BrabA.10014.p

ATP/GTP-binding site motif A (P-loop):ABC transporter:AAA ATPase

CENTER ID: BrabA.10014.p
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.10014.p.A1.GE27773 Full length( BrabA.10014.p ) 1 316
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.3216
RefSeq: YP_419070.1
UniProt: Q2YJW4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATFGTGSET VVLDKVTKSY GGFNAISDAS FRLMAGENIA LVGHNGAGKT TMIKLMLGLI RPTKGAVRVL GEDPAAGAFA ARLRLGYLPE HVSFNMALTG AETLGFYARL KQVPFGQADA LLERVGLAHA ADRRVGTYSK GMRQRLGLAQ ALLGQPAVLL LDEPTTGLDP ALRQSFYEIL ESLRQEGTTI LMSSHALTEL ETKVGRVIIA NKGKVIADGS IDALRRISRL PMRTRVEVND VSCLPVETGN LLLRWHRLGP DLYETEAPAE GKMELVRRIV AMNDKLNELD IIPPTLDELY AHFLNMGDMA VQEAAE
NT Sequence
ATGGCGACTT TTGGAACAGG CTCCGAGACT GTTGTTCTTG ATAAGGTTAC CAAGTCCTAT GGCGGCTTTA ACGCCATAAG CGATGCAAGT TTCCGGCTTA TGGCGGGGGA AAATATTGCA CTTGTCGGCC ATAATGGCGC GGGCAAGACC ACGATGATCA AGCTCATGCT TGGCCTGATC CGCCCCACCA AAGGCGCGGT GCGTGTGCTG GGTGAAGACC CTGCGGCGGG TGCCTTTGCC GCGCGGTTGC GGCTTGGCTA CCTGCCCGAA CATGTCTCGT TCAACATGGC GCTGACGGGC GCGGAGACAC TTGGCTTCTA TGCCCGGCTG AAACAGGTGC CCTTTGGCCA GGCGGATGCA CTTCTGGAAC GTGTCGGCCT TGCCCATGCT GCCGATCGTC GCGTCGGCAC CTATTCCAAG GGGATGCGCC AACGCCTCGG ACTTGCGCAG GCTTTGCTGG GGCAGCCCGC CGTGCTTTTG CTTGACGAAC CGACCACGGG TCTCGATCCG GCCCTGCGCC AGAGTTTCTA TGAAATTCTG GAAAGCTTGC GGCAGGAGGG CACGACCATT CTCATGTCCT CCCATGCGCT GACCGAGCTT GAAACCAAGG TCGGGCGCGT CATCATCGCC AATAAAGGTA AGGTGATCGC GGATGGTTCC ATCGACGCGC TGCGCCGGAT TTCACGCCTG CCGATGCGTA CCCGTGTGGA GGTGAACGAC GTTTCATGCC TGCCGGTCGA AACCGGCAAC CTGCTGTTGC GCTGGCATCG GCTCGGTCCC GATCTTTACG AAACGGAAGC CCCGGCGGAG GGCAAGATGG AACTTGTACG CCGCATCGTG GCCATGAACG ACAAGCTGAA CGAACTCGAT ATCATCCCGC CCACGCTGGA CGAGCTTTAC GCGCATTTTC TCAATATGGG CGACATGGCG GTTCAGGAGG CGGCAGAA
Details for BrabA.10014.p.A1.GE27773
HARVESTED ON: 11/16/2009
SEQUENCED ON: 11/23/2009
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMATFGTG SETVVLDKVT KSYGGFNAIS DASFRLMAGE NIALVGHNGA GKTTMIKLML GLIRPTKGAV RVLGEDPAAG AFAARLRLGY LPKHVSFNMA LTGAETLGVY ARLKQVPFGQ ADALLERVGL AHAADRRVGT YSKGMRQRLG LAQALLGQPA VLLLDEPTTG LDPALRQSFY EILESLRQEG TTILMSSHAL TELETKVGRV IIANKGKVIA DGSIDALRRI SRLPMRTRVE VNDVSCLPVE TGNMLLRWHR LGPDLYETEA PAEGKMELVR RIVAMNDKLN ELDIIPPTLD ELYAHFLNMG DMAVQEAAE
Validated NT Sequence
ttattattct gccgcctcct gaaccgccat gtcgcccata ttgagaaaat gcgcgtaaag ctcgtccagc gtgggcggga tgatatcgag ttcgttcagc ttgtcgttca tggccacgat gcggcgtaca agttccatct tgccctccgc cggggcttcc gtttcgtaaa gatcgggacc gagccgatgc cagcgcaaca gcatgttgcc ggtttcgacc ggcaggcatg aaacgtcgtt cacctccaca cgggtacgca tcggcaggcg cgaaatccgg cgcagcgcgt cgatggaacc atccgcgatc accttacctt tattggcgat gatgacgcgc ccgaccttgg tttcaagctc ggtcagcgca tgggaggaca tgagaatggt cgtgccctcc tgccgcaagc tttccagaat ttcatagaaa ctctggcgca gggccggatc gagacccgtg gtcggttcgt caagcaaaag cacggcgggc tgccccagca aagcctgcgc aagtccgagg cgttggcgca tccccttgga ataggtgccg acgcgacgat cggcagcatg ggcaaggccg acacgttcca gaagtgcatc cgcctggcca aagggcacct gtttcagccg ggcatagacg ccaagtgtct ccgcgcccgt cagcgccatg ttgaacgaga catgtttggg caggtagcca agccgcaacc gcgcggcaaa ggcacccgcc gcagggtctt cacccagcac acgcaccgcg cctttggtgg ggcggatcag gccaagcatg agcttgatca tcgtggtctt gcccgcgcca ttatggccga caagtgcaat attttccccc gccataagcc ggaaacttgc atcgcttatg gcgttaaagc cgccatagga cttggtaacc ttatcaagaa caacagtctc ggagcctgtt ccaaaagtcg ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMATFGTGSE TVVLDKVTKS YGGFNAISDA SFRLMAGENI ALVGHNGAGK TTMIKLMLGL IRPTKGAVRV LGEDPAAGAF AARLRLGYLP EHVSFNMALT GAETLGFYAR LKQVPFGQAD ALLERVGLAH AADRRVGTYS KGMRQRLGLA QALLGQPAVL LLDEPTTGLD PALRQSFYEI LESLRQEGTT ILMSSHALTE LETKVGRVII ANKGKVIADG SIDALRRISR LPMRTRVEVN DVSCLPVETG NLLLRWHRLG PDLYETEAPA EGKMELVRRI VAMNDKLNEL DIIPPTLDEL YAHFLNMGDM AVQEAAE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgactttt ggaacaggct ccgagactgt tgttcttgat aaggttacca agtcctatgg cggctttaac gccataagcg atgcaagttt ccggcttatg gcgggggaaa atattgcact tgtcggccat aatggcgcgg gcaagaccac gatgatcaag ctcatgcttg gcctgatccg ccccaccaaa ggcgcggtgc gtgtgctggg tgaagaccct gcggcgggtg cctttgccgc gcggttgcgg cttggctacc tgcccgaaca tgtctcgttc aacatggcgc tgacgggcgc ggagacactt ggcttctatg cccggctgaa acaggtgccc tttggccagg cggatgcact tctggaacgt gtcggccttg cccatgctgc cgatcgtcgc gtcggcacct attccaaggg gatgcgccaa cgcctcggac ttgcgcaggc tttgctgggg cagcccgccg tgcttttgct tgacgaaccg accacgggtc tcgatccggc cctgcgccag agtttctatg aaattctgga aagcttgcgg caggagggca cgaccattct catgtcctcc catgcgctga ccgagcttga aaccaaggtc gggcgcgtca tcatcgccaa taaaggtaag gtgatcgcgg atggttccat cgacgcgctg cgccggattt cacgcctgcc gatgcgtacc cgtgtggagg tgaacgacgt ttcatgcctg ccggtcgaaa ccggcaacct gctgttgcgc tggcatcggc tcggtcccga tctttacgaa acggaagccc cggcggaggg caagatggaa cttgtacgcc gcatcgtggc catgaacgac aagctgaacg aactcgatat catcccgccc acgctggacg agctttacgc gcattttctc aatatgggcg acatggcggt tcaggaggcg gcagaaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa