BrabA.10014.b

ATP/GTP-binding site motif A (P-loop):ABC transporter:AAA ATPase

CENTER ID: BrabA.10014.b
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.10014.b.MB1.GE30434 Full length( BrabA.10014.b ) 1 359
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.10014.b.MB1.PW30567 Full length( BrabA.10014.b ) 1 359
BrabA.10014.b.MB1.PW30569 Full length( BrabA.10014.b ) 1 359

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.114
RefSeq: YP_413603.1
UniProt: Q2YNV8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEVRVAGVRK EFARFPALHN VSLTIQSGEL IALLGPSGSG KTTLLRLIAG LETPTEGMIF FGDEDASQKS VQERNVGFVF QHYALFRHMT VADNIGFGLK VRPGGTRPST AEIRRRALEL LDLVQLSGLE KRYPAQLSGG QRQRVALARA MAIEPRVLLL DEPFGALDAQ VRKELRRWLR EIHDKTGHTT VFVTHDQDEA LELADRVVVM SQGRIEQVGT PDEVYDKPNS PFVYGFIGES STLPVRVENG EVWLADRNIG LGAENMPDGD AQLYFRPHDV ELLDGCGGCI AGTVIASRRS GGKRRVELEV GGARERIEIE IPAEHPAAEK SRIAFRPRYW TLFRAGDSEL PAPKAAATT
NT Sequence
ATGGAAGTTC GTGTTGCCGG CGTGCGTAAG GAGTTTGCTC GCTTTCCGGC CTTGCATAAT GTGTCGCTGA CCATCCAGTC CGGCGAATTG ATTGCCCTGC TTGGACCATC GGGTTCGGGC AAGACCACGC TTTTGCGCCT GATTGCGGGG CTGGAAACGC CAACCGAAGG CATGATCTTC TTCGGGGATG AAGATGCCTC GCAAAAGAGC GTGCAGGAGC GTAATGTCGG TTTTGTCTTC CAGCATTATG CGCTGTTCCG CCATATGACG GTGGCGGATA ATATCGGTTT CGGGTTGAAG GTTCGCCCCG GTGGGACGCG CCCATCCACG GCGGAAATTC GCCGCCGTGC GCTGGAGCTT CTTGATCTCG TGCAGTTGAG CGGGCTGGAA AAACGCTATC CGGCGCAGCT TTCCGGCGGC CAGCGCCAGC GCGTGGCGCT GGCCCGCGCC ATGGCGATCG AACCGCGTGT GCTTTTGCTG GATGAGCCTT TTGGTGCACT GGATGCGCAG GTGCGCAAGG AATTGCGCCG CTGGCTGCGC GAAATCCACG ACAAGACCGG CCATACCACG GTTTTCGTGA CCCATGACCA GGATGAGGCG CTGGAACTTG CCGACCGCGT GGTGGTGATG AGCCAGGGCC GCATCGAGCA GGTCGGCACG CCCGACGAGG TCTATGACAA GCCCAACTCT CCTTTCGTGT ACGGTTTCAT CGGCGAGTCC AGCACCCTGC CGGTGCGCGT GGAAAACGGT GAGGTCTGGC TGGCGGATCG CAATATCGGC CTTGGTGCGG AAAATATGCC GGATGGTGAC GCGCAGCTCT ATTTCCGCCC GCATGATGTC GAGTTGCTCG ACGGTTGCGG CGGCTGTATC GCGGGAACCG TGATTGCAAG CCGCCGTTCG GGTGGCAAGC GGCGCGTGGA ACTGGAAGTG GGGGGCGCCC GCGAGCGCAT CGAGATAGAA ATTCCGGCCG AGCATCCGGC GGCAGAGAAA AGCCGCATTG CCTTTCGCCC GCGCTACTGG ACGCTCTTTC GCGCAGGCGA TAGCGAACTT CCGGCGCCGA AGGCTGCGGC AACAACT
Details for BrabA.10014.b.MB1.GE30434
HARVESTED ON: 5/11/2010
SEQUENCED ON: 10/7/2010
EXPECTED MW: 84kDa
OBSERVED MW: 82kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 23
PERCENT COVERAGE: 34
Validated AA Sequence
MRAEIELRVT IRHIFRTKAD IAIRQPDLTV FHAHRQGAGL ADETVHERRV GLVIDLVGRA DLLDAALAHH HHAVGKFQRL ILVMGHENRG MAGLVVDFAQ PAAQFLAHLR IQCTKRLIQQ KHTRFDRHGA GQRHALALAA GKLRRIAFFQ PAQLHEIKKL QRTAANFRRG WARPTGANLQ PETDIIRHRH MAEQRIMLED KTDITLLHAL LRGIFIPEED HAFGWRFQPR NQAQKRGLAR TRWSKQGNQF AGLDGQRHIM QGRKAS
Validated NT Sequence
ctcgatgcgc tcgcgggcgc cccccacttc cagttccacg cgccgcttgc cacccgaacg gcggcttgca atcacggttc ccgcgataca gccgccgcaa ccgtcgagca actcgacatc atgcgggcgg aaatagagct gcgcgtcacc atccggcata ttttccgcac caaggccgat attgcgatcc gccagccaga cctcaccgtt ttccacgcgc accggcaggg tgctggactc gccgatgaaa ccgtacacga aaggagagtt gggcttgtca tagacctcgt cgggcgtgcc gacctgctcg atgcggccct ggctcatcac caccacgcgg tcggcaagtt ccagcgcctc atcctggtca tgggtcacga aaaccgtggt atggccggtc ttgtcgtgga tttcgcgcag ccagcggcgc aattccttgc gcacctgcgc atccagtgca ccaaaaggct catccagcaa aagcacacgc ggttcgatcg ccatggcgcg ggccagcgcc acgcgctggc gctggccgcc ggaaagctgc gccggatagc gtttttccag cccgctcaac tgcacgagat caagaagctc cagcgcacgg cggcgaattt ccgccgtgga tgggcgcgtc ccaccggggc gaaccttcaa cccgaaaccg atattatccg ccaccgtcat atggcggaac agcgcataat gctggaagac aaaaccgaca ttacgctcct gcacgctctt ttgcgaggca tcttcatccc cgaagaagat catgccttcg gttggcgttt ccagccccgc aatcaggcgc aaaagcgtgg tcttgcccga acccgatggt ccaagcaggg caatcaattc gccggactgg atggtcagcg acacattatg caaggccgga aagcgagcaa
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MEVRVAGVRK EFARFPALHN VSLTIQSGEL IALLGPSGSG KTTLLRLIAG LETPTEGMIF FGDEDASQKS VQERNVGFVF QHYALFRHMT VADNIGFGLK VRPGGTRPST AEIRRRALEL LDLVQLSGLE KRYPAQLSGG QRQRVALARA MAIEPRVLLL DEPFGALDAQ VRKELRRWLR EIHDKTGHTT VFVTHDQDEA LELADRVVVM SQGRIEQVGT PDEVYDKPNS PFVYGFIGES STLPVRVENG EVWLADRNIG LGAENMPDGD AQLYFRPHDV ELLDGCGGCI AGTVIASRRS GGKRRVELEV GGARERIEIE IPAEHPAAEK SRIAFRPRYW TLFRAGDSEL PAPKAAATT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatgga agttcgtgtt gccggcgtgc gtaaggagtt tgctcgcttt ccggccttgc ataatgtgtc gctgaccatc cagtccggcg aattgattgc cctgcttgga ccatcgggtt cgggcaagac cacgcttttg cgcctgattg cggggctgga aacgccaacc gaaggcatga tcttcttcgg ggatgaagat gcctcgcaaa agagcgtgca ggagcgtaat gtcggttttg tcttccagca ttatgcgctg ttccgccata tgacggtggc ggataatatc ggtttcgggt tgaaggttcg ccccggtggg acgcgcccat ccacggcgga aattcgccgc cgtgcgctgg agcttcttga tctcgtgcag ttgagcgggc tggaaaaacg ctatccggcg cagctttccg gcggccagcg ccagcgcgtg gcgctggccc gcgccatggc gatcgaaccg cgtgtgcttt tgctggatga gccttttggt gcactggatg cgcaggtgcg caaggaattg cgccgctggc tgcgcgaaat ccacgacaag accggccata ccacggtttt cgtgacccat gaccaggatg aggcgctgga acttgccgac cgcgtggtgg tgatgagcca gggccgcatc gagcaggtcg gcacgcccga cgaggtctat gacaagccca actctccttt cgtgtacggt ttcatcggcg agtccagcac cctgccggtg cgcgtggaaa acggtgaggt ctggctggcg gatcgcaata tcggccttgg tgcggaaaat atgccggatg gtgacgcgca gctctatttc cgcccgcatg atgtcgagtt gctcgacggt tgcggcggct gtatcgcggg aaccgtgatt gcaagccgcc gttcgggtgg caagcggcgc gtggaactgg aagtgggggg cgcccgcgag cgcatcgaga tagaaattcc ggccgagcat ccggcggcag agaaaagccg cattgccttt cgcccgcgct actggacgct ctttcgcgca ggcgatagcg aacttccggc gccgaaggct gcggcaacaa cttaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BrabA.10014.b.MB1.PW30567
PURIFICATION DATe: 9/20/2010
CONCENTRATION: 5.67mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 23
PERCENT COVERAGE: 34
Protocol Notes
notes unavailable
Validated AA Sequence
MRAEIELRVT IRHIFRTKAD IAIRQPDLTV FHAHRQGAGL ADETVHERRV GLVIDLVGRA DLLDAALAHH HHAVGKFQRL ILVMGHENRG MAGLVVDFAQ PAAQFLAHLR IQCTKRLIQQ KHTRFDRHGA GQRHALALAA GKLRRIAFFQ PAQLHEIKKL QRTAANFRRG WARPTGANLQ PETDIIRHRH MAEQRIMLED KTDITLLHAL LRGIFIPEED HAFGWRFQPR NQAQKRGLAR TRWSKQGNQF AGLDGQRHIM QGRKAS
Validated NT Sequence
ctcgatgcgc tcgcgggcgc cccccacttc cagttccacg cgccgcttgc cacccgaacg gcggcttgca atcacggttc ccgcgataca gccgccgcaa ccgtcgagca actcgacatc atgcgggcgg aaatagagct gcgcgtcacc atccggcata ttttccgcac caaggccgat attgcgatcc gccagccaga cctcaccgtt ttccacgcgc accggcaggg tgctggactc gccgatgaaa ccgtacacga aaggagagtt gggcttgtca tagacctcgt cgggcgtgcc gacctgctcg atgcggccct ggctcatcac caccacgcgg tcggcaagtt ccagcgcctc atcctggtca tgggtcacga aaaccgtggt atggccggtc ttgtcgtgga tttcgcgcag ccagcggcgc aattccttgc gcacctgcgc atccagtgca ccaaaaggct catccagcaa aagcacacgc ggttcgatcg ccatggcgcg ggccagcgcc acgcgctggc gctggccgcc ggaaagctgc gccggatagc gtttttccag cccgctcaac tgcacgagat caagaagctc cagcgcacgg cggcgaattt ccgccgtgga tgggcgcgtc ccaccggggc gaaccttcaa cccgaaaccg atattatccg ccaccgtcat atggcggaac agcgcataat gctggaagac aaaaccgaca ttacgctcct gcacgctctt ttgcgaggca tcttcatccc cgaagaagat catgccttcg gttggcgttt ccagccccgc aatcaggcgc aaaagcgtgg tcttgcccga acccgatggt ccaagcaggg caatcaattc gccggactgg atggtcagcg acacattatg caaggccgga aagcgagcaa
Expressed Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MEVRVAGVRK EFARFPALHN VSLTIQSGEL IALLGPSGSG KTTLLRLIAG LETPTEGMIF FGDEDASQKS VQERNVGFVF QHYALFRHMT VADNIGFGLK VRPGGTRPST AEIRRRALEL LDLVQLSGLE KRYPAQLSGG QRQRVALARA MAIEPRVLLL DEPFGALDAQ VRKELRRWLR EIHDKTGHTT VFVTHDQDEA LELADRVVVM SQGRIEQVGT PDEVYDKPNS PFVYGFIGES STLPVRVENG EVWLADRNIG LGAENMPDGD AQLYFRPHDV ELLDGCGGCI AGTVIASRRS GGKRRVELEV GGARERIEIE IPAEHPAAEK SRIAFRPRYW TLFRAGDSEL PAPKAAATT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCACA TGAAAATCGA AGAAGGTAAA CTGGTAATCT GGATTAACGG CGATAAAGGC TATAACGGTC TCGCTGAAGT CGGTAAGAAA TTCGAGAAAG ATACCGGAAT TAAAGTCACC GTTGAGCATC CGGATAAACT GGAAGAGAAA TTCCCACAGG TTGCGGCAAC TGGCGATGGC CCTGACATTA TCTTCTGGGC ACACGACCGC TTTGGTGGCT ACGCTCAATC TGGCCTGTTG GCTGAAATCA CCCCGGACAA AGCGTTCCAG GACAAGCTGT ATCCGTTTAC CTGGGATGCC GTACGTTACA ACGGCAAGCT GATTGCTTAC CCGATCGCTG TTGAAGCGTT ATCGCTGATT TATAACAAAG ATCTGCTGCC GAACCCGCCA AAAACCTGGG AAGAGATCCC GGCGCTGGAT AAAGAACTGA AAGCGAAAGG TAAGAGCGCG CTGATGTTCA ACCTGCAAGA ACCGTACTTC ACCTGGCCGC TGATTGCTGC TGACGGGGGT TATGCGTTCA AGTATGAAAA CGGCAAGTAC GACATTAAAG ACGTGGGCGT GGATAACGCT GGCGCGAAAG CGGGTCTGAC CTTCCTGGTT GACCTGATTA AAAACAAACA CATGAATGCA GACACCGATT ACTCCATCGC AGAAGCTGCC TTTAATAAAG GCGAAACAGC GATGACCATC AACGGCCCGT GGGCATGGTC CAACATCGAC ACCAGCAAAG TGAATTATGG TGTAACGGTA CTGCCGACCT TCAAGGGTCA ACCATCCAAA CCGTTCGTTG GCGTGCTGAG CGCAGGTATT AACGCCGCCA GTCCGAACAA AGAGCTGGCA AAAGAGTTCC TCGAAAACTA TCTGCTGACT GATGAAGGTC TGGAAGCGGT TAATAAAGAC AAACCGCTGG GTGCCGTAGC GCTGAAGTCT TACGAGGAAG AGTTGGCGAA AGATCCACGT ATTGCCGCCA CTATGGAAAA CGCCCAGAAA GGTGAAATCA TGCCGAACAT CCCGCAGATG TCCGCTTTCT GGTATGCCGT GCGTACTGCG GTGATCAACG CCGCCAGCGG TCGTCAGACT GTCGATGAAG CCCTGAAAGA CGCGCAGACT AATTCGAGCT CGAACAACAA CAACAATAAC AATAACAACA ACCTCGGGAT CGAGGGAAGG ATTTCAGAAG GTACCCTGGA AGCTCAGACC CAGGGTCCTG GTTCGATGGA AGTTCGTGTT GCCGGCGTGC GTAAGGAGTT TGCTCGCTTT CCGGCCTTGC ATAATGTGTC GCTGACCATC CAGTCCGGCG AATTGATTGC CCTGCTTGGA CCATCGGGTT CGGGCAAGAC CACGCTTTTG CGCCTGATTG CGGGGCTGGA AACGCCAACC GAAGGCATGA TCTTCTTCGG GGATGAAGAT GCCTCGCAAA AGAGCGTGCA GGAGCGTAAT GTCGGTTTTG TCTTCCAGCA TTATGCGCTG TTCCGCCATA TGACGGTGGC GGATAATATC GGTTTCGGGT TGAAGGTTCG CCCCGGTGGG ACGCGCCCAT CCACGGCGGA AATTCGCCGC CGTGCGCTGG AGCTTCTTGA TCTCGTGCAG TTGAGCGGGC TGGAAAAACG CTATCCGGCG CAGCTTTCCG GCGGCCAGCG CCAGCGCGTG GCGCTGGCCC GCGCCATGGC GATCGAACCG CGTGTGCTTT TGCTGGATGA GCCTTTTGGT GCACTGGATG CGCAGGTGCG CAAGGAATTG CGCCGCTGGC TGCGCGAAAT CCACGACAAG ACCGGCCATA CCACGGTTTT CGTGACCCAT GACCAGGATG AGGCGCTGGA ACTTGCCGAC CGCGTGGTGG TGATGAGCCA GGGCCGCATC GAGCAGGTCG GCACGCCCGA CGAGGTCTAT GACAAGCCCA ACTCTCCTTT CGTGTACGGT TTCATCGGCG AGTCCAGCAC CCTGCCGGTG CGCGTGGAAA ACGGTGAGGT CTGGCTGGCG GATCGCAATA TCGGCCTTGG TGCGGAAAAT ATGCCGGATG GTGACGCGCA GCTCTATTTC CGCCCGCATG ATGTCGAGTT GCTCGACGGT TGCGGCGGCT GTATCGCGGG AACCGTGATT GCAAGCCGCC GTTCGGGTGG CAAGCGGCGC GTGGAACTGG AAGTGGGGGG CGCCCGCGAG CGCATCGAGA TAGAAATTCC GGCCGAGCAT CCGGCGGCAG AGAAAAGCCG CATTGCCTTT CGCCCGCGCT ACTGGACGCT CTTTCGCGCA GGCGATAGCG AACTTCCGGC GCCGAAGGCT GCGGCAACAA CTTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA
Details for BrabA.10014.b.MB1.PW30569
PURIFICATION DATe: 9/20/2010
CONCENTRATION: 23.65mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 23
PERCENT COVERAGE: 34
Protocol Notes
notes unavailable
Validated AA Sequence
MRAEIELRVT IRHIFRTKAD IAIRQPDLTV FHAHRQGAGL ADETVHERRV GLVIDLVGRA DLLDAALAHH HHAVGKFQRL ILVMGHENRG MAGLVVDFAQ PAAQFLAHLR IQCTKRLIQQ KHTRFDRHGA GQRHALALAA GKLRRIAFFQ PAQLHEIKKL QRTAANFRRG WARPTGANLQ PETDIIRHRH MAEQRIMLED KTDITLLHAL LRGIFIPEED HAFGWRFQPR NQAQKRGLAR TRWSKQGNQF AGLDGQRHIM QGRKAS
Validated NT Sequence
ctcgatgcgc tcgcgggcgc cccccacttc cagttccacg cgccgcttgc cacccgaacg gcggcttgca atcacggttc ccgcgataca gccgccgcaa ccgtcgagca actcgacatc atgcgggcgg aaatagagct gcgcgtcacc atccggcata ttttccgcac caaggccgat attgcgatcc gccagccaga cctcaccgtt ttccacgcgc accggcaggg tgctggactc gccgatgaaa ccgtacacga aaggagagtt gggcttgtca tagacctcgt cgggcgtgcc gacctgctcg atgcggccct ggctcatcac caccacgcgg tcggcaagtt ccagcgcctc atcctggtca tgggtcacga aaaccgtggt atggccggtc ttgtcgtgga tttcgcgcag ccagcggcgc aattccttgc gcacctgcgc atccagtgca ccaaaaggct catccagcaa aagcacacgc ggttcgatcg ccatggcgcg ggccagcgcc acgcgctggc gctggccgcc ggaaagctgc gccggatagc gtttttccag cccgctcaac tgcacgagat caagaagctc cagcgcacgg cggcgaattt ccgccgtgga tgggcgcgtc ccaccggggc gaaccttcaa cccgaaaccg atattatccg ccaccgtcat atggcggaac agcgcataat gctggaagac aaaaccgaca ttacgctcct gcacgctctt ttgcgaggca tcttcatccc cgaagaagat catgccttcg gttggcgttt ccagccccgc aatcaggcgc aaaagcgtgg tcttgcccga acccgatggt ccaagcaggg caatcaattc gccggactgg atggtcagcg acacattatg caaggccgga aagcgagcaa
Expressed Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MEVRVAGVRK EFARFPALHN VSLTIQSGEL IALLGPSGSG KTTLLRLIAG LETPTEGMIF FGDEDASQKS VQERNVGFVF QHYALFRHMT VADNIGFGLK VRPGGTRPST AEIRRRALEL LDLVQLSGLE KRYPAQLSGG QRQRVALARA MAIEPRVLLL DEPFGALDAQ VRKELRRWLR EIHDKTGHTT VFVTHDQDEA LELADRVVVM SQGRIEQVGT PDEVYDKPNS PFVYGFIGES STLPVRVENG EVWLADRNIG LGAENMPDGD AQLYFRPHDV ELLDGCGGCI AGTVIASRRS GGKRRVELEV GGARERIEIE IPAEHPAAEK SRIAFRPRYW TLFRAGDSEL PAPKAAATT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCACA TGAAAATCGA AGAAGGTAAA CTGGTAATCT GGATTAACGG CGATAAAGGC TATAACGGTC TCGCTGAAGT CGGTAAGAAA TTCGAGAAAG ATACCGGAAT TAAAGTCACC GTTGAGCATC CGGATAAACT GGAAGAGAAA TTCCCACAGG TTGCGGCAAC TGGCGATGGC CCTGACATTA TCTTCTGGGC ACACGACCGC TTTGGTGGCT ACGCTCAATC TGGCCTGTTG GCTGAAATCA CCCCGGACAA AGCGTTCCAG GACAAGCTGT ATCCGTTTAC CTGGGATGCC GTACGTTACA ACGGCAAGCT GATTGCTTAC CCGATCGCTG TTGAAGCGTT ATCGCTGATT TATAACAAAG ATCTGCTGCC GAACCCGCCA AAAACCTGGG AAGAGATCCC GGCGCTGGAT AAAGAACTGA AAGCGAAAGG TAAGAGCGCG CTGATGTTCA ACCTGCAAGA ACCGTACTTC ACCTGGCCGC TGATTGCTGC TGACGGGGGT TATGCGTTCA AGTATGAAAA CGGCAAGTAC GACATTAAAG ACGTGGGCGT GGATAACGCT GGCGCGAAAG CGGGTCTGAC CTTCCTGGTT GACCTGATTA AAAACAAACA CATGAATGCA GACACCGATT ACTCCATCGC AGAAGCTGCC TTTAATAAAG GCGAAACAGC GATGACCATC AACGGCCCGT GGGCATGGTC CAACATCGAC ACCAGCAAAG TGAATTATGG TGTAACGGTA CTGCCGACCT TCAAGGGTCA ACCATCCAAA CCGTTCGTTG GCGTGCTGAG CGCAGGTATT AACGCCGCCA GTCCGAACAA AGAGCTGGCA AAAGAGTTCC TCGAAAACTA TCTGCTGACT GATGAAGGTC TGGAAGCGGT TAATAAAGAC AAACCGCTGG GTGCCGTAGC GCTGAAGTCT TACGAGGAAG AGTTGGCGAA AGATCCACGT ATTGCCGCCA CTATGGAAAA CGCCCAGAAA GGTGAAATCA TGCCGAACAT CCCGCAGATG TCCGCTTTCT GGTATGCCGT GCGTACTGCG GTGATCAACG CCGCCAGCGG TCGTCAGACT GTCGATGAAG CCCTGAAAGA CGCGCAGACT AATTCGAGCT CGAACAACAA CAACAATAAC AATAACAACA ACCTCGGGAT CGAGGGAAGG ATTTCAGAAG GTACCCTGGA AGCTCAGACC CAGGGTCCTG GTTCGATGGA AGTTCGTGTT GCCGGCGTGC GTAAGGAGTT TGCTCGCTTT CCGGCCTTGC ATAATGTGTC GCTGACCATC CAGTCCGGCG AATTGATTGC CCTGCTTGGA CCATCGGGTT CGGGCAAGAC CACGCTTTTG CGCCTGATTG CGGGGCTGGA AACGCCAACC GAAGGCATGA TCTTCTTCGG GGATGAAGAT GCCTCGCAAA AGAGCGTGCA GGAGCGTAAT GTCGGTTTTG TCTTCCAGCA TTATGCGCTG TTCCGCCATA TGACGGTGGC GGATAATATC GGTTTCGGGT TGAAGGTTCG CCCCGGTGGG ACGCGCCCAT CCACGGCGGA AATTCGCCGC CGTGCGCTGG AGCTTCTTGA TCTCGTGCAG TTGAGCGGGC TGGAAAAACG CTATCCGGCG CAGCTTTCCG GCGGCCAGCG CCAGCGCGTG GCGCTGGCCC GCGCCATGGC GATCGAACCG CGTGTGCTTT TGCTGGATGA GCCTTTTGGT GCACTGGATG CGCAGGTGCG CAAGGAATTG CGCCGCTGGC TGCGCGAAAT CCACGACAAG ACCGGCCATA CCACGGTTTT CGTGACCCAT GACCAGGATG AGGCGCTGGA ACTTGCCGAC CGCGTGGTGG TGATGAGCCA GGGCCGCATC GAGCAGGTCG GCACGCCCGA CGAGGTCTAT GACAAGCCCA ACTCTCCTTT CGTGTACGGT TTCATCGGCG AGTCCAGCAC CCTGCCGGTG CGCGTGGAAA ACGGTGAGGT CTGGCTGGCG GATCGCAATA TCGGCCTTGG TGCGGAAAAT ATGCCGGATG GTGACGCGCA GCTCTATTTC CGCCCGCATG ATGTCGAGTT GCTCGACGGT TGCGGCGGCT GTATCGCGGG AACCGTGATT GCAAGCCGCC GTTCGGGTGG CAAGCGGCGC GTGGAACTGG AAGTGGGGGG CGCCCGCGAG CGCATCGAGA TAGAAATTCC GGCCGAGCAT CCGGCGGCAG AGAAAAGCCG CATTGCCTTT CGCCCGCGCT ACTGGACGCT CTTTCGCGCA GGCGATAGCG AACTTCCGGC GCCGAAGGCT GCGGCAACAA CTTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA