BrabA.10520.a

Beta-lactamase-like

CENTER ID: BrabA.10520.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.10520.a.A1.GE27782 Full length( BrabA.10520.a ) 1 218
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.10520.a.A1.PW27670 Full length( BrabA.10520.a ) 1 218

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_127147
PATRIC ID: fig|359391.4.peg.1593
RefSeq: YP_414900.1
UniProt: Q2YRL8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGQLQAIIVP VTPFQQNCTI LFDGETKKGV VIDPGGDLAR IREAIESQGI DVEAIWITHG HIDHAAAALD LKEALGVDII GPHRDDKFLL DNLVAAGLKY GITEGVRNVT PDQWLEEGDK VCVAGHVFDV FYCPGHAPGH VVFFNPEARF AIVGDVLFNG SVGRTDLPGG DHATLIRSIK EKLLPLGDDI GFICGHGPGG RFGEERRSNP FLTDTGYA
NT Sequence
ATGGGGCAAT TGCAGGCCAT TATCGTTCCC GTCACGCCGT TTCAGCAAAA TTGCACCATT CTCTTTGATG GTGAAACCAA GAAGGGGGTG GTGATCGACC CCGGCGGCGA CCTTGCGCGC ATCAGGGAAG CCATTGAATC CCAGGGCATC GACGTCGAAG CGATCTGGAT CACGCATGGC CATATCGATC ATGCCGCTGC CGCGCTCGAT CTCAAGGAGG CGCTGGGTGT GGATATTATC GGGCCGCACA GGGACGACAA GTTTCTCCTC GATAATCTCG TGGCGGCTGG CCTGAAATAT GGAATTACCG AAGGTGTGCG CAATGTCACG CCGGACCAAT GGCTGGAGGA GGGGGACAAG GTTTGCGTTG CCGGGCATGT CTTCGACGTT TTCTATTGTC CCGGTCATGC GCCGGGCCAT GTCGTCTTTT TCAATCCTGA GGCGCGGTTT GCCATTGTCG GCGACGTGCT TTTCAACGGG TCGGTCGGCC GCACGGATCT TCCGGGGGGT GATCACGCGA CACTGATCCG CTCCATCAAG GAAAAGCTGC TGCCGCTTGG TGACGATATC GGCTTCATCT GCGGGCATGG TCCCGGCGGA CGGTTCGGGG AGGAACGGCG TTCCAATCCG TTTCTGACCG ATACGGGATA TGCC
Details for BrabA.10520.a.A1.GE27782
HARVESTED ON: 11/16/2009
SEQUENCED ON: 11/23/2009
EXPECTED MW: 25kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGQLQAIIV PVTPFQQNCT ILFDGETKKG VVIDPGGDLA RIREAIESQG IDVEAIWITH GHIDHAAAAL DLKEALGVDI IGPHRDDKFL LDNLVAAGLK YGITEGVRNV TPDQWLEEGD KVCVAGHVFD VFYCPGHAPG HVVFFNPEAR FAIVGDVLFN GSVGRTDLPG GDHATLIRSI KEKLLPLGDD IGFICGHGPG GRFGEERRSN PFLTNTG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggggc aattgcaggc cattatcgtt cccgtcacgc cgtttcagca aaattgcacc attctctttg atggtgaaac caagaagggg gtggtgatcg accccggcgg cgaccttgcg cgcatcaggg aagccattga atcccagggc atcgacgtcg aagcgatctg gatcacgcat ggccatatcg atcatgccgc tgccgcgctc gatctcaagg aggcgctggg tgtggatatt atcgggccgc acagggacga caagtttctc ctcgataatc tcgtggcggc tggcctgaaa tatggaatta ccgaaggtgt gcgcaatgtc acgccggacc aatggctgga ggagggggac aaggtttgcg ttgccgggca tgtcttcgac gttttctatt gtcccggtca tgcgccgggc catgtcgtct ttttcaatcc tgaggcgcgg tttgccattg tcggcgacgt gcttttcaac gggtcggtcg gccgcacgga tcttccgggg ggtgatcacg cgacactgat ccgctccatc aaggaaaagc tgctgccgct tggtgacgat atcggcttca tctgcgggca tggtcccggc ggacggttcg gggaggaacg gcgttccaat ccgtttctga ccaatacggg at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGQLQAIIV PVTPFQQNCT ILFDGETKKG VVIDPGGDLA RIREAIESQG IDVEAIWITH GHIDHAAAAL DLKEALGVDI IGPHRDDKFL LDNLVAAGLK YGITEGVRNV TPDQWLEEGD KVCVAGHVFD VFYCPGHAPG HVVFFNPEAR FAIVGDVLFN GSVGRTDLPG GDHATLIRSI KEKLLPLGDD IGFICGHGPG GRFGEERRSN PFLTDTGYA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggggcaattg caggccatta tcgttcccgt cacgccgttt cagcaaaatt gcaccattct ctttgatggt gaaaccaaga agggggtggt gatcgacccc ggcggcgacc ttgcgcgcat cagggaagcc attgaatccc agggcatcga cgtcgaagcg atctggatca cgcatggcca tatcgatcat gccgctgccg cgctcgatct caaggaggcg ctgggtgtgg atattatcgg gccgcacagg gacgacaagt ttctcctcga taatctcgtg gcggctggcc tgaaatatgg aattaccgaa ggtgtgcgca atgtcacgcc ggaccaatgg ctggaggagg gggacaaggt ttgcgttgcc gggcatgtct tcgacgtttt ctattgtccc ggtcatgcgc cgggccatgt cgtctttttc aatcctgagg cgcggtttgc cattgtcggc gacgtgcttt tcaacgggtc ggtcggccgc acggatcttc cggggggtga tcacgcgaca ctgatccgct ccatcaagga aaagctgctg ccgcttggtg acgatatcgg cttcatctgc gggcatggtc ccggcggacg gttcggggag gaacggcgtt ccaatccgtt tctgaccgat acgggatatg ccaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for BrabA.10520.a.A1.PW27670
PURIFICATION DATe: 12/28/2009
CONCENTRATION: 29.53mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGQLQAIIV PVTPFQQNCT ILFDGETKKG VVIDPGGDLA RIREAIESQG IDVEAIWITH GHIDHAAAAL DLKEALGVDI IGPHRDDKFL LDNLVAAGLK YGITEGVRNV TPDQWLEEGD KVCVAGHVFD VFYCPGHAPG HVVFFNPEAR FAIVGDVLFN GSVGRTDLPG GDHATLIRSI KEKLLPLGDD IGFICGHGPG GRFGEERRSN PFLTNTG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggggc aattgcaggc cattatcgtt cccgtcacgc cgtttcagca aaattgcacc attctctttg atggtgaaac caagaagggg gtggtgatcg accccggcgg cgaccttgcg cgcatcaggg aagccattga atcccagggc atcgacgtcg aagcgatctg gatcacgcat ggccatatcg atcatgccgc tgccgcgctc gatctcaagg aggcgctggg tgtggatatt atcgggccgc acagggacga caagtttctc ctcgataatc tcgtggcggc tggcctgaaa tatggaatta ccgaaggtgt gcgcaatgtc acgccggacc aatggctgga ggagggggac aaggtttgcg ttgccgggca tgtcttcgac gttttctatt gtcccggtca tgcgccgggc catgtcgtct ttttcaatcc tgaggcgcgg tttgccattg tcggcgacgt gcttttcaac gggtcggtcg gccgcacgga tcttccgggg ggtgatcacg cgacactgat ccgctccatc aaggaaaagc tgctgccgct tggtgacgat atcggcttca tctgcgggca tggtcccggc ggacggttcg gggaggaacg gcgttccaat ccgtttctga ccaatacggg at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGQLQAIIV PVTPFQQNCT ILFDGETKKG VVIDPGGDLA RIREAIESQG IDVEAIWITH GHIDHAAAAL DLKEALGVDI IGPHRDDKFL LDNLVAAGLK YGITEGVRNV TPDQWLEEGD KVCVAGHVFD VFYCPGHAPG HVVFFNPEAR FAIVGDVLFN GSVGRTDLPG GDHATLIRSI KEKLLPLGDD IGFICGHGPG GRFGEERRSN PFLTDTGYA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGGCAATTG CAGGCCATTA TCGTTCCCGT CACGCCGTTT CAGCAAAATT GCACCATTCT CTTTGATGGT GAAACCAAGA AGGGGGTGGT GATCGACCCC GGCGGCGACC TTGCGCGCAT CAGGGAAGCC ATTGAATCCC AGGGCATCGA CGTCGAAGCG ATCTGGATCA CGCATGGCCA TATCGATCAT GCCGCTGCCG CGCTCGATCT CAAGGAGGCG CTGGGTGTGG ATATTATCGG GCCGCACAGG GACGACAAGT TTCTCCTCGA TAATCTCGTG GCGGCTGGCC TGAAATATGG AATTACCGAA GGTGTGCGCA ATGTCACGCC GGACCAATGG CTGGAGGAGG GGGACAAGGT TTGCGTTGCC GGGCATGTCT TCGACGTTTT CTATTGTCCC GGTCATGCGC CGGGCCATGT CGTCTTTTTC AATCCTGAGG CGCGGTTTGC CATTGTCGGC GACGTGCTTT TCAACGGGTC GGTCGGCCGC ACGGATCTTC CGGGGGGTGA TCACGCGACA CTGATCCGCT CCATCAAGGA AAAGCTGCTG CCGCTTGGTG ACGATATCGG CTTCATCTGC GGGCATGGTC CCGGCGGACG GTTCGGGGAG GAACGGCGTT CCAATCCGTT TCTGACCGAT ACGGGATATG CCAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA