BrabA.11339.a

Beta-lactamase-like

CENTER ID: BrabA.11339.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.11339.a.A1.GE27785 Full length( BrabA.11339.a ) 1 272
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.11339.a.A1.PW27673 Full length( BrabA.11339.a ) 1 272
Structures
3MD7
DEPOSITED: 3/30/2010
DETERMINATION: XRay
CLONE: BrabA.11339.a.A1.GE27785
PROTEIN: BrabA.11339.a.A1.PW27673
3PY5
DEPOSITED: 12/11/2010
DETERMINATION: XRay
CLONE: BrabA.11339.a.A1.GE27785
PROTEIN: BrabA.11339.a.A1.PW27673
3PY6
DEPOSITED: 12/11/2010
DETERMINATION: XRay
CLONE: BrabA.11339.a.A1.GE27785
PROTEIN: BrabA.11339.a.A1.PW27673
3QH8
DEPOSITED: 1/25/2011
DETERMINATION: XRay
CLONE: BrabA.11339.a.A1.GE27785
PROTEIN: BrabA.11339.a.A1.PW27673
Publications by SSGCID
BrabA.11339.a: anomalous diffraction and ligand binding guide towards the elucidation of the function of a 'putative beta-lactamase-like protein' from Brucella melitensis.
Abendroth J, Bhandari J, Dieterich S, Edwards TE, Gardberg AS, Myler PJ, Napuli AJ, Sankaran B, Staker BL, Stewart LJ, Van Voorhis WC
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2011
volume 67, issue Pt 9, pages 1106-12
PMID: 21904058; PMCID: PMC3169410
PDB: 3MD7, 3PY5, 3PY6, 3QH8
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_129512
PATRIC ID: fig|359391.4.peg.1061
RefSeq: YP_414422.1
UniProt: Q2YQ74
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSPRNCLRF TLLGCGSSPG VPRINGDWGK CDPKNPKNRR RRASLLVERY DAEGNNTVVV IDTGPDFRMQ MIDSGVHMLD AAVYTHPHAD HIHGIDDLRT YVVDNGRLMD VYANRLTRNR LYDTFGYCFE TPVGSSYPPI LSMHDIAPET PFSIEGAGGA IRFEPFSQVH GDIESLGFRI GSVVYCTDVS AFPEQSLQYI KDADVLIIGA LQYRPHPSHF SLGEALEWIE KLSPKRAILT HMHVPLDYET VMRETPHHVE PGYDGLRFEV AV
NT Sequence
GTGACTTCTC CCCGCAATTG CCTGCGTTTC ACATTGCTTG GCTGCGGCTC GTCGCCGGGC GTGCCGCGCA TCAATGGCGA TTGGGGCAAG TGCGATCCGA AAAATCCGAA AAATCGCCGC CGCCGCGCTT CTCTGCTTGT GGAACGCTAT GATGCGGAGG GAAACAATAC GGTTGTCGTA ATCGACACCG GCCCGGATTT TCGCATGCAG ATGATTGATT CTGGCGTGCA TATGCTGGAT GCGGCGGTTT ACACCCATCC CCATGCCGAC CACATTCACG GTATTGATGA TCTGCGTACC TATGTGGTCG ATAATGGGCG CCTGATGGAT GTCTATGCCA ACCGGCTGAC GCGCAACCGT CTCTACGACA CATTCGGTTA TTGTTTTGAA ACGCCCGTCG GGTCGAGTTA CCCGCCGATC CTCTCGATGC ACGATATCGC GCCGGAAACG CCCTTTTCGA TTGAGGGCGC GGGCGGCGCC ATCCGCTTCG AGCCGTTCAG CCAGGTGCAT GGCGATATTG AATCGCTGGG GTTCCGTATC GGCAGCGTTG TCTATTGCAC GGATGTCAGC GCATTTCCCG AGCAAAGCCT GCAATATATA AAGGACGCGG ATGTTCTCAT CATCGGCGCC CTGCAATATC GTCCGCACCC AAGCCATTTT TCGCTTGGGG AAGCGCTGGA GTGGATCGAG AAACTATCGC CGAAGCGCGC TATTCTCACC CATATGCATG TACCGCTCGA TTATGAAACG GTGATGCGGG AAACGCCGCA TCACGTGGAG CCGGGCTATG ACGGCCTTCG CTTTGAAGTG GCCGTT
Details for BrabA.11339.a.A1.GE27785
HARVESTED ON: 11/16/2009
SEQUENCED ON: 11/23/2009
EXPECTED MW: 32kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTSPRNCL RFTLLGCGSS PGVPRINGDW GKCDPKNPKN RRRRASLLVE RYDAEGNNTV VVIDTGPDFR MQMIDSGVHM LDAAVYTHPH ADHIHGIDDL RTYVVDNGRL MDVYANRLTR NRLYDTFGYC FETPVGSSYP PILSMHDIAP ETPFSIEGAG GAIRFEPFSQ VHGDIESLGF RIGSVVYCTD VSAFPEQSLQ YIKDADVLII GALQYRPHPS HFSLGEALEW IEKLSPKRAI LTHMHVPLDY ETVMRETPHH VEPGYDGLRF EVAV
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga cttctccccg caattgcctg cgtttcacat tgcttggctg cggctcgtcg ccgggcgtgc cgcgcatcaa tggcgattgg ggcaagtgcg atccgaaaaa tccgaaaaat cgccgccgcc gcgcttctct gcttgtggaa cgctatgatg cggagggaaa caatacggtt gtcgtaatcg acaccggccc ggattttcgc atgcagatga ttgattctgg cgtgcatatg ctggatgcgg cggtttacac ccatccccat gccgaccaca ttcacggtat tgatgatctg cgtacctatg tggtcgataa tgggcgcctg atggatgtct atgccaaccg gctgacgcgc aaccgtctct acgacacatt cggttattgt tttgaaacgc ccgtcgggtc gagttacccg ccgatcctct cgatgcacga tatcgcgccg gaaacgccct tttcgattga gggcgcgggc ggcgccatcc gcttcgagcc gttcagccag gtgcatggcg atattgaatc gctggggttc cgtatcggca gcgttgtcta ttgcacggat gtcagcgcat ttcccgagca aagcctgcaa tatataaagg acgcggatgt tctcatcatc ggcgccctgc aatatcgtcc gcacccaagc catttttcgc ttggggaagc gctggagtgg atcgagaaac tatcgccgaa gcgcgctatt ctcacccata tgcatgtacc gctcgattat gaaacggtga tgcgggaaac gccgcatcac gtggagccgg gctatgacgg ccttcgcttt gaagtggccg tttaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTSPRNCL RFTLLGCGSS PGVPRINGDW GKCDPKNPKN RRRRASLLVE RYDAEGNNTV VVIDTGPDFR MQMIDSGVHM LDAAVYTHPH ADHIHGIDDL RTYVVDNGRL MDVYANRLTR NRLYDTFGYC FETPVGSSYP PILSMHDIAP ETPFSIEGAG GAIRFEPFSQ VHGDIESLGF RIGSVVYCTD VSAFPEQSLQ YIKDADVLII GALQYRPHPS HFSLGEALEW IEKLSPKRAI LTHMHVPLDY ETVMRETPHH VEPGYDGLRF EVAV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacttct ccccgcaatt gcctgcgttt cacattgctt ggctgcggct cgtcgccggg cgtgccgcgc atcaatggcg attggggcaa gtgcgatccg aaaaatccga aaaatcgccg ccgccgcgct tctctgcttg tggaacgcta tgatgcggag ggaaacaata cggttgtcgt aatcgacacc ggcccggatt ttcgcatgca gatgattgat tctggcgtgc atatgctgga tgcggcggtt tacacccatc cccatgccga ccacattcac ggtattgatg atctgcgtac ctatgtggtc gataatgggc gcctgatgga tgtctatgcc aaccggctga cgcgcaaccg tctctacgac acattcggtt attgttttga aacgcccgtc gggtcgagtt acccgccgat cctctcgatg cacgatatcg cgccggaaac gcccttttcg attgagggcg cgggcggcgc catccgcttc gagccgttca gccaggtgca tggcgatatt gaatcgctgg ggttccgtat cggcagcgtt gtctattgca cggatgtcag cgcatttccc gagcaaagcc tgcaatatat aaaggacgcg gatgttctca tcatcggcgc cctgcaatat cgtccgcacc caagccattt ttcgcttggg gaagcgctgg agtggatcga gaaactatcg ccgaagcgcg ctattctcac ccatatgcat gtaccgctcg attatgaaac ggtgatgcgg gaaacgccgc atcacgtgga gccgggctat gacggccttc gctttgaagt ggccgttaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BrabA.11339.a.A1.PW27673
PURIFICATION DATe: 1/4/2010
CONCENTRATION: 19.59mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTSPRNCL RFTLLGCGSS PGVPRINGDW GKCDPKNPKN RRRRASLLVE RYDAEGNNTV VVIDTGPDFR MQMIDSGVHM LDAAVYTHPH ADHIHGIDDL RTYVVDNGRL MDVYANRLTR NRLYDTFGYC FETPVGSSYP PILSMHDIAP ETPFSIEGAG GAIRFEPFSQ VHGDIESLGF RIGSVVYCTD VSAFPEQSLQ YIKDADVLII GALQYRPHPS HFSLGEALEW IEKLSPKRAI LTHMHVPLDY ETVMRETPHH VEPGYDGLRF EVAV
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga cttctccccg caattgcctg cgtttcacat tgcttggctg cggctcgtcg ccgggcgtgc cgcgcatcaa tggcgattgg ggcaagtgcg atccgaaaaa tccgaaaaat cgccgccgcc gcgcttctct gcttgtggaa cgctatgatg cggagggaaa caatacggtt gtcgtaatcg acaccggccc ggattttcgc atgcagatga ttgattctgg cgtgcatatg ctggatgcgg cggtttacac ccatccccat gccgaccaca ttcacggtat tgatgatctg cgtacctatg tggtcgataa tgggcgcctg atggatgtct atgccaaccg gctgacgcgc aaccgtctct acgacacatt cggttattgt tttgaaacgc ccgtcgggtc gagttacccg ccgatcctct cgatgcacga tatcgcgccg gaaacgccct tttcgattga gggcgcgggc ggcgccatcc gcttcgagcc gttcagccag gtgcatggcg atattgaatc gctggggttc cgtatcggca gcgttgtcta ttgcacggat gtcagcgcat ttcccgagca aagcctgcaa tatataaagg acgcggatgt tctcatcatc ggcgccctgc aatatcgtcc gcacccaagc catttttcgc ttggggaagc gctggagtgg atcgagaaac tatcgccgaa gcgcgctatt ctcacccata tgcatgtacc gctcgattat gaaacggtga tgcgggaaac gccgcatcac gtggagccgg gctatgacgg ccttcgcttt gaagtggccg tttaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTSPRNCL RFTLLGCGSS PGVPRINGDW GKCDPKNPKN RRRRASLLVE RYDAEGNNTV VVIDTGPDFR MQMIDSGVHM LDAAVYTHPH ADHIHGIDDL RTYVVDNGRL MDVYANRLTR NRLYDTFGYC FETPVGSSYP PILSMHDIAP ETPFSIEGAG GAIRFEPFSQ VHGDIESLGF RIGSVVYCTD VSAFPEQSLQ YIKDADVLII GALQYRPHPS HFSLGEALEW IEKLSPKRAI LTHMHVPLDY ETVMRETPHH VEPGYDGLRF EVAV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACTTCT CCCCGCAATT GCCTGCGTTT CACATTGCTT GGCTGCGGCT CGTCGCCGGG CGTGCCGCGC ATCAATGGCG ATTGGGGCAA GTGCGATCCG AAAAATCCGA AAAATCGCCG CCGCCGCGCT TCTCTGCTTG TGGAACGCTA TGATGCGGAG GGAAACAATA CGGTTGTCGT AATCGACACC GGCCCGGATT TTCGCATGCA GATGATTGAT TCTGGCGTGC ATATGCTGGA TGCGGCGGTT TACACCCATC CCCATGCCGA CCACATTCAC GGTATTGATG ATCTGCGTAC CTATGTGGTC GATAATGGGC GCCTGATGGA TGTCTATGCC AACCGGCTGA CGCGCAACCG TCTCTACGAC ACATTCGGTT ATTGTTTTGA AACGCCCGTC GGGTCGAGTT ACCCGCCGAT CCTCTCGATG CACGATATCG CGCCGGAAAC GCCCTTTTCG ATTGAGGGCG CGGGCGGCGC CATCCGCTTC GAGCCGTTCA GCCAGGTGCA TGGCGATATT GAATCGCTGG GGTTCCGTAT CGGCAGCGTT GTCTATTGCA CGGATGTCAG CGCATTTCCC GAGCAAAGCC TGCAATATAT AAAGGACGCG GATGTTCTCA TCATCGGCGC CCTGCAATAT CGTCCGCACC CAAGCCATTT TTCGCTTGGG GAAGCGCTGG AGTGGATCGA GAAACTATCG CCGAAGCGCG CTATTCTCAC CCATATGCAT GTACCGCTCG ATTATGAAAC GGTGATGCGG GAAACGCCGC ATCACGTGGA GCCGGGCTAT GACGGCCTTC GCTTTGAAGT GGCCGTTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA