SSGCID: Target Page

BrabA.10014.j

ATP/GTP-binding site motif A (P-loop):ABC transporter:AAA ATPase:Glycine betaine/L-proline transport ATP-binding subunit

CENTER ID:	BrabA.10014.j
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.10014.j.A1.GE27767	Full length( BrabA.10014.j )		1	377

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.2796
RefSeq:	YP_418690.1
UniProt:	Q2YKZ4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MACFKVFGDN PMRAMRELGA GKSKAQIHSD LGATIGVDNA TFDIREGEVF VIMGLSGSGK STLLRLLNRL IEPTAGSIEV EGRDIVKMSK RELIDLRRRD MSMVFQSFAL LPNRSVLNNA AFGLEVAGMG EVQRHQKALK ALAAVGLEPY AHSMPDQLSG GMKQRVGLAR ALASEPTVLL MDEAFSALDP LIRTEMQDEL KRLQAQHSRT IVFVSHDLDE AMRIGDRICI MQHGKVVQVG TPNEIISAPA NDYVRSFFRN VDVSRVFKAA DVARDDELIV FEAEQLATAL ERFDASGKAY GVLVDADRTY RGLVSRDALA AGQRLNDFAA IEADAPLSGL LVHVAKSPWP VPVTDRQNRY VGAISRSVLL ETLGRAG

NT Sequence

ATGGCGTGTT TTAAAGTCTT CGGCGACAAC CCGATGCGTG CAATGCGTGA ATTGGGTGCG GGCAAATCAA AAGCGCAGAT TCACAGCGAT CTCGGGGCCA CGATTGGCGT TGACAATGCC ACCTTCGATA TTCGGGAAGG TGAAGTATTC GTGATTATGG GCCTTTCCGG CTCGGGCAAA TCCACACTTC TGCGTCTGCT CAATCGGCTT ATCGAACCAA CGGCCGGTTC CATTGAAGTG GAAGGCCGCG ATATCGTCAA AATGTCGAAG CGTGAGCTGA TCGATCTTCG CCGCCGCGAT ATGAGCATGG TGTTCCAGTC ATTCGCGCTG CTTCCCAACC GTTCGGTGCT GAACAATGCC GCTTTCGGTC TTGAAGTGGC AGGCATGGGC GAGGTTCAGC GGCACCAGAA AGCGTTGAAG GCGCTGGCCG CTGTGGGGCT GGAGCCCTAT GCGCACAGCA TGCCGGACCA GCTTTCCGGC GGCATGAAAC AGCGTGTCGG CCTGGCGCGG GCGCTTGCCA GTGAGCCGAC AGTTCTTCTG ATGGACGAAG CCTTTTCCGC GCTCGATCCA CTGATCCGCA CTGAAATGCA GGACGAGCTG AAGCGGCTTC AGGCGCAACA CAGCCGCACT ATTGTTTTCG TGAGTCACGA TCTGGACGAG GCCATGCGCA TCGGTGACCG TATCTGCATC ATGCAGCACG GCAAGGTGGT GCAGGTGGGA ACGCCAAACG AGATCATATC GGCCCCGGCC AATGATTATG TCCGTTCTTT CTTCCGTAAT GTCGATGTTT CGCGGGTTTT CAAGGCCGCA GACGTGGCGC GGGATGACGA ACTCATCGTC TTTGAAGCCG AACAGCTTGC CACGGCGCTG GAGCGTTTCG ACGCAAGCGG CAAGGCCTAT GGCGTACTGG TCGATGCCGA CCGCACCTAT CGTGGGCTTG TGAGCCGCGA TGCGCTGGCC GCGGGCCAAA GGCTCAACGA TTTTGCGGCC ATCGAAGCCG ATGCGCCGCT TTCCGGCTTG CTTGTGCATG TGGCGAAAAG CCCCTGGCCG GTGCCTGTTA CCGACCGGCA GAACCGCTAT GTCGGCGCGA TCAGCAGGTC GGTCTTGCTT GAAACGCTCG GGCGCGCGGG C

Details for BrabA.10014.j.A1.GE27767

HARVESTED ON:	11/16/2009
SEQUENCED ON:	11/23/2009
EXPECTED MW:	43kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

tattagcccg cgcgcccgag cgtttcaagc aagaccgacc tgctgatcgc gccgacatag cggttctgcc ggtcggtaac aggcaccggc caggggcttt tcgccacatg cacaagcaag ccggaaagcg gcgcatcggc ttcgatggcc gcaaaatcgt tgagcctttg gcccgcggcc agcgcatcgc ggctcacaag cccacgatag gtgcggtcgg catcgaccag tacgccatag gccttgccgc ttgcgtcgaa acgctccagc gccgtggcaa gctgttcggc ttcaaagacg atgagttcgt catcccgcgc cacgtctgcg gccttgaaaa cccgcgaaac atcgacatta cggaagaaag aacggacata atcattggcc ggggccgata tgatctcgtt tggcgttccc acctgcacca ccttgccgtg ctgcatgatg cagatacggt caccgatgcg catggcctcg tccagatcgt gactcacgaa aacaatggtg cggctgtgtt gcgcctgaag ccgcttcagc tcgtcctgca tttcagtgcg gatcagtgga tcgagcgcgg aaaaggcttc gtccatcaga agaactgtcg gctcactggc aagcgcccgc gccaggccga cacgctgttt catgccgccg gaaagctggt ccggcatgct gtgcgcatag ggctccagcc ccacagcggc cagcgccttc aacgctttct ggtgccgctg aacctcgccc atgcctgcca cttcaagacc gaaagcggca ttgttcagca ccgaacggtt gggaagcagc gcgaatgact ggaacaccat gctcatatcg cggcggcgaa gatcgatcag ctcacgcttc gacattttga cgatatcgcg gccttccact tcaatggaac cggccgttgg ttcgataagc cgattgagca gacgcagaag tgtggatttg cccgagccgg aaaggcccat aatcacgaat acttcacctt cccgaatatc gaaggtggca ttgtcaacgc caatcgtggc cccgagatcg ctgtgaatct gcgcttttga tttgcccgca cccaattcac gcattgcacg catcgggttg tcgccgaaga ctttaaaaca cgccatcgaa ccaggaccct gggtctgagc ttccaggg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMACFKVFGD NPMRAMRELG AGKSKAQIHS DLGATIGVDN ATFDIREGEV FVIMGLSGSG KSTLLRLLNR LIEPTAGSIE VEGRDIVKMS KRELIDLRRR DMSMVFQSFA LLPNRSVLNN AAFGLEVAGM GEVQRHQKAL KALAAVGLEP YAHSMPDQLS GGMKQRVGLA RALASEPTVL LMDEAFSALD PLIRTEMQDE LKRLQAQHSR TIVFVSHDLD EAMRIGDRIC IMQHGKVVQV GTPNEIISAP ANDYVRSFFR NVDVSRVFKA ADVARDDELI VFEAEQLATA LERFDASGKA YGVLVDADRT YRGLVSRDAL AAGQRLNDFA AIEADAPLSG LLVHVAKSPW PVPVTDRQNR YVGAISRSVL LETLGRAG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgtgtttt aaagtcttcg gcgacaaccc gatgcgtgca atgcgtgaat tgggtgcggg caaatcaaaa gcgcagattc acagcgatct cggggccacg attggcgttg acaatgccac cttcgatatt cgggaaggtg aagtattcgt gattatgggc ctttccggct cgggcaaatc cacacttctg cgtctgctca atcggcttat cgaaccaacg gccggttcca ttgaagtgga aggccgcgat atcgtcaaaa tgtcgaagcg tgagctgatc gatcttcgcc gccgcgatat gagcatggtg ttccagtcat tcgcgctgct tcccaaccgt tcggtgctga acaatgccgc tttcggtctt gaagtggcag gcatgggcga ggttcagcgg caccagaaag cgttgaaggc gctggccgct gtggggctgg agccctatgc gcacagcatg ccggaccagc tttccggcgg catgaaacag cgtgtcggcc tggcgcgggc gcttgccagt gagccgacag ttcttctgat ggacgaagcc ttttccgcgc tcgatccact gatccgcact gaaatgcagg acgagctgaa gcggcttcag gcgcaacaca gccgcactat tgttttcgtg agtcacgatc tggacgaggc catgcgcatc ggtgaccgta tctgcatcat gcagcacggc aaggtggtgc aggtgggaac gccaaacgag atcatatcgg ccccggccaa tgattatgtc cgttctttct tccgtaatgt cgatgtttcg cgggttttca aggccgcaga cgtggcgcgg gatgacgaac tcatcgtctt tgaagccgaa cagcttgcca cggcgctgga gcgtttcgac gcaagcggca aggcctatgg cgtactggtc gatgccgacc gcacctatcg tgggcttgtg agccgcgatg cgctggccgc gggccaaagg ctcaacgatt ttgcggccat cgaagccgat gcgccgcttt ccggcttgct tgtgcatgtg gcgaaaagcc cctggccggt gcctgttacc gaccggcaga accgctatgt cggcgcgatc agcaggtcgg tcttgcttga aacgctcggg cgcgcgggca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a