SSGCID: Target Page

BrabA.10014.o

ATP/GTP-binding site motif A (P-loop):ABC transporter:AAA ATPase

CENTER ID:	BrabA.10014.o
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.10014.o.A1.GE27772	Full length( BrabA.10014.o )		1	234

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.3121
RefSeq:	YP_418981.1
UniProt:	Q2YK53

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLTLEHVDAG YGRTTILQDV SLEVKEGEIV TIVGANGAGK TTTLRTIVGQ IQPRAGKITF LGEDITHLPA HEVVDRGIIL IPEGRQLFPD MTVRENLQMG TYRRAARAAQ ESRMEEVLEL FPRVRERLDQ TASSLSGGEQ QMVAIARGMM ANPRILMFDE PSLGLAPIVV SQVFDVVRKI AATGTTVLIV EQNVFTTLKV ANRGYVLENG RVVLADSSEA LLNNDHVRRA YLGI

NT Sequence

ATGCTCACGC TTGAACATGT CGATGCGGGT TATGGTCGGA CCACAATCCT TCAGGATGTC AGCCTTGAAG TAAAGGAAGG CGAGATCGTC ACCATTGTTG GCGCGAACGG CGCCGGCAAG ACCACGACGC TGCGCACTAT TGTTGGCCAG ATACAGCCAC GCGCTGGCAA GATAACTTTT CTCGGCGAGG ATATTACCCA TCTTCCAGCC CATGAGGTCG TAGACAGGGG CATCATCCTT ATTCCGGAAG GGCGCCAGCT CTTTCCCGAC ATGACGGTGC GGGAAAATCT CCAGATGGGA ACCTATCGCC GCGCAGCCCG CGCCGCGCAG GAAAGCCGGA TGGAGGAGGT GCTGGAGCTG TTTCCCCGCG TGCGTGAGCG TCTGGACCAG ACGGCGTCGT CGCTTTCCGG CGGCGAACAG CAGATGGTGG CGATCGCGCG CGGCATGATG GCCAATCCAC GCATATTGAT GTTTGACGAA CCATCGCTGG GCCTTGCTCC CATTGTGGTT TCACAAGTCT TCGACGTCGT GCGGAAGATA GCAGCCACCG GAACGACAGT TCTTATCGTC GAGCAGAATG TGTTTACGAC GCTGAAAGTC GCGAACCGTG GCTATGTACT TGAAAATGGG CGGGTTGTTC TGGCCGACAG TTCGGAAGCA CTTTTGAACA ACGATCATGT GCGCCGTGCT TATCTGGGAA TT

Details for BrabA.10014.o.A1.GE27772

HARVESTED ON:	11/16/2009
SEQUENCED ON:	11/23/2009
EXPECTED MW:	27kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLTLEHVDA GYGRTTILQD VSLEVKEGEI VTIVGANGAG KTTTLRTIVG QIQPRAGKIT FLGEDITHLP AHEVVDRGII LIPEGRQLFP DMTVRENLQM GTYRRAARAA QESRMEEVLE LFPRVRERLD QTASSLSGGE QQMVAIARGM MANPRILMFD EPSLGLAPIV VSQVFDVVRK IAATGTTVLI VEQNVFTTLK VANRGYVLEN GRVVLADSSE ALLNNDHVRR AYLGI

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctca cgcttgaaca tgtcgatgcg ggttatggtc ggaccacaat ccttcaggat gtcagccttg aagtaaagga aggcgagatc gtcaccattg ttggcgcgaa cggcgccggc aagaccacga cgctgcgcac tattgttggc cagatacagc cacgcgctgg caagataact tttctcggcg aggatattac ccatcttcca gcccatgagg tcgtagacag gggcatcatc cttattccgg aagggcgcca gctctttccc gacatgacgg tgcgggaaaa tctccagatg ggaacctatc gccgcgcagc ccgcgccgcg caggaaagcc ggatggagga ggtgctggag ctgtttcccc gcgtgcgtga gcgtctggac cagacggcgt cgtcgctttc cggcggcgaa cagcagatgg tggcgatcgc gcgcggcatg atggccaatc cacgcatatt gatgtttgac gaaccatcgc tgggccttgc tcccattgtg gtttcacaag tcttcgacgt cgtgcggaag atagcagcca ccggaacgac agttcttatc gtcgagcaga atgtgtttac gacgctgaaa gtcgcgaacc gtggctatgt acttgaaaat gggcgggttg ttctggccga cagttcggaa gcacttttga acaacgatca tgtgcgccgt gcttatctgg gaatttaata a

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctcacgctt gaacatgtcg atgcgggtta tggtcggacc acaatccttc aggatgtcag ccttgaagta aaggaaggcg agatcgtcac cattgttggc gcgaacggcg ccggcaagac cacgacgctg cgcactattg ttggccagat acagccacgc gctggcaaga taacttttct cggcgaggat attacccatc ttccagccca tgaggtcgta gacaggggca tcatccttat tccggaaggg cgccagctct ttcccgacat gacggtgcgg gaaaatctcc agatgggaac ctatcgccgc gcagcccgcg ccgcgcagga aagccggatg gaggaggtgc tggagctgtt tccccgcgtg cgtgagcgtc tggaccagac ggcgtcgtcg ctttccggcg gcgaacagca gatggtggcg atcgcgcgcg gcatgatggc caatccacgc atattgatgt ttgacgaacc atcgctgggc cttgctccca ttgtggtttc acaagtcttc gacgtcgtgc ggaagatagc agccaccgga acgacagttc ttatcgtcga gcagaatgtg tttacgacgc tgaaagtcgc gaaccgtggc tatgtacttg aaaatgggcg ggttgttctg gccgacagtt cggaagcact tttgaacaac gatcatgtgc gccgtgctta tctgggaatt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa