SSGCID: Target Page

BrabA.10014.n

ATP/GTP-binding site motif A (P-loop):ABC transporter:AAA ATPase

CENTER ID:	BrabA.10014.n
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.10014.n.A1.GE27771	Full length( BrabA.10014.n )		1	333

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.10014.n.A1.PS00464	Full length( BrabA.10014.n )		1	333
BrabA.10014.n.A1.PW27659	Full length( BrabA.10014.n )		1	333

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.3013
RefSeq:	YP_418891.1
UniProt:	Q2YKE3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGSLQLKSVH KRYGAQEVLK DINLEVNDGE FIIFVGPSGC GKSTLLRSIA GLEDVSAGQV LINGEDVTVT PPSRRGIAMV FQSYALYPHL TVKANMGLGL KQAGAPKDEI EGRVAKASAM LALEPYLARR PAELSGGQRQ RVAIGRALVR NPELFLFDEP LSNLDAALRV QTRLEIAKLH RELKATMIYV THDQVEAMTL ADRIVVLNAG RIEQIGSPME LYNRPDNLFV AGFIGSPQMN FIEAARIGAT GARTIGIRPE HLSVSRESGT WKGKVIHAEH LGADTILYVE TETAGLVTAR LFGEQHYNED DVIFLTPEEG KTHRFDEAGK AIR

NT Sequence

GTGGGATCGC TGCAACTCAA ATCGGTACAT AAGCGCTACG GTGCACAGGA AGTTCTCAAG GACATCAATC TGGAAGTGAA TGACGGGGAG TTCATCATCT TCGTCGGTCC TTCCGGTTGC GGTAAATCAA CCCTGCTGCG CTCCATCGCG GGTCTCGAAG ACGTGTCCGC CGGGCAGGTG CTCATCAATG GTGAGGACGT GACCGTCACG CCGCCTTCCA GACGTGGCAT TGCCATGGTG TTCCAGTCTT ATGCGCTTTA TCCGCATCTG ACCGTTAAGG CCAATATGGG GCTTGGTCTC AAACAGGCCG GTGCGCCGAA AGATGAGATT GAAGGCCGTG TGGCCAAGGC TTCCGCCATG CTGGCGCTGG AGCCTTACCT TGCGCGTCGC CCGGCGGAAC TTTCAGGCGG CCAGCGCCAG CGCGTCGCCA TTGGGCGCGC ATTGGTGCGC AACCCGGAAT TGTTCCTTTT CGACGAACCG CTGTCGAACC TTGATGCCGC GCTTCGTGTG CAGACCCGGC TTGAAATTGC CAAATTGCAT CGTGAGTTGA AAGCAACGAT GATCTATGTG ACCCACGATC AGGTCGAGGC AATGACGCTC GCCGACCGTA TCGTGGTGCT GAATGCCGGC CGCATAGAGC AGATCGGCTC CCCGATGGAG CTTTATAACC GCCCGGATAA TCTGTTCGTT GCCGGTTTTA TCGGCTCGCC GCAGATGAAC TTCATTGAGG CCGCGCGCAT CGGCGCGACG GGCGCCAGGA CGATTGGCAT TCGCCCCGAA CATCTTTCTG TCAGCCGCGA GAGCGGAACG TGGAAAGGCA AGGTCATCCA TGCCGAGCAT CTGGGTGCCG ATACGATCCT CTATGTCGAG ACAGAAACGG CAGGTCTTGT CACTGCCCGC CTGTTCGGTG AGCAGCACTA TAATGAAGAT GATGTGATCT TCCTCACCCC GGAAGAAGGC AAGACCCATC GTTTCGATGA GGCAGGCAAA GCGATCCGC

Details for BrabA.10014.n.A1.GE27771

HARVESTED ON:	11/16/2009
SEQUENCED ON:	11/23/2009
EXPECTED MW:	38kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMVGSLQL KSVHKRYGAQ EVLKDINLEV NDGEFIIFVG PSGCGKSTLL RSIAGLEDVS AGQVLINGED VTVTPPSRRG IAMVFQSYAL YPHLTVKANM GLGLKQAGAP KDEIEGRVAK ASAMLALEPY LARRPAELSG GQRQRVAIGR ALVRNPELFL FDEPLSNLDA ALRVQTRLEI AKLHRELKAT MIYVTHDQVE AMTLADRIVV LNAGRIEQIG SPMELYNRPD NLFVAGFIGS PQMNFIEAAR IGATGARTIG IRPEHLSVSR ESGTWKGKVI HAEHLGADTI LYVETETAGL VTARLFGEQH YNEDDVIFLT PEEGKTHRFD EAGKAIR

Validated NT Sequence

caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgggatcg ctgcaactca aatcggtaca taagcgctac ggtgcacagg aagttctcaa ggacatcaat ctggaagtga atgacgggga gttcatcatc ttcgtcggtc cttccggttg cggtaaatca accctgctgc gctccatcgc gggtctcgaa gacgtgtccg ccgggcaggt gctcatcaat ggtgaggacg tgaccgtcac gccgccttcc agacgtggca ttgccatggt gttccagtct tatgcgcttt atccgcatct gaccgttaag gccaatatgg ggcttggtct caaacaggcc ggtgcgccga aagatgagat tgaaggccgt gtggccaagg cttccgccat gctggcgctg gagccttacc ttgcgcgtcg cccggcggaa ctttcaggcg gccagcgcca gcgcgtcgcc attgggcgcg cattggtgcg caacccggaa ttgttccttt tcgacgaacc gctgtcgaac cttgatgccg cgcttcgtgt gcagacccgg cttgaaattg ccaaattgca tcgtgagttg aaagcaacga tgatctatgt gacccacgat caggtcgagg caatgacgct cgccgaccgt atcgtggtgc tgaatgccgg ccgcatagag cagatcggct ccccgatgga gctttataac cgcccggata atctgttcgt tgccggtttt atcggctcgc cgcagatgaa cttcattgag gccgcgcgca tcggcgcgac gggcgccagg acgattggca ttcgccccga acatctttct gtcagccgcg agagcggaac gtggaaaggc aaggtcatcc atgccgagca tctgggtgcc gatacgatcc tctatgtcga gacagaaacg gcaggtcttg tcactgcccg cctgttcggt gagcagcact ataatgaaga tgatgtgatc ttcctcaccc cggaagaagg caagacccat cgtttcgatg aggcaggcaa agcgatccgc taataa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVGSLQLKS VHKRYGAQEV LKDINLEVND GEFIIFVGPS GCGKSTLLRS IAGLEDVSAG QVLINGEDVT VTPPSRRGIA MVFQSYALYP HLTVKANMGL GLKQAGAPKD EIEGRVAKAS AMLALEPYLA RRPAELSGGQ RQRVAIGRAL VRNPELFLFD EPLSNLDAAL RVQTRLEIAK LHRELKATMI YVTHDQVEAM TLADRIVVLN AGRIEQIGSP MELYNRPDNL FVAGFIGSPQ MNFIEAARIG ATGARTIGIR PEHLSVSRES GTWKGKVIHA EHLGADTILY VETETAGLVT ARLFGEQHYN EDDVIFLTPE EGKTHRFDEA GKAIR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgggatcg ctgcaactca aatcggtaca taagcgctac ggtgcacagg aagttctcaa ggacatcaat ctggaagtga atgacgggga gttcatcatc ttcgtcggtc cttccggttg cggtaaatca accctgctgc gctccatcgc gggtctcgaa gacgtgtccg ccgggcaggt gctcatcaat ggtgaggacg tgaccgtcac gccgccttcc agacgtggca ttgccatggt gttccagtct tatgcgcttt atccgcatct gaccgttaag gccaatatgg ggcttggtct caaacaggcc ggtgcgccga aagatgagat tgaaggccgt gtggccaagg cttccgccat gctggcgctg gagccttacc ttgcgcgtcg cccggcggaa ctttcaggcg gccagcgcca gcgcgtcgcc attgggcgcg cattggtgcg caacccggaa ttgttccttt tcgacgaacc gctgtcgaac cttgatgccg cgcttcgtgt gcagacccgg cttgaaattg ccaaattgca tcgtgagttg aaagcaacga tgatctatgt gacccacgat caggtcgagg caatgacgct cgccgaccgt atcgtggtgc tgaatgccgg ccgcatagag cagatcggct ccccgatgga gctttataac cgcccggata atctgttcgt tgccggtttt atcggctcgc cgcagatgaa cttcattgag gccgcgcgca tcggcgcgac gggcgccagg acgattggca ttcgccccga acatctttct gtcagccgcg agagcggaac gtggaaaggc aaggtcatcc atgccgagca tctgggtgcc gatacgatcc tctatgtcga gacagaaacg gcaggtcttg tcactgcccg cctgttcggt gagcagcact ataatgaaga tgatgtgatc ttcctcaccc cggaagaagg caagacccat cgtttcgatg aggcaggcaa agcgatccgc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for BrabA.10014.n.A1.PS00464

PURIFICATION DATe:	1/26/2010
CONCENTRATION:	18.4mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGGATCG CTGCAACTCA AATCGGTACA TAAGCGCTAC GGTGCACAGG AAGTTCTCAA GGACATCAAT CTGGAAGTGA ATGACGGGGA GTTCATCATC TTCGTCGGTC CTTCCGGTTG CGGTAAATCA ACCCTGCTGC GCTCCATCGC GGGTCTCGAA GACGTGTCCG CCGGGCAGGT GCTCATCAAT GGTGAGGACG TGACCGTCAC GCCGCCTTCC AGACGTGGCA TTGCCATGGT GTTCCAGTCT TATGCGCTTT ATCCGCATCT GACCGTTAAG GCCAATATGG GGCTTGGTCT CAAACAGGCC GGTGCGCCGA AAGATGAGAT TGAAGGCCGT GTGGCCAAGG CTTCCGCCAT GCTGGCGCTG GAGCCTTACC TTGCGCGTCG CCCGGCGGAA CTTTCAGGCG GCCAGCGCCA GCGCGTCGCC ATTGGGCGCG CATTGGTGCG CAACCCGGAA TTGTTCCTTT TCGACGAACC GCTGTCGAAC CTTGATGCCG CGCTTCGTGT GCAGACCCGG CTTGAAATTG CCAAATTGCA TCGTGAGTTG AAAGCAACGA TGATCTATGT GACCCACGAT CAGGTCGAGG CAATGACGCT CGCCGACCGT ATCGTGGTGC TGAATGCCGG CCGCATAGAG CAGATCGGCT CCCCGATGGA GCTTTATAAC CGCCCGGATA ATCTGTTCGT TGCCGGTTTT ATCGGCTCGC CGCAGATGAA CTTCATTGAG GCCGCGCGCA TCGGCGCGAC GGGCGCCAGG ACGATTGGCA TTCGCCCCGA ACATCTTTCT GTCAGCCGCG AGAGCGGAAC GTGGAAAGGC AAGGTCATCC ATGCCGAGCA TCTGGGTGCC GATACGATCC TCTATGTCGA GACAGAAACG GCAGGTCTTG TCACTGCCCG CCTGTTCGGT GAGCAGCACT ATAATGAAGA TGATGTGATC TTCCTCACCC CGGAAGAAGG CAAGACCCAT CGTTTCGATG AGGCAGGCAA AGCGATCCGC AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA

Details for BrabA.10014.n.A1.PW27659

PURIFICATION DATe:	6/28/2010
CONCENTRATION:	3.41mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)