCGCACCG GGAACAGACC CAGTTACGGC CCATGCGCTT CGCCGCAATC TGGACAGTGA CGCCAATGCG TTTCCGCAGG ATGTCGTGGA ACTGCTCAAC TATTTCAAAC CGGCCGAACC CGGCCAGCGG GTGCGGGCCG TGCCGGGCGA GGGGTTGAAC GTGCCTGTCT GGATATTGGG TTCCAGCCCG TTCGGTGCGC AGCTTGCTGC CATGCTGGGC CTGCCTTACG CCTTTGCCTC GCATTTTGCT CCGGCGGAGA TGGAGCGCGC CATCGCGCTT TATCGCGAAC GCTTCGAGCC GTCCGAATAT ATGCAGAAGC CCTATGTGAT GCTGGGCCTC AATGTGATTG CAGCCGACAC GGACGAGGAG GCAAATTATC TGTTCACCTC GCAGTTACAG GCTTTCATCA ATTTGCGCAG CGGTCGCCCC GGCAAGCTGC CTGCACCTGT CGAAGGCTAT ATGGAGCGGC TTGATCCGGC AGCACAAGCC CTCGTCCGCC AGATGCTTTC CTGCCGTGTA GTTGGCGGGC CGGAGACTGT GGCGAAGGGC ATCAAGGCCT TTGCGGAAAA TACCGGCGCA GATGAGTTGA TGATTACCGG CATGATCCAC GACCACAAAA AGCGTTTGCG CTCTTACGAG ATCGTCAGCA ATTCGATTGC G
Details for BrabA.17291.a.A1.GE31570
HARVESTED ON: 3/1/2011
SEQUENCED ON: 3/7/2011
EXPECTED MW: 37kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MIPLSVLDLS PVSEGSDAAH SLRNTLELAQ HAERLGFHRY WLAEHHNMPG ITSAATSVVI GYVANGTSTI RVGAGGVMLP NHSPLVIAEQ FGTLASLFPG RIDFGLGRAP GTDPVTAHAL RRNLDSDANA FPQDVVELLN YFKPAEPGQR VRAVPGEGLN VPVWILGSSP FGAQLAAMLG LPYAFASHFA PAEMERAIAL YRERFEPSEY MQKPYVMLGL NVIAADTDEE ANYLFTSQLQ AFINLRSGRP GKLPAPVEGY MERLDPAAQA LVRQMLSCRV VGGPETVAKG IKAFAENTGA DELMITGMIH DHKKRLRSYE IVSNSI
Validated NT Sequence
cgcaatcgaa ttgctgacga tctcgtaaga gcgcaaacgc tttttgtggt cgtggatcat gccggtaatc atcaactcat ctgcgccggt attttccgca aaggccttga tgcccttcgc cacagtctcc ggcccgccaa ctacacggca ggaaagcatc tggcggacga gggcttgtgc tgccggatca agccgctcca tatagccttc gacaggtgca ggcagcttgc cggggcgacc gctgcgcaaa ttgatgaaag cctgtaactg cgaggtgaac agataatttg cctcctcgtc cgtgtcggct gcaatcacat tgaggcccag catcacatag ggcttctgca tatattcgga cggctcgaag cgttcgcgat aaagcgcgat ggcgcgctcc atctccgccg gagcaaaatg cgaggcaaag gcgtaaggca ggcccagcat ggcagcaagc tgcgcaccga acgggctgga acccaatatc cagacaggca cgttcaaccc ctcgcccggc acggcccgca cccgctggcc gggttcggcc ggtttgaaat agttgagcag ttccacgaca tcctgcggaa acgcattggc gtcactgtcc agattgcggc gaagcgcatg ggccgtaact gggtctgttc ccggtgcgcg cccaaggcca aaatcgatgc ggcccggaaa caacgaagca agtgtcccga actgttccgc gatcaccaga ggcgaatggt tcggcagcat gacaccgccc gcccccacgc gaatggtcga ggttccgttg gccacatagc cgattacgac cgaggtagcc gcgctggtga tgcccggcat attgtgatgt tccgccagcc agtagcggtg gaagcccagc cgttcagcgt gctgcgcaag ctccagcgtg ttgcgaagcg aatgggccgc atcgctgcct tctgaaacgg gcgaaaggtc aagaacggat aaaggtatca tcgaaccagg accctgggtc tgagctnccn nggtacc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIPLSVLDL SPVSEGSDAA HSLRNTLELA QHAERLGFHR YWLAEHHNMP GITSAATSVV IGYVANGTST IRVGAGGVML PNHSPLVIAE QFGTLASLFP GRIDFGLGRA PGTDPVTAHA LRRNLDSDAN AFPQDVVELL NYFKPAEPGQ RVRAVPGEGL NVPVWILGSS PFGAQLAAML GLPYAFASHF APAEMERAIA LYRERFEPSE YMQKPYVMLG LNVIAADTDE EANYLFTSQL QAFINLRSGR PGKLPAPVEG YMERLDPAAQ ALVRQMLSCR VVGGPETVAK GIKAFAENTG ADELMITGMI HDHKKRLRSY EIVSNSIA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gataccttta tccgttcttg acctttcgcc cgtttcagaa ggcagcgatg cggcccattc gcttcgcaac acgctggagc ttgcgcagca cgctgaacgg ctgggcttcc accgctactg gctggcggaa catcacaata tgccgggcat caccagcgcg gctacctcgg tcgtaatcgg ctatgtggcc aacggaacct cgaccattcg cgtgggggcg ggcggtgtca tgctgccgaa ccattcgcct ctggtgatcg cggaacagtt cgggacactt gcttcgttgt ttccgggccg catcgatttt ggccttgggc gcgcaccggg aacagaccca gttacggccc atgcgcttcg ccgcaatctg gacagtgacg ccaatgcgtt tccgcaggat gtcgtggaac tgctcaacta tttcaaaccg gccgaacccg gccagcgggt gcgggccgtg ccgggcgagg ggttgaacgt gcctgtctgg atattgggtt ccagcccgtt cggtgcgcag cttgctgcca tgctgggcct gccttacgcc tttgcctcgc attttgctcc ggcggagatg gagcgcgcca tcgcgcttta tcgcgaacgc ttcgagccgt ccgaatatat gcagaagccc tatgtgatgc tgggcctcaa tgtgattgca gccgacacgg acgaggaggc aaattatctg ttcacctcgc agttacaggc tttcatcaat ttgcgcagcg gtcgccccgg caagctgcct gcacctgtcg aaggctatat ggagcggctt gatccggcag cacaagccct cgtccgccag atgctttcct gccgtgtagt tggcgggccg gagactgtgg cgaagggcat caaggccttt gcggaaaata ccggcgcaga tgagttgatg attaccggca tgatccacga ccacaaaaag cgtttgcgct cttacgagat cgtcagcaat tcgattgcgt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BrabA.17291.a.A1.PS01100
PURIFICATION DATe: 5/27/2011
CONCENTRATION: 57.05mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MIPLSVLDLS PVSEGSDAAH SLRNTLELAQ HAERLGFHRY WLAEHHNMPG ITSAATSVVI GYVANGTSTI RVGAGGVMLP NHSPLVIAEQ FGTLASLFPG RIDFGLGRAP GTDPVTAHAL RRNLDSDANA FPQDVVELLN YFKPAEPGQR VRAVPGEGLN VPVWILGSSP FGAQLAAMLG LPYAFASHFA PAEMERAIAL YRERFEPSEY MQKPYVMLGL NVIAADTDEE ANYLFTSQLQ AFINLRSGRP GKLPAPVEGY MERLDPAAQA LVRQMLSCRV VGGPETVAKG IKAFAENTGA DELMITGMIH DHKKRLRSYE IVSNSI
Validated NT Sequence
cgcaatcgaa ttgctgacga tctcgtaaga gcgcaaacgc tttttgtggt cgtggatcat gccggtaatc atcaactcat ctgcgccggt attttccgca aaggccttga tgcccttcgc cacagtctcc ggcccgccaa ctacacggca ggaaagcatc tggcggacga gggcttgtgc tgccggatca agccgctcca tatagccttc gacaggtgca ggcagcttgc cggggcgacc gctgcgcaaa ttgatgaaag cctgtaactg cgaggtgaac agataatttg cctcctcgtc cgtgtcggct gcaatcacat tgaggcccag catcacatag ggcttctgca tatattcgga cggctcgaag cgttcgcgat aaagcgcgat ggcgcgctcc atctccgccg gagcaaaatg cgaggcaaag gcgtaaggca ggcccagcat ggcagcaagc tgcgcaccga acgggctgga acccaatatc cagacaggca cgttcaaccc ctcgcccggc acggcccgca cccgctggcc gggttcggcc ggtttgaaat agttgagcag ttccacgaca tcctgcggaa acgcattggc gtcactgtcc agattgcggc gaagcgcatg ggccgtaact gggtctgttc ccggtgcgcg cccaaggcca aaatcgatgc ggcccggaaa caacgaagca agtgtcccga actgttccgc gatcaccaga ggcgaatggt tcggcagcat gacaccgccc gcccccacgc gaatggtcga ggttccgttg gccacatagc cgattacgac cgaggtagcc gcgctggtga tgcccggcat attgtgatgt tccgccagcc agtagcggtg gaagcccagc cgttcagcgt gctgcgcaag ctccagcgtg ttgcgaagcg aatgggccgc atcgctgcct tctgaaacgg gcgaaaggtc aagaacggat aaaggtatca tcgaaccagg accctgggtc tgagctnccn nggtacc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIPLSVLDL SPVSEGSDAA HSLRNTLELA QHAERLGFHR YWLAEHHNMP GITSAATSVV IGYVANGTST IRVGAGGVML PNHSPLVIAE QFGTLASLFP GRIDFGLGRA PGTDPVTAHA LRRNLDSDAN AFPQDVVELL NYFKPAEPGQ RVRAVPGEGL NVPVWILGSS PFGAQLAAML GLPYAFASHF APAEMERAIA LYRERFEPSE YMQKPYVMLG LNVIAADTDE EANYLFTSQL QAFINLRSGR PGKLPAPVEG YMERLDPAAQ ALVRQMLSCR VVGGPETVAK GIKAFAENTG ADELMITGMI HDHKKRLRSY EIVSNSIA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATACCTTTA TCCGTTCTTG ACCTTTCGCC CGTTTCAGAA GGCAGCGATG CGGCCCATTC GCTTCGCAAC ACGCTGGAGC TTGCGCAGCA CGCTGAACGG CTGGGCTTCC ACCGCTACTG GCTGGCGGAA CATCACAATA TGCCGGGCAT CACCAGCGCG GCTACCTCGG TCGTAATCGG CTATGTGGCC AACGGAACCT CGACCATTCG CGTGGGGGCG GGCGGTGTCA TGCTGCCGAA CCATTCGCCT CTGGTGATCG CGGAACAGTT CGGGACACTT GCTTCGTTGT TTCCGGGCCG CATCGATTTT GGCCTTGGGC GCGCACCGGG AACAGACCCA GTTACGGCCC ATGCGCTTCG CCGCAATCTG GACAGTGACG CCAATGCGTT TCCGCAGGAT GTCGTGGAAC TGCTCAACTA TTTCAAACCG GCCGAACCCG GCCAGCGGGT GCGGGCCGTG CCGGGCGAGG GGTTGAACGT GCCTGTCTGG ATATTGGGTT CCAGCCCGTT CGGTGCGCAG CTTGCTGCCA TGCTGGGCCT GCCTTACGCC TTTGCCTCGC ATTTTGCTCC GGCGGAGATG GAGCGCGCCA TCGCGCTTTA TCGCGAACGC TTCGAGCCGT CCGAATATAT GCAGAAGCCC TATGTGATGC TGGGCCTCAA TGTGATTGCA GCCGACACGG ACGAGGAGGC AAATTATCTG TTCACCTCGC AGTTACAGGC TTTCATCAAT TTGCGCAGCG GTCGCCCCGG CAAGCTGCCT GCACCTGTCG AAGGCTATAT GGAGCGGCTT GATCCGGCAG CACAAGCCCT CGTCCGCCAG ATGCTTTCCT GCCGTGTAGT TGGCGGGCCG GAGACTGTGG CGAAGGGCAT CAAGGCCTTT GCGGAAAATA CCGGCGCAGA TGAGTTGATG ATTACCGGCA TGATCCACGA CCACAAAAAG CGTTTGCGCT CTTACGAGAT CGTCAGCAAT TCGATTGCGT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA
Details for BrabA.17291.a.A1.PS01101
PURIFICATION DATe: 5/27/2011
CONCENTRATION: 39.9mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MIPLSVLDLS PVSEGSDAAH SLRNTLELAQ HAERLGFHRY WLAEHHNMPG ITSAATSVVI GYVANGTSTI RVGAGGVMLP NHSPLVIAEQ FGTLASLFPG RIDFGLGRAP GTDPVTAHAL RRNLDSDANA FPQDVVELLN YFKPAEPGQR VRAVPGEGLN VPVWILGSSP FGAQLAAMLG LPYAFASHFA PAEMERAIAL YRERFEPSEY MQKPYVMLGL NVIAADTDEE ANYLFTSQLQ AFINLRSGRP GKLPAPVEGY MERLDPAAQA LVRQMLSCRV VGGPETVAKG IKAFAENTGA DELMITGMIH DHKKRLRSYE IVSNSI
Validated NT Sequence
cgcaatcgaa ttgctgacga tctcgtaaga gcgcaaacgc tttttgtggt cgtggatcat gccggtaatc atcaactcat ctgcgccggt attttccgca aaggccttga tgcccttcgc cacagtctcc ggcccgccaa ctacacggca ggaaagcatc tggcggacga gggcttgtgc tgccggatca agccgctcca tatagccttc gacaggtgca ggcagcttgc cggggcgacc gctgcgcaaa ttgatgaaag cctgtaactg cgaggtgaac agataatttg cctcctcgtc cgtgtcggct gcaatcacat tgaggcccag catcacatag ggcttctgca tatattcgga cggctcgaag cgttcgcgat aaagcgcgat ggcgcgctcc atctccgccg gagcaaaatg cgaggcaaag gcgtaaggca ggcccagcat ggcagcaagc tgcgcaccga acgggctgga acccaatatc cagacaggca cgttcaaccc ctcgcccggc acggcccgca cccgctggcc gggttcggcc ggtttgaaat agttgagcag ttccacgaca tcctgcggaa acgcattggc gtcactgtcc agattgcggc gaagcgcatg ggccgtaact gggtctgttc ccggtgcgcg cccaaggcca aaatcgatgc ggcccggaaa caacgaagca agtgtcccga actgttccgc gatcaccaga ggcgaatggt tcggcagcat gacaccgccc gcccccacgc gaatggtcga ggttccgttg gccacatagc cgattacgac cgaggtagcc gcgctggtga tgcccggcat attgtgatgt tccgccagcc agtagcggtg gaagcccagc cgttcagcgt gctgcgcaag ctccagcgtg ttgcgaagcg aatgggccgc atcgctgcct tctgaaacgg gcgaaaggtc aagaacggat aaaggtatca tcgaaccagg accctgggtc tgagctnccn nggtacc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIPLSVLDL SPVSEGSDAA HSLRNTLELA QHAERLGFHR YWLAEHHNMP GITSAATSVV IGYVANGTST IRVGAGGVML PNHSPLVIAE QFGTLASLFP GRIDFGLGRA PGTDPVTAHA LRRNLDSDAN AFPQDVVELL NYFKPAEPGQ RVRAVPGEGL NVPVWILGSS PFGAQLAAML GLPYAFASHF APAEMERAIA LYRERFEPSE YMQKPYVMLG LNVIAADTDE EANYLFTSQL QAFINLRSGR PGKLPAPVEG YMERLDPAAQ ALVRQMLSCR VVGGPETVAK GIKAFAENTG ADELMITGMI HDHKKRLRSY EIVSNSIA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATACCTTTA TCCGTTCTTG ACCTTTCGCC CGTTTCAGAA GGCAGCGATG CGGCCCATTC GCTTCGCAAC ACGCTGGAGC TTGCGCAGCA CGCTGAACGG CTGGGCTTCC ACCGCTACTG GCTGGCGGAA CATCACAATA TGCCGGGCAT CACCAGCGCG GCTACCTCGG TCGTAATCGG CTATGTGGCC AACGGAACCT CGACCATTCG CGTGGGGGCG GGCGGTGTCA TGCTGCCGAA CCATTCGCCT CTGGTGATCG CGGAACAGTT CGGGACACTT GCTTCGTTGT TTCCGGGCCG CATCGATTTT GGCCTTGGGC GCGCACCGGG AACAGACCCA GTTACGGCCC ATGCGCTTCG CCGCAATCTG GACAGTGACG CCAATGCGTT TCCGCAGGAT GTCGTGGAAC TGCTCAACTA TTTCAAACCG GCCGAACCCG GCCAGCGGGT GCGGGCCGTG CCGGGCGAGG GGTTGAACGT GCCTGTCTGG ATATTGGGTT CCAGCCCGTT CGGTGCGCAG CTTGCTGCCA TGCTGGGCCT GCCTTACGCC TTTGCCTCGC ATTTTGCTCC GGCGGAGATG GAGCGCGCCA TCGCGCTTTA TCGCGAACGC TTCGAGCCGT CCGAATATAT GCAGAAGCCC TATGTGATGC TGGGCCTCAA TGTGATTGCA GCCGACACGG ACGAGGAGGC AAATTATCTG TTCACCTCGC AGTTACAGGC TTTCATCAAT TTGCGCAGCG GTCGCCCCGG CAAGCTGCCT GCACCTGTCG AAGGCTATAT GGAGCGGCTT GATCCGGCAG CACAAGCCCT CGTCCGCCAG ATGCTTTCCT GCCGTGTAGT TGGCGGGCCG GAGACTGTGG CGAAGGGCAT CAAGGCCTTT GCGGAAAATA CCGGCGCAGA TGAGTTGATG ATTACCGGCA TGATCCACGA CCACAAAAAG CGTTTGCGCT CTTACGAGAT CGTCAGCAAT TCGATTGCGT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA