BrabA.12891.a

septum formation inhibitor

CENTER ID: BrabA.12891.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.12891.a.A1.GE27791 Full length( BrabA.12891.a ) 1 253
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.12891.a.A1.PW27679 Full length( BrabA.12891.a ) 1 253
BrabA.12891.a.A1.PW28562 Full length( BrabA.12891.a ) 1 253

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.3175
RefSeq: YP_419036.1
UniProt: Q2YJZ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPLTKMNQVL TETRPIRLKG RSFLAMVLSP ELPLDGWLER LDDLARRSSG FFLGRPVVLD MENLAIERAQ LVYLLQALND RGVWIMGVEG ARPSLLGPGM PPAMRGGQPA ADFEAPAGEP QANPGAPEPQ ISQAVRAPGH AVHAMPSMVI TEPVRSGQSV YFPEGDVTIV GSVASGAEVV AGGSIHIYGT LRGRALAGTA GNTSARIFCR KLEAELVAID GLYKTAEDLE PRFRGQAVQL WLDGDYMMID TLS
NT Sequence
TTGCCGTTAA CCAAGATGAA TCAAGTGCTA ACAGAAACTC GCCCGATCCG CCTTAAAGGG CGGTCCTTTC TTGCAATGGT GCTCTCGCCG GAACTTCCGC TCGACGGCTG GCTGGAGCGC CTGGACGATC TCGCCCGGCG TTCCTCCGGT TTTTTCCTCG GGCGCCCGGT TGTGCTCGAT ATGGAAAACC TTGCCATCGA ACGTGCGCAG CTCGTCTATC TGTTGCAGGC GTTGAACGAT CGCGGCGTCT GGATCATGGG CGTGGAAGGC GCGCGGCCTT CGCTTCTCGG GCCGGGAATG CCGCCTGCCA TGCGCGGCGG CCAGCCCGCA GCCGATTTTG AAGCACCCGC TGGTGAGCCG CAGGCAAATC CTGGCGCGCC GGAACCGCAG ATTTCGCAAG CCGTGCGTGC GCCAGGCCAT GCCGTGCACG CCATGCCTTC GATGGTTATC ACCGAGCCGG TCCGTTCCGG CCAGTCGGTC TATTTCCCGG AAGGGGATGT CACAATTGTC GGTTCGGTTG CTTCGGGTGC GGAAGTGGTC GCAGGCGGTT CGATCCATAT TTACGGCACG CTGCGCGGGC GGGCGCTGGC GGGGACGGCG GGGAATACGA GTGCGCGAAT TTTCTGTCGC AAGCTCGAAG CGGAATTGGT TGCCATCGAT GGTCTCTACA AGACCGCCGA GGATCTGGAG CCGAGGTTCC GCGGACAGGC CGTCCAGCTC TGGCTCGACG GCGATTACAT GATGATTGAT ACATTGAGC
Details for BrabA.12891.a.A1.GE27791
HARVESTED ON: 11/16/2009
SEQUENCED ON: 11/23/2009
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLPLTKMNQ VLTETRPIRL KGRSFLAMVL SPELPLDGWL ERLDDLARRS SGFFLGRPVV LDMENLAIER AQLVYLLQAL NDRGVWIMGV EGARPSLLGP GMPPAMRGGQ PAADFEAPAG EPQANPGAPE PQISQAVRAP GHAVHAMPSM VITEPVRSGQ SVYFPEGDVT IVGSVASGAE VVAGGSIHIY GTLRGRALAG TAGNTSARIF CRKLEAELVA IDGLYKTAED LEPRFRGQAV QLWLDGDYMM IDTLS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgc cgttaaccaa gatgaatcaa gtgctaacag aaactcgccc gatccgcctt aaagggcggt cctttcttgc aatggtgctc tcgccggaac ttccgctcga cggctggctg gagcgcctgg acgatctcgc ccggcgttcc tccggttttt tcctcgggcg cccggttgtg ctcgatatgg aaaaccttgc catcgaacgt gcgcagctcg tctatctgtt gcaggcgttg aacgatcgcg gcgtctggat catgggcgtg gaaggcgcgc ggccttcgct tctcgggccg ggaatgccgc ctgccatgcg cggcggccag cccgcagccg attttgaagc acccgctggt gagccgcagg caaatcctgg cgcgccggaa ccgcagattt cgcaagccgt gcgtgcgcca ggccatgccg tgcacgccat gccttcgatg gttatcaccg agccggtccg ttccggccag tcggtctatt tcccggaagg ggatgtcaca attgtcggtt cggttgcttc gggtgcggaa gtggtcgcag gcggttcgat ccatatttac ggcacgctgc gcgggcgggc gctggcgggg acggcgggga atacgagtgc gcgaattttc tgtcgcaagc tcgaagcgga attggttgcc atcgatggtc tctacaagac cgccgaggat ctggagccga ggttccgcgg acaggccgtc cagctctggc tcgacggcga ttacatgatg attgatacat tgagctaata a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLPLTKMNQ VLTETRPIRL KGRSFLAMVL SPELPLDGWL ERLDDLARRS SGFFLGRPVV LDMENLAIER AQLVYLLQAL NDRGVWIMGV EGARPSLLGP GMPPAMRGGQ PAADFEAPAG EPQANPGAPE PQISQAVRAP GHAVHAMPSM VITEPVRSGQ SVYFPEGDVT IVGSVASGAE VVAGGSIHIY GTLRGRALAG TAGNTSARIF CRKLEAELVA IDGLYKTAED LEPRFRGQAV QLWLDGDYMM IDTLS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgccgtta accaagatga atcaagtgct aacagaaact cgcccgatcc gccttaaagg gcggtccttt cttgcaatgg tgctctcgcc ggaacttccg ctcgacggct ggctggagcg cctggacgat ctcgcccggc gttcctccgg ttttttcctc gggcgcccgg ttgtgctcga tatggaaaac cttgccatcg aacgtgcgca gctcgtctat ctgttgcagg cgttgaacga tcgcggcgtc tggatcatgg gcgtggaagg cgcgcggcct tcgcttctcg ggccgggaat gccgcctgcc atgcgcggcg gccagcccgc agccgatttt gaagcacccg ctggtgagcc gcaggcaaat cctggcgcgc cggaaccgca gatttcgcaa gccgtgcgtg cgccaggcca tgccgtgcac gccatgcctt cgatggttat caccgagccg gtccgttccg gccagtcggt ctatttcccg gaaggggatg tcacaattgt cggttcggtt gcttcgggtg cggaagtggt cgcaggcggt tcgatccata tttacggcac gctgcgcggg cgggcgctgg cggggacggc ggggaatacg agtgcgcgaa ttttctgtcg caagctcgaa gcggaattgg ttgccatcga tggtctctac aagaccgccg aggatctgga gccgaggttc cgcggacagg ccgtccagct ctggctcgac ggcgattaca tgatgattga tacattgagc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BrabA.12891.a.A1.PW27679
PURIFICATION DATe: 1/4/2010
CONCENTRATION: 20.36mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLPLTKMNQ VLTETRPIRL KGRSFLAMVL SPELPLDGWL ERLDDLARRS SGFFLGRPVV LDMENLAIER AQLVYLLQAL NDRGVWIMGV EGARPSLLGP GMPPAMRGGQ PAADFEAPAG EPQANPGAPE PQISQAVRAP GHAVHAMPSM VITEPVRSGQ SVYFPEGDVT IVGSVASGAE VVAGGSIHIY GTLRGRALAG TAGNTSARIF CRKLEAELVA IDGLYKTAED LEPRFRGQAV QLWLDGDYMM IDTLS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgc cgttaaccaa gatgaatcaa gtgctaacag aaactcgccc gatccgcctt aaagggcggt cctttcttgc aatggtgctc tcgccggaac ttccgctcga cggctggctg gagcgcctgg acgatctcgc ccggcgttcc tccggttttt tcctcgggcg cccggttgtg ctcgatatgg aaaaccttgc catcgaacgt gcgcagctcg tctatctgtt gcaggcgttg aacgatcgcg gcgtctggat catgggcgtg gaaggcgcgc ggccttcgct tctcgggccg ggaatgccgc ctgccatgcg cggcggccag cccgcagccg attttgaagc acccgctggt gagccgcagg caaatcctgg cgcgccggaa ccgcagattt cgcaagccgt gcgtgcgcca ggccatgccg tgcacgccat gccttcgatg gttatcaccg agccggtccg ttccggccag tcggtctatt tcccggaagg ggatgtcaca attgtcggtt cggttgcttc gggtgcggaa gtggtcgcag gcggttcgat ccatatttac ggcacgctgc gcgggcgggc gctggcgggg acggcgggga atacgagtgc gcgaattttc tgtcgcaagc tcgaagcgga attggttgcc atcgatggtc tctacaagac cgccgaggat ctggagccga ggttccgcgg acaggccgtc cagctctggc tcgacggcga ttacatgatg attgatacat tgagctaata a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLPLTKMNQ VLTETRPIRL KGRSFLAMVL SPELPLDGWL ERLDDLARRS SGFFLGRPVV LDMENLAIER AQLVYLLQAL NDRGVWIMGV EGARPSLLGP GMPPAMRGGQ PAADFEAPAG EPQANPGAPE PQISQAVRAP GHAVHAMPSM VITEPVRSGQ SVYFPEGDVT IVGSVASGAE VVAGGSIHIY GTLRGRALAG TAGNTSARIF CRKLEAELVA IDGLYKTAED LEPRFRGQAV QLWLDGDYMM IDTLS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGCCGTTA ACCAAGATGA ATCAAGTGCT AACAGAAACT CGCCCGATCC GCCTTAAAGG GCGGTCCTTT CTTGCAATGG TGCTCTCGCC GGAACTTCCG CTCGACGGCT GGCTGGAGCG CCTGGACGAT CTCGCCCGGC GTTCCTCCGG TTTTTTCCTC GGGCGCCCGG TTGTGCTCGA TATGGAAAAC CTTGCCATCG AACGTGCGCA GCTCGTCTAT CTGTTGCAGG CGTTGAACGA TCGCGGCGTC TGGATCATGG GCGTGGAAGG CGCGCGGCCT TCGCTTCTCG GGCCGGGAAT GCCGCCTGCC ATGCGCGGCG GCCAGCCCGC AGCCGATTTT GAAGCACCCG CTGGTGAGCC GCAGGCAAAT CCTGGCGCGC CGGAACCGCA GATTTCGCAA GCCGTGCGTG CGCCAGGCCA TGCCGTGCAC GCCATGCCTT CGATGGTTAT CACCGAGCCG GTCCGTTCCG GCCAGTCGGT CTATTTCCCG GAAGGGGATG TCACAATTGT CGGTTCGGTT GCTTCGGGTG CGGAAGTGGT CGCAGGCGGT TCGATCCATA TTTACGGCAC GCTGCGCGGG CGGGCGCTGG CGGGGACGGC GGGGAATACG AGTGCGCGAA TTTTCTGTCG CAAGCTCGAA GCGGAATTGG TTGCCATCGA TGGTCTCTAC AAGACCGCCG AGGATCTGGA GCCGAGGTTC CGCGGACAGG CCGTCCAGCT CTGGCTCGAC GGCGATTACA TGATGATTGA TACATTGAGC AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA
Details for BrabA.12891.a.A1.PW28562
PURIFICATION DATe: 1/4/2010
CONCENTRATION: 28.94mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLPLTKMNQ VLTETRPIRL KGRSFLAMVL SPELPLDGWL ERLDDLARRS SGFFLGRPVV LDMENLAIER AQLVYLLQAL NDRGVWIMGV EGARPSLLGP GMPPAMRGGQ PAADFEAPAG EPQANPGAPE PQISQAVRAP GHAVHAMPSM VITEPVRSGQ SVYFPEGDVT IVGSVASGAE VVAGGSIHIY GTLRGRALAG TAGNTSARIF CRKLEAELVA IDGLYKTAED LEPRFRGQAV QLWLDGDYMM IDTLS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgc cgttaaccaa gatgaatcaa gtgctaacag aaactcgccc gatccgcctt aaagggcggt cctttcttgc aatggtgctc tcgccggaac ttccgctcga cggctggctg gagcgcctgg acgatctcgc ccggcgttcc tccggttttt tcctcgggcg cccggttgtg ctcgatatgg aaaaccttgc catcgaacgt gcgcagctcg tctatctgtt gcaggcgttg aacgatcgcg gcgtctggat catgggcgtg gaaggcgcgc ggccttcgct tctcgggccg ggaatgccgc ctgccatgcg cggcggccag cccgcagccg attttgaagc acccgctggt gagccgcagg caaatcctgg cgcgccggaa ccgcagattt cgcaagccgt gcgtgcgcca ggccatgccg tgcacgccat gccttcgatg gttatcaccg agccggtccg ttccggccag tcggtctatt tcccggaagg ggatgtcaca attgtcggtt cggttgcttc gggtgcggaa gtggtcgcag gcggttcgat ccatatttac ggcacgctgc gcgggcgggc gctggcgggg acggcgggga atacgagtgc gcgaattttc tgtcgcaagc tcgaagcgga attggttgcc atcgatggtc tctacaagac cgccgaggat ctggagccga ggttccgcgg acaggccgtc cagctctggc tcgacggcga ttacatgatg attgatacat tgagctaata a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLPLTKMNQ VLTETRPIRL KGRSFLAMVL SPELPLDGWL ERLDDLARRS SGFFLGRPVV LDMENLAIER AQLVYLLQAL NDRGVWIMGV EGARPSLLGP GMPPAMRGGQ PAADFEAPAG EPQANPGAPE PQISQAVRAP GHAVHAMPSM VITEPVRSGQ SVYFPEGDVT IVGSVASGAE VVAGGSIHIY GTLRGRALAG TAGNTSARIF CRKLEAELVA IDGLYKTAED LEPRFRGQAV QLWLDGDYMM IDTLS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGCCGTTA ACCAAGATGA ATCAAGTGCT AACAGAAACT CGCCCGATCC GCCTTAAAGG GCGGTCCTTT CTTGCAATGG TGCTCTCGCC GGAACTTCCG CTCGACGGCT GGCTGGAGCG CCTGGACGAT CTCGCCCGGC GTTCCTCCGG TTTTTTCCTC GGGCGCCCGG TTGTGCTCGA TATGGAAAAC CTTGCCATCG AACGTGCGCA GCTCGTCTAT CTGTTGCAGG CGTTGAACGA TCGCGGCGTC TGGATCATGG GCGTGGAAGG CGCGCGGCCT TCGCTTCTCG GGCCGGGAAT GCCGCCTGCC ATGCGCGGCG GCCAGCCCGC AGCCGATTTT GAAGCACCCG CTGGTGAGCC GCAGGCAAAT CCTGGCGCGC CGGAACCGCA GATTTCGCAA GCCGTGCGTG CGCCAGGCCA TGCCGTGCAC GCCATGCCTT CGATGGTTAT CACCGAGCCG GTCCGTTCCG GCCAGTCGGT CTATTTCCCG GAAGGGGATG TCACAATTGT CGGTTCGGTT GCTTCGGGTG CGGAAGTGGT CGCAGGCGGT TCGATCCATA TTTACGGCAC GCTGCGCGGG CGGGCGCTGG CGGGGACGGC GGGGAATACG AGTGCGCGAA TTTTCTGTCG CAAGCTCGAA GCGGAATTGG TTGCCATCGA TGGTCTCTAC AAGACCGCCG AGGATCTGGA GCCGAGGTTC CGCGGACAGG CCGTCCAGCT CTGGCTCGAC GGCGATTACA TGATGATTGA TACATTGAGC AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA