SSGCID: Target Page

BrabA.10537.a

Heat shock protein Hsp70:Cell division protein FtsA

CENTER ID:	BrabA.10537.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.10537.a.A1.GE27783	Full length( BrabA.10537.a )		1	440

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.10537.a.A1.PW27671	Full length( BrabA.10537.a )		1	440

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.1502
RefSeq:	YP_414817.1
UniProt:	Q2YLZ0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSILGGHGAS QHGTAGRKVR LLTVLDVGSS KVSCVIARLR PHEGGALLPG RTHRMEVLGI GHQRSRGVKS GVIIDLDAAE QSIRLAVDAA ERMAGLTVDS LIVNISAGRL KSETFTASVN LGGHEVEQTD IRRVLAAGAK QALAAERHLV HSLPVGYTLD GERGIREPLG MLGDSLGVDM HVLTADAAPL RNLELCINRC HLSVEAIVAT PYASGLAALV GDEAEMGAAC IDMGGGTTTI SVFSEGKFIH ADAVAIGGNH VTMDVARGFS TRMEDAERLK VMYGSALPSA ADDRDLISVP PIGDDERDVP NQYPRSVLTR IIRARVEETL ELVRDRLNQS GLGHIVGKRV VLTGGASQIP GMPEAARRIL ARNVRIGRPL GIAGLPEAAK GAAFAATVGL LIYPQVAGIE ERSVKAASSG LMTGTGGRIQ RVGQWLRESF

NT Sequence

ATGAGTATTC TTGGCGGTCA TGGAGCATCA CAACACGGAA CCGCAGGGCG CAAGGTGCGC CTGTTAACGG TTCTGGACGT TGGGTCGAGC AAGGTTTCCT GTGTCATCGC AAGGCTGCGC CCGCATGAAG GCGGCGCGTT GCTGCCGGGA CGCACCCATC GCATGGAAGT GCTGGGCATC GGCCATCAGC GTTCGCGCGG CGTGAAATCC GGCGTCATTA TCGACCTTGA TGCGGCCGAG CAGTCGATCC GCCTTGCGGT AGATGCGGCC GAGCGCATGG CTGGCCTGAC CGTCGATAGC CTCATCGTGA ATATTTCGGC TGGCCGTCTC AAAAGCGAAA CCTTTACCGC AAGCGTCAAT CTTGGCGGCC ATGAAGTCGA ACAGACAGAT ATCCGCCGCG TTCTGGCTGC TGGTGCAAAA CAGGCGCTGG CTGCCGAACG TCACCTCGTT CATTCGCTCC CCGTCGGTTA TACGCTCGAC GGCGAGCGTG GCATCCGTGA GCCGCTTGGA ATGCTGGGCG ACAGCCTTGG CGTGGATATG CATGTGCTGA CGGCTGATGC GGCCCCGCTT CGCAATCTGG AGCTTTGCAT CAACCGCTGC CACCTTTCGG TGGAGGCAAT CGTTGCAACG CCTTATGCAA GTGGCCTTGC TGCCCTGGTG GGCGACGAGG CGGAAATGGG TGCGGCCTGT ATCGACATGG GCGGCGGCAC GACGACGATT TCCGTTTTTT CGGAAGGCAA GTTCATTCAT GCCGATGCGG TTGCCATCGG CGGCAATCAC GTCACGATGG ATGTGGCGCG TGGCTTTTCC ACCCGTATGG AAGACGCCGA ACGGCTGAAG GTCATGTATG GCTCCGCCCT GCCAAGTGCT GCCGACGACC GCGATCTGAT CTCCGTTCCG CCCATCGGCG ATGATGAGCG GGATGTACCC AATCAATATC CGCGCTCGGT GCTGACGCGG ATTATCCGTG CAAGAGTGGA AGAGACGCTG GAACTGGTGC GCGACAGGCT GAACCAGTCG GGCCTCGGAC ATATCGTCGG CAAGCGCGTG GTTCTGACGG GCGGCGCAAG CCAGATTCCC GGTATGCCCG AAGCGGCCAG GCGAATCCTT GCAAGAAATG TACGAATCGG GCGTCCTCTA GGAATAGCGG GATTGCCCGA GGCGGCTAAG GGGGCAGCTT TCGCGGCGAC CGTAGGCCTT CTGATTTACC CGCAGGTGGC GGGGATAGAA GAGCGTTCGG TCAAAGCAGC GTCCTCCGGT CTGATGACCG GTACGGGTGG GCGCATTCAA CGAGTCGGCC AATGGCTGAG AGAGAGTTTT

Details for BrabA.10537.a.A1.GE27783

HARVESTED ON:	11/16/2009
SEQUENCED ON:	11/23/2009
EXPECTED MW:	48kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSILGGHGA SQHGTAGRKV RLLTVLDVGS SKVSCVIARL RPHEGGALLP GRTHRMEVLG IGHQRSRGVK SGVIIDLDAA EQSIRLAVDA AERMAGLTVD SLIVNISAGR LKSETFTASV NLGGHEVEQT DIRRVLAAGA KQALAAERHL VHSLPVGYTL DGERGIREPL GMLGDSLGVD MHVLTADAAP LRNLELCINR CHLSVEAIVA TPYASGLAAL VGDEAEMGAA CIDMGGGTTT ISVFSEGKFI HADAVAIGGN HVTMDVARGF STRMEDAERL KVMYGSALPS AADDRDLISV PPIGDDERDV PNQYPRSVLT RIIRARVEET LELVRDRLNQ SGLGHIVGKR VVLTGGASQI PGMPEAARRI LARNVRIGRP LGIAGLPEAA KGAAFAATVG LLIYPQVAGI EERSVKAASS GLMTGTGGRI QRVGQWLRES F

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagta ttcttggcgg tcatggagca tcacaacacg gaaccgcagg gcgcaaggtg cgcctgttaa cggttctgga cgttgggtcg agcaaggttt cctgtgtcat cgcaaggctg cgcccgcatg aaggcggcgc gttgctgccg ggacgcaccc atcgcatgga agtgctgggc atcggccatc agcgttcgcg cggcgtgaaa tccggcgtca ttatcgacct tgatgcggcc gagcagtcga tccgccttgc ggtagatgcg gccgagcgca tggctggcct gaccgtcgat agcctcatcg tgaatatttc ggctggccgt ctcaaaagcg aaacctttac cgcaagcgtc aatcttggcg gccatgaagt cgaacagaca gatatccgcc gcgttctggc tgctggtgca aaacaggcgc tggctgccga acgtcacctc gttcattcgc tccccgtcgg ttatacgctc gacggcgagc gtggcatccg tgagccgctt ggaatgctgg gcgacagcct tggcgtggat atgcatgtgc tgacggctga tgcggccccg cttcgcaatc tggagctttg catcaaccgc tgccaccttt cggtggaggc aatcgttgca acgccttatg caagtggcct tgctgccctg gtgggcgacg aggcggaaat gggtgcggcc tgtatcgaca tgggcggcgg cacgacgacg atttccgttt tttcggaagg caagttcatt catgccgatg cggttgccat cggcggcaat cacgtcacga tggatgtggc gcgtggcttt tccacccgta tggaagacgc cgaacggctg aaggtcatgt atggctccgc cctgccaagt gctgccgacg accgcgatct gatctccgtt ccgcccatcg gcgatgatga gcgggatgta cccaatcaat atccgcgctc ggtgctgacg cggattatcc gtgcaagagt ggaagagacg ctggaactgg tgcgcgacag gctgaaccag tcgggcctcg gacatatcgt cggcaagcgc gtggttctga cgggcggcgc aagccagatt cccggtatgc ccgaagcggc caggcgaatc cttgcaagaa atgtacgaat cgggcgtcct ctaggaatag cgggattgcc cgaggcggct aagggggcag ctttcgcggc gaccgtaggc cttctgattt acccgcaggt ggcggggata gaagagcgtt cggtcaaagc agcgtcctcc ggtctgatga ccggtacggg tgggcgcatt caacgagtcg gccaatggct gagagagagt ttttaat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtattctt ggcggtcatg gagcatcaca acacggaacc gcagggcgca aggtgcgcct gttaacggtt ctggacgttg ggtcgagcaa ggtttcctgt gtcatcgcaa ggctgcgccc gcatgaaggc ggcgcgttgc tgccgggacg cacccatcgc atggaagtgc tgggcatcgg ccatcagcgt tcgcgcggcg tgaaatccgg cgtcattatc gaccttgatg cggccgagca gtcgatccgc cttgcggtag atgcggccga gcgcatggct ggcctgaccg tcgatagcct catcgtgaat atttcggctg gccgtctcaa aagcgaaacc tttaccgcaa gcgtcaatct tggcggccat gaagtcgaac agacagatat ccgccgcgtt ctggctgctg gtgcaaaaca ggcgctggct gccgaacgtc acctcgttca ttcgctcccc gtcggttata cgctcgacgg cgagcgtggc atccgtgagc cgcttggaat gctgggcgac agccttggcg tggatatgca tgtgctgacg gctgatgcgg ccccgcttcg caatctggag ctttgcatca accgctgcca cctttcggtg gaggcaatcg ttgcaacgcc ttatgcaagt ggccttgctg ccctggtggg cgacgaggcg gaaatgggtg cggcctgtat cgacatgggc ggcggcacga cgacgatttc cgttttttcg gaaggcaagt tcattcatgc cgatgcggtt gccatcggcg gcaatcacgt cacgatggat gtggcgcgtg gcttttccac ccgtatggaa gacgccgaac ggctgaaggt catgtatggc tccgccctgc caagtgctgc cgacgaccgc gatctgatct ccgttccgcc catcggcgat gatgagcggg atgtacccaa tcaatatccg cgctcggtgc tgacgcggat tatccgtgca agagtggaag agacgctgga actggtgcgc gacaggctga accagtcggg cctcggacat atcgtcggca agcgcgtggt tctgacgggc ggcgcaagcc agattcccgg tatgcccgaa gcggccaggc gaatccttgc aagaaatgta cgaatcgggc gtcctctagg aatagcggga ttgcccgagg cggctaaggg ggcagctttc gcggcgaccg taggccttct gatttacccg caggtggcgg ggatagaaga gcgttcggtc aaagcagcgt cctccggtct gatgaccggt acgggtgggc gcattcaacg agtcggccaa tggctgagag agagttttaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for BrabA.10537.a.A1.PW27671

PURIFICATION DATe:	12/28/2009
CONCENTRATION:	7.22mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGTATTCTT GGCGGTCATG GAGCATCACA ACACGGAACC GCAGGGCGCA AGGTGCGCCT GTTAACGGTT CTGGACGTTG GGTCGAGCAA GGTTTCCTGT GTCATCGCAA GGCTGCGCCC GCATGAAGGC GGCGCGTTGC TGCCGGGACG CACCCATCGC ATGGAAGTGC TGGGCATCGG CCATCAGCGT TCGCGCGGCG TGAAATCCGG CGTCATTATC GACCTTGATG CGGCCGAGCA GTCGATCCGC CTTGCGGTAG ATGCGGCCGA GCGCATGGCT GGCCTGACCG TCGATAGCCT CATCGTGAAT ATTTCGGCTG GCCGTCTCAA AAGCGAAACC TTTACCGCAA GCGTCAATCT TGGCGGCCAT GAAGTCGAAC AGACAGATAT CCGCCGCGTT CTGGCTGCTG GTGCAAAACA GGCGCTGGCT GCCGAACGTC ACCTCGTTCA TTCGCTCCCC GTCGGTTATA CGCTCGACGG CGAGCGTGGC ATCCGTGAGC CGCTTGGAAT GCTGGGCGAC AGCCTTGGCG TGGATATGCA TGTGCTGACG GCTGATGCGG CCCCGCTTCG CAATCTGGAG CTTTGCATCA ACCGCTGCCA CCTTTCGGTG GAGGCAATCG TTGCAACGCC TTATGCAAGT GGCCTTGCTG CCCTGGTGGG CGACGAGGCG GAAATGGGTG CGGCCTGTAT CGACATGGGC GGCGGCACGA CGACGATTTC CGTTTTTTCG GAAGGCAAGT TCATTCATGC CGATGCGGTT GCCATCGGCG GCAATCACGT CACGATGGAT GTGGCGCGTG GCTTTTCCAC CCGTATGGAA GACGCCGAAC GGCTGAAGGT CATGTATGGC TCCGCCCTGC CAAGTGCTGC CGACGACCGC GATCTGATCT CCGTTCCGCC CATCGGCGAT GATGAGCGGG ATGTACCCAA TCAATATCCG CGCTCGGTGC TGACGCGGAT TATCCGTGCA AGAGTGGAAG AGACGCTGGA ACTGGTGCGC GACAGGCTGA ACCAGTCGGG CCTCGGACAT ATCGTCGGCA AGCGCGTGGT TCTGACGGGC GGCGCAAGCC AGATTCCCGG TATGCCCGAA GCGGCCAGGC GAATCCTTGC AAGAAATGTA CGAATCGGGC GTCCTCTAGG AATAGCGGGA TTGCCCGAGG CGGCTAAGGG GGCAGCTTTC GCGGCGACCG TAGGCCTTCT GATTTACCCG CAGGTGGCGG GGATAGAAGA GCGTTCGGTC AAAGCAGCGT CCTCCGGTCT GATGACCGGT ACGGGTGGGC GCATTCAACG AGTCGGCCAA TGGCTGAGAG AGAGTTTTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA