SSGCID: Target Page

BrabA.10014.l

ATP/GTP-binding site motif A (P-loop):ABC transporter:AAA ATPase

CENTER ID:	BrabA.10014.l
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.10014.l.A1.GE27769	Full length( BrabA.10014.l )		1	371

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.2848
RefSeq:	YP_418737.1
UniProt:	Q2YKU7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSFLKITDLY KSYGSVSVLK DINIEIDEGG FLVLVGPSGC GKSTLLNTIA GLEPITSGDI AINGKSVSNL HPSQRDIAMV FQSYALYPNM TVAGNIAFGM EIRKVPKPER DKAIQQVADM LQIGHLLDRK PSQLSGGQRR RVAMGRALVR NPQVFLFDEP LSNLDAKLRV DMRTEIKRLH SRMKTTIVYV THDQIEAMTL ATKIAVLKDG VLQQFGTPAE IYNNPANMFV ADFMGSPAMN LLPVKVEKSG SDYAITLDQG EGEVLKLPLK AVPQGLAQYV GKEVVFGIRP EALTDPDGAD RNAASIVEGD CLIDVVEPAG SDTFAVTRLG GKHVVARLRA DARIRAGQSA RLAFNLDKSV FFDSASQTRI L

NT Sequence

ATGTCATTTT TGAAAATTAC CGACCTCTAC AAATCCTATG GCAGCGTATC GGTCCTGAAG GACATCAATA TCGAGATTGA TGAAGGCGGG TTTCTGGTGC TCGTCGGCCC ATCCGGCTGC GGCAAGTCCA CATTGTTGAA TACGATTGCC GGGCTGGAGC CGATCACCTC CGGTGATATT GCGATCAATG GCAAATCGGT TTCCAACCTG CACCCCTCGC AGCGCGATAT TGCCATGGTC TTCCAGTCCT ATGCGCTTTA TCCCAACATG ACAGTGGCGG GAAACATTGC CTTTGGCATG GAAATTCGCA AAGTGCCAAA GCCGGAGCGC GACAAGGCCA TTCAGCAAGT GGCGGATATG TTGCAGATCG GTCATCTGCT CGACCGCAAG CCAAGCCAGC TTTCGGGCGG CCAGCGCCGG CGCGTTGCCA TGGGGCGCGC GCTTGTCCGC AACCCGCAGG TCTTCCTGTT CGATGAACCC TTATCTAATC TCGATGCCAA ACTGCGTGTC GACATGCGCA CCGAGATCAA GCGCCTTCAC AGCCGCATGA AGACGACCAT CGTTTATGTG ACTCATGACC AGATCGAAGC CATGACGCTC GCGACCAAAA TCGCGGTTCT GAAAGATGGC GTTCTTCAAC AGTTCGGTAC ACCGGCGGAA ATTTATAACA ATCCGGCCAA TATGTTCGTA GCGGACTTCA TGGGATCGCC TGCCATGAAC CTTCTGCCCG TCAAGGTGGA GAAGAGCGGT TCGGATTATG CCATTACGCT CGATCAGGGC GAGGGCGAGG TTTTGAAGCT GCCGCTGAAG GCCGTACCGC AGGGGCTTGC GCAATATGTC GGCAAGGAAG TGGTTTTCGG CATTCGCCCG GAAGCCCTGA CGGACCCGGA CGGGGCCGAC CGCAACGCGG CCTCGATTGT CGAGGGCGAC TGCCTGATTG ACGTGGTGGA GCCTGCCGGT TCGGATACGT TTGCGGTGAC GCGCCTCGGC GGCAAGCATG TGGTTGCGCG TCTGCGCGCA GATGCGCGCA TCCGCGCCGG CCAGTCGGCC CGGCTTGCCT TCAATCTGGA CAAGTCGGTG TTCTTCGATT CCGCGAGCCA GACGCGCATC CTG

Details for BrabA.10014.l.A1.GE27769

HARVESTED ON:	11/16/2009
SEQUENCED ON:	11/23/2009
EXPECTED MW:	42kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

cctggaagct cagacccagg gtcctggttc gatgtcattt ttgaaaatta ccgacctcta caaatcctat ggcagcgtat cggtcctgaa ggacatcaat atcgagattg atgaaggcgg gtttctggtg ctcgtcggcc catccggctg cggcaagtcc acattgttga atacgattgc cgggctggag ccgatcacct ccggtgatat tgcgatcaat ggcaaatcgg tttccaacct gcacccctcg cagcgcgata ttgccatggt cttccagtcc tatgcgcttt atcccaacat gacagtggcg ggaaacattg cctttggcat ggaaattcgc aaagtgccaa agccggagcg cgacaaggcc attcagcaag tggcggatat gttgcagatc ggtcatctgc tcgaccgcaa gccaagccag ctttcgggcg gccagcgccg gcgcgttgcc atggggcgcg cgcttgtccg caacccgcag gtcttcctgt tcgatgaacc cttatctaat ctcgatgcca aactgcgtgt cgacatgcgc accgagatca agcgccttca cagccgcatg aagacgacca tcgtttatgt gactcatgac cagatcgaag ccatgacgct cgcgaccaaa atcgcggttc tgaaagatgg cgttcttcaa cagttcggta caccggcgga aatttataac aatccggcca atatgttcgt agcggacttc atgggatcgc ctgccatgaa ccttctgccc gtcaaggtgg agaagagcgg ttcggattat gccattacgc tcgatcaggg cgagggcgag gttttgaagc tgccgctgaa ggccgtaccg caggggcttg cgcaatatgt cggcaaggaa gtggttttcg gcattcgccc ggaagccctg acggacccgg acggggccga ccgcaacgcg gcctcgattg tcgagggcga ctgcctgatt gacgtggtgg agcctgccgg ttcggatacg tttgcggtga cgcgcctcgg cggcaagcat gtggttgcgc gtctgcgcgc agatgcgcgc atccgcgccg gccagtcggc ccggcttgcc ttcaatctgg acaagtcggt gttcttcgat tccgcgagcc agacgcgcat cctgt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSFLKITDL YKSYGSVSVL KDINIEIDEG GFLVLVGPSG CGKSTLLNTI AGLEPITSGD IAINGKSVSN LHPSQRDIAM VFQSYALYPN MTVAGNIAFG MEIRKVPKPE RDKAIQQVAD MLQIGHLLDR KPSQLSGGQR RRVAMGRALV RNPQVFLFDE PLSNLDAKLR VDMRTEIKRL HSRMKTTIVY VTHDQIEAMT LATKIAVLKD GVLQQFGTPA EIYNNPANMF VADFMGSPAM NLLPVKVEKS GSDYAITLDQ GEGEVLKLPL KAVPQGLAQY VGKEVVFGIR PEALTDPDGA DRNAASIVEG DCLIDVVEPA GSDTFAVTRL GGKHVVARLR ADARIRAGQS ARLAFNLDKS VFFDSASQTR IL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcatttttg aaaattaccg acctctacaa atcctatggc agcgtatcgg tcctgaagga catcaatatc gagattgatg aaggcgggtt tctggtgctc gtcggcccat ccggctgcgg caagtccaca ttgttgaata cgattgccgg gctggagccg atcacctccg gtgatattgc gatcaatggc aaatcggttt ccaacctgca cccctcgcag cgcgatattg ccatggtctt ccagtcctat gcgctttatc ccaacatgac agtggcggga aacattgcct ttggcatgga aattcgcaaa gtgccaaagc cggagcgcga caaggccatt cagcaagtgg cggatatgtt gcagatcggt catctgctcg accgcaagcc aagccagctt tcgggcggcc agcgccggcg cgttgccatg gggcgcgcgc ttgtccgcaa cccgcaggtc ttcctgttcg atgaaccctt atctaatctc gatgccaaac tgcgtgtcga catgcgcacc gagatcaagc gccttcacag ccgcatgaag acgaccatcg tttatgtgac tcatgaccag atcgaagcca tgacgctcgc gaccaaaatc gcggttctga aagatggcgt tcttcaacag ttcggtacac cggcggaaat ttataacaat ccggccaata tgttcgtagc ggacttcatg ggatcgcctg ccatgaacct tctgcccgtc aaggtggaga agagcggttc ggattatgcc attacgctcg atcagggcga gggcgaggtt ttgaagctgc cgctgaaggc cgtaccgcag gggcttgcgc aatatgtcgg caaggaagtg gttttcggca ttcgcccgga agccctgacg gacccggacg gggccgaccg caacgcggcc tcgattgtcg agggcgactg cctgattgac gtggtggagc ctgccggttc ggatacgttt gcggtgacgc gcctcggcgg caagcatgtg gttgcgcgtc tgcgcgcaga tgcgcgcatc cgcgccggcc agtcggcccg gcttgccttc aatctggaca agtcggtgtt cttcgattcc gcgagccaga cgcgcatcct gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa