BrabA.10322.a

ATP/GTP-binding site motif A (P-loop):Cell divisionFtsK/SpoIIIE protein:AAA ATPase

CENTER ID: BrabA.10322.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.10322.a.A1.GE27780 Full length( BrabA.10322.a ) 1 531
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.1973
RefSeq: YP_415237.1
UniProt: Q2YLQ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEQTAPSPKP GPRAQREAQP SFLKDNGIFE MPSLHFLAEP KLVQRDPALS KDALEQNARL LEGVLEDFGV RGEIINVKPG PVVTLYELEP APGIKSSRVI GLADDIARSM SAIAARVAVI PGRNAIGIEL PNPKREMVYL REMLASRDFE QSKAKLALAL GKTINGEPVI ADIAKMPHVL VAGTTGSGKS VAINTMILSL LYRMTPQEFR LIMIDPKMLE LSVYDGIPHL LTPVVTDPKK AVVALKWTVR EMEDRYRKMS KVGVRNIDGF NQRVGLAQKK GEPIARTVQT GFDRNTGEAI YETEELDLEP MPYIVVIIDE MADLMMVAGK DIEGAVQRLA QMARAAGIHV IMATQRPSVD VITGTIKANF PTRISFQVTS KIDSRTILGE QGAEQLLGQG DMLFMAGGGR IQRVHGPFVG DDEVERIVQH LKLQGVPEYL DAITEDEDDD EGGSGPAGTG NLEDSDDPYD QAVAVVLRDK KASTSYIQRR LGIGYNRAAS IIERMEDEGI VGPANHAGKR EILVPTGDDD F
NT Sequence
GTGGAACAGA CCGCGCCGTC GCCGAAGCCC GGCCCGCGCG CCCAGCGCGA GGCGCAGCCT TCCTTCCTTA AGGATAATGG TATCTTTGAA ATGCCGTCGC TACATTTCCT GGCCGAACCG AAGCTCGTGC AGCGCGACCC CGCGCTTTCC AAGGATGCGC TGGAGCAGAA TGCCCGTCTG CTTGAGGGCG TGCTGGAAGA TTTCGGTGTG CGTGGCGAGA TCATCAATGT CAAACCCGGC CCCGTGGTCA CGCTTTATGA GCTTGAACCG GCACCGGGCA TCAAGTCCTC GCGCGTCATC GGCCTTGCCG ACGATATTGC GCGCTCGATG AGCGCGATTG CCGCGCGTGT GGCTGTCATT CCGGGGCGCA ACGCGATCGG TATCGAACTG CCGAACCCTA AGCGCGAAAT GGTCTATCTG CGTGAGATGC TGGCGAGCCG CGACTTTGAG CAATCGAAGG CGAAGCTTGC GCTGGCACTC GGCAAGACCA TCAATGGCGA GCCGGTCATT GCCGATATTG CCAAGATGCC GCACGTTCTG GTTGCGGGCA CCACCGGCTC GGGCAAGTCG GTTGCGATCA ACACCATGAT CCTGTCGTTG CTCTATCGCA TGACGCCGCA GGAATTCCGC CTCATCATGA TCGACCCCAA GATGCTGGAG CTTTCCGTCT ATGACGGCAT TCCGCATCTG CTGACGCCGG TTGTGACGGA CCCGAAGAAG GCCGTTGTCG CGCTCAAATG GACCGTGCGC GAAATGGAAG ACCGCTATCG CAAGATGTCG AAGGTCGGCG TGCGCAATAT TGACGGCTTC AACCAGCGTG TCGGTCTGGC GCAGAAAAAG GGCGAGCCGA TTGCGCGCAC GGTACAGACC GGCTTCGACC GCAATACCGG CGAGGCGATC TACGAGACGG AAGAACTCGA TCTCGAACCC ATGCCCTATA TCGTCGTGAT TATCGACGAA ATGGCCGACC TCATGATGGT CGCAGGCAAG GATATTGAAG GCGCGGTGCA GCGTCTGGCG CAGATGGCGC GTGCGGCAGG CATTCACGTC ATCATGGCGA CGCAGCGCCC GTCGGTCGAT GTCATCACCG GCACGATCAA GGCCAATTTC CCGACCCGCA TTTCCTTCCA GGTGACGTCG AAGATCGACA GCCGAACCAT TCTGGGCGAA CAGGGTGCCG AACAGCTTCT TGGCCAGGGC GACATGCTGT TCATGGCGGG CGGCGGGCGC ATCCAGCGCG TGCATGGGCC ATTTGTCGGC GACGACGAGG TGGAGCGCAT CGTGCAGCAT CTCAAGCTTC AGGGCGTGCC GGAATATCTC GATGCCATCA CCGAGGATGA AGACGATGAC GAGGGCGGTA GCGGCCCGGC TGGAACCGGC AATCTGGAGG ATTCCGACGA TCCGTATGAT CAGGCCGTGG CCGTCGTGCT GCGCGACAAG AAGGCGTCCA CATCCTATAT TCAGCGCCGT CTCGGCATCG GCTATAATCG CGCTGCCTCA ATCATCGAAC GCATGGAAGA TGAGGGCATT GTCGGCCCGG CCAACCATGC GGGCAAGCGC GAAATACTGG TGCCCACGGG CGATGATGAT TTC
Details for BrabA.10322.a.A1.GE27780
HARVESTED ON: 11/16/2009
SEQUENCED ON: 11/23/2009
EXPECTED MW: 60kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 98
Validated AA Sequence
MGTLEAQTQG PGSMVEQTAP SPKPGPRAQR EAQPSFLKDN GIFEMPSLHF LAEPKLVQRD PALSKDALEQ NARLLEGVLE DFGVRGEIIN VKPGPVVTLY ELEPAPGIKS SRVIGLADDI ARSMSAIAAR VAVIPGRNAI GIELPNPKRE MVYLREMLAS RDFEQSKAKL ALALGKTING EPVIADIAKM PHVLVAGTTG SGKSVAINXM ILSLLYRMTP XXXRLIMIDP XXXXLSVYDG IPHLLTPVVT DPKXAXVALK WXVREMEDRY RKMSKVGVRN XDGFNQRVXL AQKKXEPIAR TVQTGFDRNT GEAIYETEEL DLEPMPYIVV IIDEMADLMM VAGKDIEGAV QRLAQMARAA GIHVIMATQR PSVDVITGTI KANFPTRISF QVTSKIDSRT ILGEQGAEQL LGQGDMLFMA GGGRIQRVHG PFVGDDEVER IVQHLKLQGV PEYLDAITED EDDDEGGSGP AGTGNLEDSD DPYDQAVAVV LRDKKASTSY IQRRLGIGYN RAASIIERME DEGIVGPANH AGKREILVPT GDD
Validated NT Sequence
catcatcgcc cgtgggcacc agtatttcgc gcttgcccgc atggttggcc gggccgacaa tgccctcatc ttccatgcgt tcgatgattg aggcagcgcg attatagccg atgccgagac ggcgctgaat ataggatgtg gacgccttct tgtcgcgcag cacgacggcc acggcctgat catacggatc gtcggaatcc tccagattgc cggttccagc cgggccgcta ccgccctcgt catcgtcttc atcctcggtg atggcatcga gatattccgg cacgccctga agcttgagat gctgcacgat gcgctccacc tcgtcgtcgc cgacaaatgg cccatgcacg cgctggatgc gcccgccgcc cgccatgaac agcatgtcgc cctggccaag aagctgttcg gcaccctgtt cgcccagaat ggttcggctg tcgatcttcg acgtcacctg gaaggaaatg cgggtcggga aattggcctt gatcgtgccg gtgatgacat cgaccgacgg gcgctgcgtc gccatgatga cgtgaatgcc tgccgcacgc gccatctgcg ccagacgctg caccgcgcct tcaatatcct tgcctgcgac catcatgagg tcggccattt cgtcgataat cacgacgata tagggcatgg gttcgagatc gagttcttcc gtctcgtaga tcgcctcgcc ggtattgcgg tcgaagccgg tctgtaccgt gcgcgcaatc ggctcgncct ttttctgcgc cagancgaca cgctggttga agccgtcnat attgcgcacg ccgaccttcg acatcttgcg atagcggtct tccatttcgc gnacggncca tttgagcgcg acancngcnt tcttcgggtc cgtcacaacc ggcgtcagca gatgcggaat gccgtcatag acggaaagnn ccancntcnn ggggtcgatc atgatgaggc gganntcntg cggcgtcatg cgatagagca acgacaggat catgntgttg atcgcaaccg acttgcccga gccggtggtg cccgcaacca gaacgtgcgg catcttggca atatcggcaa tgaccggctc gccattgatg gtcttgccga gtgccagcgc aagcttcgcc ttcgattgct caaagtcgcg gctcgccagc atctcacgca gatagaccat ttcgcgctta gggttcggca gttcgatacc gatcgcgttg cgccccggaa tgacagccac acgcgcggca atcgcgctca tcgagcgcgc aatatcgtcg gcaaggccga tgacgcgcga ggacttgatg cccggtgccg gttcaagctc ataaagcgtg accacggggc cgggtttgac attgatgatc tcgccacgca caccgaaatc ttccagcacg ccctcaagca gacgggcatt ctgctccagc gcatccttgg aaagcgcggg gtcgcgctgc acgagcttcg gttcggccag gaaatgtagc gacggcattt caaagatacc attatcctta aggaaggaag gctgcgcctc gcgctgggcg cgcgggccgg gcttcggcga cggcgcggtc tgttccacca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatgg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVEQTAPSP KPGPRAQREA QPSFLKDNGI FEMPSLHFLA EPKLVQRDPA LSKDALEQNA RLLEGVLEDF GVRGEIINVK PGPVVTLYEL EPAPGIKSSR VIGLADDIAR SMSAIAARVA VIPGRNAIGI ELPNPKREMV YLREMLASRD FEQSKAKLAL ALGKTINGEP VIADIAKMPH VLVAGTTGSG KSVAINTMIL SLLYRMTPQE FRLIMIDPKM LELSVYDGIP HLLTPVVTDP KKAVVALKWT VREMEDRYRK MSKVGVRNID GFNQRVGLAQ KKGEPIARTV QTGFDRNTGE AIYETEELDL EPMPYIVVII DEMADLMMVA GKDIEGAVQR LAQMARAAGI HVIMATQRPS VDVITGTIKA NFPTRISFQV TSKIDSRTIL GEQGAEQLLG QGDMLFMAGG GRIQRVHGPF VGDDEVERIV QHLKLQGVPE YLDAITEDED DDEGGSGPAG TGNLEDSDDP YDQAVAVVLR DKKASTSYIQ RRLGIGYNRA ASIIERMEDE GIVGPANHAG KREILVPTGD DDF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggaacag accgcgccgt cgccgaagcc cggcccgcgc gcccagcgcg aggcgcagcc ttccttcctt aaggataatg gtatctttga aatgccgtcg ctacatttcc tggccgaacc gaagctcgtg cagcgcgacc ccgcgctttc caaggatgcg ctggagcaga atgcccgtct gcttgagggc gtgctggaag atttcggtgt gcgtggcgag atcatcaatg tcaaacccgg ccccgtggtc acgctttatg agcttgaacc ggcaccgggc atcaagtcct cgcgcgtcat cggccttgcc gacgatattg cgcgctcgat gagcgcgatt gccgcgcgtg tggctgtcat tccggggcgc aacgcgatcg gtatcgaact gccgaaccct aagcgcgaaa tggtctatct gcgtgagatg ctggcgagcc gcgactttga gcaatcgaag gcgaagcttg cgctggcact cggcaagacc atcaatggcg agccggtcat tgccgatatt gccaagatgc cgcacgttct ggttgcgggc accaccggct cgggcaagtc ggttgcgatc aacaccatga tcctgtcgtt gctctatcgc atgacgccgc aggaattccg cctcatcatg atcgacccca agatgctgga gctttccgtc tatgacggca ttccgcatct gctgacgccg gttgtgacgg acccgaagaa ggccgttgtc gcgctcaaat ggaccgtgcg cgaaatggaa gaccgctatc gcaagatgtc gaaggtcggc gtgcgcaata ttgacggctt caaccagcgt gtcggtctgg cgcagaaaaa gggcgagccg attgcgcgca cggtacagac cggcttcgac cgcaataccg gcgaggcgat ctacgagacg gaagaactcg atctcgaacc catgccctat atcgtcgtga ttatcgacga aatggccgac ctcatgatgg tcgcaggcaa ggatattgaa ggcgcggtgc agcgtctggc gcagatggcg cgtgcggcag gcattcacgt catcatggcg acgcagcgcc cgtcggtcga tgtcatcacc ggcacgatca aggccaattt cccgacccgc atttccttcc aggtgacgtc gaagatcgac agccgaacca ttctgggcga acagggtgcc gaacagcttc ttggccaggg cgacatgctg ttcatggcgg gcggcgggcg catccagcgc gtgcatgggc catttgtcgg cgacgacgag gtggagcgca tcgtgcagca tctcaagctt cagggcgtgc cggaatatct cgatgccatc accgaggatg aagacgatga cgagggcggt agcggcccgg ctggaaccgg caatctggag gattccgacg atccgtatga tcaggccgtg gccgtcgtgc tgcgcgacaa gaaggcgtcc acatcctata ttcagcgccg tctcggcatc ggctataatc gcgctgcctc aatcatcgaa cgcatggaag atgagggcat tgtcggcccg gccaaccatg cgggcaagcg cgaaatactg gtgcccacgg gcgatgatga tttcaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa