BrabA.10478.a
aminotransferase
| CENTER ID: | BrabA.10478.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.10478.a.A1.GE27781 | Full length( BrabA.10478.a ) | 1 | 290 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.10478.a.A1.PW27669 | Full length( BrabA.10478.a ) | 1 | 290 |
Structures
4TVI
DEPOSITED: 6/27/2014
DETERMINATION: XRay
CLONE: BrabA.10478.a.A1.GE27781
PROTEIN: BrabA.10478.a.A1.PW27669
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|359391.4.peg.2868 |
| RefSeq: | YP_418758.2 |
| UniProt: | Q2YKS6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQGTREMTDT RAMWTYYKGE WREGDVRILG AASQATWLGS LVFDGARLFE GVTPDLDRHS ARANDSARAL GLEPTLSAND IEALAREGLK KFAPDTDVYI RPMYWAEEGD ASTVAPLASS TDFALCLEAI PMVEPKGFTI TTTSFRRPYL EVMPVNAKAA CLYPNNARML REAKAKGFHN ALVTDVLGNV AETATSNVFM VRGGEVFTPV PNGTFLNGIT RQRVIKLLRE AGVSVHETTL KIEDFREADE IFSTGNMSKV VPIIGFDERK LDYGLVTKRA RALYWEWAHA
NT Sequence
TTGCAAGGAA CGAGAGAAAT GACCGACACA CGGGCAATGT GGACCTATTA CAAGGGCGAA TGGCGTGAAG GCGACGTACG CATTCTCGGC GCAGCCTCAC AGGCGACCTG GCTCGGCTCT CTCGTATTCG ACGGCGCACG GCTTTTTGAG GGTGTGACGC CCGATCTCGA TCGCCATTCG GCGCGCGCCA ACGATTCCGC TCGTGCGCTC GGCCTTGAGC CGACGCTTTC GGCCAATGAT ATCGAGGCAC TGGCGCGCGA GGGCCTGAAA AAATTTGCAC CGGACACGGA TGTCTATATC CGCCCCATGT ACTGGGCCGA AGAAGGCGAT GCCAGCACCG TTGCGCCGCT TGCCTCCTCC ACCGATTTTG CGCTCTGCCT TGAAGCCATT CCAATGGTAG AGCCGAAGGG TTTCACCATT ACCACCACTT CTTTCCGCCG CCCTTATCTT GAAGTCATGC CGGTCAACGC CAAGGCCGCC TGCCTCTATC CGAACAATGC CCGCATGTTG CGCGAAGCCA AGGCCAAGGG TTTTCATAAT GCGCTCGTCA CCGATGTTCT CGGCAATGTC GCCGAAACGG CGACGTCGAA CGTCTTCATG GTGCGCGGCG GCGAAGTCTT CACGCCGGTT CCAAATGGCA CATTCCTCAA CGGCATCACA CGCCAGCGCG TTATCAAGCT TTTGCGTGAA GCGGGGGTAA GTGTTCACGA AACGACGCTC AAGATCGAAG ATTTCCGCGA AGCGGACGAG ATTTTCTCCA CCGGCAACAT GAGCAAGGTC GTGCCGATCA TCGGTTTCGA CGAGCGCAAA CTCGATTATG GCCTGGTGAC AAAGCGTGCC CGCGCCCTTT ACTGGGAATG GGCCCACGCC
Details for BrabA.10478.a.A1.GE27781
| HARVESTED ON: | 11/16/2009 |
| SEQUENCED ON: | 11/23/2009 |
| EXPECTED MW: | 34kDa |
| OBSERVED MW: | 35kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLQGTREMT DTRAMWTYYK GEWREGDVRI LGAASQATWL GSLVFDGARL FEGVTPDLDR HSARANDSAR ALGLEPTLSA NDIEALAREG LKKFAPDTDV YIRPMYWAEE GDASTVAPLA SSTDFALCLE AIPMVEPKGF TITTTSFRRP YLEVMPVNAK AACLYPNNAR MLREAKAKGF HNALVTDVLG NVAETATSNV FMVRGGEVFT PVPNGTFLNG ITRQRVIKLL REAGVSVHET TLKIEDFREA DEIFSTGNMS KVVPIIGFDE RKLDYGLVTK RARALYWEW
Validated NT Sequence
gcccattccc agtaaagggc gcgggcacgc tttgtcacca ggccataatc gagtttgcgc tcgtcgaaac cgatgatcgg cacgaccttg ctcatgttgc cggtggagaa aatctcgtcc gcttcgcgga aatcttcgat cttgagcgtc gtttcgtgaa cacttacccc cgcttcacgc aaaagcttga taacgcgctg gcgtgtgatg ccgttgagga atgtgccatt tggaaccggc gtgaagactt cgccgccgcg caccatgaag acgttcgacg tcgccgtttc ggcgacattg ccgagaacat cggtgacgag cgcattatga aaacccttgg ccttggcttc gcgcaacatg cgggcattgt tcggatagag gcaggcggcc ttggcgttga ccggcatgac ttcaagataa gggcggcgga aagaagtggt ggtaatggtg aaacccttcg gctctaccat tggaatggct tcaaggcaga gcgcaaaatc ggtggaggag gcaagcggcg caacggtgct ggcatcgcct tcttcggccc agtacatggg gcggatatag acatccgtgt ccggtgcaaa ttttttcagg ccctcgcgcg ccagtgcctc gatatcattg gccgaaagcg tcggctcaag gccgagcgca cgagcggaat cgttggcgcg cgccgaatgg cgatcgagat cgggcgtcac accctcaaaa agccgtgcgc cgtcgaatac gagagagccg agccaggtcg cctgtgaggc tgcgccgaga atgcgtacgt cgccttcacg ccattcgccc ttgtaatagg tccacattgc ccgtgtgtcg gtcatttctc tcgttccttg caacatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLQGTREMT DTRAMWTYYK GEWREGDVRI LGAASQATWL GSLVFDGARL FEGVTPDLDR HSARANDSAR ALGLEPTLSA NDIEALAREG LKKFAPDTDV YIRPMYWAEE GDASTVAPLA SSTDFALCLE AIPMVEPKGF TITTTSFRRP YLEVMPVNAK AACLYPNNAR MLREAKAKGF HNALVTDVLG NVAETATSNV FMVRGGEVFT PVPNGTFLNG ITRQRVIKLL REAGVSVHET TLKIEDFREA DEIFSTGNMS KVVPIIGFDE RKLDYGLVTK RARALYWEWA HA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgcaagga acgagagaaa tgaccgacac acgggcaatg tggacctatt acaagggcga atggcgtgaa ggcgacgtac gcattctcgg cgcagcctca caggcgacct ggctcggctc tctcgtattc gacggcgcac ggctttttga gggtgtgacg cccgatctcg atcgccattc ggcgcgcgcc aacgattccg ctcgtgcgct cggccttgag ccgacgcttt cggccaatga tatcgaggca ctggcgcgcg agggcctgaa aaaatttgca ccggacacgg atgtctatat ccgccccatg tactgggccg aagaaggcga tgccagcacc gttgcgccgc ttgcctcctc caccgatttt gcgctctgcc ttgaagccat tccaatggta gagccgaagg gtttcaccat taccaccact tctttccgcc gcccttatct tgaagtcatg ccggtcaacg ccaaggccgc ctgcctctat ccgaacaatg cccgcatgtt gcgcgaagcc aaggccaagg gttttcataa tgcgctcgtc accgatgttc tcggcaatgt cgccgaaacg gcgacgtcga acgtcttcat ggtgcgcggc ggcgaagtct tcacgccggt tccaaatggc acattcctca acggcatcac acgccagcgc gttatcaagc ttttgcgtga agcgggggta agtgttcacg aaacgacgct caagatcgaa gatttccgcg aagcggacga gattttctcc accggcaaca tgagcaaggt cgtgccgatc atcggtttcg acgagcgcaa actcgattat ggcctggtga caaagcgtgc ccgcgccctt tactgggaat gggcccacgc caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BrabA.10478.a.A1.PW27669
| PURIFICATION DATe: | 3/15/2010 |
| CONCENTRATION: | 28.65mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLQGTREMT DTRAMWTYYK GEWREGDVRI LGAASQATWL GSLVFDGARL FEGVTPDLDR HSARANDSAR ALGLEPTLSA NDIEALAREG LKKFAPDTDV YIRPMYWAEE GDASTVAPLA SSTDFALCLE AIPMVEPKGF TITTTSFRRP YLEVMPVNAK AACLYPNNAR MLREAKAKGF HNALVTDVLG NVAETATSNV FMVRGGEVFT PVPNGTFLNG ITRQRVIKLL REAGVSVHET TLKIEDFREA DEIFSTGNMS KVVPIIGFDE RKLDYGLVTK RARALYWEW
Validated NT Sequence
gcccattccc agtaaagggc gcgggcacgc tttgtcacca ggccataatc gagtttgcgc tcgtcgaaac cgatgatcgg cacgaccttg ctcatgttgc cggtggagaa aatctcgtcc gcttcgcgga aatcttcgat cttgagcgtc gtttcgtgaa cacttacccc cgcttcacgc aaaagcttga taacgcgctg gcgtgtgatg ccgttgagga atgtgccatt tggaaccggc gtgaagactt cgccgccgcg caccatgaag acgttcgacg tcgccgtttc ggcgacattg ccgagaacat cggtgacgag cgcattatga aaacccttgg ccttggcttc gcgcaacatg cgggcattgt tcggatagag gcaggcggcc ttggcgttga ccggcatgac ttcaagataa gggcggcgga aagaagtggt ggtaatggtg aaacccttcg gctctaccat tggaatggct tcaaggcaga gcgcaaaatc ggtggaggag gcaagcggcg caacggtgct ggcatcgcct tcttcggccc agtacatggg gcggatatag acatccgtgt ccggtgcaaa ttttttcagg ccctcgcgcg ccagtgcctc gatatcattg gccgaaagcg tcggctcaag gccgagcgca cgagcggaat cgttggcgcg cgccgaatgg cgatcgagat cgggcgtcac accctcaaaa agccgtgcgc cgtcgaatac gagagagccg agccaggtcg cctgtgaggc tgcgccgaga atgcgtacgt cgccttcacg ccattcgccc ttgtaatagg tccacattgc ccgtgtgtcg gtcatttctc tcgttccttg caacatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLQGTREMT DTRAMWTYYK GEWREGDVRI LGAASQATWL GSLVFDGARL FEGVTPDLDR HSARANDSAR ALGLEPTLSA NDIEALAREG LKKFAPDTDV YIRPMYWAEE GDASTVAPLA SSTDFALCLE AIPMVEPKGF TITTTSFRRP YLEVMPVNAK AACLYPNNAR MLREAKAKGF HNALVTDVLG NVAETATSNV FMVRGGEVFT PVPNGTFLNG ITRQRVIKLL REAGVSVHET TLKIEDFREA DEIFSTGNMS KVVPIIGFDE RKLDYGLVTK RARALYWEWA HA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGCAAGGA ACGAGAGAAA TGACCGACAC ACGGGCAATG TGGACCTATT ACAAGGGCGA ATGGCGTGAA GGCGACGTAC GCATTCTCGG CGCAGCCTCA CAGGCGACCT GGCTCGGCTC TCTCGTATTC GACGGCGCAC GGCTTTTTGA GGGTGTGACG CCCGATCTCG ATCGCCATTC GGCGCGCGCC AACGATTCCG CTCGTGCGCT CGGCCTTGAG CCGACGCTTT CGGCCAATGA TATCGAGGCA CTGGCGCGCG AGGGCCTGAA AAAATTTGCA CCGGACACGG ATGTCTATAT CCGCCCCATG TACTGGGCCG AAGAAGGCGA TGCCAGCACC GTTGCGCCGC TTGCCTCCTC CACCGATTTT GCGCTCTGCC TTGAAGCCAT TCCAATGGTA GAGCCGAAGG GTTTCACCAT TACCACCACT TCTTTCCGCC GCCCTTATCT TGAAGTCATG CCGGTCAACG CCAAGGCCGC CTGCCTCTAT CCGAACAATG CCCGCATGTT GCGCGAAGCC AAGGCCAAGG GTTTTCATAA TGCGCTCGTC ACCGATGTTC TCGGCAATGT CGCCGAAACG GCGACGTCGA ACGTCTTCAT GGTGCGCGGC GGCGAAGTCT TCACGCCGGT TCCAAATGGC ACATTCCTCA ACGGCATCAC ACGCCAGCGC GTTATCAAGC TTTTGCGTGA AGCGGGGGTA AGTGTTCACG AAACGACGCT CAAGATCGAA GATTTCCGCG AAGCGGACGA GATTTTCTCC ACCGGCAACA TGAGCAAGGT CGTGCCGATC ATCGGTTTCG ACGAGCGCAA ACTCGATTAT GGCCTGGTGA CAAAGCGTGC CCGCGCCCTT TACTGGGAAT GGGCCCACGC CAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA