SSGCID: Target Page

BrabA.13473.a

hypothetical protein

CENTER ID:	BrabA.13473.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.13473.a.A1.GE27792	Full length( BrabA.13473.a )		1	277

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.949
RefSeq:	YP_414337.1
UniProt:	Q2YNL8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNQNETHCLA LLREADRDRY LSVLYAPQEK RGALAALYAF NAEIARIRDL VHEPLPGKVR LQWWRDLING EARGSAEAHP VAAALIETIE KYELPRIAFD NYCEARVFDL YDDPMPSRND LEGYCGETAS ALIQLAGFIL DRDAAQAHAE TAGHAGVAQA VAGLLRLLPI HRRRGQVYVP ADMLKAIGVT RDMLLSSEDK SATERAVTIM LALAREHLAI FDKAKAGLPQ TLHPAFLPMA LVPAYLNAVE RLGAQAAQKV ADISAIRKQW LMFRANF

NT Sequence

ATGAACCAGA ATGAAACCCA TTGTCTGGCG CTTTTGCGCG AAGCAGACCG GGATCGCTAT CTTTCGGTGC TCTACGCCCC GCAGGAAAAG CGCGGCGCAT TGGCTGCACT ATATGCGTTC AACGCCGAGA TTGCGCGCAT CCGCGATCTC GTCCACGAAC CGCTTCCGGG CAAAGTGCGG TTGCAATGGT GGCGTGATCT CATCAATGGC GAGGCAAGAG GCAGTGCTGA AGCGCATCCA GTTGCCGCGG CCTTGATCGA GACGATCGAA AAATACGAGC TTCCCCGCAT TGCCTTCGAC AATTATTGCG AAGCGCGCGT GTTCGATCTT TATGACGATC CCATGCCGAG CCGTAACGAC TTGGAAGGCT ATTGCGGTGA AACGGCATCG GCTTTGATCC AGCTGGCCGG TTTCATACTG GATCGCGATG CCGCGCAGGC CCATGCTGAA ACCGCTGGCC ATGCCGGCGT TGCGCAGGCG GTGGCGGGTT TACTGCGGCT CTTGCCAATC CACCGGCGAC GCGGGCAGGT TTATGTTCCG GCGGACATGC TCAAAGCCAT CGGTGTAACC CGCGATATGC TTCTCTCCAG CGAAGACAAA TCCGCCACCG AGCGTGCCGT CACCATCATG CTGGCGCTGG CGCGGGAGCA TCTGGCAATT TTCGACAAGG CCAAAGCCGG GTTGCCTCAA ACGCTTCATC CGGCCTTTTT GCCGATGGCG CTTGTTCCGG CCTATCTGAA CGCGGTTGAA CGTCTGGGTG CGCAGGCAGC GCAGAAGGTT GCCGATATTT CTGCCATCCG TAAACAATGG CTGATGTTCC GCGCAAATTT T

Details for BrabA.13473.a.A1.GE27792

HARVESTED ON:	11/16/2009
SEQUENCED ON:	11/23/2009
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNQNETHCL ALLREADRDR YLSVLYAPQE KRGALAALYA FNAEIARIRD LVHEPLPGKV RLQWWRDLIN GEARGSAEAH PVAAALIETI EKYELPRIAF DNYCEARVFD LYDDPMPSRN DLEGYCGETA SALIQLAGFI LDRDAAQAHA ETAGHAGVAQ AVAGLLRLLP IHRRRGQVYV PADMLKAIGV TRDMLLSSED KSATERAVTI MLALAREHLA IFDKAKAGLP QTLHPAFLPM ALVPAYLNAV ERLGAQAAQK VADISAIRKQ WLMFRA

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaacc agaatgaaac ccattgtctg gcgcttttgc gcgaagcaga ccgggatcgc tatctttcgg tgctctacgc cccgcaggaa aagcgcggcg cattggctgc actatatgcg ttcaacgccg agattgcgcg catccgcgat ctcgtccacg aaccgcttcc gggcaaagtg cggttgcaat ggtggcgtga tctcatcaat ggcgaggcaa gaggcagtgc tgaagcgcat ccagttgccg cggccttgat cgagacgatc gaaaaatacg agcttccccg cattgccttc gacaattatt gcgaagcgcg cgtgttcgat ctttatgacg atcccatgcc gagccgtaac gacttggaag gctattgcgg tgaaacggca tcggctttga tccagctggc cggtttcata ctggatcgcg atgccgcgca ggcccatgct gaaaccgctg gccatgccgg cgttgcgcag gcggtggcgg gtttactgcg gctcttgcca atccaccggc gacgcgggca ggtttatgtt ccggcggaca tgctcaaagc catcggtgta acccgcgata tgcttctctc cagcgaagac aaatccgcca ccgagcgtgc cgtcaccatc atgctggcgc tggcgcggga gcatctggca attttcgaca aggccaaagc cgggttgcct caaacgcttc atccggcctt tttgccgatg gcgcttgttc cggcctatct gaacgcggtt gaacgtctgg gtgcgcaggc agcgcagaag gttgccgata tttctgccat ccgtaaacaa tggctgatgt tccgcgcaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaccagaat gaaacccatt gtctggcgct tttgcgcgaa gcagaccggg atcgctatct ttcggtgctc tacgccccgc aggaaaagcg cggcgcattg gctgcactat atgcgttcaa cgccgagatt gcgcgcatcc gcgatctcgt ccacgaaccg cttccgggca aagtgcggtt gcaatggtgg cgtgatctca tcaatggcga ggcaagaggc agtgctgaag cgcatccagt tgccgcggcc ttgatcgaga cgatcgaaaa atacgagctt ccccgcattg ccttcgacaa ttattgcgaa gcgcgcgtgt tcgatcttta tgacgatccc atgccgagcc gtaacgactt ggaaggctat tgcggtgaaa cggcatcggc tttgatccag ctggccggtt tcatactgga tcgcgatgcc gcgcaggccc atgctgaaac cgctggccat gccggcgttg cgcaggcggt ggcgggttta ctgcggctct tgccaatcca ccggcgacgc gggcaggttt atgttccggc ggacatgctc aaagccatcg gtgtaacccg cgatatgctt ctctccagcg aagacaaatc cgccaccgag cgtgccgtca ccatcatgct ggcgctggcg cgggagcatc tggcaatttt cgacaaggcc aaagccgggt tgcctcaaac gcttcatccg gcctttttgc cgatggcgct tgttccggcc tatctgaacg cggttgaacg tctgggtgcg caggcagcgc agaaggttgc cgatatttct gccatccgta aacaatggct gatgttccgc gcaaatttta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a