SSGCID: Target Page

BrabA.10014.h

ATP/GTP-binding site motif A (P-loop):ABC transporter:AAA ATPase

CENTER ID:	BrabA.10014.h
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.10014.h.A1.GE27765	Full length( BrabA.10014.h )		1	251

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.2040
RefSeq:	YP_415294.1
UniProt:	Q2YLW0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHLSAKKTSS AVAMRAVSKW YGQFQVLKDI DFDVARGERV VICGPSGSGK STLIRCINRL EPYQDGQIIV DGIALTDEAK SVDEVRREVG MVFQHFNLFP HLTILENCTL APMSVRGMSR KAATEIAMNY LSRVMIADHS HKYPSQISGG QQQRAAIARA LCMNPKIMLF DEPTSALDPE MVSEVLDTMV SLAEEGMTMI CVTHEMGFAR RVADRVIFMD QGNIIESAEP SIFFDAPKHE RTRLFLSQIL H

NT Sequence

ATGCATCTGT CGGCTAAGAA GACCTCCAGT GCCGTCGCCA TGCGCGCGGT CAGCAAATGG TACGGGCAAT TTCAGGTTCT CAAGGACATT GATTTCGATG TCGCCAGAGG CGAACGGGTG GTGATTTGCG GTCCATCGGG ATCGGGAAAA TCCACCCTGA TCCGCTGCAT CAACCGGCTG GAGCCTTATC AGGACGGCCA GATTATCGTC GATGGCATCG CACTCACAGA TGAGGCGAAA TCGGTGGACG AGGTGCGGCG CGAAGTGGGC ATGGTGTTCC AGCATTTCAA TCTCTTTCCC CATCTGACCA TTCTGGAAAA CTGTACGCTC GCCCCCATGT CGGTGCGCGG CATGAGCCGC AAGGCCGCGA CCGAGATCGC CATGAATTAT CTGTCCCGCG TCATGATCGC GGATCACAGT CACAAATATC CGTCGCAGAT CTCCGGCGGG CAGCAGCAGC GCGCAGCCAT TGCCCGCGCG CTCTGCATGA ACCCGAAAAT CATGCTTTTC GACGAGCCAA CCTCGGCCCT CGACCCGGAA ATGGTATCGG AGGTGCTGGA CACCATGGTC AGCCTTGCTG AAGAGGGGAT GACCATGATC TGCGTGACCC ATGAAATGGG CTTTGCGCGC CGGGTCGCCG ACCGCGTCAT CTTCATGGAT CAGGGCAACA TCATTGAAAG CGCGGAACCA TCCATATTCT TCGACGCGCC GAAACATGAA CGGACCCGGC TCTTTCTCAG CCAGATATTG CAT

Details for BrabA.10014.h.A1.GE27765

HARVESTED ON:	11/16/2009
SEQUENCED ON:	11/23/2009
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMHLSAKKTS SAVAMRAVSK WYGQFQVLKD IDFDVARGER VVICGPSGSG KSTLIRCINR LEPYQDGQII VDGIALTDEA KSVDEVRREV GMVFQHFNLF PHLTILENCT LAPMSVRGMS RKAATEIAMN YLSRVMIADH SHKYPSQISG GQQQRAAIAR ALCMNPKIML FDEPTSALDP EMVSEVLDTM VSLAEEGMTM ICVTHEMGFA RRVADRVIFM DQGNIIESAE PSIFFDAPKH ERTRLFLSQI LH

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcatc tgtcggctaa gaagacctcc agtgccgtcg ccatgcgcgc ggtcagcaaa tggtacgggc aatttcaggt tctcaaggac attgatttcg atgtcgccag aggcgaacgg gtggtgattt gcggtccatc gggatcggga aaatccaccc tgatccgctg catcaaccgg ctggagcctt atcaggacgg ccagattatc gtcgatggca tcgcactcac agatgaggcg aaatcggtgg acgaggtgcg gcgcgaagtg ggcatggtgt tccagcattt caatctcttt ccccatctga ccattctgga aaactgtacg ctcgccccca tgtcggtgcg cggcatgagc cgcaaggccg cgaccgagat cgccatgaat tatctgtccc gcgtcatgat cgcggatcac agtcacaaat atccgtcgca gatctccggc gggcagcagc agcgcgcagc cattgcccgc gcgctctgca tgaacccgaa aatcatgctt ttcgacgagc caacctcggc cctcgacccg gaaatggtat cggaggtgct ggacaccatg gtcagccttg ctgaagaggg gatgaccatg atctgcgtga cccatgaaat gggctttgcg cgccgggtcg ccgaccgcgt catcttcatg gatcagggca acatcattga aagcgcggaa ccatccatat tcttcgacgc gccgaaacat gaacggaccc ggctctttct cagccagata ttgcattaat aa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcatctgtcg gctaagaaga cctccagtgc cgtcgccatg cgcgcggtca gcaaatggta cgggcaattt caggttctca aggacattga tttcgatgtc gccagaggcg aacgggtggt gatttgcggt ccatcgggat cgggaaaatc caccctgatc cgctgcatca accggctgga gccttatcag gacggccaga ttatcgtcga tggcatcgca ctcacagatg aggcgaaatc ggtggacgag gtgcggcgcg aagtgggcat ggtgttccag catttcaatc tctttcccca tctgaccatt ctggaaaact gtacgctcgc ccccatgtcg gtgcgcggca tgagccgcaa ggccgcgacc gagatcgcca tgaattatct gtcccgcgtc atgatcgcgg atcacagtca caaatatccg tcgcagatct ccggcgggca gcagcagcgc gcagccattg cccgcgcgct ctgcatgaac ccgaaaatca tgcttttcga cgagccaacc tcggccctcg acccggaaat ggtatcggag gtgctggaca ccatggtcag ccttgctgaa gaggggatga ccatgatctg cgtgacccat gaaatgggct ttgcgcgccg ggtcgccgac cgcgtcatct tcatggatca gggcaacatc attgaaagcg cggaaccatc catattcttc gacgcgccga aacatgaacg gacccggctc tttctcagcc agatattgca taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa