BrabA.10100.a

Serine protease, V8 family:Serine protease, trypsin family:PDZ/DHR/GLGF domain:HtrA/DegQ protease

CENTER ID:	BrabA.10100.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

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Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.10100.a.A2.PW27663	Mature protein( BrabA.10100.a )		40	513

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126815
PATRIC ID:	fig\|359391.4.peg.664
RefSeq:	YP_414077.1
UniProt:	Q2YMX6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSRARISNYR KGVAAVALSA ALAGAFVVTG PLGALNEARA EAVHVTPPPQ AGFADLVEKV RPAVVSVRVK KDVQETSNRG PQFFGPPGFD QLPDGHPLKR FFRDFGMEPR GDSRSDNRRG KANKPRPGHE RPVAQGSGFV ISEDGYVVTN NHVVSDGDAY TVVLDDGTEL DAKLIGADPR TDLAVLKINA PKRKFVYVAF GDDNKVRVGD WVVAVGNPFG LGGTVTSGIV SARGRDIGAG PYDDFIQIDA AVNKGNSGGP AFDLSGEVIG INTAIFSPSG GSVGIAFAIP SSTAKQVVDQ LIKKGSVERG WIGVQIQPVT KDIAASLGLA EEKGAIVASP QDDGPAAKAG IKAGDVITAV NGETVQDPRD LARKVANIAP GEKAALTVWR KNKAEEINVT IAAMPNDKGK SGSQSNDNDG GQGETLDSYG LTVVPSEDGK GVVVTDVDPD SDAADRGIRS GDVIVSVNNQ TVKTAGDINK AITAAEKSGR KAVLLQLQSN DQSRFVALPI NQE

NT Sequence

ATGTCCAGAG CCAGGATTTC CAACTATCGC AAAGGCGTTG CCGCCGTCGC GCTTTCCGCA GCGCTTGCCG GCGCATTCGT CGTAACCGGC CCGCTTGGTG CGCTGAACGA AGCCCGCGCC GAAGCGGTGC ATGTGACGCC GCCGCCGCAG GCTGGCTTTG CCGATCTGGT GGAAAAGGTT CGCCCTGCGG TCGTCAGCGT CCGCGTCAAG AAGGACGTGC AGGAGACTTC CAACCGTGGT CCGCAGTTCT TCGGCCCTCC CGGCTTCGAC CAGTTGCCGG ACGGTCATCC GCTGAAGCGA TTTTTCCGTG ATTTTGGTAT GGAGCCGCGT GGCGATTCAC GCTCCGATAA CCGTCGCGGC AAGGCCAACA AGCCGCGCCC GGGCCATGAA CGCCCTGTTG CACAAGGATC GGGCTTCGTC ATTTCCGAAG ATGGCTATGT CGTTACCAAC AACCATGTCG TTTCCGATGG CGATGCCTAT ACCGTCGTGC TGGACGACGG CACCGAACTT GATGCCAAGC TGATTGGTGC TGATCCGCGC ACGGACCTGG CCGTGTTGAA AATCAACGCT CCGAAGCGCA AGTTCGTCTA TGTCGCCTTT GGCGACGATA ATAAGGTGCG CGTCGGTGAT TGGGTTGTCG CAGTTGGCAA TCCGTTCGGC CTTGGTGGCA CGGTGACTTC CGGTATCGTT TCGGCCCGTG GCCGAGACAT CGGCGCAGGC CCCTATGACG ATTTCATCCA GATTGATGCC GCCGTGAACA AGGGCAATTC CGGTGGTCCG GCTTTCGACC TTTCCGGCGA AGTCATCGGC ATCAACACCG CCATCTTCTC GCCATCTGGC GGCAGCGTGG GCATTGCTTT TGCAATTCCG TCCTCCACCG CCAAGCAGGT CGTGGACCAG CTCATCAAGA AGGGTTCCGT CGAGCGCGGC TGGATCGGCG TCCAGATACA GCCTGTGACC AAGGATATCG CCGCTTCGCT CGGTCTTGCC GAGGAAAAGG GTGCGATCGT GGCCTCGCCG CAGGATGATG GCCCGGCTGC CAAGGCTGGC ATCAAGGCGG GCGATGTGAT TACGGCTGTC AATGGCGAAA CGGTTCAGGA CCCGCGTGAC CTGGCGCGCA AGGTTGCCAA TATCGCCCCT GGTGAAAAGG CGGCCCTGAC CGTCTGGCGC AAGAACAAGG CCGAGGAAAT CAACGTCACG ATTGCTGCCA TGCCCAATGA CAAGGGGAAG AGCGGCAGCC AGTCCAACGA CAATGACGGC GGACAGGGTG AAACGCTCGA CTCCTACGGC TTGACCGTCG TTCCGTCTGA AGACGGTAAG GGCGTGGTCG TTACCGATGT GGACCCGGAC AGCGATGCGG CTGACCGTGG CATCCGCTCC GGCGATGTGA TCGTGAGCGT CAACAACCAG ACGGTAAAGA CCGCTGGCGA TATCAACAAG GCCATCACGG CAGCCGAAAA GTCAGGCCGT AAGGCGGTGC TGCTGCAATT GCAGAGCAAT GATCAGAGCC GCTTCGTGGC GCTGCCGATC AATCAGGAA

Details for BrabA.10100.a.A2.PW27663

PURIFICATION DATe:	12/28/2009
CONCENTRATION:	28.1mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	85

Protocol Notes

notes unavailable

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAEAVHVTP PPQAGFADLV EKVRPAVVSV RVKKDVQETS NRGPQFFGPP GFDQLPDGHP LKRFFRDFGM EPRGDSRSDN RRGKANKPRP GHERPVAQGS GFVISEDGYV VTNNHVVSDG DAYTVVLDDG TELDAKLIGA DPRTDLAVLK INAPKRKFVY VAFGDDNKVR VGDWVVAVGN PFGLGGTVTS GIVSARGRDI GAGPYDDFIQ IDAAVNKGNS GGPAFDLSGE VIGINTAIFS PSGGSVGIAF AIPSSTAKQV VDQLIKKGSV ERGWIGVQIQ PVTKDIAASL GLAEEKGAIV ASPQDDGPAA KAGIKAGDVI TAVNGETVQD PRDLARKVAN IAPGEKAALT VWRKNKAEEI NVTIAAMPND KGKSGSQSND NDGGQGETLD SYGLTVVPSE DG

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggccg aagcggtgca tgtgacgccg ccgccgcagg ctggctttgc cgatctggtg gaaaaggttc gccctgcggt cgtcagcgtc cgcgtcaaga aggacgtgca ggagacttcc aaccgtggtc cgcagttctt cggccctccc ggcttcgacc agttgccgga cggtcatccg ctgaagcgat ttttccgtga ttttggtatg gagccgcgtg gcgattcacg ctccgataac cgtcgcggca aggccaacaa gccgcgcccg ggccatgaac gccctgttgc acaaggatcg ggcttcgtca tttccgaaga tggctatgtc gttaccaaca accatgtcgt ttccgatggc gatgcctata ccgtcgtgct ggacgacggc accgaacttg atgccaagct gattggtgct gatccgcgca cggacctggc cgtgttgaaa atcaacgctc cgaagcgcaa gttcgtctat gtcgcctttg gcgacgataa taaggtgcgc gtcggtgatt gggttgtcgc agttggcaat ccgttcggcc ttggtggcac ggtgacttcc ggtatcgttt cggcccgtgg ccgagacatc ggcgcaggcc cctatgacga tttcatccag attgatgccg ccgtgaacaa gggcaattcc ggtggtccgg ctttcgacct ttccggcgaa gtcatcggca tcaacaccgc catcttctcg ccatctggcg gcagcgtggg cattgctttt gcaattccgt cctccaccgc caagcaggtc gtggaccagc tcatcaagaa gggttccgtc gagcgcggct ggatcggcgt ccagatacag cctgtgacca aggatatcgc cgcttcgctc ggtcttgccg aggaaaaggg tgcgatcgtg gcctcgccgc aggatgatgg cccggctgcc aaggctggca tcaaggcggg cgatgtgatt acggctgtca atggcgaaac ggttcaggac ccgcgtgacc tggcgcgcaa ggttgccaat atcgcccctg gtgaaaaggc ggccctgacc gtctggcgca agaacaaggc cgaggaaatc aacgtcacga ttgctgccat gcccaatgac aaggggaaga gcggcagcca gtccaacgac aatgacggcg gacagggtga aacgctcgac tcctacggct tgaccgtcgt tccgtctgaa gacggttagg gcgtggtcgt taccgatgtg gacccggaca gcgatgcggc tgaccgtggc atccgctccg gcgatgtgat cgtgagcgtc aacaaccaga cggtaaagac cgctggcgat atcaacaagg ccatcacggc agccgaaaag tcaggccgta aggcggtgct gctgcaattg cagagcaatg atcagagccg cttcgtggcg ctgccgatca at

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGAAGCG GTGCATGTGA CGCCGCCGCC GCAGGCTGGC TTTGCCGATC TGGTGGAAAA GGTTCGCCCT GCGGTCGTCA GCGTCCGCGT CAAGAAGGAC GTGCAGGAGA CTTCCAACCG TGGTCCGCAG TTCTTCGGCC CTCCCGGCTT CGACCAGTTG CCGGACGGTC ATCCGCTGAA GCGATTTTTC CGTGATTTTG GTATGGAGCC GCGTGGCGAT TCACGCTCCG ATAACCGTCG CGGCAAGGCC AACAAGCCGC GCCCGGGCCA TGAACGCCCT GTTGCACAAG GATCGGGCTT CGTCATTTCC GAAGATGGCT ATGTCGTTAC CAACAACCAT GTCGTTTCCG ATGGCGATGC CTATACCGTC GTGCTGGACG ACGGCACCGA ACTTGATGCC AAGCTGATTG GTGCTGATCC GCGCACGGAC CTGGCCGTGT TGAAAATCAA CGCTCCGAAG CGCAAGTTCG TCTATGTCGC CTTTGGCGAC GATAATAAGG TGCGCGTCGG TGATTGGGTT GTCGCAGTTG GCAATCCGTT CGGCCTTGGT GGCACGGTGA CTTCCGGTAT CGTTTCGGCC CGTGGCCGAG ACATCGGCGC AGGCCCCTAT GACGATTTCA TCCAGATTGA TGCCGCCGTG AACAAGGGCA ATTCCGGTGG TCCGGCTTTC GACCTTTCCG GCGAAGTCAT CGGCATCAAC ACCGCCATCT TCTCGCCATC TGGCGGCAGC GTGGGCATTG CTTTTGCAAT TCCGTCCTCC ACCGCCAAGC AGGTCGTGGA CCAGCTCATC AAGAAGGGTT CCGTCGAGCG CGGCTGGATC GGCGTCCAGA TACAGCCTGT GACCAAGGAT ATCGCCGCTT CGCTCGGTCT TGCCGAGGAA AAGGGTGCGA TCGTGGCCTC GCCGCAGGAT GATGGCCCGG CTGCCAAGGC TGGCATCAAG GCGGGCGATG TGATTACGGC TGTCAATGGC GAAACGGTTC AGGACCCGCG TGACCTGGCG CGCAAGGTTG CCAATATCGC CCCTGGTGAA AAGGCGGCCC TGACCGTCTG GCGCAAGAAC AAGGCCGAGG AAATCAACGT CACGATTGCT GCCATGCCCA ATGACAAGGG GAAGAGCGGC AGCCAGTCCA ACGACAATGA CGGCGGACAG GGTGAAACGC TCGACTCCTA CGGCTTGACC GTCGTTCCGT CTGAAGACGG TAAGGGCGTG GTCGTTACCG ATGTGGACCC GGACAGCGAT GCGGCTGACC GTGGCATCCG CTCCGGCGAT GTGATCGTGA GCGTCAACAA CCAGACGGTA AAGACCGCTG GCGATATCAA CAAGGCCATC ACGGCAGCCG AAAAGTCAGG CCGTAAGGCG GTGCTGCTGC AATTGCAGAG CAATGATCAG AGCCGCTTCG TGGCGCTGCC GATCAATCAG GAAAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA