BrabA.10014.m

Disease resistance protein:ATP/GTP-binding site motif A (P-loop):ABC transporter:AAA ATPase

CENTER ID: BrabA.10014.m
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.10014.m.A1.GE27770 Full length( BrabA.10014.m ) 1 351
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_128894
PATRIC ID: fig|359391.4.peg.2876
RefSeq: YP_418766.1
UniProt: Q2YKR8
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKIVLDNVR KSYGGNIEVI KGVSLEIADG EFVVLVGPSG CGKSTLLRMI AGLESITSGT ISIGERVVNN VEPAERDIAM VFQNYALYPH MTVRENLAYG LKNRKTPKEE IERRIAKAAK ALEIEQFLER KPRQLSGGQR QRVAMGRAIV REPAAFLFDE PLSNLDAKLR VQMRVEIKRL QRSLGTTSVY VTHDQMEAMT MADRLVVLNA GHIEQVGTPI ELYEKPASTF VATFIGSPSM NLLQSPESAP WQPGRAITLP SGGYTFGVRP EDIRILEEGD QDADGFNAQV RIEAVELVGA ESYIHAALSD GKPLIFRVAG RSTHNIDEMV RVGASATDVH IFGADGRRVS D
NT Sequence
ATGAGCAAAA TCGTTCTTGA TAACGTCCGC AAGAGCTATG GCGGCAATAT CGAAGTCATC AAAGGCGTTT CGCTGGAGAT CGCGGATGGT GAATTCGTGG TGCTGGTTGG CCCGTCGGGC TGTGGCAAGT CCACGCTGCT GCGCATGATC GCGGGCCTCG AAAGCATCAC TTCCGGCACC ATTTCCATCG GCGAGCGCGT GGTCAATAAT GTGGAACCCG CCGAACGCGA TATCGCCATG GTGTTCCAGA ATTATGCGCT TTATCCGCAT ATGACGGTGC GCGAAAACCT CGCCTATGGC CTGAAAAACC GCAAAACACC GAAAGAAGAG ATCGAACGCC GCATCGCCAA GGCCGCAAAG GCGCTGGAGA TCGAGCAGTT TCTGGAGCGC AAGCCCCGCC AGCTCTCCGG TGGCCAGCGC CAGCGCGTCG CCATGGGCCG CGCCATCGTG CGCGAACCGG CCGCCTTCCT CTTCGATGAG CCGCTTTCCA ACCTCGACGC AAAGCTGCGC GTGCAGATGC GCGTAGAGAT CAAGCGCCTG CAACGCTCGC TCGGCACCAC CAGCGTCTAT GTCACCCACG ACCAGATGGA AGCCATGACC ATGGCCGACC GTCTCGTGGT GCTCAATGCC GGTCATATCG AGCAGGTCGG CACGCCGATC GAGCTTTATG AAAAGCCCGC CTCGACATTC GTGGCGACCT TCATCGGCTC ACCCTCGATG AACCTGCTTC AAAGCCCAGA ATCTGCCCCC TGGCAACCGG GCCGCGCAAT CACGCTTCCC TCCGGCGGGT ACACTTTCGG TGTGCGCCCG GAAGATATCC GCATTCTCGA AGAGGGTGAT CAGGATGCCG ATGGCTTCAA CGCGCAAGTG CGCATTGAAG CAGTCGAACT GGTTGGCGCG GAAAGCTATA TTCATGCCGC GCTCAGCGAC GGCAAGCCGC TGATCTTCCG CGTTGCGGGC CGCTCCACGC ATAATATTGA TGAAATGGTC CGCGTTGGTG CATCCGCCAC GGATGTACAT ATTTTCGGCG CGGACGGACG CCGCGTGAGC GAC
Details for BrabA.10014.m.A1.GE27770
HARVESTED ON: 11/16/2009
SEQUENCED ON: 11/23/2009
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSKIVLDNV RKSYGGNIEV IKGVSLEIAD GEFVVLVGPS GCGKSTLLRM IAGLESITSG TISIGERVVN NVEPAERDIA MVFQNYALYP HMTVRENLAY GLKNRKTPKE EIERRIAKAA KALEIEQFLE RKPRQLSGGQ RQRVAMGRAI VREPAAFLFD EPLSNLDAKL RVQMRVEIKR LQRSLGTTSV YVTHDQMEAM TMADRLVVLN AGHIEQVGTP IELYEKPAST FVATFIGSPS MNLLQSPESA PWQPGRAITL PSGGYTFGVR PEDIRILEEG DQDADGFNAQ VRIEAVELVG AESYIHAALS DGKPLIFRVA GRSTHNIDEM VRVGASATDV HIFGADGRRV SD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagca aaatcgttct tgataacgtc cgcaagagct atggcggcaa tatcgaagtc atcaaaggcg tttcgctgga gatcgcggat ggtgaattcg tggtgctggt tggcccgtcg ggctgtggca agtccacgct gctgcgcatg atcgcgggcc tcgaaagcat cacttccggc accatttcca tcggcgagcg cgtggtcaat aatgtggaac ccgccgaacg cgatatcgcc atggtgttcc agaattatgc gctttatccg catatgacgg tgcgcgaaaa cctcgcctat ggcctgaaaa accgcaaaac accgaaagaa gagatcgaac gccgcatcgc caaggccgca aaggcgctgg agatcgagca gtttctggag cgcaagcccc gccagctctc cggtggccag cgccagcgcg tcgccatggg ccgcgccatc gtgcgcgaac cggccgcctt cctcttcgat gagccgcttt ccaacctcga cgcaaagctg cgcgtgcaga tgcgcgtaga gatcaagcgc ctgcaacgct cgctcggcac caccagcgtc tatgtcaccc acgaccagat ggaagccatg accatggccg accgtctcgt ggtgctcaat gccggtcata tcgagcaggt cggcacgccg atcgagcttt atgaaaagcc cgcctcgaca ttcgtggcga ccttcatcgg ctcaccctcg atgaacctgc ttcaaagccc agaatctgcc ccctggcaac cgggccgcgc aatcacgctt ccctccggcg ggtacacttt cggtgtgcgc ccggaagata tccgcattct cgaagagggt gatcaggatg ccgatggctt caacgcgcaa gtgcgcattg aagcagtcga actggttggc gcggaaagct atattcatgc cgcgctcagc gacggcaagc cgctgatctt ccgcgttgcg ggccgctcca cgcataatat tgatgaaatg gtccgcgttg gtgcatccgc cacggatgta catattttcg gcgcggacgg acgccgcgtg agcgactaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSKIVLDNV RKSYGGNIEV IKGVSLEIAD GEFVVLVGPS GCGKSTLLRM IAGLESITSG TISIGERVVN NVEPAERDIA MVFQNYALYP HMTVRENLAY GLKNRKTPKE EIERRIAKAA KALEIEQFLE RKPRQLSGGQ RQRVAMGRAI VREPAAFLFD EPLSNLDAKL RVQMRVEIKR LQRSLGTTSV YVTHDQMEAM TMADRLVVLN AGHIEQVGTP IELYEKPAST FVATFIGSPS MNLLQSPESA PWQPGRAITL PSGGYTFGVR PEDIRILEEG DQDADGFNAQ VRIEAVELVG AESYIHAALS DGKPLIFRVA GRSTHNIDEM VRVGASATDV HIFGADGRRV SD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcaaaatc gttcttgata acgtccgcaa gagctatggc ggcaatatcg aagtcatcaa aggcgtttcg ctggagatcg cggatggtga attcgtggtg ctggttggcc cgtcgggctg tggcaagtcc acgctgctgc gcatgatcgc gggcctcgaa agcatcactt ccggcaccat ttccatcggc gagcgcgtgg tcaataatgt ggaacccgcc gaacgcgata tcgccatggt gttccagaat tatgcgcttt atccgcatat gacggtgcgc gaaaacctcg cctatggcct gaaaaaccgc aaaacaccga aagaagagat cgaacgccgc atcgccaagg ccgcaaaggc gctggagatc gagcagtttc tggagcgcaa gccccgccag ctctccggtg gccagcgcca gcgcgtcgcc atgggccgcg ccatcgtgcg cgaaccggcc gccttcctct tcgatgagcc gctttccaac ctcgacgcaa agctgcgcgt gcagatgcgc gtagagatca agcgcctgca acgctcgctc ggcaccacca gcgtctatgt cacccacgac cagatggaag ccatgaccat ggccgaccgt ctcgtggtgc tcaatgccgg tcatatcgag caggtcggca cgccgatcga gctttatgaa aagcccgcct cgacattcgt ggcgaccttc atcggctcac cctcgatgaa cctgcttcaa agcccagaat ctgccccctg gcaaccgggc cgcgcaatca cgcttccctc cggcgggtac actttcggtg tgcgcccgga agatatccgc attctcgaag agggtgatca ggatgccgat ggcttcaacg cgcaagtgcg cattgaagca gtcgaactgg ttggcgcgga aagctatatt catgccgcgc tcagcgacgg caagccgctg atcttccgcg ttgcgggccg ctccacgcat aatattgatg aaatggtccg cgttggtgca tccgccacgg atgtacatat tttcggcgcg gacggacgcc gcgtgagcga caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa