BrabA.17285.c

Solute-binding protein/glutamate receptor:Ionotropic glutamate receptor:Bacterial extracellular solute-binding protein, family 3

CENTER ID: BrabA.17285.c
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17285.c.A2.GE31569 Mature protein( BrabA.17285.c ) 44 282
BrabA.17285.c.AE2.GE43131 Mature protein( BrabA.17285.c ) 44 282
BrabA.17285.c.B2.GE43040 Mature protein( BrabA.17285.c ) 44 282
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.17285.c.A2.PS01152 Mature protein( BrabA.17285.c ) 44 282

Molecular models

METHOD RESULTS
comparative modelling Robetta_14975

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.916
RefSeq: YP_414308.1
UniProt: Q2YNH7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLNQKIRSG NQPEQMRGLE MRGFKRIAAC AVIAGFAAIG AARAEEPLKL VIGTEGAYPP FNFINPDGSL SGFDVDIARA LCDEMKAECT FITQEWDGTI PALQAGKFDA YVSSMSITEE RKKQVDFSDK YYNTPPGVAA PKDTDIKGVT KEDLAGKTIG VQVSTTHANY AEQTFTDSTI KAYPTAEEYR LDLANGRLDA VNDDSVTLSE WLKTPDGACC KMVGTYPPDD AIHGVGVGVA FKKGRPELVE KFNAAIKAIR KNGKYKEIND KYFDFDAYSA EN
NT Sequence
ATGCGGCTGA ACCAGAAAAT CAGATCAGGG AACCAGCCGG AGCAAATGAG AGGACTTGAA ATGCGCGGTT TTAAGCGTAT TGCGGCATGT GCCGTGATTG CGGGTTTTGC GGCTATTGGG GCAGCAAGGG CTGAAGAGCC CCTCAAGCTC GTCATCGGAA CCGAGGGGGC ATATCCGCCA TTTAATTTCA TCAATCCTGA TGGCTCGCTA TCGGGCTTCG ATGTGGATAT TGCTCGGGCG CTTTGCGATG AGATGAAGGC GGAGTGCACA TTCATTACGC AGGAATGGGA TGGCACCATC CCGGCATTGC AGGCAGGCAA GTTCGACGCC TATGTGTCTT CCATGTCGAT TACGGAAGAA CGCAAGAAGC AGGTGGACTT TTCGGATAAA TATTACAACA CGCCGCCCGG CGTGGCCGCG CCCAAGGATA CCGACATCAA GGGCGTGACC AAGGAAGACC TTGCAGGCAA GACCATCGGC GTCCAGGTTT CCACAACCCA CGCGAACTAT GCCGAGCAGA CCTTCACCGA CAGCACGATC AAGGCTTATC CCACGGCGGA AGAATATCGC CTTGATCTTG CCAATGGTCG TCTCGATGCG GTCAATGACG ACAGCGTGAC CTTGTCGGAA TGGCTGAAGA CGCCGGATGG CGCCTGCTGC AAGATGGTCG GCACCTACCC GCCGGACGAT GCAATCCATG GTGTCGGCGT TGGCGTTGCC TTCAAGAAGG GCCGCCCGGA ACTGGTCGAA AAGTTCAACG CTGCCATCAA GGCTATTCGC AAGAATGGCA AGTATAAGGA AATCAACGAC AAATACTTTG ATTTCGACGC TTACAGCGCT GAAAAC
Details for BrabA.17285.c.A2.GE31569
HARVESTED ON: 3/1/2011
SEQUENCED ON: 3/7/2011
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAEEPLKLV IGTEGAYPPF NFINPDGSLS GFDVDIARAL CDEMKAECTF ITQEWDGTIP ALQAGKFDAY VSSMSITEER KKQVDFSDKY YNTPPGVAAP KDTDIKGVTK EDLAGKTIGV QVSTTHANYA EQTFTDSTIK AYPTAEEYRL DLANGRLDAV NDDSVTLSEW LKTPDGACCK MVGTYPPDDA IHGVGVGVAF KKGRPELVEK FNAAIKAIRK NGKYKEINDK YFDFDAYSA
Validated NT Sequence
cagcgctgta agcgtcgaaa tcaaagtatt tgtcgttgat ttccttatac ttgccattct tgcgaatagc cttgatggca gcgttgaact tttcgaccag ttccgggcgg cccttcttga aggcaacgcc aacgccgaca ccatggattg catcgtccgg cgggtaggtg ccgaccatct tgcagcaggc gccatccggc gtcttcagcc attccgacaa ggtcacgctg tcgtcattga ccgcatcgag acgaccattg gcaagatcaa ggcgatattc ttccgccgtg ggataagcct tgatcgtgct gtcggtgaag gtctgctcgg catagttcgc gtgggttgtg gaaacctgga cgccgatggt cttgcctgca aggtcttcct tggtcacgcc cttgatgtcg gtatccttgg gcgcggccac gccgggcggc gtgttgtaat atttatccga aaagtccacc tgcttcttgc gttcttccgt aatcgacatg gaagacacat aggcgtcgaa cttgcctgcc tgcaatgccg ggatggtgcc atcccattcc tgcgtaatga atgtgcactc cgccttcatc tcatcgcaaa gcgcccgagc aatatccaca tcgaagcccg atagcgagcc atcaggattg atgaaattaa atggcggata tgccccctcg gttccgatga cgagcttgag gggctcttca gccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAEEPLKLV IGTEGAYPPF NFINPDGSLS GFDVDIARAL CDEMKAECTF ITQEWDGTIP ALQAGKFDAY VSSMSITEER KKQVDFSDKY YNTPPGVAAP KDTDIKGVTK EDLAGKTIGV QVSTTHANYA EQTFTDSTIK AYPTAEEYRL DLANGRLDAV NDDSVTLSEW LKTPDGACCK MVGTYPPDDA IHGVGVGVAF KKGRPELVEK FNAAIKAIRK NGKYKEINDK YFDFDAYSAE N
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgaagag cccctcaagc tcgtcatcgg aaccgagggg gcatatccgc catttaattt catcaatcct gatggctcgc tatcgggctt cgatgtggat attgctcggg cgctttgcga tgagatgaag gcggagtgca cattcattac gcaggaatgg gatggcacca tcccggcatt gcaggcaggc aagttcgacg cctatgtgtc ttccatgtcg attacggaag aacgcaagaa gcaggtggac ttttcggata aatattacaa cacgccgccc ggcgtggccg cgcccaagga taccgacatc aagggcgtga ccaaggaaga ccttgcaggc aagaccatcg gcgtccaggt ttccacaacc cacgcgaact atgccgagca gaccttcacc gacagcacga tcaaggctta tcccacggcg gaagaatatc gccttgatct tgccaatggt cgtctcgatg cggtcaatga cgacagcgtg accttgtcgg aatggctgaa gacgccggat ggcgcctgct gcaagatggt cggcacctac ccgccggacg atgcaatcca tggtgtcggc gttggcgttg ccttcaagaa gggccgcccg gaactggtcg aaaagttcaa cgctgccatc aaggctattc gcaagaatgg caagtataag gaaatcaacg acaaatactt tgatttcgac gcttacagcg ctgaaaacta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BrabA.17285.c.AE2.GE43131
HARVESTED ON: 5/17/2019
SEQUENCED ON: 5/25/2019
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAEEPLKLVI GTEGAYPPFN FINPDGSLSG FDVDIARALC DEMKAECTFI TQEWDGTIPA LQAGKFDAYV SSMSITEERK KQVDFSDKYY NTPGGVAAPK DTDIKGVTKE DLAGKTIGVQ VSTTHANYAE QTFTDSTIKA YPTAEEYRLD LANGRLDAVN DDSVTLSEWL KTPDGACCKM VGTYPPDDAI HGVGVGVAFK KGRPELVEKF NAAIKAIRKN GKYKEINDKY FDFDAYSAEN AGHHHHHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag cgttttcagc gctgtaagcg tcgaaatcaa agtatttgtc gttgatttcc ttatacttgc cattcttgcg aatagccttg atggcagcgt tgaacttttc gaccagttcc gggcggccct tcttgaaggc aacgccaacg ccgacaccat ggattgcatc gtccggcggg taggtgccga ccatcttgca gcaggcgcca tccggcgtct tcagccattc cgacaaggtc acgctgtcgt cattgaccgc atcgagacga ccattggcaa gatcaaggcg atattcttcc gccgtgggat aagccttgat cgtgctgtcg gtgaaggtct gctcggcata gttcgcgtgg gttgtggaaa cctggacgcc gatggtcttg cctgcaaggt cttccttggt cacgcccttg atgtcggtat ccttgggcgc ggccacgccg cccggcgtgt tgtaatattt atccgaaaag tccacctgct tcttgcgttc ttccgtaatc gacatggaag acacataggc gtcgaacttg cctgcctgca atgccgggat ggtgccatcc cattcctgcg taatgaatgt gcactccgcc ttcatctcat cgcaaagcgc ccgagcaata tccacatcga agcccgatag cgagccatca ggattgatga aattaaatgg cggatatgcc ccctcggttc cgatgacgag cttgaggggc tcttcagcca tgggagaga
Expected Protein Sequence
AEEPLKLVIG TEGAYPPFNF INPDGSLSGF DVDIARALCD EMKAECTFIT QEWDGTIPAL QAGKFDAYVS SMSITEERKK QVDFSDKYYN TPPGVAAPKD TDIKGVTKED LAGKTIGVQV STTHANYAEQ TFTDSTIKAY PTAEEYRLDL ANGRLDAVND DSVTLSEWLK TPDGACCKMV GTYPPDDAIH GVGVGVAFKK GRPELVEKFN AAIKAIRKNG KYKEINDKYF DFDAYSAENG HHHHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccgc tgaagagccc ctcaagctcg tcatcggaac cgagggggca tatccgccat ttaatttcat caatcctgat ggctcgctat cgggcttcga tgtggatatt gctcgggcgc tttgcgatga gatgaaggcg gagtgcacat tcattacgca ggaatgggat ggcaccatcc cggcattgca ggcaggcaag ttcgacgcct atgtgtcttc catgtcgatt acggaagaac gcaagaagca ggtggacttt tcggataaat attacaacac gccgcccggc gtggccgcgc ccaaggatac cgacatcaag ggcgtgacca aggaagacct tgcaggcaag accatcggcg tccaggtttc cacaacccac gcgaactatg ccgagcagac cttcaccgac agcacgatca aggcttatcc cacggcggaa gaatatcgcc ttgatcttgc caatggtcgt ctcgatgcgg tcaatgacga cagcgtgacc ttgtcggaat ggctgaagac gccggatggc gcctgctgca agatggtcgg cacctacccg ccggacgatg caatccatgg tgtcggcgtt ggcgttgcct tcaagaaggg ccgcccggaa ctggtcgaaa agttcaacgc tgccatcaag gctattcgca agaatggcaa gtataaggaa atcaacgaca aatactttga tttcgacgct tacagcgctg aaaacgggca ccaccatcat catcattaac ggatccgaat tcgagctccg tcgacaagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gat
Details for BrabA.17285.c.B2.GE43040
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/23/2019
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA EEPLKLVIGT EGAYPPFNFI NPDGSLSGFD VDIARALCDE MKAECTFITQ EWDGTIPALQ AGKFDAYVSS MSITEERKKQ VDFSDKYYNT PPGVAAPKDT DIKGVTKEDL AGKTIGVQVS TTHANYAEQT FTDSTIKAYP TAEEYRLDLA NGRLDAVNDD SVTLSEWLKT PDGACCKMVG TYPPDDAIHG VGVGVAFKKG RPELVEKFNA AIKAIRKNGK YKEINDKYFD FDAYSAEN
Validated NT Sequence
atcttactca cttagttttc agcgctgtaa gcgtcgaaat caaagtattt gtcgttgatt tccttatact tgccattctt gcgaatagcc ttgatggcag cgttgaactt ttcgaccagt tccgggcggc ccttcttgaa ggcaacgcca acgccgacac catggattgc atcgtccggc gggtaggtgc cgaccatctt gcagcaggcg ccatccggcg tcttcagcca ttccgacaag gtcacgctgt cgtcattgac cgcatcgaga cgaccattgg caagatcaag gcgatattct tccgccgtgg gataagcctt gatcgtgctg tcggtgaagg tctgctcggc atagttcgcg tgggttgtgg aaacctggac gccgatggtc ttgcctgcaa ggtcttcctt ggtcacgccc ttgatgtcgg tatccttggg cgcggccacg ccgggcggcg tgttgtaata tttatccgaa aagtccacct gcttcttgcg ttcttccgta atcgacatgg aagacacata ggcgtcgaac ttgcctgcct gcaatgccgg gatggtgcca tcccattcct gcgtaatgaa tgtgcactcc gccttcatct catcgcaaag cgcccgagca atatccacat cgaagcccga tagcgagcca tcaggattga tgaaattaaa tggcggatat gccccctcgg ttccgatgac gagcttgagg ggctcttcag ccatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHAE EPLKLVIGTE GAYPPFNFIN PDGSLSGFDV DIARALCDEM KAECTFITQE WDGTIPALQA GKFDAYVSSM SITEERKKQV DFSDKYYNTP PGVAAPKDTD IKGVTKEDLA GKTIGVQVST THANYAEQTF TDSTIKAYPT AEEYRLDLAN GRLDAVNDDS VTLSEWLKTP DGACCKMVGT YPPDDAIHGV GVGVAFKKGR PELVEKFNAA IKAIRKNGKY KEINDKYFDF DAYSAEN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgctgaag agcccctcaa gctcgtcatc ggaaccgagg gggcatatcc gccatttaat ttcatcaatc ctgatggctc gctatcgggc ttcgatgtgg atattgctcg ggcgctttgc gatgagatga aggcggagtg cacattcatt acgcaggaat gggatggcac catcccggca ttgcaggcag gcaagttcga cgcctatgtg tcttccatgt cgattacgga agaacgcaag aagcaggtgg acttttcgga taaatattac aacacgccgc ccggcgtggc cgcgcccaag gataccgaca tcaagggcgt gaccaaggaa gaccttgcag gcaagaccat cggcgtccag gtttccacaa cccacgcgaa ctatgccgag cagaccttca ccgacagcac gatcaaggct tatcccacgg cggaagaata tcgccttgat cttgccaatg gtcgtctcga tgcggtcaat gacgacagcg tgaccttgtc ggaatggctg aagacgccgg atggcgcctg ctgcaagatg gtcggcacct acccgccgga cgatgcaatc catggtgtcg gcgttggcgt tgccttcaag aagggccgcc cggaactggt cgaaaagttc aacgctgcca tcaaggctat tcgcaagaat ggcaagtata aggaaatcaa cgacaaatac tttgatttcg acgcttacag cgctgaaaac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BrabA.17285.c.A2.PS01152
PURIFICATION DATe: 7/13/2011
CONCENTRATION: 70.58mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAEEPLKLV IGTEGAYPPF NFINPDGSLS GFDVDIARAL CDEMKAECTF ITQEWDGTIP ALQAGKFDAY VSSMSITEER KKQVDFSDKY YNTPPGVAAP KDTDIKGVTK EDLAGKTIGV QVSTTHANYA EQTFTDSTIK AYPTAEEYRL DLANGRLDAV NDDSVTLSEW LKTPDGACCK MVGTYPPDDA IHGVGVGVAF KKGRPELVEK FNAAIKAIRK NGKYKEINDK YFDFDAYSA
Validated NT Sequence
cagcgctgta agcgtcgaaa tcaaagtatt tgtcgttgat ttccttatac ttgccattct tgcgaatagc cttgatggca gcgttgaact tttcgaccag ttccgggcgg cccttcttga aggcaacgcc aacgccgaca ccatggattg catcgtccgg cgggtaggtg ccgaccatct tgcagcaggc gccatccggc gtcttcagcc attccgacaa ggtcacgctg tcgtcattga ccgcatcgag acgaccattg gcaagatcaa ggcgatattc ttccgccgtg ggataagcct tgatcgtgct gtcggtgaag gtctgctcgg catagttcgc gtgggttgtg gaaacctgga cgccgatggt cttgcctgca aggtcttcct tggtcacgcc cttgatgtcg gtatccttgg gcgcggccac gccgggcggc gtgttgtaat atttatccga aaagtccacc tgcttcttgc gttcttccgt aatcgacatg gaagacacat aggcgtcgaa cttgcctgcc tgcaatgccg ggatggtgcc atcccattcc tgcgtaatga atgtgcactc cgccttcatc tcatcgcaaa gcgcccgagc aatatccaca tcgaagcccg atagcgagcc atcaggattg atgaaattaa atggcggata tgccccctcg gttccgatga cgagcttgag gggctcttca gccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAEEPLKLV IGTEGAYPPF NFINPDGSLS GFDVDIARAL CDEMKAECTF ITQEWDGTIP ALQAGKFDAY VSSMSITEER KKQVDFSDKY YNTPPGVAAP KDTDIKGVTK EDLAGKTIGV QVSTTHANYA EQTFTDSTIK AYPTAEEYRL DLANGRLDAV NDDSVTLSEW LKTPDGACCK MVGTYPPDDA IHGVGVGVAF KKGRPELVEK FNAAIKAIRK NGKYKEINDK YFDFDAYSAE N
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTGAAGAG CCCCTCAAGC TCGTCATCGG AACCGAGGGG GCATATCCGC CATTTAATTT CATCAATCCT GATGGCTCGC TATCGGGCTT CGATGTGGAT ATTGCTCGGG CGCTTTGCGA TGAGATGAAG GCGGAGTGCA CATTCATTAC GCAGGAATGG GATGGCACCA TCCCGGCATT GCAGGCAGGC AAGTTCGACG CCTATGTGTC TTCCATGTCG ATTACGGAAG AACGCAAGAA GCAGGTGGAC TTTTCGGATA AATATTACAA CACGCCGCCC GGCGTGGCCG CGCCCAAGGA TACCGACATC AAGGGCGTGA CCAAGGAAGA CCTTGCAGGC AAGACCATCG GCGTCCAGGT TTCCACAACC CACGCGAACT ATGCCGAGCA GACCTTCACC GACAGCACGA TCAAGGCTTA TCCCACGGCG GAAGAATATC GCCTTGATCT TGCCAATGGT CGTCTCGATG CGGTCAATGA CGACAGCGTG ACCTTGTCGG AATGGCTGAA GACGCCGGAT GGCGCCTGCT GCAAGATGGT CGGCACCTAC CCGCCGGACG ATGCAATCCA TGGTGTCGGC GTTGGCGTTG CCTTCAAGAA GGGCCGCCCG GAACTGGTCG AAAAGTTCAA CGCTGCCATC AAGGCTATTC GCAAGAATGG CAAGTATAAG GAAATCAACG ACAAATACTT TGATTTCGAC GCTTACAGCG CTGAAAACTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A