SSGCID: Target Page

BrabA.10014.g

ATP/GTP-binding site motif A (P-loop):ABC transporter:AAA ATPase

CENTER ID:	BrabA.10014.g
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.10014.g.A1.GE27764	Full length( BrabA.10014.g )		1	271

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.2037
RefSeq:	YP_415291.1
UniProt:	Q2YLW3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTSPLLRISN LAKSFGNVTA LRDISFDVSA GEVVCIIGPS GCGKSTLLRC INHLTAPDGG FVEVGGEYIG RERKPDGTVR MQSHHEIDLM RPKMGFVFQQ FNLWPHLSVL ENIVKGPIKV QKRPRAEAEA QALALLQRFG LRDKAHMFPA GLSGGQKQRV AIARALAMGP ELMLFDEPTS ALDPEIVKEV LLFLRELADS GVTMVIVTHE IGFARHVADR IVFLDKGVVA ETGPSEDVLS NPANPRLREF LSQISSNHFH AGGAFSPAAT H

NT Sequence

ATGACCAGCC CCCTGCTCAG GATTTCCAAT CTGGCCAAAA GCTTTGGCAA TGTGACTGCC CTTCGCGATA TCAGCTTCGA TGTATCAGCG GGTGAAGTGG TCTGCATCAT CGGCCCCAGC GGCTGTGGCA AGAGCACGCT ATTGCGCTGC ATCAATCATC TCACCGCACC TGACGGGGGC TTCGTCGAAG TTGGCGGCGA ATATATCGGC CGCGAACGCA AGCCTGACGG AACGGTGCGC ATGCAGTCGC ATCATGAAAT CGACCTCATG CGCCCGAAAA TGGGTTTCGT GTTCCAGCAG TTTAATCTGT GGCCCCATTT GAGCGTTCTG GAAAACATCG TGAAAGGCCC GATCAAGGTG CAGAAACGCC CCCGGGCGGA AGCGGAAGCA CAGGCGCTCG CCCTGTTGCA GCGTTTCGGC CTGCGCGACA AGGCCCATAT GTTTCCCGCC GGGCTTTCGG GCGGGCAGAA GCAGCGCGTC GCCATTGCAC GCGCGCTTGC CATGGGGCCG GAGCTGATGC TTTTCGATGA GCCGACATCC GCGCTCGATC CCGAGATCGT CAAGGAAGTG CTGCTTTTCC TGCGTGAGCT TGCCGATTCC GGCGTCACGA TGGTGATCGT CACGCATGAA ATCGGCTTTG CGCGCCACGT CGCAGACCGG ATCGTCTTTC TGGACAAGGG CGTCGTCGCC GAAACCGGCC CTTCCGAGGA CGTGCTTTCC AACCCCGCCA ATCCGCGCCT GCGGGAGTTT CTCTCGCAGA TTTCATCCAA TCATTTCCAT GCAGGCGGCG CGTTTTCGCC CGCTGCAACG CAT

Details for BrabA.10014.g.A1.GE27764

HARVESTED ON:	11/16/2009
SEQUENCED ON:	11/23/2009
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMTSPLLRIS NLAKSFGNVT ALRDISFDVS AGEVVCIIGP SGCGKSTLLR CINHLTAPDG GFVEVGGEYI GRERKPDGTV RMQSHHEIDL MRPKMGFVFQ QFNLWPHLSV LENIVKGPIK VQKRPRAEAE AQALALLQRF GLRDKAHMFP AGLSGGQKQR VAIARALAMG PELMLFDEPT SALDPEIVKE VLLFLRELAD SGVTMVIVTH EIGFARHVAD RIVFLDKGVV AETGPSEDVL SNPANPRLRE FLSQISSNHF HAGGAFSPAA TH

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacca gccccctgct caggatttcc aatctggcca aaagctttgg caatgtgact gcccttcgcg atatcagctt cgatgtatca gcgggtgaag tggtctgcat catcggcccc agcggctgtg gcaagagcac gctattgcgc tgcatcaatc atctcaccgc acctgacggg ggcttcgtcg aagttggcgg cgaatatatc ggccgcgaac gcaagcctga cggaacggtg cgcatgcagt cgcatcatga aatcgacctc atgcgcccga aaatgggttt cgtgttccag cagtttaatc tgtggcccca tttgagcgtt ctggaaaaca tcgtgaaagg cccgatcaag gtgcagaaac gcccccgggc ggaagcggaa gcacaggcgc tcgccctgtt gcagcgtttc ggcctgcgcg acaaggccca tatgtttccc gccgggcttt cgggcgggca gaagcagcgc gtcgccattg cacgcgcgct tgccatgggg ccggagctga tgcttttcga tgagccgaca tccgcgctcg atcccgagat cgtcaaggaa gtgctgcttt tcctgcgtga gcttgccgat tccggcgtca cgatggtgat cgtcacgcat gaaatcggct ttgcgcgcca cgtcgcagac cggatcgtct ttctggacaa gggcgtcgtc gccgaaaccg gcccttccga ggacgtgctt tccaaccccg ccaatccgcg cctgcgggag tttctctcgc agatttcatc caatcatttc catgcaggcg gcgcgttttc gcccgctgca acgcattaat aa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccagcccc ctgctcagga tttccaatct ggccaaaagc tttggcaatg tgactgccct tcgcgatatc agcttcgatg tatcagcggg tgaagtggtc tgcatcatcg gccccagcgg ctgtggcaag agcacgctat tgcgctgcat caatcatctc accgcacctg acgggggctt cgtcgaagtt ggcggcgaat atatcggccg cgaacgcaag cctgacggaa cggtgcgcat gcagtcgcat catgaaatcg acctcatgcg cccgaaaatg ggtttcgtgt tccagcagtt taatctgtgg ccccatttga gcgttctgga aaacatcgtg aaaggcccga tcaaggtgca gaaacgcccc cgggcggaag cggaagcaca ggcgctcgcc ctgttgcagc gtttcggcct gcgcgacaag gcccatatgt ttcccgccgg gctttcgggc gggcagaagc agcgcgtcgc cattgcacgc gcgcttgcca tggggccgga gctgatgctt ttcgatgagc cgacatccgc gctcgatccc gagatcgtca aggaagtgct gcttttcctg cgtgagcttg ccgattccgg cgtcacgatg gtgatcgtca cgcatgaaat cggctttgcg cgccacgtcg cagaccggat cgtctttctg gacaagggcg tcgtcgccga aaccggccct tccgaggacg tgctttccaa ccccgccaat ccgcgcctgc gggagtttct ctcgcagatt tcatccaatc atttccatgc aggcggcgcg ttttcgcccg ctgcaacgca taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa