BrabA.01370.a

Phosphoglucosamine mutase (EC 5.4.2.10)

CENTER ID: BrabA.01370.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.01370.a.B1.GE37720 Full length( BrabA.01370.a ) 1 451
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel86222_1765
BV-BRC: fig|359391.4.peg.1765
RefSeq: WP_002964779.1
RefSeq: YP_415052.1
UniProt: Q2YQH8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRKFFGTDG IRGQANSFPM TPEIAMKVGM AVGYIFRRKG QASRAVVGKD TRRSGYMLEK ALVAGFTAAG MDVFLLGPIP TPAVAMLCRS LRADIGVMIS ASHNPFYDNG IKLFGPDGFK LSDQIELQIE AMIEGDMTPF LASHGDVGRA KRVDGDIYRY IEFAKRTLPR NISLNGLRVV VDCANGAGYK VAPAALWELG AEVITINNEP NGININEDCG STHPIGLMKK VHEVRADVGI ALDGDADRVL LVDENGTVID GDQLMAVIAE SWAASNRLEG GGIVATVMSN LGLERFLADR NLTLARTKVG DRYVVEHMRE HGFNVGGEQS GHIVLSDFAT TGDGLISALQ ILAVAQEQNK PISDVCRKFQ PVPQLLKNVR TTGGKPLENK RVKSAIDEAK ERLGGQGRLV IRPSGTEPLI RVMAEGDDRG LVEKVVNDII DVISSESSAA A
NT Sequence
ATGACACGGA AATTTTTTGG AACCGATGGC ATTCGCGGAC AGGCAAACAG CTTTCCGATG ACACCGGAAA TCGCCATGAA AGTGGGTATG GCCGTTGGCT ATATCTTCCG TCGCAAAGGT CAGGCCTCTC GCGCGGTGGT CGGCAAGGAT ACACGCCGCT CCGGCTATAT GCTGGAAAAA GCGCTGGTGG CCGGTTTTAC CGCTGCCGGC ATGGACGTGT TCCTGCTGGG CCCGATCCCG ACCCCTGCCG TCGCCATGCT CTGCCGCTCG CTGCGCGCTG ATATCGGGGT GATGATTTCC GCGTCGCATA ATCCCTTCTA CGATAACGGC ATCAAGCTTT TCGGCCCCGA TGGCTTCAAG CTTTCGGACC AGATCGAGCT TCAGATCGAG GCGATGATCG AAGGCGATAT GACGCCTTTT CTTGCGAGCC ATGGCGATGT TGGCCGCGCC AAGCGCGTGG ACGGCGATAT TTATCGCTAT ATCGAATTTG CCAAGCGCAC ATTGCCGCGC AACATCTCGC TCAATGGGCT GCGCGTTGTG GTCGATTGCG CGAATGGCGC GGGTTACAAG GTTGCGCCGG CTGCGCTGTG GGAACTGGGC GCGGAAGTCA TTACCATCAA CAACGAGCCG AATGGCATCA ATATCAACGA GGATTGCGGC TCGACGCATC CCATCGGCCT TATGAAGAAA GTGCATGAGG TGCGCGCCGA TGTGGGTATC GCGCTCGATG GCGATGCGGA TCGTGTGCTT CTGGTCGATG AAAACGGCAC CGTCATCGAT GGCGACCAGC TCATGGCTGT CATCGCCGAA AGCTGGGCTG CGAGCAATCG CCTGGAAGGC GGCGGCATTG TCGCCACTGT CATGTCCAAT CTCGGCCTGG AGCGTTTCTT GGCCGACCGT AATCTGACGC TTGCCCGTAC CAAGGTTGGC GACCGCTATG TGGTCGAGCA CATGCGCGAG CATGGTTTCA ATGTGGGTGG CGAGCAGTCG GGCCATATCG TTCTGTCGGA TTTTGCGACG ACTGGCGACG GTCTTATCTC CGCGCTCCAG ATACTTGCCG TGGCGCAGGA ACAGAACAAG CCGATCAGCG ATGTATGCCG CAAATTCCAG CCGGTGCCGC AATTGTTGAA GAATGTCCGC ACCACAGGCG GCAAACCACT TGAAAACAAA CGCGTGAAAT CAGCGATCGA TGAGGCCAAG GAACGCCTTG GCGGTCAGGG GCGACTGGTT ATCCGCCCGT CGGGCACGGA GCCGCTCATC CGCGTCATGG CGGAAGGTGA CGACCGTGGC CTGGTCGAAA AAGTGGTGAA CGACATCATT GATGTAATCT CTTCGGAAAG CAGTGCTGCG GCC
Details for BrabA.01370.a.B1.GE37720
HARVESTED ON: 2/5/2014
SEQUENCED ON: 2/6/2014
EXPECTED MW: 50kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 91
Validated AA Sequence
MTPEIAMKVG MAVGYIFRRK GQASRAVVGK DTRRSGYMLE KALVAGFTAA GMDVFLLGPI PTPAVAMLCR SLRADIGVMI SASHNPFYDN GIKLFGPDGF KLSDQIELQI EAMIEGDMTP FLASHGDVGR AKRVDGDIYR YIEFAKRTLP RNISLNGLRV VVDCANGAGY KVAPAALWEL GAEVITINNE PNGININEDC GSTHPIGLMK KVHEVRADVG IALDGDADRV LLVDENGTVI DGDQLMAVIA ESWAASNRLE GGGIVATVMS NLGLERFLAD RNLTLARTKV GDRYVVEHMR EHGFNVGGEQ SGHIVLSDFA TTGDGLISAL QILAVAQEQN KPISDVCRKF QPVPQLLKNV RTTGGKPLEN KRVKSAIDEA KERLGGQGRL VIRPSGTEPL IRVMAEGDDR GLVEKVVNDI ID
Validated NT Sequence
acatcaatga tgtcgttcac cactttttcg accaggccac ggtcgtcacc ttccgccatg acgcggatga gcggctccgt gcccgacggg cggataacca gtcgcccctg accgccaagg cgttccttgg cctcatcgat cgctgatttc acgcgtttgt tttcaagtgg tttgccgcct gtggtgcgga cattcttcaa caattgcggc accggctgga atttgcggca tacatcgctg atcggcttgt tctgttcctg cgccacggca agtatctgga gcgcggagat aagaccgtcg ccagtcgtcg caaaatccga cagaacgata tggcccgact gctcgccacc cacattgaaa ccatgctcgc gcatgtgctc gaccacatag cggtcgccaa ccttggtacg ggcaagcgtc agattacggt cggccaagaa acgctccagg ccgagattgg acatgacagt ggcgacaatg ccgccgcctt ccaggcgatt gctcgcagcc cagctttcgg cgatgacagc catgagctgg tcgccatcga tgacggtgcc gttttcatcg accagaagca cacgatccgc atcgccatcg agcgcgatac ccacatcggc gcgcacctca tgcactttct tcataaggcc gatgggatgc gtcgagccgc aatcctcgtt gatattgatg ccattcggct cgttgttgat ggtaatgact tccgcgccca gttcccacag cgcagccggc gcaaccttgt aacccgcgcc attcgcgcaa tcgaccacaa cgcgcagccc attgagcgag atgttgcgcg gcaatgtgcg cttggcaaat tcgatatagc gataaatatc gccgtccacg cgcttggcgc ggccaacatc gccatggctc gcaagaaaag gcgtcatatc gccttcgatc atcgcctcga tctgaagctc gatctggtcc gaaagcttga agccatcggg gccgaaaagc ttgatgccgt tatcgtagaa gggattatgc gacgcggaaa tcatcacccc gatatcagcg cgcagcgagc ggcagagcat ggcgacggca ggggtcggga tcgggcccag caggaacacg tccatgccgg cagcggtaaa accggccacc agcgcttttt ccagcatata gccggagcgg cgtgtatcct tgccgaccac cgcgcgagag gcctgacctt tgcgacggaa gatatagcca acggccatac ccactttcat ggcgatttcc ggtgtcatcg gaaagctgtt tgcctgtccg cgaatgccat cgattccaaa aaatttcc
Expected Protein Sequence
MAHHHHHHMT RKFFGTDGIR GQANSFPMTP EIAMKVGMAV GYIFRRKGQA SRAVVGKDTR RSGYMLEKAL VAGFTAAGMD VFLLGPIPTP AVAMLCRSLR ADIGVMISAS HNPFYDNGIK LFGPDGFKLS DQIELQIEAM IEGDMTPFLA SHGDVGRAKR VDGDIYRYIE FAKRTLPRNI SLNGLRVVVD CANGAGYKVA PAALWELGAE VITINNEPNG ININEDCGST HPIGLMKKVH EVRADVGIAL DGDADRVLLV DENGTVIDGD QLMAVIAESW AASNRLEGGG IVATVMSNLG LERFLADRNL TLARTKVGDR YVVEHMREHG FNVGGEQSGH IVLSDFATTG DGLISALQIL AVAQEQNKPI SDVCRKFQPV PQLLKNVRTT GGKPLENKRV KSAIDEAKER LGGQGRLVIR PSGTEPLIRV MAEGDDRGLV EKVVNDIIDV ISSESSAAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacac ggaaattttt tggaaccgat ggcattcgcg gacaggcaaa cagctttccg atgacaccgg aaatcgccat gaaagtgggt atggccgttg gctatatctt ccgtcgcaaa ggtcaggcct ctcgcgcggt ggtcggcaag gatacacgcc gctccggcta tatgctggaa aaagcgctgg tggccggttt taccgctgcc ggcatggacg tgttcctgct gggcccgatc ccgacccctg ccgtcgccat gctctgccgc tcgctgcgcg ctgatatcgg ggtgatgatt tccgcgtcgc ataatccctt ctacgataac ggcatcaagc ttttcggccc cgatggcttc aagctttcgg accagatcga gcttcagatc gaggcgatga tcgaaggcga tatgacgcct tttcttgcga gccatggcga tgttggccgc gccaagcgcg tggacggcga tatttatcgc tatatcgaat ttgccaagcg cacattgccg cgcaacatct cgctcaatgg gctgcgcgtt gtggtcgatt gcgcgaatgg cgcgggttac aaggttgcgc cggctgcgct gtgggaactg ggcgcggaag tcattaccat caacaacgag ccgaatggca tcaatatcaa cgaggattgc ggctcgacgc atcccatcgg ccttatgaag aaagtgcatg aggtgcgcgc cgatgtgggt atcgcgctcg atggcgatgc ggatcgtgtg cttctggtcg atgaaaacgg caccgtcatc gatggcgacc agctcatggc tgtcatcgcc gaaagctggg ctgcgagcaa tcgcctggaa ggcggcggca ttgtcgccac tgtcatgtcc aatctcggcc tggagcgttt cttggccgac cgtaatctga cgcttgcccg taccaaggtt ggcgaccgct atgtggtcga gcacatgcgc gagcatggtt tcaatgtggg tggcgagcag tcgggccata tcgttctgtc ggattttgcg acgactggcg acggtcttat ctccgcgctc cagatacttg ccgtggcgca ggaacagaac aagccgatca gcgatgtatg ccgcaaattc cagccggtgc cgcaattgtt gaagaatgtc cgcaccacag gcggcaaacc acttgaaaac aaacgcgtga aatcagcgat cgatgaggcc aaggaacgcc ttggcggtca ggggcgactg gttatccgcc cgtcgggcac ggagccgctc atccgcgtca tggcggaagg tgacgaccgt ggcctggtcg aaaaagtggt gaacgacatc attgatgtaa tctcttcgga aagcagtgct gcggcctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t