BrabA.12797.a

ROK family

CENTER ID: BrabA.12797.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.12797.a.A1.GE27790 Full length( BrabA.12797.a ) 1 374
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.12797.a.A1.PS00471 Full length( BrabA.12797.a ) 1 374
BrabA.12797.a.A1.PS00472 Full length( BrabA.12797.a ) 1 374

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.3454
RefSeq: YP_419271.1
UniProt: Q2YJB3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSIHLGMVLK ILRDEGPQSR AELARRCGLS ATTLTHLTAQ LLKDGCIAET DAPVLPSGSP MPGRPPVSVR LVPSAHYVAG VHIGAEAVQV AICDLTAQLV TSAAFDHAAN DNPLKLTHQT ADVLRNLVQA KGISLGSLVG IGMGVPGPVD QVRRRNLLSI NTGWRDVAFA DAIEAELNIP TVVEHNVTAM ALAEAHYGIG QGCPAVLYVY LGTGLGAGLV VDGMPFRPGG HGAVELGHIQ IDPQGALCAC GNRGCLETFV SERVLRERGA GSAEPLLVAL ARNPALHDEV AGHFTTALAN AVNLLTPDLI VLGGHFAEAP EAFYARLRCD LPPRVLPHMR DVLRIERGGF GNNAGAVGAA AVALDHLFYS GVSR
NT Sequence
ATGAGTATAC ACCTTGGCAT GGTGTTGAAA ATCCTGCGTG ACGAGGGGCC GCAATCGCGG GCCGAGCTGG CGAGGCGTTG CGGATTGTCG GCCACGACCC TCACGCATCT CACGGCGCAA CTGCTCAAAG ATGGTTGCAT TGCCGAAACC GACGCTCCAG TTCTGCCTTC GGGATCACCG ATGCCGGGCA GGCCTCCAGT CAGTGTGCGT CTGGTTCCCT CGGCTCATTA TGTGGCTGGC GTGCATATTG GCGCCGAAGC TGTGCAGGTG GCGATTTGCG ATCTGACGGC GCAACTGGTC ACCAGCGCTG CTTTCGATCA TGCCGCAAAC GATAATCCGC TGAAACTGAC GCATCAGACA GCCGATGTTC TGCGCAATCT CGTGCAGGCC AAAGGCATTT CATTGGGTAG CCTCGTCGGT ATCGGCATGG GGGTGCCAGG ACCGGTCGAT CAGGTGCGCC GCCGCAATTT GCTGTCAATC AATACCGGCT GGCGCGATGT CGCTTTTGCC GATGCAATTG AAGCGGAACT GAATATTCCA ACTGTGGTTG AGCATAATGT TACAGCCATG GCGCTTGCCG AGGCCCATTA CGGCATAGGG CAGGGCTGCC CGGCTGTTCT TTATGTCTAT CTTGGTACGG GGCTGGGAGC GGGGCTGGTG GTGGACGGCA TGCCGTTTCG CCCCGGCGGT CATGGTGCGG TGGAGCTAGG ACATATCCAG ATCGATCCGC AAGGTGCGCT TTGCGCCTGC GGCAATCGCG GCTGTCTGGA GACCTTTGTG TCGGAACGCG TGTTGCGCGA GCGTGGCGCT GGCAGCGCAG AGCCGCTTCT TGTGGCGCTC GCCCGTAACC CGGCATTGCA TGATGAAGTG GCAGGCCACT TTACAACTGC GCTCGCCAAT GCGGTCAATC TGCTGACACC GGATTTGATC GTGCTCGGGG GGCATTTTGC GGAAGCGCCA GAAGCTTTCT ACGCGCGTTT GCGGTGCGAT CTGCCGCCAC GGGTGCTGCC GCATATGCGC GATGTCTTGC GGATAGAGCG CGGCGGTTTC GGGAACAATG CTGGCGCAGT GGGCGCGGCT GCCGTCGCAC TGGACCACCT GTTTTATTCG GGAGTATCCC GA
Details for BrabA.12797.a.A1.GE27790
HARVESTED ON: 11/16/2009
SEQUENCED ON: 11/23/2009
EXPECTED MW: 41kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSIHLGMVL KILRDEGPQS RAELARRCGL SATTLTHLTA QLLKDGCIAE TDAPVLPSGS PMPGRPPVSV RLVPSAHYVA GVHIGAEAVQ VAICDLTAQL VTSAAFDHAA NDNPLKLTHQ TADVLRNLVQ AKGISLGSLV GIGMGVPGPV DQVRRRNLLS INTGWRDVAF ADAIEAELNI PTVVEHNVTA MALAEAHYGI GQGCPAVLYV YLGTGLGAGL VVDGMPFRPG GHGAVELGHI QIDPQGALCA CGNRGCLETF VSERVLRERG AGSAEPLLVA LARNPALHDE VAGHFTTALA NAVNLLTPDL IVLGGHFAEA PEAFYARLRC DLPPRVLPHM RDVLRIERGG FGNNAGAVGA AAVALDHLFY SGVSR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagta tacaccttgg catggtgttg aaaatcctgc gtgacgaggg gccgcaatcg cgggccgagc tggcgaggcg ttgcggattg tcggccacga ccctcacgca tctcacggcg caactgctca aagatggttg cattgccgaa accgacgctc cagttctgcc ttcgggatca ccgatgccgg gcaggcctcc agtcagtgtg cgtctggttc cctcggctca ttatgtggct ggcgtgcata ttggcgccga agctgtgcag gtggcgattt gcgatctgac ggcgcaactg gtcaccagcg ctgctttcga tcatgccgca aacgataatc cgctgaaact gacgcatcag acagccgatg ttctgcgcaa tctcgtgcag gccaaaggca tttcattggg tagcctcgtc ggtatcggca tgggggtgcc aggaccggtc gatcaggtgc gccgccgcaa tttgctgtca atcaataccg gctggcgcga tgtcgctttt gccgatgcaa ttgaagcaga actgaatatt ccaactgtgg ttgagcataa tgttacagcc atggcgcttg ccgaggccca ttacggcata gggcagggct gcccggctgt tctttatgtc tatcttggta cggggctggg agcggggctg gtggtggacg gcatgccgtt tcgccccggc ggtcatggtg cggtggagct aggacatatc cagatcgatc cgcaaggtgc gctttgcgcc tgcggcaatc gcggctgtct ggagaccttt gtgtcggaac gcgtgttgcg cgagcgtggc gctggcagcg cagagccgct tcttgtggcg ctcgcccgta acccggcatt gcatgatgaa gtggcaggcc actttacaac tgcgctcgcc aatgcggtca atctgctgac accggatttg atcgtgctcg gggggcattt tgcggaagcg ccagaagctt tctacgcgcg tttgcggtgc gatctgccgc cacgggtgct gccgcatatg cgcgatgtct tgcggataga gcgcggcggt ttcgggaaca atgctggcgc agtgggcgcg gctgccgtcg cactggacca cctgttttat tcgggagtat cccgata
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSIHLGMVL KILRDEGPQS RAELARRCGL SATTLTHLTA QLLKDGCIAE TDAPVLPSGS PMPGRPPVSV RLVPSAHYVA GVHIGAEAVQ VAICDLTAQL VTSAAFDHAA NDNPLKLTHQ TADVLRNLVQ AKGISLGSLV GIGMGVPGPV DQVRRRNLLS INTGWRDVAF ADAIEAELNI PTVVEHNVTA MALAEAHYGI GQGCPAVLYV YLGTGLGAGL VVDGMPFRPG GHGAVELGHI QIDPQGALCA CGNRGCLETF VSERVLRERG AGSAEPLLVA LARNPALHDE VAGHFTTALA NAVNLLTPDL IVLGGHFAEA PEAFYARLRC DLPPRVLPHM RDVLRIERGG FGNNAGAVGA AAVALDHLFY SGVSR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtatacac cttggcatgg tgttgaaaat cctgcgtgac gaggggccgc aatcgcgggc cgagctggcg aggcgttgcg gattgtcggc cacgaccctc acgcatctca cggcgcaact gctcaaagat ggttgcattg ccgaaaccga cgctccagtt ctgccttcgg gatcaccgat gccgggcagg cctccagtca gtgtgcgtct ggttccctcg gctcattatg tggctggcgt gcatattggc gccgaagctg tgcaggtggc gatttgcgat ctgacggcgc aactggtcac cagcgctgct ttcgatcatg ccgcaaacga taatccgctg aaactgacgc atcagacagc cgatgttctg cgcaatctcg tgcaggccaa aggcatttca ttgggtagcc tcgtcggtat cggcatgggg gtgccaggac cggtcgatca ggtgcgccgc cgcaatttgc tgtcaatcaa taccggctgg cgcgatgtcg cttttgccga tgcaattgaa gcggaactga atattccaac tgtggttgag cataatgtta cagccatggc gcttgccgag gcccattacg gcatagggca gggctgcccg gctgttcttt atgtctatct tggtacgggg ctgggagcgg ggctggtggt ggacggcatg ccgtttcgcc ccggcggtca tggtgcggtg gagctaggac atatccagat cgatccgcaa ggtgcgcttt gcgcctgcgg caatcgcggc tgtctggaga cctttgtgtc ggaacgcgtg ttgcgcgagc gtggcgctgg cagcgcagag ccgcttcttg tggcgctcgc ccgtaacccg gcattgcatg atgaagtggc aggccacttt acaactgcgc tcgccaatgc ggtcaatctg ctgacaccgg atttgatcgt gctcgggggg cattttgcgg aagcgccaga agctttctac gcgcgtttgc ggtgcgatct gccgccacgg gtgctgccgc atatgcgcga tgtcttgcgg atagagcgcg gcggtttcgg gaacaatgct ggcgcagtgg gcgcggctgc cgtcgcactg gaccacctgt tttattcggg agtatcccga aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BrabA.12797.a.A1.PS00471
PURIFICATION DATe: 1/29/2010
CONCENTRATION: 42.69mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSIHLGMVL KILRDEGPQS RAELARRCGL SATTLTHLTA QLLKDGCIAE TDAPVLPSGS PMPGRPPVSV RLVPSAHYVA GVHIGAEAVQ VAICDLTAQL VTSAAFDHAA NDNPLKLTHQ TADVLRNLVQ AKGISLGSLV GIGMGVPGPV DQVRRRNLLS INTGWRDVAF ADAIEAELNI PTVVEHNVTA MALAEAHYGI GQGCPAVLYV YLGTGLGAGL VVDGMPFRPG GHGAVELGHI QIDPQGALCA CGNRGCLETF VSERVLRERG AGSAEPLLVA LARNPALHDE VAGHFTTALA NAVNLLTPDL IVLGGHFAEA PEAFYARLRC DLPPRVLPHM RDVLRIERGG FGNNAGAVGA AAVALDHLFY SGVSR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagta tacaccttgg catggtgttg aaaatcctgc gtgacgaggg gccgcaatcg cgggccgagc tggcgaggcg ttgcggattg tcggccacga ccctcacgca tctcacggcg caactgctca aagatggttg cattgccgaa accgacgctc cagttctgcc ttcgggatca ccgatgccgg gcaggcctcc agtcagtgtg cgtctggttc cctcggctca ttatgtggct ggcgtgcata ttggcgccga agctgtgcag gtggcgattt gcgatctgac ggcgcaactg gtcaccagcg ctgctttcga tcatgccgca aacgataatc cgctgaaact gacgcatcag acagccgatg ttctgcgcaa tctcgtgcag gccaaaggca tttcattggg tagcctcgtc ggtatcggca tgggggtgcc aggaccggtc gatcaggtgc gccgccgcaa tttgctgtca atcaataccg gctggcgcga tgtcgctttt gccgatgcaa ttgaagcaga actgaatatt ccaactgtgg ttgagcataa tgttacagcc atggcgcttg ccgaggccca ttacggcata gggcagggct gcccggctgt tctttatgtc tatcttggta cggggctggg agcggggctg gtggtggacg gcatgccgtt tcgccccggc ggtcatggtg cggtggagct aggacatatc cagatcgatc cgcaaggtgc gctttgcgcc tgcggcaatc gcggctgtct ggagaccttt gtgtcggaac gcgtgttgcg cgagcgtggc gctggcagcg cagagccgct tcttgtggcg ctcgcccgta acccggcatt gcatgatgaa gtggcaggcc actttacaac tgcgctcgcc aatgcggtca atctgctgac accggatttg atcgtgctcg gggggcattt tgcggaagcg ccagaagctt tctacgcgcg tttgcggtgc gatctgccgc cacgggtgct gccgcatatg cgcgatgtct tgcggataga gcgcggcggt ttcgggaaca atgctggcgc agtgggcgcg gctgccgtcg cactggacca cctgttttat tcgggagtat cccgata
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSIHLGMVL KILRDEGPQS RAELARRCGL SATTLTHLTA QLLKDGCIAE TDAPVLPSGS PMPGRPPVSV RLVPSAHYVA GVHIGAEAVQ VAICDLTAQL VTSAAFDHAA NDNPLKLTHQ TADVLRNLVQ AKGISLGSLV GIGMGVPGPV DQVRRRNLLS INTGWRDVAF ADAIEAELNI PTVVEHNVTA MALAEAHYGI GQGCPAVLYV YLGTGLGAGL VVDGMPFRPG GHGAVELGHI QIDPQGALCA CGNRGCLETF VSERVLRERG AGSAEPLLVA LARNPALHDE VAGHFTTALA NAVNLLTPDL IVLGGHFAEA PEAFYARLRC DLPPRVLPHM RDVLRIERGG FGNNAGAVGA AAVALDHLFY SGVSR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGTATACAC CTTGGCATGG TGTTGAAAAT CCTGCGTGAC GAGGGGCCGC AATCGCGGGC CGAGCTGGCG AGGCGTTGCG GATTGTCGGC CACGACCCTC ACGCATCTCA CGGCGCAACT GCTCAAAGAT GGTTGCATTG CCGAAACCGA CGCTCCAGTT CTGCCTTCGG GATCACCGAT GCCGGGCAGG CCTCCAGTCA GTGTGCGTCT GGTTCCCTCG GCTCATTATG TGGCTGGCGT GCATATTGGC GCCGAAGCTG TGCAGGTGGC GATTTGCGAT CTGACGGCGC AACTGGTCAC CAGCGCTGCT TTCGATCATG CCGCAAACGA TAATCCGCTG AAACTGACGC ATCAGACAGC CGATGTTCTG CGCAATCTCG TGCAGGCCAA AGGCATTTCA TTGGGTAGCC TCGTCGGTAT CGGCATGGGG GTGCCAGGAC CGGTCGATCA GGTGCGCCGC CGCAATTTGC TGTCAATCAA TACCGGCTGG CGCGATGTCG CTTTTGCCGA TGCAATTGAA GCGGAACTGA ATATTCCAAC TGTGGTTGAG CATAATGTTA CAGCCATGGC GCTTGCCGAG GCCCATTACG GCATAGGGCA GGGCTGCCCG GCTGTTCTTT ATGTCTATCT TGGTACGGGG CTGGGAGCGG GGCTGGTGGT GGACGGCATG CCGTTTCGCC CCGGCGGTCA TGGTGCGGTG GAGCTAGGAC ATATCCAGAT CGATCCGCAA GGTGCGCTTT GCGCCTGCGG CAATCGCGGC TGTCTGGAGA CCTTTGTGTC GGAACGCGTG TTGCGCGAGC GTGGCGCTGG CAGCGCAGAG CCGCTTCTTG TGGCGCTCGC CCGTAACCCG GCATTGCATG ATGAAGTGGC AGGCCACTTT ACAACTGCGC TCGCCAATGC GGTCAATCTG CTGACACCGG ATTTGATCGT GCTCGGGGGG CATTTTGCGG AAGCGCCAGA AGCTTTCTAC GCGCGTTTGC GGTGCGATCT GCCGCCACGG GTGCTGCCGC ATATGCGCGA TGTCTTGCGG ATAGAGCGCG GCGGTTTCGG GAACAATGCT GGCGCAGTGG GCGCGGCTGC CGTCGCACTG GACCACCTGT TTTATTCGGG AGTATCCCGA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA
Details for BrabA.12797.a.A1.PS00472
PURIFICATION DATe: 1/29/2010
CONCENTRATION: 27.3mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSIHLGMVL KILRDEGPQS RAELARRCGL SATTLTHLTA QLLKDGCIAE TDAPVLPSGS PMPGRPPVSV RLVPSAHYVA GVHIGAEAVQ VAICDLTAQL VTSAAFDHAA NDNPLKLTHQ TADVLRNLVQ AKGISLGSLV GIGMGVPGPV DQVRRRNLLS INTGWRDVAF ADAIEAELNI PTVVEHNVTA MALAEAHYGI GQGCPAVLYV YLGTGLGAGL VVDGMPFRPG GHGAVELGHI QIDPQGALCA CGNRGCLETF VSERVLRERG AGSAEPLLVA LARNPALHDE VAGHFTTALA NAVNLLTPDL IVLGGHFAEA PEAFYARLRC DLPPRVLPHM RDVLRIERGG FGNNAGAVGA AAVALDHLFY SGVSR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagta tacaccttgg catggtgttg aaaatcctgc gtgacgaggg gccgcaatcg cgggccgagc tggcgaggcg ttgcggattg tcggccacga ccctcacgca tctcacggcg caactgctca aagatggttg cattgccgaa accgacgctc cagttctgcc ttcgggatca ccgatgccgg gcaggcctcc agtcagtgtg cgtctggttc cctcggctca ttatgtggct ggcgtgcata ttggcgccga agctgtgcag gtggcgattt gcgatctgac ggcgcaactg gtcaccagcg ctgctttcga tcatgccgca aacgataatc cgctgaaact gacgcatcag acagccgatg ttctgcgcaa tctcgtgcag gccaaaggca tttcattggg tagcctcgtc ggtatcggca tgggggtgcc aggaccggtc gatcaggtgc gccgccgcaa tttgctgtca atcaataccg gctggcgcga tgtcgctttt gccgatgcaa ttgaagcaga actgaatatt ccaactgtgg ttgagcataa tgttacagcc atggcgcttg ccgaggccca ttacggcata gggcagggct gcccggctgt tctttatgtc tatcttggta cggggctggg agcggggctg gtggtggacg gcatgccgtt tcgccccggc ggtcatggtg cggtggagct aggacatatc cagatcgatc cgcaaggtgc gctttgcgcc tgcggcaatc gcggctgtct ggagaccttt gtgtcggaac gcgtgttgcg cgagcgtggc gctggcagcg cagagccgct tcttgtggcg ctcgcccgta acccggcatt gcatgatgaa gtggcaggcc actttacaac tgcgctcgcc aatgcggtca atctgctgac accggatttg atcgtgctcg gggggcattt tgcggaagcg ccagaagctt tctacgcgcg tttgcggtgc gatctgccgc cacgggtgct gccgcatatg cgcgatgtct tgcggataga gcgcggcggt ttcgggaaca atgctggcgc agtgggcgcg gctgccgtcg cactggacca cctgttttat tcgggagtat cccgata
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSIHLGMVL KILRDEGPQS RAELARRCGL SATTLTHLTA QLLKDGCIAE TDAPVLPSGS PMPGRPPVSV RLVPSAHYVA GVHIGAEAVQ VAICDLTAQL VTSAAFDHAA NDNPLKLTHQ TADVLRNLVQ AKGISLGSLV GIGMGVPGPV DQVRRRNLLS INTGWRDVAF ADAIEAELNI PTVVEHNVTA MALAEAHYGI GQGCPAVLYV YLGTGLGAGL VVDGMPFRPG GHGAVELGHI QIDPQGALCA CGNRGCLETF VSERVLRERG AGSAEPLLVA LARNPALHDE VAGHFTTALA NAVNLLTPDL IVLGGHFAEA PEAFYARLRC DLPPRVLPHM RDVLRIERGG FGNNAGAVGA AAVALDHLFY SGVSR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGTATACAC CTTGGCATGG TGTTGAAAAT CCTGCGTGAC GAGGGGCCGC AATCGCGGGC CGAGCTGGCG AGGCGTTGCG GATTGTCGGC CACGACCCTC ACGCATCTCA CGGCGCAACT GCTCAAAGAT GGTTGCATTG CCGAAACCGA CGCTCCAGTT CTGCCTTCGG GATCACCGAT GCCGGGCAGG CCTCCAGTCA GTGTGCGTCT GGTTCCCTCG GCTCATTATG TGGCTGGCGT GCATATTGGC GCCGAAGCTG TGCAGGTGGC GATTTGCGAT CTGACGGCGC AACTGGTCAC CAGCGCTGCT TTCGATCATG CCGCAAACGA TAATCCGCTG AAACTGACGC ATCAGACAGC CGATGTTCTG CGCAATCTCG TGCAGGCCAA AGGCATTTCA TTGGGTAGCC TCGTCGGTAT CGGCATGGGG GTGCCAGGAC CGGTCGATCA GGTGCGCCGC CGCAATTTGC TGTCAATCAA TACCGGCTGG CGCGATGTCG CTTTTGCCGA TGCAATTGAA GCGGAACTGA ATATTCCAAC TGTGGTTGAG CATAATGTTA CAGCCATGGC GCTTGCCGAG GCCCATTACG GCATAGGGCA GGGCTGCCCG GCTGTTCTTT ATGTCTATCT TGGTACGGGG CTGGGAGCGG GGCTGGTGGT GGACGGCATG CCGTTTCGCC CCGGCGGTCA TGGTGCGGTG GAGCTAGGAC ATATCCAGAT CGATCCGCAA GGTGCGCTTT GCGCCTGCGG CAATCGCGGC TGTCTGGAGA CCTTTGTGTC GGAACGCGTG TTGCGCGAGC GTGGCGCTGG CAGCGCAGAG CCGCTTCTTG TGGCGCTCGC CCGTAACCCG GCATTGCATG ATGAAGTGGC AGGCCACTTT ACAACTGCGC TCGCCAATGC GGTCAATCTG CTGACACCGG ATTTGATCGT GCTCGGGGGG CATTTTGCGG AAGCGCCAGA AGCTTTCTAC GCGCGTTTGC GGTGCGATCT GCCGCCACGG GTGCTGCCGC ATATGCGCGA TGTCTTGCGG ATAGAGCGCG GCGGTTTCGG GAACAATGCT GGCGCAGTGG GCGCGGCTGC CGTCGCACTG GACCACCTGT TTTATTCGGG AGTATCCCGA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA