BrabA.10154.a

Histone deacetylase family

CENTER ID: BrabA.10154.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.10154.a.MB1.GE30435 Full length( BrabA.10154.a ) 1 337
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.477
RefSeq: YP_413916.1
UniProt: Q2YMF6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHFPGRRRFT YFLRKMLYSG CMTTRLYWHP IYLEHLTPPG HPERPDRIRA LMSELEGPDF YRLDRVEAPH AGEAAILLAH PEEHLEAVRS KIPEPVEDGE ASQPIVKLDG DTYVSPKSMD AALTAIGAAM AAVDDVMSGA ADNVFVASRP PGHHAERSRA MGFCVFNNIA IAARHAQRHH GLERIAIVDG DVHHGNGTQD IFKDDPGVMF CSTHQFPLYP GSGDKHETGV GNIVNAPLSP NTGSREFREA FNSRILPALD NFRPDLILIS AGFDAHFRDP LAEINLDESD FDWATGKLME RAERFCDHRL VSVLEGGYDL EGLSQSASVH ITRLLKG
NT Sequence
ATGCATTTTC CGGGCCGAAG GCGTTTCACA TATTTTTTGC GGAAAATGCT TTATAGTGGA TGCATGACGA CACGGCTTTA CTGGCATCCG ATCTATCTCG AACACCTGAC GCCGCCCGGC CACCCGGAAC GCCCCGACCG CATTCGCGCG TTGATGAGCG AGCTGGAGGG GCCGGATTTC TACCGCCTTG ACCGGGTGGA AGCGCCGCAT GCCGGTGAGG CGGCGATCCT GCTTGCCCAC CCGGAAGAAC ATCTGGAAGC GGTGCGGTCG AAAATTCCCG AACCTGTTGA GGACGGGGAA GCGTCCCAGC CCATCGTGAA ACTTGATGGT GATACCTATG TCAGCCCGAA AAGCATGGAT GCGGCGCTGA CAGCCATTGG CGCGGCGATG GCCGCGGTCG ATGATGTCAT GTCGGGTGCT GCGGACAATG TTTTCGTGGC ATCGCGCCCG CCTGGCCACC ATGCCGAGCG CAGCAGGGCC ATGGGCTTTT GCGTTTTCAA CAATATTGCG ATTGCGGCGC GCCATGCGCA GCGCCATCAT GGGCTGGAAC GCATCGCAAT TGTCGATGGG GATGTACACC ACGGCAATGG CACGCAGGAT ATTTTCAAGG ATGATCCAGG CGTAATGTTC TGTTCGACGC ACCAGTTCCC GCTCTATCCG GGAAGTGGTG ACAAGCACGA AACGGGCGTG GGCAATATCG TCAATGCGCC GCTTTCGCCC AATACGGGCA GTCGCGAGTT TCGTGAGGCG TTCAATAGTC GTATTTTACC GGCCCTGGAC AATTTCCGGC CAGATTTGAT CCTTATTTCG GCTGGCTTCG ATGCCCATTT TCGCGACCCT CTGGCCGAGA TCAATCTCGA TGAGTCGGAT TTCGATTGGG CGACGGGCAA GCTCATGGAG CGGGCAGAAA GGTTCTGCGA TCACCGACTG GTGAGTGTGC TTGAAGGTGG ATATGATCTA GAGGGCTTGT CGCAGTCCGC ATCTGTGCAT ATTACGCGGC TGTTGAAAGG A
Details for BrabA.10154.a.MB1.GE30435
HARVESTED ON: 5/11/2010
SEQUENCED ON: 10/7/2010
EXPECTED MW: 82kDa
OBSERVED MW: 82kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 75
PERCENT COVERAGE: 48
Validated AA Sequence
MHFPGRRRFT YFLRKMLYSG CMTTRLYWHP IYLEHLTPPG HPERPDRIRA LMSELEGPDF YRLDRVEAPH AGEAAILLAH PEEHLEAVRS KIPEPVEDGE ASQPIVKLDG DTYVSPKSMD AALTAIGAAM AAVDDVMSGA ADNVFVASRP PGHHAERSRA MGFCVFNNIA IAARHAQRHH GLERIAIVDG DVHHGNGTQX IFKDDPGVMF CSTHQFPLYP GSGDKHETGV GXIVNAPXXP IRAVASFVRR SIVVFYRPXX IXXPXXSXXG WLRCPFXDXX AEIXLDEXEX XXXGXXXXXX KXXXXXXXXX XXXXXLXGXX XXIXXXXXXX XXXXXXXXXX XXXXLXXXXX XXXXXXXXXX XXX
Validated NT Sequence
gngctcngac ccngggtcct ggttcgatgc attttccggg ccgaaggcgt ttcacatatt ttttgcggaa aatgctttat agtggatgca tgacgacacg gctttactgg catccgatct atctcgaaca cctgacgccg cccggccacc cggaacgccc cgaccgcatt cgcgcgttga tgagcgagct ggaggggccg gatttctacc gccttgaccg ggtggaagcg ccgcatgccg gtgaggcggc gatcctgctt gcccacccgg aagaacatct ggaagcggtg cggtcgaaaa ttcccgaacc tgttgaggac ggggaagcgt cccagcccat cgtgaaactt gatggtgata cctatgtcag cccgaaaagc atggatgcgg cgctgacagc cattggcgcg gcgatggccg cggtcgatga tgtcatgtcg ggtgctgcgg acaatgtttt cgtggcatcg cgcccgcctg gccaccatgc cgagcgcagc agggccatgg gcttttgcgt tttcaacaat attgcgattg cggcgcgcca tgcgcagcgc catcatgggc tggaacgcat cgcaattgtc gatggggatg tacaccacgg caatggcacg cagganattt tcaaggatga tccaggcgta atgttctgtt cgacgcacca gttcccgctc tatccgggaa gtggtgacaa gcacgaaacg ggcgtgggcn ntatcgtcaa tgcgccgcnn ngcccaatac gggcngtcgc gagtttcgtg aggcgttcna tagtcgtatt ttaccggccc tngncnattt ncnggccaga nngatccnna nnnggctggc ttcgatgccc atttnncgac cnnntggccg agatcnatct cgatgagncg gaantcgann nnnacgggnn agnncangnn nnnnanaaan nnngcnatcn cngacnggng ngnnnnnctn nnnnnnatna nctannnggn tnncncannn ncatctnnnn nnnnnnnnnn nnnnnannnn annnnnanga nantnngcnn nnnnnnnnnn nnnnnnngct annnanncnn nnnnnnnncn nnnnnnnnnn tnnnnnnnnn nnanannnnn nntnnc
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MHFPGRRRFT YFLRKMLYSG CMTTRLYWHP IYLEHLTPPG HPERPDRIRA LMSELEGPDF YRLDRVEAPH AGEAAILLAH PEEHLEAVRS KIPEPVEDGE ASQPIVKLDG DTYVSPKSMD AALTAIGAAM AAVDDVMSGA ADNVFVASRP PGHHAERSRA MGFCVFNNIA IAARHAQRHH GLERIAIVDG DVHHGNGTQD IFKDDPGVMF CSTHQFPLYP GSGDKHETGV GNIVNAPLSP NTGSREFREA FNSRILPALD NFRPDLILIS AGFDAHFRDP LAEINLDESD FDWATGKLME RAERFCDHRL VSVLEGGYDL EGLSQSASVH ITRLLKG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatgca ttttccgggc cgaaggcgtt tcacatattt tttgcggaaa atgctttata gtggatgcat gacgacacgg ctttactggc atccgatcta tctcgaacac ctgacgccgc ccggccaccc ggaacgcccc gaccgcattc gcgcgttgat gagcgagctg gaggggccgg atttctaccg ccttgaccgg gtggaagcgc cgcatgccgg tgaggcggcg atcctgcttg cccacccgga agaacatctg gaagcggtgc ggtcgaaaat tcccgaacct gttgaggacg gggaagcgtc ccagcccatc gtgaaacttg atggtgatac ctatgtcagc ccgaaaagca tggatgcggc gctgacagcc attggcgcgg cgatggccgc ggtcgatgat gtcatgtcgg gtgctgcgga caatgttttc gtggcatcgc gcccgcctgg ccaccatgcc gagcgcagca gggccatggg cttttgcgtt ttcaacaata ttgcgattgc ggcgcgccat gcgcagcgcc atcatgggct ggaacgcatc gcaattgtcg atggggatgt acaccacggc aatggcacgc aggatatttt caaggatgat ccaggcgtaa tgttctgttc gacgcaccag ttcccgctct atccgggaag tggtgacaag cacgaaacgg gcgtgggcaa tatcgtcaat gcgccgcttt cgcccaatac gggcagtcgc gagtttcgtg aggcgttcaa tagtcgtatt ttaccggccc tggacaattt ccggccagat ttgatcctta tttcggctgg cttcgatgcc cattttcgcg accctctggc cgagatcaat ctcgatgagt cggatttcga ttgggcgacg ggcaagctca tggagcgggc agaaaggttc tgcgatcacc gactggtgag tgtgcttgaa ggtggatatg atctagaggg cttgtcgcag tccgcatctg tgcatattac gcggctgttg aaaggataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa