BrabA.10100.c

Serine protease, V8 family:Serine protease, trypsin family:PDZ/DHR/GLGF domain:HtrA/DegQ protease:Glucose/ribitol dehydrogenase

CENTER ID: BrabA.10100.c
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.10100.c.A2.GE27777 Mature protein( BrabA.10100.c ) 37 524
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.1469
RefSeq: YP_414785.1
UniProt: Q2YS27

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAIAPKAGFA RTLFATVALG AMSVAGTVSM GTPPALAAQG PASVADLAEG LLDAVVNIST SQTVKDDGEG DGPVPMPQVP EGSPFQEFFK DFFNDKDGAQ GDDSRKVQSL GSGFIIDAEK GYIVTNNHVI ADADEIEVNF NDGSKLKAEL VGKDTKTDLA ILKVDPSKHK LKAVHFGNSE KARIGDWVLA IGNPFGLGGT VTAGIISARK RDINSGPYDD FIQTDAAINR GNSGGPLFDM DGKVIGINTA IISPSGGSIG IGFAIPAEMA AGVIDQLKEF GEVRRGWLGV RLQPVTEDIA QSLGLKETKG ALIAGLIENS GVDNKAIEAG DVVIRFDGKP VDTARDLPRL VAESPVGKEV EIVVIRQGAE KTLKVKLGCL VEDDKSTEPA VEDQVPAPDD GEQPGARQET PDKSDKPKKE HKSDAAPSVL GMKLSKLDDD IRGEFGIAED VEGVAILYVA PGTAAGEKRI ETGDVIVDIG QVTVKTPEDV KKRIDALRRE GRKNALLMLA SRSGELRFVT IRID
NT Sequence
ATGGCAATTG CACCAAAGGC GGGTTTTGCT CGCACCCTTT TCGCAACCGT TGCACTGGGG GCGATGAGTG TCGCGGGCAC TGTGTCGATG GGAACGCCGC CCGCGCTGGC TGCGCAAGGT CCCGCTTCGG TGGCCGATCT TGCCGAAGGG CTTCTGGATG CAGTCGTGAA TATCTCCACA TCGCAGACCG TGAAGGACGA TGGCGAGGGG GACGGCCCGG TGCCGATGCC GCAGGTGCCG GAAGGTTCGC CCTTCCAGGA ATTCTTCAAA GACTTCTTCA ATGACAAGGA TGGCGCGCAG GGCGATGATT CACGCAAGGT GCAGTCGCTG GGTTCGGGTT TCATCATCGA TGCCGAAAAG GGTTACATCG TCACCAACAA TCACGTCATC GCCGATGCCG ACGAGATTGA GGTGAATTTC AACGATGGTT CCAAGCTCAA GGCGGAGCTC GTTGGCAAGG ATACGAAGAC CGATCTTGCG ATCCTGAAAG TCGACCCTTC CAAGCATAAG CTCAAGGCGG TGCATTTCGG CAATTCGGAA AAGGCGCGCA TTGGCGATTG GGTTCTGGCG ATCGGCAATC CGTTCGGCCT TGGCGGTACT GTCACGGCAG GCATCATTTC AGCACGCAAG CGCGATATCA ATTCCGGCCC CTATGACGAT TTCATTCAGA CGGATGCCGC GATCAATCGC GGCAATTCCG GCGGGCCGCT GTTTGATATG GACGGCAAGG TGATCGGCAT CAATACGGCG ATCATTTCGC CTTCTGGCGG GTCGATCGGC ATTGGTTTTG CTATTCCGGC GGAAATGGCC GCCGGTGTCA TCGATCAGTT GAAGGAATTT GGCGAAGTGC GCCGCGGCTG GCTTGGCGTC CGTCTTCAGC CGGTGACGGA AGACATTGCC CAGAGCCTCG GGCTGAAAGA GACCAAGGGC GCGCTGATTG CCGGTCTCAT CGAAAATTCC GGTGTCGATA ACAAGGCGAT AGAGGCGGGC GATGTGGTGA TCCGCTTTGA CGGCAAACCG GTGGATACGG CGCGCGATCT TCCGCGCCTC GTGGCGGAAA GCCCGGTTGG CAAGGAAGTG GAAATCGTCG TTATCCGTCA GGGCGCGGAA AAGACCCTGA AGGTCAAGCT TGGCTGTCTG GTCGAGGACG ACAAGAGCAC GGAGCCGGCG GTTGAAGATC AGGTGCCCGC GCCGGATGAC GGCGAACAGC CGGGAGCCCG GCAGGAGACG CCGGACAAGT CGGACAAGCC GAAGAAGGAA CACAAGAGCG ATGCTGCTCC AAGCGTTCTC GGCATGAAGC TCAGCAAGCT CGATGATGAT ATTCGCGGCG AGTTTGGCAT TGCCGAAGAT GTGGAGGGCG TTGCCATCCT CTATGTTGCA CCGGGGACGG CTGCCGGCGA AAAGCGCATC GAAACGGGCG ATGTCATTGT CGATATCGGT CAGGTGACGG TGAAGACGCC GGAAGATGTG AAAAAGCGTA TCGATGCACT GCGCCGCGAG GGGCGCAAGA ATGCGCTTTT GATGCTGGCT TCGCGTTCCG GGGAGTTGCG TTTCGTGACG ATCCGTATCG AC
Details for BrabA.10100.c.A2.GE27777
HARVESTED ON: data unavailable
SEQUENCED ON: 11/23/2009
EXPECTED MW: 54kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 28
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAAQGPASV ADLAEGLLDA VVNISTSQTV KDDGEGDGPV PMPQVPEGSP FQEFFKDFFN DKDGGEKRIE TGDVIVDIGQ VTVKTPEDVK KRIDALRREG RKNALLMLAS RSGELRFVTI RID
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggctg cgcaaggtcc cgcttcggtg gccgatcttg ccgaagggct tctggatgca gtcgtgaata tctccacatc gcagaccgtg aaggacgatg gcgaggggga cggcccggtg ccgatgccgc aggtgccgga aggttcgccc ttccaggaat tcttcaaaga cttcttcaat gacaaggatg gcggcgaaaa gcgcatcgaa acgggcgatg tcattgtcga tatcggtcag gtgacggtga agacgccgga agatgtgaaa aagcgtatcg atgcactgcg ccgcgagggg cgcaagaatg cgcttttgat gctggcttcg cgttccgggg agttgcgttt cgtgacgatc cgtatcgact aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAQGPASV ADLAEGLLDA VVNISTSQTV KDDGEGDGPV PMPQVPEGSP FQEFFKDFFN DKDGAQGDDS RKVQSLGSGF IIDAEKGYIV TNNHVIADAD EIEVNFNDGS KLKAELVGKD TKTDLAILKV DPSKHKLKAV HFGNSEKARI GDWVLAIGNP FGLGGTVTAG IISARKRDIN SGPYDDFIQT DAAINRGNSG GPLFDMDGKV IGINTAIISP SGGSIGIGFA IPAEMAAGVI DQLKEFGEVR RGWLGVRLQP VTEDIAQSLG LKETKGALIA GLIENSGVDN KAIEAGDVVI RFDGKPVDTA RDLPRLVAES PVGKEVEIVV IRQGAEKTLK VKLGCLVEDD KSTEPAVEDQ VPAPDDGEQP GARQETPDKS DKPKKEHKSD AAPSVLGMKL SKLDDDIRGE FGIAEDVEGV AILYVAPGTA AGEKRIETGD VIVDIGQVTV KTPEDVKKRI DALRREGRKN ALLMLASRSG ELRFVTIRID
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgcgcaa ggtcccgctt cggtggccga tcttgccgaa gggcttctgg atgcagtcgt gaatatctcc acatcgcaga ccgtgaagga cgatggcgag ggggacggcc cggtgccgat gccgcaggtg ccggaaggtt cgcccttcca ggaattcttc aaagacttct tcaatgacaa ggatggcgcg cagggcgatg attcacgcaa ggtgcagtcg ctgggttcgg gtttcatcat cgatgccgaa aagggttaca tcgtcaccaa caatcacgtc atcgccgatg ccgacgagat tgaggtgaat ttcaacgatg gttccaagct caaggcggag ctcgttggca aggatacgaa gaccgatctt gcgatcctga aagtcgaccc ttccaagcat aagctcaagg cggtgcattt cggcaattcg gaaaaggcgc gcattggcga ttgggttctg gcgatcggca atccgttcgg ccttggcggt actgtcacgg caggcatcat ttcagcacgc aagcgcgata tcaattccgg cccctatgac gatttcattc agacggatgc cgcgatcaat cgcggcaatt ccggcgggcc gctgtttgat atggacggca aggtgatcgg catcaatacg gcgatcattt cgccttctgg cgggtcgatc ggcattggtt ttgctattcc ggcggaaatg gccgccggtg tcatcgatca gttgaaggaa tttggcgaag tgcgccgcgg ctggcttggc gtccgtcttc agccggtgac ggaagacatt gcccagagcc tcgggctgaa agagaccaag ggcgcgctga ttgccggtct catcgaaaat tccggtgtcg ataacaaggc gatagaggcg ggcgatgtgg tgatccgctt tgacggcaaa ccggtggata cggcgcgcga tcttccgcgc ctcgtggcgg aaagcccggt tggcaaggaa gtggaaatcg tcgttatccg tcagggcgcg gaaaagaccc tgaaggtcaa gcttggctgt ctggtcgagg acgacaagag cacggagccg gcggttgaag atcaggtgcc cgcgccggat gacggcgaac agccgggagc ccggcaggag acgccggaca agtcggacaa gccgaagaag gaacacaaga gcgatgctgc tccaagcgtt ctcggcatga agctcagcaa gctcgatgat gatattcgcg gcgagtttgg cattgccgaa gatgtggagg gcgttgccat cctctatgtt gcaccgggga cggctgccgg cgaaaagcgc atcgaaacgg gcgatgtcat tgtcgatatc ggtcaggtga cggtgaagac gccggaagat gtgaaaaagc gtatcgatgc actgcgccgc gaggggcgca agaatgcgct tttgatgctg gcttcgcgtt ccggggagtt gcgtttcgtg acgatccgta tcgacaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa