BrabA.10014.f

ATP/GTP-binding site motif A (P-loop):ABC transporter:AAA ATPase:Spermidine/putrescine ABC transporter ATP-binding subunit

CENTER ID: BrabA.10014.f
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.10014.f.A1.GE27763 Full length( BrabA.10014.f ) 1 381
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.10014.f.A1.PS00469 Full length( BrabA.10014.f ) 1 381
BrabA.10014.f.A1.PW27651 Full length( BrabA.10014.f ) 1 381

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_127485
PATRIC ID: fig|359391.4.peg.1685
RefSeq: YP_414987.1
UniProt: Q2YQA2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MESFGSFRRK FAPWNDPAAK PFISFENVTK IFGDFTAVDN LSLNVFNREF FALLGASGCG KTTLMRMLAG FEEPTSGRIT LDGQDMRGIP PYKRPVNMMF QSYALFPHMT VEKNIAFGLK QDGMAKSEID ARVAEMLKLV KLEQYGKRKP HQLSGGQRQR VALARSVAKR PKVLLLDEPL GALDKKLREE TQFELMDLQV KLGMTFMVVT HDQEEAMTMA DRIAVMDKGR IMQVATPAEV YEAPRSKFVA DFIGNVNIIE GEVVKAANGE AEIATEKFGF HIHTETREQL NPGQKVWYAV RPEKTRISRE KPDEAAVNAV EGELWDIAYF GDMTVFHVRL DNGRTVKAAS LNAVRKTENP LTYDERVWVS FDIDAGLVLT A
NT Sequence
ATGGAATCTT TTGGCAGCTT TCGCCGCAAA TTCGCGCCGT GGAACGACCC GGCGGCCAAG CCTTTTATTT CTTTCGAGAA TGTGACGAAA ATTTTCGGTG ATTTCACTGC CGTCGATAAT CTGTCGCTCA ATGTTTTCAA TCGGGAATTC TTTGCGCTGC TTGGTGCGTC GGGTTGCGGC AAGACCACGC TGATGCGTAT GCTGGCGGGC TTCGAGGAGC CGACATCGGG CCGCATTACG CTCGATGGTC AGGATATGCG CGGCATCCCG CCCTATAAGC GCCCCGTCAA CATGATGTTC CAGTCCTATG CACTGTTCCC GCATATGACG GTGGAGAAGA ATATCGCCTT CGGTCTCAAA CAGGATGGCA TGGCGAAATC GGAGATCGAT GCCCGTGTCG CTGAAATGCT CAAGCTCGTG AAGCTGGAGC AATATGGCAA GCGCAAGCCA CATCAGTTAT CCGGCGGCCA GCGCCAGCGC GTGGCGCTGG CCCGTTCCGT CGCCAAGCGC CCGAAGGTGT TGCTGCTCGA CGAGCCGCTT GGTGCGCTCG ACAAAAAGCT GCGCGAGGAA ACCCAGTTTG AATTGATGGA TTTGCAGGTC AAGCTTGGCA TGACCTTCAT GGTTGTCACC CACGATCAGG AAGAAGCCAT GACCATGGCC GACCGTATCG CGGTCATGGA CAAGGGTCGC ATCATGCAGG TGGCAACGCC TGCCGAAGTC TATGAAGCGC CGCGCTCCAA ATTCGTGGCC GATTTCATCG GCAATGTGAA TATCATCGAA GGCGAGGTGG TGAAGGCGGC AAACGGCGAG GCGGAAATCG CCACCGAGAA ATTCGGCTTC CACATTCACA CCGAAACGCG CGAACAGCTC AATCCCGGCC AGAAGGTCTG GTATGCGGTG CGCCCGGAAA AGACCCGCAT CTCGCGCGAG AAACCCGACG AGGCCGCCGT CAATGCGGTG GAGGGCGAGC TTTGGGATAT TGCCTATTTC GGCGACATGA CGGTCTTTCA CGTGCGTCTC GACAATGGCC GCACTGTCAA GGCAGCGAGC CTCAATGCCG TGCGCAAGAC TGAAAACCCG CTCACCTATG ACGAGCGCGT CTGGGTTTCA TTCGATATCG ATGCCGGTCT GGTGCTGACG GCC
Details for BrabA.10014.f.A1.GE27763
HARVESTED ON: 11/16/2009
SEQUENCED ON: 11/23/2009
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMESFGSFRR KFAPWNDPAA KPFISFENVT KIFGDFTAVD NLSLNVFNRE FFALLGASGC GKTTLMRMLA GFEEPTSGRI TLDGQDMRGI PPYKRPVNMM FQSYALFPHM TVEKNIAFGL KQDGMAKSEI DARVAEMLKL VKLEQYGKRK PHQLSGGQRQ RVALARSVAK RPKVLLLDEP LGALDKKLRE ETQFELMDLQ VKLGMTFMVV THDQEEAMTM ADRIAVMDKG RIMQVATPAE VYEAPRSKFV ADFIGNVNII EGEVVKAANG EAEIATEKFG FHIHTETREQ LNPGQKVWYA VRPEKTRISR EKPDEAAVNA VEGELWDIAY FGDMTVFHVR LDNGRTVKAA SLNAVRKTEN PLTYDERVWV SFDIDAGLVL TA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaat cttttggcag ctttcgccgc aaattcgcgc cgtggaacga cccggcggcc aagcctttta tttctttcga gaatgtgacg aaaattttcg gtgatttcac tgccgtcgat aatctgtcgc tcaatgtttt caatcgggaa ttctttgcgc tgcttggtgc gtcgggttgc ggcaagacca cgctgatgcg tatgctggcg ggcttcgagg agccgacatc gggccgcatt acgctcgatg gtcaggatat gcgcggcatc ccgccctata agcgccccgt caacatgatg ttccagtcct atgcactgtt cccgcatatg acggtggaga agaatatcgc cttcggtctc aaacaggatg gcatggcgaa atcggagatc gatgcccgtg tcgctgaaat gctcaagctc gtgaagctgg agcaatatgg caagcgcaag ccacatcagt tatccggcgg ccagcgccag cgcgtggcgc tggcccgttc cgtcgccaag cgcccgaagg tgttgctgct cgacgagccg cttggtgcgc tcgacaaaaa gctgcgcgag gaaacccagt ttgaattgat ggatttgcag gtcaagcttg gcatgacctt catggttgtc acccacgatc aggaagaagc catgaccatg gccgaccgta tcgcggtcat ggacaagggt cgcatcatgc aggtggcaac gcctgccgaa gtctatgaag cgccgcgctc caaattcgtg gccgatttca tcggcaatgt gaatatcatc gaaggcgagg tggtgaaggc ggcaaacggc gaggcggaaa tcgccaccga gaaattcggc ttccacattc acaccgaaac gcgcgaacag ctcaatcccg gccagaaggt ctggtatgcg gtgcgcccgg aaaagacccg catctcgcgc gagaaacccg acgaggccgc cgtcaatgcg gtggagggcg agctttggga tattgcctat ttcggcgaca tgacggtctt tcacgtgcgt ctcgacaatg gccgcactgt caaggcagcg agcctcaatg ccgtgcgcaa gactgaaaac ccgctcacct atgacgagcg cgtctgggtt tcattcgata tcgatgccgg tctggtgctg acggcctaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMESFGSFRR KFAPWNDPAA KPFISFENVT KIFGDFTAVD NLSLNVFNRE FFALLGASGC GKTTLMRMLA GFEEPTSGRI TLDGQDMRGI PPYKRPVNMM FQSYALFPHM TVEKNIAFGL KQDGMAKSEI DARVAEMLKL VKLEQYGKRK PHQLSGGQRQ RVALARSVAK RPKVLLLDEP LGALDKKLRE ETQFELMDLQ VKLGMTFMVV THDQEEAMTM ADRIAVMDKG RIMQVATPAE VYEAPRSKFV ADFIGNVNII EGEVVKAANG EAEIATEKFG FHIHTETREQ LNPGQKVWYA VRPEKTRISR EKPDEAAVNA VEGELWDIAY FGDMTVFHVR LDNGRTVKAA SLNAVRKTEN PLTYDERVWV SFDIDAGLVL TA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaatctttt ggcagctttc gccgcaaatt cgcgccgtgg aacgacccgg cggccaagcc ttttatttct ttcgagaatg tgacgaaaat tttcggtgat ttcactgccg tcgataatct gtcgctcaat gttttcaatc gggaattctt tgcgctgctt ggtgcgtcgg gttgcggcaa gaccacgctg atgcgtatgc tggcgggctt cgaggagccg acatcgggcc gcattacgct cgatggtcag gatatgcgcg gcatcccgcc ctataagcgc cccgtcaaca tgatgttcca gtcctatgca ctgttcccgc atatgacggt ggagaagaat atcgccttcg gtctcaaaca ggatggcatg gcgaaatcgg agatcgatgc ccgtgtcgct gaaatgctca agctcgtgaa gctggagcaa tatggcaagc gcaagccaca tcagttatcc ggcggccagc gccagcgcgt ggcgctggcc cgttccgtcg ccaagcgccc gaaggtgttg ctgctcgacg agccgcttgg tgcgctcgac aaaaagctgc gcgaggaaac ccagtttgaa ttgatggatt tgcaggtcaa gcttggcatg accttcatgg ttgtcaccca cgatcaggaa gaagccatga ccatggccga ccgtatcgcg gtcatggaca agggtcgcat catgcaggtg gcaacgcctg ccgaagtcta tgaagcgccg cgctccaaat tcgtggccga tttcatcggc aatgtgaata tcatcgaagg cgaggtggtg aaggcggcaa acggcgaggc ggaaatcgcc accgagaaat tcggcttcca cattcacacc gaaacgcgcg aacagctcaa tcccggccag aaggtctggt atgcggtgcg cccggaaaag acccgcatct cgcgcgagaa acccgacgag gccgccgtca atgcggtgga gggcgagctt tgggatattg cctatttcgg cgacatgacg gtctttcacg tgcgtctcga caatggccgc actgtcaagg cagcgagcct caatgccgtg cgcaagactg aaaacccgct cacctatgac gagcgcgtct gggtttcatt cgatatcgat gccggtctgg tgctgacggc caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BrabA.10014.f.A1.PS00469
PURIFICATION DATe: 1/29/2010
CONCENTRATION: 12.2mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMESFGSFRR KFAPWNDPAA KPFISFENVT KIFGDFTAVD NLSLNVFNRE FFALLGASGC GKTTLMRMLA GFEEPTSGRI TLDGQDMRGI PPYKRPVNMM FQSYALFPHM TVEKNIAFGL KQDGMAKSEI DARVAEMLKL VKLEQYGKRK PHQLSGGQRQ RVALARSVAK RPKVLLLDEP LGALDKKLRE ETQFELMDLQ VKLGMTFMVV THDQEEAMTM ADRIAVMDKG RIMQVATPAE VYEAPRSKFV ADFIGNVNII EGEVVKAANG EAEIATEKFG FHIHTETREQ LNPGQKVWYA VRPEKTRISR EKPDEAAVNA VEGELWDIAY FGDMTVFHVR LDNGRTVKAA SLNAVRKTEN PLTYDERVWV SFDIDAGLVL TA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaat cttttggcag ctttcgccgc aaattcgcgc cgtggaacga cccggcggcc aagcctttta tttctttcga gaatgtgacg aaaattttcg gtgatttcac tgccgtcgat aatctgtcgc tcaatgtttt caatcgggaa ttctttgcgc tgcttggtgc gtcgggttgc ggcaagacca cgctgatgcg tatgctggcg ggcttcgagg agccgacatc gggccgcatt acgctcgatg gtcaggatat gcgcggcatc ccgccctata agcgccccgt caacatgatg ttccagtcct atgcactgtt cccgcatatg acggtggaga agaatatcgc cttcggtctc aaacaggatg gcatggcgaa atcggagatc gatgcccgtg tcgctgaaat gctcaagctc gtgaagctgg agcaatatgg caagcgcaag ccacatcagt tatccggcgg ccagcgccag cgcgtggcgc tggcccgttc cgtcgccaag cgcccgaagg tgttgctgct cgacgagccg cttggtgcgc tcgacaaaaa gctgcgcgag gaaacccagt ttgaattgat ggatttgcag gtcaagcttg gcatgacctt catggttgtc acccacgatc aggaagaagc catgaccatg gccgaccgta tcgcggtcat ggacaagggt cgcatcatgc aggtggcaac gcctgccgaa gtctatgaag cgccgcgctc caaattcgtg gccgatttca tcggcaatgt gaatatcatc gaaggcgagg tggtgaaggc ggcaaacggc gaggcggaaa tcgccaccga gaaattcggc ttccacattc acaccgaaac gcgcgaacag ctcaatcccg gccagaaggt ctggtatgcg gtgcgcccgg aaaagacccg catctcgcgc gagaaacccg acgaggccgc cgtcaatgcg gtggagggcg agctttggga tattgcctat ttcggcgaca tgacggtctt tcacgtgcgt ctcgacaatg gccgcactgt caaggcagcg agcctcaatg ccgtgcgcaa gactgaaaac ccgctcacct atgacgagcg cgtctgggtt tcattcgata tcgatgccgg tctggtgctg acggcctaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMESFGSFRR KFAPWNDPAA KPFISFENVT KIFGDFTAVD NLSLNVFNRE FFALLGASGC GKTTLMRMLA GFEEPTSGRI TLDGQDMRGI PPYKRPVNMM FQSYALFPHM TVEKNIAFGL KQDGMAKSEI DARVAEMLKL VKLEQYGKRK PHQLSGGQRQ RVALARSVAK RPKVLLLDEP LGALDKKLRE ETQFELMDLQ VKLGMTFMVV THDQEEAMTM ADRIAVMDKG RIMQVATPAE VYEAPRSKFV ADFIGNVNII EGEVVKAANG EAEIATEKFG FHIHTETREQ LNPGQKVWYA VRPEKTRISR EKPDEAAVNA VEGELWDIAY FGDMTVFHVR LDNGRTVKAA SLNAVRKTEN PLTYDERVWV SFDIDAGLVL TA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAATCTTTT GGCAGCTTTC GCCGCAAATT CGCGCCGTGG AACGACCCGG CGGCCAAGCC TTTTATTTCT TTCGAGAATG TGACGAAAAT TTTCGGTGAT TTCACTGCCG TCGATAATCT GTCGCTCAAT GTTTTCAATC GGGAATTCTT TGCGCTGCTT GGTGCGTCGG GTTGCGGCAA GACCACGCTG ATGCGTATGC TGGCGGGCTT CGAGGAGCCG ACATCGGGCC GCATTACGCT CGATGGTCAG GATATGCGCG GCATCCCGCC CTATAAGCGC CCCGTCAACA TGATGTTCCA GTCCTATGCA CTGTTCCCGC ATATGACGGT GGAGAAGAAT ATCGCCTTCG GTCTCAAACA GGATGGCATG GCGAAATCGG AGATCGATGC CCGTGTCGCT GAAATGCTCA AGCTCGTGAA GCTGGAGCAA TATGGCAAGC GCAAGCCACA TCAGTTATCC GGCGGCCAGC GCCAGCGCGT GGCGCTGGCC CGTTCCGTCG CCAAGCGCCC GAAGGTGTTG CTGCTCGACG AGCCGCTTGG TGCGCTCGAC AAAAAGCTGC GCGAGGAAAC CCAGTTTGAA TTGATGGATT TGCAGGTCAA GCTTGGCATG ACCTTCATGG TTGTCACCCA CGATCAGGAA GAAGCCATGA CCATGGCCGA CCGTATCGCG GTCATGGACA AGGGTCGCAT CATGCAGGTG GCAACGCCTG CCGAAGTCTA TGAAGCGCCG CGCTCCAAAT TCGTGGCCGA TTTCATCGGC AATGTGAATA TCATCGAAGG CGAGGTGGTG AAGGCGGCAA ACGGCGAGGC GGAAATCGCC ACCGAGAAAT TCGGCTTCCA CATTCACACC GAAACGCGCG AACAGCTCAA TCCCGGCCAG AAGGTCTGGT ATGCGGTGCG CCCGGAAAAG ACCCGCATCT CGCGCGAGAA ACCCGACGAG GCCGCCGTCA ATGCGGTGGA GGGCGAGCTT TGGGATATTG CCTATTTCGG CGACATGACG GTCTTTCACG TGCGTCTCGA CAATGGCCGC ACTGTCAAGG CAGCGAGCCT CAATGCCGTG CGCAAGACTG AAAACCCGCT CACCTATGAC GAGCGCGTCT GGGTTTCATT CGATATCGAT GCCGGTCTGG TGCTGACGGC CAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA
Details for BrabA.10014.f.A1.PW27651
PURIFICATION DATe: 3/22/2010
CONCENTRATION: 6.76mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 15
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMESFGSFRR KFAPWNDPAA KPFISFENVT KIFGDFTAVD NLSLNVFNRE FFALLGASGC GKTTLMRMLA GFEEPTSGRI TLDGQDMRGI PPYKRPVNMM FQSYALFPHM TVEKNIAFGL KQDGMAKSEI DARVAEMLKL VKLEQYGKRK PHQLSGGQRQ RVALARSVAK RPKVLLLDEP LGALDKKLRE ETQFELMDLQ VKLGMTFMVV THDQEEAMTM ADRIAVMDKG RIMQVATPAE VYEAPRSKFV ADFIGNVNII EGEVVKAANG EAEIATEKFG FHIHTETREQ LNPGQKVWYA VRPEKTRISR EKPDEAAVNA VEGELWDIAY FGDMTVFHVR LDNGRTVKAA SLNAVRKTEN PLTYDERVWV SFDIDAGLVL TA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaat cttttggcag ctttcgccgc aaattcgcgc cgtggaacga cccggcggcc aagcctttta tttctttcga gaatgtgacg aaaattttcg gtgatttcac tgccgtcgat aatctgtcgc tcaatgtttt caatcgggaa ttctttgcgc tgcttggtgc gtcgggttgc ggcaagacca cgctgatgcg tatgctggcg ggcttcgagg agccgacatc gggccgcatt acgctcgatg gtcaggatat gcgcggcatc ccgccctata agcgccccgt caacatgatg ttccagtcct atgcactgtt cccgcatatg acggtggaga agaatatcgc cttcggtctc aaacaggatg gcatggcgaa atcggagatc gatgcccgtg tcgctgaaat gctcaagctc gtgaagctgg agcaatatgg caagcgcaag ccacatcagt tatccggcgg ccagcgccag cgcgtggcgc tggcccgttc cgtcgccaag cgcccgaagg tgttgctgct cgacgagccg cttggtgcgc tcgacaaaaa gctgcgcgag gaaacccagt ttgaattgat ggatttgcag gtcaagcttg gcatgacctt catggttgtc acccacgatc aggaagaagc catgaccatg gccgaccgta tcgcggtcat ggacaagggt cgcatcatgc aggtggcaac gcctgccgaa gtctatgaag cgccgcgctc caaattcgtg gccgatttca tcggcaatgt gaatatcatc gaaggcgagg tggtgaaggc ggcaaacggc gaggcggaaa tcgccaccga gaaattcggc ttccacattc acaccgaaac gcgcgaacag ctcaatcccg gccagaaggt ctggtatgcg gtgcgcccgg aaaagacccg catctcgcgc gagaaacccg acgaggccgc cgtcaatgcg gtggagggcg agctttggga tattgcctat ttcggcgaca tgacggtctt tcacgtgcgt ctcgacaatg gccgcactgt caaggcagcg agcctcaatg ccgtgcgcaa gactgaaaac ccgctcacct atgacgagcg cgtctgggtt tcattcgata tcgatgccgg tctggtgctg acggcctaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMESFGSFRR KFAPWNDPAA KPFISFENVT KIFGDFTAVD NLSLNVFNRE FFALLGASGC GKTTLMRMLA GFEEPTSGRI TLDGQDMRGI PPYKRPVNMM FQSYALFPHM TVEKNIAFGL KQDGMAKSEI DARVAEMLKL VKLEQYGKRK PHQLSGGQRQ RVALARSVAK RPKVLLLDEP LGALDKKLRE ETQFELMDLQ VKLGMTFMVV THDQEEAMTM ADRIAVMDKG RIMQVATPAE VYEAPRSKFV ADFIGNVNII EGEVVKAANG EAEIATEKFG FHIHTETREQ LNPGQKVWYA VRPEKTRISR EKPDEAAVNA VEGELWDIAY FGDMTVFHVR LDNGRTVKAA SLNAVRKTEN PLTYDERVWV SFDIDAGLVL TA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAATCTTTT GGCAGCTTTC GCCGCAAATT CGCGCCGTGG AACGACCCGG CGGCCAAGCC TTTTATTTCT TTCGAGAATG TGACGAAAAT TTTCGGTGAT TTCACTGCCG TCGATAATCT GTCGCTCAAT GTTTTCAATC GGGAATTCTT TGCGCTGCTT GGTGCGTCGG GTTGCGGCAA GACCACGCTG ATGCGTATGC TGGCGGGCTT CGAGGAGCCG ACATCGGGCC GCATTACGCT CGATGGTCAG GATATGCGCG GCATCCCGCC CTATAAGCGC CCCGTCAACA TGATGTTCCA GTCCTATGCA CTGTTCCCGC ATATGACGGT GGAGAAGAAT ATCGCCTTCG GTCTCAAACA GGATGGCATG GCGAAATCGG AGATCGATGC CCGTGTCGCT GAAATGCTCA AGCTCGTGAA GCTGGAGCAA TATGGCAAGC GCAAGCCACA TCAGTTATCC GGCGGCCAGC GCCAGCGCGT GGCGCTGGCC CGTTCCGTCG CCAAGCGCCC GAAGGTGTTG CTGCTCGACG AGCCGCTTGG TGCGCTCGAC AAAAAGCTGC GCGAGGAAAC CCAGTTTGAA TTGATGGATT TGCAGGTCAA GCTTGGCATG ACCTTCATGG TTGTCACCCA CGATCAGGAA GAAGCCATGA CCATGGCCGA CCGTATCGCG GTCATGGACA AGGGTCGCAT CATGCAGGTG GCAACGCCTG CCGAAGTCTA TGAAGCGCCG CGCTCCAAAT TCGTGGCCGA TTTCATCGGC AATGTGAATA TCATCGAAGG CGAGGTGGTG AAGGCGGCAA ACGGCGAGGC GGAAATCGCC ACCGAGAAAT TCGGCTTCCA CATTCACACC GAAACGCGCG AACAGCTCAA TCCCGGCCAG AAGGTCTGGT ATGCGGTGCG CCCGGAAAAG ACCCGCATCT CGCGCGAGAA ACCCGACGAG GCCGCCGTCA ATGCGGTGGA GGGCGAGCTT TGGGATATTG CCTATTTCGG CGACATGACG GTCTTTCACG TGCGTCTCGA CAATGGCCGC ACTGTCAAGG CAGCGAGCCT CAATGCCGTG CGCAAGACTG AAAACCCGCT CACCTATGAC GAGCGCGTCT GGGTTTCATT CGATATCGAT GCCGGTCTGG TGCTGACGGC CAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA