SSGCID: Target Page

BrabA.10014.e

ATP/GTP-binding site motif A (P-loop):ABC transporter:AAA ATPase

CENTER ID:	BrabA.10014.e
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.10014.e.A1.GE27762	Full length( BrabA.10014.e )		1	227

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.879
RefSeq:	YP_414275.1
UniProt:	Q2YNH6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAAEIILRLE RIGRAYKEAD RELIILNDAD FTLRRGEMVA LVAPSGAGKS TLLHTAGLLE RPDSGDVVLD GRSCSKLSDD ERTAVRRNDV GFVYQFHHLL PEFSALENVM LPQMIRGLSR KAAAERAQQL LEYMKIGKRA SHRPAELSGG EQQRVAIARA VANAPLVLLA DEPTGNLDPT TSSYVFGALE ALVRQSGLAA LIATHNHELA RRMDRRVTLK DGRVVDL

NT Sequence

ATGGCGGCAG AAATCATCCT GCGGCTAGAG CGCATCGGCC GCGCCTATAA GGAAGCCGAC CGCGAGCTGA TTATCCTGAA CGATGCCGAT TTCACGCTTC GTCGCGGCGA GATGGTGGCG CTTGTTGCGC CATCGGGTGC AGGCAAGTCA ACGCTTCTGC ATACGGCCGG CCTTCTGGAG CGCCCTGACA GTGGTGATGT CGTTCTGGAC GGACGTTCCT GTAGCAAGCT TTCGGATGAT GAGCGCACCG CCGTGCGCCG CAACGATGTC GGCTTTGTCT ATCAGTTCCA TCACCTCCTG CCGGAGTTTT CCGCACTGGA AAATGTGATG CTGCCGCAAA TGATTCGCGG CCTTTCCAGA AAGGCGGCAG CAGAACGCGC GCAGCAGCTT CTTGAATATA TGAAGATCGG CAAACGCGCC TCGCATCGCC CGGCAGAGCT TTCCGGCGGC GAACAGCAGC GCGTCGCAAT CGCGCGCGCG GTGGCCAATG CGCCGCTCGT GCTTCTGGCC GACGAGCCGA CCGGCAATCT CGATCCGACG ACATCTTCCT ATGTTTTCGG TGCGCTGGAG GCGCTCGTGC GCCAGTCGGG CCTTGCTGCC CTGATTGCGA CCCACAACCA CGAACTGGCG CGCCGCATGG ACCGGCGGGT GACGCTGAAA GACGGTAGGG TCGTCGATCT T

Details for BrabA.10014.e.A1.GE27762

HARVESTED ON:	11/16/2009
SEQUENCED ON:	11/23/2009
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	97
PERCENT COVERAGE:	95

Validated AA Sequence

MGXXEXQTQG PGSMAAEIIL RLERIGRAYK EADRELIIXN DADFTXRRGE MVALVAPSGA GKSTLLHTAG LLERPDSGDV VLDGRSCSKL SDDERTAVRR NDVGFVYQFH HLLPEFSALE NVMLPQMIRG LSRKAAAERA QQLLEYMKIG KRASHRPAEL SGGEQQRVAI ARAVANAPLV LLADEPTGNL DPTTSSYVFG ALEALVRQSG LAALIATHNH ELARRMDRRV TLKDGRVV

Validated NT Sequence

cgacgaccct accgtctttc agcgtcaccc gccggtccat gcggcgcgcc agttcgtggt tgtgggtcgc aatcagggca gcaaggcccg actggcgcac gagcgcctcc agcgcaccga aaacatagga agatgtcgtc ggatcgagat tgccggtcgg ctcgtcggcc agaagcacga gcggcgcatt ggccaccgcg cgcgcgattg cgacgcgctg ctgttcgccg ccggaaagct ctgccgggcg atgcgaggcg cgtttgccga tcttcatata ttcaagaagc tgctgcgcgc gttctgctgc cgcctttctg gaaaggccgc gaatcatttg cggcagcatc acattttcca gtgcggaaaa ctccggcagg aggtgatgga actgatagac aaagccgaca tcgttacggc gcacggcggt gcgctcatca tccgaaagct tgctacagga acgtccgtcc agaacgacat caccactgtc agggcgctcc agaaggccgg ccgtatgcag aagcgttgac ttgcctgcac ccgatggcgc aacaagcgcc accatctcgc cgcgacgaan cgtgaaatcg gcatcgttca ngataatcag ctcgcggtcg gcttccttat aggcgcggcc gatgcgctct agccgcagga tgatttctgc cgccatcgaa ccaggaccct gggtctganc ttccangnna cccatatgat ggtgatggtg atga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAAEIILRL ERIGRAYKEA DRELIILNDA DFTLRRGEMV ALVAPSGAGK STLLHTAGLL ERPDSGDVVL DGRSCSKLSD DERTAVRRND VGFVYQFHHL LPEFSALENV MLPQMIRGLS RKAAAERAQQ LLEYMKIGKR ASHRPAELSG GEQQRVAIAR AVANAPLVLL ADEPTGNLDP TTSSYVFGAL EALVRQSGLA ALIATHNHEL ARRMDRRVTL KDGRVVDL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggcagaa atcatcctgc ggctagagcg catcggccgc gcctataagg aagccgaccg cgagctgatt atcctgaacg atgccgattt cacgcttcgt cgcggcgaga tggtggcgct tgttgcgcca tcgggtgcag gcaagtcaac gcttctgcat acggccggcc ttctggagcg ccctgacagt ggtgatgtcg ttctggacgg acgttcctgt agcaagcttt cggatgatga gcgcaccgcc gtgcgccgca acgatgtcgg ctttgtctat cagttccatc acctcctgcc ggagttttcc gcactggaaa atgtgatgct gccgcaaatg attcgcggcc tttccagaaa ggcggcagca gaacgcgcgc agcagcttct tgaatatatg aagatcggca aacgcgcctc gcatcgcccg gcagagcttt ccggcggcga acagcagcgc gtcgcaatcg cgcgcgcggt ggccaatgcg ccgctcgtgc ttctggccga cgagccgacc ggcaatctcg atccgacgac atcttcctat gttttcggtg cgctggaggc gctcgtgcgc cagtcgggcc ttgctgccct gattgcgacc cacaaccacg aactggcgcg ccgcatggac cggcgggtga cgctgaaaga cggtagggtc gtcgatctta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a