SSGCID: Target Page

BrabA.10201.a

methionyl-tRNA synthetase

CENTER ID:	BrabA.10201.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	in PDB
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.10201.a.A1.GE27779	Full length( BrabA.10201.a )		1	515

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.10201.a.A1.PW37326	Full length( BrabA.10201.a )		1	515

PROTEIN: BrabA.10201.a.A1.PW37326

Publications by SSGCID

Brucella melitensis methionyl-tRNA-synthetase (MetRS), a potential drug target for brucellosis

Barrett LK, Boyle SM, Choi R, Davies DR, Dranow DM, Edwards TE, Fan E, Lorimer D, Myers J, Nakazawa-Hewitt S, Ranade RM, Van Voorhis WC, Zhang Z, Ojo KK, Buckner F

PLoS ONE - 2016

volume 11, issue 8, pages e0160350

PMID: 27500735; PMCID: PMC4976878

PDB: 4DLP, 4PY2, 5K0S, 5K0T

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_127468
PATRIC ID:	fig\|359391.4.peg.1059
RefSeq:	YP_414420.1
UniProt:	Q2YQ76

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSREKYYITT AIAYPNGKPH IGHAYELIAT DAMARFQRLN GMDVYFLTGT DEHGIKMLQS ARKEGITPRD LADRNTSAFR RMAEVLNSSN DDYIRTSEER HYKASQAIWQ AMVANGDIYK GGYAGWYSVR DEAYYGEEET EVRADGVRYG PQGTPVEWVE EESYFFRLSA YQDKLLDLYE NNPGFIMPAE RRNEIVSFVK SGLKDLSISR TTFDWGIPVP GDEKHVMYVW VDALTNYITA LGYPDTTDER WAYWPANAHI IGKDISRFHA VYWPAFLMSA QLPLPKRVFA HGFLFNRGEK MSKSVGNVID PFELVERYGL DQLRYFLMRE VPFGQDGSYS HEAIVNRTNA DLANDLGNLA QRSLSMIAKN CEGKVPQPGA FSEADKAILD QADAALETAR KAMDDQALHL ALGAIFAVVA EANRYFAGQE PWALRKTDPA RMGTVLYVTA EVLRRVGIMV QPFIPQSAEK LLDILAVPAD KRQFADVLAS PLAGGTDLPA PQPVFPRYVE ADEQN

NT Sequence

ATGAGCCGCG AAAAATATTA CATCACGACT GCGATTGCTT ATCCGAACGG AAAGCCGCAT ATCGGCCACG CTTACGAACT GATCGCGACC GATGCCATGG CGCGGTTTCA GCGTCTCAAT GGCATGGATG TTTATTTTCT GACCGGCACG GACGAGCATG GCATCAAGAT GCTGCAAAGC GCGCGCAAGG AAGGCATTAC GCCGCGCGAT CTGGCAGACC GCAACACATC CGCCTTCCGT CGAATGGCTG AAGTGCTGAA CAGCTCCAAC GACGACTATA TCCGCACCTC CGAAGAGCGT CATTACAAGG CCAGCCAGGC CATTTGGCAG GCGATGGTCG CAAATGGGGA TATTTACAAG GGCGGCTATG CCGGCTGGTA TTCCGTGCGC GACGAAGCCT ATTACGGCGA GGAAGAAACG GAAGTGCGCG CGGATGGCGT CCGCTACGGC CCGCAGGGAA CGCCTGTGGA ATGGGTGGAG GAGGAAAGCT ATTTCTTCCG TCTTTCGGCC TATCAGGACA AGCTGCTCGA CCTCTACGAG AACAATCCGG GCTTTATCAT GCCCGCCGAA CGCCGCAATG AGATTGTGAG CTTCGTGAAG TCAGGTCTCA AGGACCTGTC GATTTCGCGC ACGACCTTCG ATTGGGGCAT TCCGGTGCCG GGAGATGAAA AGCACGTCAT GTATGTGTGG GTGGATGCGC TCACCAATTA CATCACTGCG CTCGGCTATC CTGATACGAC CGATGAGCGT TGGGCTTACT GGCCCGCCAA TGCCCATATC ATCGGCAAGG ATATTTCCCG CTTCCATGCG GTCTATTGGC CTGCATTCCT GATGTCGGCC CAATTACCCT TGCCGAAGCG TGTTTTCGCG CATGGGTTCC TGTTCAATCG CGGCGAGAAA ATGTCGAAGT CAGTCGGCAA TGTTATCGAT CCGTTTGAAC TGGTGGAGCG TTACGGCCTG GACCAGTTGC GTTATTTCCT GATGCGTGAA GTGCCATTCG GCCAGGATGG CAGCTACAGC CATGAGGCGA TCGTCAACCG CACCAATGCC GATCTGGCCA ACGACCTCGG CAATCTTGCC CAGCGTTCGC TATCGATGAT CGCCAAGAAT TGCGAAGGCA AGGTGCCGCA GCCCGGCGCA TTTTCCGAGG CTGACAAGGC CATTCTGGAT CAGGCCGATG CCGCGCTCGA AACCGCGCGC AAGGCAATGG ACGATCAGGC CCTGCATCTG GCATTGGGTG CGATCTTCGC TGTTGTGGCG GAAGCCAACC GCTATTTTGC GGGGCAGGAG CCATGGGCAC TGCGCAAGAC CGATCCTGCG CGCATGGGCA CCGTGCTCTA TGTAACAGCC GAGGTTCTGC GCCGCGTCGG CATCATGGTG CAGCCTTTCA TCCCGCAATC TGCGGAGAAG CTGCTCGATA TTCTGGCTGT CCCGGCTGAC AAGCGCCAGT TTGCCGATGT GCTGGCAAGC CCGCTTGCTG GTGGGACCGA TCTGCCTGCG CCGCAGCCGG TCTTCCCGCG CTATGTGGAA GCGGACGAAC AGAAC

Details for BrabA.10201.a.A1.GE27779

HARVESTED ON:	11/16/2009
SEQUENCED ON:	11/23/2009
EXPECTED MW:	60kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	97
PERCENT COVERAGE:	45

Validated AA Sequence

Validated NT Sequence

ttcgtccgct tccacatagc gcgggaagac cggctgcggc gcaggcagat cggtcccacc agcaagcggg cttgccagca catcggcaaa ctggcgcttg tcagccggga cagccagaat atcgagcagc ttctccgcag attgcgggat gaaaggctgc accatgatgc cgacgcggcg cagaacctcg gctgttacat agagcacggt gcccatgcgc gcaggatcgg tcttgcgcag tgcccatggc tcctgccccg caaaatagcg gttggcttcc gccacaacag cgaagatcgc acccaatgcc agatgcaggg cctgatcgtc cattgccttg cgcgcggttt cgagcgcggc atcggcctga tccagaatgg ccttgtcagc ctcggaaaat gcgccgggct gcggcacctt gccttcgcaa ttcttggcga tcatcgatag cgaacgctgg gcaagattgc cgaggtcgtt ggccagatcg gcattggtgc ggttgacgat cgcctcatgg ctgtagctgc catcctggcc gaatggcact tcacgcatca ggaaataacg caactggtcc aggccgtaac gctccaccag ttcaaacgga tcgataacat tgccgactga cttcgacatt ttctcgccgc gattgaacag gaacccatgc gcgaaaacac gcttcggcaa gggtaattgg gccgacatca ggaatgcagg ccaatagacc gcatggaagc gggaaatatc cttgccgatg atatgggcat tggcgggcca gtaagcccaa cgctcatcgg tcgtatcagg atagccgagc gcagtgatgt aattggtgag cgcatccacc cacacataca tgacgtgctt ttcatctccc ggcaccggaa tgcccccaat cgaaggtcgt gcgcgaaatc gacaggtcct tgagacctga cttcacgaag ctcacaatct cattgcggcg ttcggcgggc atgataaagc ccggattgtt ctcgtagagg tcgagcagct tgtcctgata ggccgaaaga cggaagaaat agctttcctc ctccacccat tccacaggcg ttccctgcgg gccgtagcgg acgccatccg cgcgcacttc cgtttcttcc tcgccgtaat aggcttcgtc gcgcacggaa taccagccgg catagccgcc cttgtaaata tccccatttg cgaccatcgc ctgccaaatg gcctggctgg ccttgtaatg acgctcttcg gaggtgcgga tatagtcgtc gttggagctg ttcagcactt cagccattcg acggaaggcg gatgtgttgc ggtctgccag atcgcgcggc gtaatgcctt ccttgcgcgc gctttgcagc atcttgatgc catgctcgtc cgtgccggtc agaaaataaa catccatgcc attgagacgc tgaaaccgcg ccatggcatc ggtcgcgatc agttcgtaag cgtggccgat atgcggcttt ccgttcggat aagcaatcgc agtcgtgatg taatattttt cgcggctcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSREKYYIT TAIAYPNGKP HIGHAYELIA TDAMARFQRL NGMDVYFLTG TDEHGIKMLQ SARKEGITPR DLADRNTSAF RRMAEVLNSS NDDYIRTSEE RHYKASQAIW QAMVANGDIY KGGYAGWYSV RDEAYYGEEE TEVRADGVRY GPQGTPVEWV EEESYFFRLS AYQDKLLDLY ENNPGFIMPA ERRNEIVSFV KSGLKDLSIS RTTFDWGIPV PGDEKHVMYV WVDALTNYIT ALGYPDTTDE RWAYWPANAH IIGKDISRFH AVYWPAFLMS AQLPLPKRVF AHGFLFNRGE KMSKSVGNVI DPFELVERYG LDQLRYFLMR EVPFGQDGSY SHEAIVNRTN ADLANDLGNL AQRSLSMIAK NCEGKVPQPG AFSEADKAIL DQADAALETA RKAMDDQALH LALGAIFAVV AEANRYFAGQ EPWALRKTDP ARMGTVLYVT AEVLRRVGIM VQPFIPQSAE KLLDILAVPA DKRQFADVLA SPLAGGTDLP APQPVFPRYV EADEQN

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagccgcgaa aaatattaca tcacgactgc gattgcttat ccgaacggaa agccgcatat cggccacgct tacgaactga tcgcgaccga tgccatggcg cggtttcagc gtctcaatgg catggatgtt tattttctga ccggcacgga cgagcatggc atcaagatgc tgcaaagcgc gcgcaaggaa ggcattacgc cgcgcgatct ggcagaccgc aacacatccg ccttccgtcg aatggctgaa gtgctgaaca gctccaacga cgactatatc cgcacctccg aagagcgtca ttacaaggcc agccaggcca tttggcaggc gatggtcgca aatggggata tttacaaggg cggctatgcc ggctggtatt ccgtgcgcga cgaagcctat tacggcgagg aagaaacgga agtgcgcgcg gatggcgtcc gctacggccc gcagggaacg cctgtggaat gggtggagga ggaaagctat ttcttccgtc tttcggccta tcaggacaag ctgctcgacc tctacgagaa caatccgggc tttatcatgc ccgccgaacg ccgcaatgag attgtgagct tcgtgaagtc aggtctcaag gacctgtcga tttcgcgcac gaccttcgat tggggcattc cggtgccggg agatgaaaag cacgtcatgt atgtgtgggt ggatgcgctc accaattaca tcactgcgct cggctatcct gatacgaccg atgagcgttg ggcttactgg cccgccaatg cccatatcat cggcaaggat atttcccgct tccatgcggt ctattggcct gcattcctga tgtcggccca attacccttg ccgaagcgtg ttttcgcgca tgggttcctg ttcaatcgcg gcgagaaaat gtcgaagtca gtcggcaatg ttatcgatcc gtttgaactg gtggagcgtt acggcctgga ccagttgcgt tatttcctga tgcgtgaagt gccattcggc caggatggca gctacagcca tgaggcgatc gtcaaccgca ccaatgccga tctggccaac gacctcggca atcttgccca gcgttcgcta tcgatgatcg ccaagaattg cgaaggcaag gtgccgcagc ccggcgcatt ttccgaggct gacaaggcca ttctggatca ggccgatgcc gcgctcgaaa ccgcgcgcaa ggcaatggac gatcaggccc tgcatctggc attgggtgcg atcttcgctg ttgtggcgga agccaaccgc tattttgcgg ggcaggagcc atgggcactg cgcaagaccg atcctgcgcg catgggcacc gtgctctatg taacagccga ggttctgcgc cgcgtcggca tcatggtgca gcctttcatc ccgcaatctg cggagaagct gctcgatatt ctggctgtcc cggctgacaa gcgccagttt gccgatgtgc tggcaagccc gcttgctggt gggaccgatc tgcctgcgcc gcagccggtc ttcccgcgct atgtggaagc ggacgaacag aacaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for BrabA.10201.a.A1.PW37326

PURIFICATION DATe:	1/22/2013
CONCENTRATION:	21.4mg/ml
OBSERVED MW:	63kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	11
VIAL VOLUME:	200µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	45

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCCGCGAA AAATATTACA TCACGACTGC GATTGCTTAT CCGAACGGAA AGCCGCATAT CGGCCACGCT TACGAACTGA TCGCGACCGA TGCCATGGCG CGGTTTCAGC GTCTCAATGG CATGGATGTT TATTTTCTGA CCGGCACGGA CGAGCATGGC ATCAAGATGC TGCAAAGCGC GCGCAAGGAA GGCATTACGC CGCGCGATCT GGCAGACCGC AACACATCCG CCTTCCGTCG AATGGCTGAA GTGCTGAACA GCTCCAACGA CGACTATATC CGCACCTCCG AAGAGCGTCA TTACAAGGCC AGCCAGGCCA TTTGGCAGGC GATGGTCGCA AATGGGGATA TTTACAAGGG CGGCTATGCC GGCTGGTATT CCGTGCGCGA CGAAGCCTAT TACGGCGAGG AAGAAACGGA AGTGCGCGCG GATGGCGTCC GCTACGGCCC GCAGGGAACG CCTGTGGAAT GGGTGGAGGA GGAAAGCTAT TTCTTCCGTC TTTCGGCCTA TCAGGACAAG CTGCTCGACC TCTACGAGAA CAATCCGGGC TTTATCATGC CCGCCGAACG CCGCAATGAG ATTGTGAGCT TCGTGAAGTC AGGTCTCAAG GACCTGTCGA TTTCGCGCAC GACCTTCGAT TGGGGCATTC CGGTGCCGGG AGATGAAAAG CACGTCATGT ATGTGTGGGT GGATGCGCTC ACCAATTACA TCACTGCGCT CGGCTATCCT GATACGACCG ATGAGCGTTG GGCTTACTGG CCCGCCAATG CCCATATCAT CGGCAAGGAT ATTTCCCGCT TCCATGCGGT CTATTGGCCT GCATTCCTGA TGTCGGCCCA ATTACCCTTG CCGAAGCGTG TTTTCGCGCA TGGGTTCCTG TTCAATCGCG GCGAGAAAAT GTCGAAGTCA GTCGGCAATG TTATCGATCC GTTTGAACTG GTGGAGCGTT ACGGCCTGGA CCAGTTGCGT TATTTCCTGA TGCGTGAAGT GCCATTCGGC CAGGATGGCA GCTACAGCCA TGAGGCGATC GTCAACCGCA CCAATGCCGA TCTGGCCAAC GACCTCGGCA ATCTTGCCCA GCGTTCGCTA TCGATGATCG CCAAGAATTG CGAAGGCAAG GTGCCGCAGC CCGGCGCATT TTCCGAGGCT GACAAGGCCA TTCTGGATCA GGCCGATGCC GCGCTCGAAA CCGCGCGCAA GGCAATGGAC GATCAGGCCC TGCATCTGGC ATTGGGTGCG ATCTTCGCTG TTGTGGCGGA AGCCAACCGC TATTTTGCGG GGCAGGAGCC ATGGGCACTG CGCAAGACCG ATCCTGCGCG CATGGGCACC GTGCTCTATG TAACAGCCGA GGTTCTGCGC CGCGTCGGCA TCATGGTGCA GCCTTTCATC CCGCAATCTG CGGAGAAGCT GCTCGATATT CTGGCTGTCC CGGCTGACAA GCGCCAGTTT GCCGATGTGC TGGCAAGCCC GCTTGCTGGT GGGACCGATC TGCCTGCGCC GCAGCCGGTC TTCCCGCGCT ATGTGGAAGC GGACGAACAG AACAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA