BrabA.10014.c

ATP/GTP-binding site motif A (P-loop):ABC transporter:AAA ATPase

CENTER ID:	BrabA.10014.c
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.10014.c.A1.GE27760	Full length( BrabA.10014.c )		1	312

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_135381
PATRIC ID:	fig\|359391.4.peg.235
RefSeq:	YP_413705.1
UniProt:	Q2YP78

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIEFDRITKN YNGRAAVDEV SFTIEPHSIA AIVGTSGSGK TTLLRMVNRL EEPTSGEIRI DGQSILDEPG HELRRRIGYV IQQHGLFPHR TVGENIATVP ALLGWPKQRI EARVDELLEL FQLEPGAYRD RYPHELSGGQ QQRVGVARAL AAGPNILLMD EPFGALDPVI RAKAQEDLKA IQRRFGTTIL LVTHDMEEAI SLGDRIAVMD EGKLLQFGPP AEILARPATE FVERLVGTGE RPFRLLSLTD LKTVLQPGDA SGEPLPVTAS QRDALAELLW SGRRALPVLD EDGRSLGRVT LEKLLHQAER PA

NT Sequence

ATGATCGAGT TCGACCGGAT CACCAAGAAT TACAATGGCC GGGCGGCGGT GGACGAGGTG AGCTTCACCA TCGAACCGCA TAGTATCGCG GCGATTGTGG GCACGTCCGG CTCCGGCAAG ACGACACTGC TACGCATGGT CAACCGGCTG GAGGAACCGA CCTCCGGCGA AATCCGCATT GACGGGCAGA GCATTCTGGA TGAGCCGGGC CATGAATTAC GGCGGCGGAT CGGCTATGTG ATCCAGCAAC ACGGGCTTTT TCCACATCGA ACGGTCGGCG AAAATATCGC GACCGTTCCC GCACTCCTCG GCTGGCCGAA ACAGCGCATT GAGGCGCGCG TGGATGAATT GCTGGAGCTG TTCCAGCTTG AGCCGGGCGC CTATCGCGAC CGCTATCCGC ACGAACTTTC CGGCGGGCAG CAACAGCGCG TCGGCGTGGC GCGGGCCCTT GCCGCCGGAC CGAACATCCT CCTGATGGAC GAACCTTTCG GCGCGCTTGA CCCGGTGATC CGCGCCAAGG CGCAGGAAGA CCTCAAGGCG ATCCAGCGGC GCTTCGGCAC GACCATCCTT CTCGTCACGC ATGATATGGA GGAGGCGATC TCGCTTGGCG ACCGCATTGC GGTGATGGAT GAGGGCAAGC TTTTGCAATT CGGCCCGCCC GCCGAAATTC TGGCCCGCCC CGCGACCGAA TTCGTGGAAC GGCTGGTCGG AACCGGAGAG CGGCCGTTCC GCCTTCTCTC GCTCACCGAT TTGAAAACCG TGTTGCAACC GGGCGATGCC AGCGGCGAAC CCCTGCCCGT CACAGCCTCG CAGCGCGATG CGCTGGCGGA GCTTCTCTGG TCGGGCCGAA GGGCGCTGCC GGTGCTCGAT GAAGACGGCC GATCGCTTGG CCGCGTGACA TTGGAAAAGC TACTGCATCA GGCAGAGCGC CCGGCA

Details for BrabA.10014.c.A1.GE27760

HARVESTED ON:	11/16/2009
SEQUENCED ON:	11/23/2009
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMIEFDRITK NYNGRAAVDE VSFTIEPHSI AAIVGTSGSG KTTLLRMVNR LEEPTSGEIR IDGQSILDEP GHELRRRIGY VIQQHGLFPH RTVGENIATV PALLGWPKQR IEARVDELLE LFQLEPGAYR DRYPHELSGG QQQRVGVARA LAAGPNILLM DEPFGALDPV IRAKAQEDLK AIQRRFGTTI LLVTHDMEEA ISLGDRIAVM DEGKLLQFGP PAEILARPAT EFVERLVGTG ERPFRLLSLT DLKTVLQPGD ASGEPLPVTA SQRDALAELL WSGRRALPVL DEDGRSLGRV TLEKLLHQAE RPA

Validated NT Sequence

tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgatcga gttcgaccgg atcaccaaga attacaatgg ccgggcggcg gtggacgagg tgagcttcac catcgaaccg catagtatcg cggcgattgt gggcacgtcc ggctccggca agacgacact gctacgcatg gtcaaccggc tggaggaacc gacctccggc gaaatccgca ttgacgggca gagcattctg gatgagccgg gccatgaatt acggcggcgg atcggctatg tgatccagca acacgggctt tttccacatc gaacggtcgg cgaaaatatc gcgaccgttc ccgcactcct cggctggccg aaacagcgca ttgaggcgcg cgtggatgaa ttgctggagc tgttccagct tgagccgggc gcctatcgcg accgctatcc gcacgaactt tccggcgggc agcaacagcg cgtcggcgtg gcgcgggccc ttgccgccgg accgaacatc ctcctgatgg acgaaccttt cggcgcgctt gacccggtga tccgcgccaa ggcgcaggaa gacctcaagg cgatccagcg gcgcttcggc acgaccatcc ttctcgtcac gcatgatatg gaggaggcga tctcgcttgg cgaccgcatt gcggtgatgg atgagggcaa gcttttgcaa ttcggcccgc ccgccgaaat tctggcccgc cccgcgaccg aattcgtgga acggctggtc ggaaccggag agcggccgtt ccgccttctc tcgctcaccg atttgaaaac cgtgttgcaa ccgggcgatg ccagcggcga acccctgccc gtcacagcct cgcagcgcga tgcgctggcg gagcttctct ggtcgggccg aagggcgctg ccggtgctcg atgaagacgg ccgatcgctt ggccgcgtga cattggaaaa gctactgcat caggcagagc gcccggcata ataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatcgagttc gaccggatca ccaagaatta caatggccgg gcggcggtgg acgaggtgag cttcaccatc gaaccgcata gtatcgcggc gattgtgggc acgtccggct ccggcaagac gacactgcta cgcatggtca accggctgga ggaaccgacc tccggcgaaa tccgcattga cgggcagagc attctggatg agccgggcca tgaattacgg cggcggatcg gctatgtgat ccagcaacac gggctttttc cacatcgaac ggtcggcgaa aatatcgcga ccgttcccgc actcctcggc tggccgaaac agcgcattga ggcgcgcgtg gatgaattgc tggagctgtt ccagcttgag ccgggcgcct atcgcgaccg ctatccgcac gaactttccg gcgggcagca acagcgcgtc ggcgtggcgc gggcccttgc cgccggaccg aacatcctcc tgatggacga acctttcggc gcgcttgacc cggtgatccg cgccaaggcg caggaagacc tcaaggcgat ccagcggcgc ttcggcacga ccatccttct cgtcacgcat gatatggagg aggcgatctc gcttggcgac cgcattgcgg tgatggatga gggcaagctt ttgcaattcg gcccgcccgc cgaaattctg gcccgccccg cgaccgaatt cgtggaacgg ctggtcggaa ccggagagcg gccgttccgc cttctctcgc tcaccgattt gaaaaccgtg ttgcaaccgg gcgatgccag cggcgaaccc ctgcccgtca cagcctcgca gcgcgatgcg ctggcggagc ttctctggtc gggccgaagg gcgctgccgg tgctcgatga agacggccga tcgcttggcc gcgtgacatt ggaaaagcta ctgcatcagg cagagcgccc ggcaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa