SSGCID: Target Page

BrabA.10014.d

ATP/GTP-binding site motif A (P-loop):ABC transporter:AAA ATPase

CENTER ID:	BrabA.10014.d
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.10014.d.A1.GE27761	Full length( BrabA.10014.d )		1	249

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.803
RefSeq:	YP_414202.1
UniProt:	Q2YN82

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQVSKTEVAI EITNMHKWYG EFHVLRDINL KVMRGERIVV AGPSGSGKST MIRCINRLEE HQKGKIVVDG IELTNDLKKI DEVRREVGMV FQHFNLFPHL TILENCTLAP IWVRKMPKKQ AEEIAMHYLE RVKIPEQANK YPGQLSGGQQ QRVAIARALC MNPKVMLFDE PTSALDPEMV KEVLDTMVSL AAEGMTMICV THEMGFARQV ANRVIFMDQG QIVEQNSPDE FFDNPQHERT RLFLSQILH

NT Sequence

ATGCAGGTTT CCAAAACCGA AGTCGCCATC GAGATCACCA ATATGCACAA GTGGTATGGT GAGTTCCACG TTCTGCGTGA CATCAACCTC AAGGTTATGC GTGGTGAGCG TATCGTCGTG GCCGGACCGT CCGGTTCTGG CAAATCGACC ATGATCCGCT GCATCAACCG TCTGGAAGAA CATCAGAAAG GCAAGATCGT CGTCGATGGT ATCGAGCTTA CCAACGATCT GAAGAAAATC GATGAAGTGC GCCGCGAAGT CGGCATGGTG TTCCAGCACT TCAACCTCTT CCCGCATCTA ACCATTCTGG AAAACTGCAC GCTTGCGCCG ATCTGGGTGC GCAAGATGCC GAAAAAGCAG GCGGAAGAAA TCGCCATGCA TTATCTGGAG CGTGTGAAGA TCCCGGAACA GGCCAACAAA TATCCGGGCC AGCTTTCCGG CGGCCAGCAG CAGCGTGTGG CGATTGCGCG CGCGCTTTGC ATGAACCCGA AAGTCATGCT GTTCGATGAG CCGACCTCGG CGCTCGATCC GGAAATGGTC AAGGAAGTGC TGGATACGAT GGTGAGCCTT GCTGCCGAAG GCATGACGAT GATCTGCGTG ACGCACGAAA TGGGCTTTGC CCGTCAGGTT GCCAACCGCG TCATCTTCAT GGATCAGGGC CAGATCGTCG AACAGAATTC GCCAGACGAA TTCTTCGATA ATCCGCAGCA TGAACGCACC AGGCTGTTCC TGAGCCAGAT CCTGCAT

Details for BrabA.10014.d.A1.GE27761

HARVESTED ON:	11/16/2009
SEQUENCED ON:	11/23/2009
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	87

Validated AA Sequence

MHXWYGEFHV LXDXXLKVMR GERIVVAGPS GSGXSTMIRC INRLEEHQKG KIVVDGIELT NDLKKIDEVR REVGMVFQHF NLFPHLTILE NCTLAPIWVR KMPKKQAEEI AMHYLERVKI PEQANKYPGQ LSGGQQQRVA IARALCMNPK VMLFDEPTSA LDPEMVKEVL DTMVSLAAEG MTMICVTHEM GFARQVANRV IFMDQGQIVE QNSPDEFFDN PQHERTRLFL SQILH

Validated NT Sequence

ttattaatgc aggatctggc tcaggaacag cctggtgcgt tcatgctgcg gattatcgaa gaattcgtct ggcgaattct gttcgacgat ctggccctga tccatgaaga tgacgcggtt ggcaacctga cgggcaaagc ccatttcgtg cgtcacgcag atcatcgtca tgccttcggc agcaaggctc accatcgtat ccagcacttc cttgaccatt tccggatcga gcgccgaggt cggctcatcg aacagcatga ctttcgggtt catgcaaagc gcgcgcgcaa tcgccacacg ctgctgctgg ccgccggaaa gctggcccgg atatttgttg gcctgttccg ggatcttcac acgctccaga taatgcatgg cgatttcttc cgcctgcttt ttcggcatct tgcgcaccca gatcggcgca agcgtgcagt tttccagaat ggttagatgc gggaagaggt tgaagtgctg gaacaccatg ccgacttcgc ggcgcacttc atcgattttc ttcagatcgt tggtaagctc gataccatcg acgacgatct tgcctttctg atgttcttcc agacggttga tgcagcggat catggtcgan ttgccagaac cggacggtcc ggccacgacg atacgctcac cacgcataac cttgaggntg angtcacnca gaacgtggaa ctcaccatac cactngtgca tattggtgat ctcgatggcn antnc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMQVSKTEVA IEITNMHKWY GEFHVLRDIN LKVMRGERIV VAGPSGSGKS TMIRCINRLE EHQKGKIVVD GIELTNDLKK IDEVRREVGM VFQHFNLFPH LTILENCTLA PIWVRKMPKK QAEEIAMHYL ERVKIPEQAN KYPGQLSGGQ QQRVAIARAL CMNPKVMLFD EPTSALDPEM VKEVLDTMVS LAAEGMTMIC VTHEMGFARQ VANRVIFMDQ GQIVEQNSPD EFFDNPQHER TRLFLSQILH

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaggtttcc aaaaccgaag tcgccatcga gatcaccaat atgcacaagt ggtatggtga gttccacgtt ctgcgtgaca tcaacctcaa ggttatgcgt ggtgagcgta tcgtcgtggc cggaccgtcc ggttctggca aatcgaccat gatccgctgc atcaaccgtc tggaagaaca tcagaaaggc aagatcgtcg tcgatggtat cgagcttacc aacgatctga agaaaatcga tgaagtgcgc cgcgaagtcg gcatggtgtt ccagcacttc aacctcttcc cgcatctaac cattctggaa aactgcacgc ttgcgccgat ctgggtgcgc aagatgccga aaaagcaggc ggaagaaatc gccatgcatt atctggagcg tgtgaagatc ccggaacagg ccaacaaata tccgggccag ctttccggcg gccagcagca gcgtgtggcg attgcgcgcg cgctttgcat gaacccgaaa gtcatgctgt tcgatgagcc gacctcggcg ctcgatccgg aaatggtcaa ggaagtgctg gatacgatgg tgagccttgc tgccgaaggc atgacgatga tctgcgtgac gcacgaaatg ggctttgccc gtcaggttgc caaccgcgtc atcttcatgg atcagggcca gatcgtcgaa cagaattcgc cagacgaatt cttcgataat ccgcagcatg aacgcaccag gctgttcctg agccagatcc tgcataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa