BrabA.01141.a
Fructokinase (EC 2.7.1.4)
| CENTER ID: | BrabA.01141.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.01141.a.B1.GE37718 | Full length( BrabA.01141.a ) | 1 | 308 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.01141.a.B1.PS02024 | Full length( BrabA.01141.a ) | 1 | 308 |
Structures
4U7X
DEPOSITED: 7/31/2014
DETERMINATION: XRay
CLONE: BrabA.01141.a.B1.GE37718
PROTEIN: BrabA.01141.a.B1.PS02024
4WJM
DEPOSITED: 10/1/2014
DETERMINATION: XRay
CLONE: BrabA.01141.a.B1.GE37718
PROTEIN: BrabA.01141.a.B1.PS02024
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|359391.4.peg.119 |
| RefSeq: | YP_413608.1 |
| UniProt: | Q2YNY7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MILCCGEALI DMLPRETTGG ETAFQPFAGG SVFNTAIALG RLGVPTGFFS GISSDFFGDV LRDTLARSNV DYSFAAISNR PTTLAFVRLV DGQARYAFYD ENTAGRMLSR NDMPYVDETI SAMLFGCISL ISEPCGSVYE TLLAREAPNR VMFLDPNIRA NLITVRKTHL TRMKRMIALA DIVKLSDEDL DWFGEKGSHD EIAAEWLKLG PKLVVITKGA HGAVAYTNHA TVPVPGVKVD VVDTVGAGDT VNAGILASLH SQGLLTKDAL ANLSEDQIHS AVALGVRAAA VTVSRAGANP PWAHEMRD
NT Sequence
ATGATCCTTT GTTGCGGTGA AGCCCTGATC GACATGCTGC CACGCGAAAC CACTGGCGGC GAAACCGCAT TCCAGCCTTT CGCCGGCGGT TCGGTTTTCA ACACGGCCAT TGCGCTGGGG CGGCTTGGCG TTCCGACCGG CTTTTTCTCC GGCATTTCAT CGGATTTTTT CGGCGATGTG CTGCGCGACA CGCTGGCGCG CTCCAATGTC GATTATTCCT TCGCCGCCAT TTCCAACAGG CCGACAACGC TTGCCTTCGT GCGCCTCGTG GACGGTCAGG CACGCTATGC TTTCTATGAC GAAAACACGG CCGGGCGAAT GCTGTCGAGA AACGACATGC CCTATGTGGA TGAAACCATA AGCGCCATGC TGTTCGGCTG CATCAGCCTT ATTTCCGAAC CCTGCGGCAG CGTCTATGAG ACGCTGCTGG CGCGGGAAGC ACCAAACCGG GTGATGTTCC TCGACCCCAA TATTCGCGCA AACCTCATCA CCGTCCGTAA AACGCACCTG ACGCGCATGA AGCGCATGAT CGCGCTTGCC GATATCGTAA AACTCTCCGA CGAAGACCTG GACTGGTTCG GGGAAAAAGG CAGCCATGAC GAGATTGCCG CCGAATGGCT GAAGCTTGGG CCGAAACTTG TCGTCATCAC CAAGGGCGCA CATGGCGCTG TTGCCTATAC AAACCATGCA ACCGTGCCTG TGCCGGGCGT GAAGGTGGAT GTGGTTGATA CGGTGGGCGC GGGCGACACG GTCAATGCGG GAATTCTGGC GAGCCTCCAC AGTCAGGGCC TTCTGACGAA GGACGCGCTT GCGAACCTGA GCGAAGACCA GATACATTCC GCCGTGGCGC TTGGGGTACG GGCTGCTGCC GTGACCGTCT CGCGCGCAGG TGCGAACCCG CCATGGGCGC ATGAGATGCG GGAT
Details for BrabA.01141.a.B1.GE37718
| HARVESTED ON: | 2/5/2014 |
| SEQUENCED ON: | 2/6/2014 |
| EXPECTED MW: | 34kDa |
| OBSERVED MW: | 34kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMI LCCGEALIDM LPRETTGGET AFQPFAGGSV FNTAIALGRL GVPTGFFSGI SSDFFGDVLR DTLARSNVDY SFAAISNRPT TLAFVRLVDG QARYAFYDEN TAGRMLSRND MPYVDETISA MLFGCISLIS EPCGSVYETL LAREAPNRVM FLDPNIRANL ITVRKTHLTR MKRMIALADI VKLSDEDLDW FGEKGSHDEI AAEWLKLGPK LVVITKGAHG AVAYTNHATV PVPGVKVDVV DTVGAGDTVN AGILASLHSQ GLLTKDALAN LSEDQIHSAV ALGVRAAAVT VSRAGANPPW AHEMRD
Validated NT Sequence
tactcactta atcccgcatc tcatgcgccc atggcgggtt cgcacctgcg cgcgagacgg tcacggcagc agcccgtacc ccaagcgcca cggcggaatg tatctggtct tcgctcaggt tcgcaagcgc gtccttcgtc agaaggccct gactgtggag gctcgccaga attcccgcat tgaccgtgtc gcccgcgccc accgtatcaa ccacatccac cttcacgccc ggcacaggca cggttgcatg gtttgtatag gcaacagcgc catgtgcgcc cttggtgatg acgacaagtt tcggcccaag cttcagccat tcggcggcaa tctcgtcatg gctgcctttt tccccgaacc agtccaggtc ttcgtcggag agttttacga tatcggcaag cgcgatcatg cgcttcatgc gcgtcaggtg cgttttacgg acggtgatga ggtttgcgcg aatattgggg tcgaggaaca tcacccggtt tggtgcttcc cgcgccagca gcgtctcata gacgctgccg cagggttcgg aaataaggct gatgcagccg aacagcatgg cgcttatggt ttcatccaca tagggcatgt cgtttctcga cagcattcgc ccggccgtgt tttcgtcata gaaagcatag cgtgcctgac cgtccacgag gcgcacgaag gcaagcgttg tcggcctgtt ggaaatggcg gcgaaggaat aatcgacatt ggagcgcgcc agcgtgtcgc gcagcacatc gccgaaaaaa tccgatgaaa tgccggagaa aaagccggtc ggaacgccaa gccgccccag cgcaatggcc gtgttgaaaa ccgaaccgcc ggcgaaaggc tggaatgcgg tttcgccgcc agtggtttcg cgtggcagca tgtcgatcag ggcttcaccg caacaaagga tcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMI LCCGEALIDM LPRETTGGET AFQPFAGGSV FNTAIALGRL GVPTGFFSGI SSDFFGDVLR DTLARSNVDY SFAAISNRPT TLAFVRLVDG QARYAFYDEN TAGRMLSRND MPYVDETISA MLFGCISLIS EPCGSVYETL LAREAPNRVM FLDPNIRANL ITVRKTHLTR MKRMIALADI VKLSDEDLDW FGEKGSHDEI AAEWLKLGPK LVVITKGAHG AVAYTNHATV PVPGVKVDVV DTVGAGDTVN AGILASLHSQ GLLTKDALAN LSEDQIHSAV ALGVRAAAVT VSRAGANPPW AHEMRD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatcc tttgttgcgg tgaagccctg atcgacatgc tgccacgcga aaccactggc ggcgaaaccg cattccagcc tttcgccggc ggttcggttt tcaacacggc cattgcgctg gggcggcttg gcgttccgac cggctttttc tccggcattt catcggattt tttcggcgat gtgctgcgcg acacgctggc gcgctccaat gtcgattatt ccttcgccgc catttccaac aggccgacaa cgcttgcctt cgtgcgcctc gtggacggtc aggcacgcta tgctttctat gacgaaaaca cggccgggcg aatgctgtcg agaaacgaca tgccctatgt ggatgaaacc ataagcgcca tgctgttcgg ctgcatcagc cttatttccg aaccctgcgg cagcgtctat gagacgctgc tggcgcggga agcaccaaac cgggtgatgt tcctcgaccc caatattcgc gcaaacctca tcaccgtccg taaaacgcac ctgacgcgca tgaagcgcat gatcgcgctt gccgatatcg taaaactctc cgacgaagac ctggactggt tcggggaaaa aggcagccat gacgagattg ccgccgaatg gctgaagctt gggccgaaac ttgtcgtcat caccaagggc gcacatggcg ctgttgccta tacaaaccat gcaaccgtgc ctgtgccggg cgtgaaggtg gatgtggttg atacggtggg cgcgggcgac acggtcaatg cgggaattct ggcgagcctc cacagtcagg gccttctgac gaaggacgcg cttgcgaacc tgagcgaaga ccagatacat tccgccgtgg cgcttggggt acgggctgct gccgtgaccg tctcgcgcgc aggtgcgaac ccgccatggg cgcatgagat gcgggattga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BrabA.01141.a.B1.PS02024
| PURIFICATION DATe: | 3/25/2014 |
| CONCENTRATION: | 38mg/ml |
| OBSERVED MW: | 32kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMI LCCGEALIDM LPRETTGGET AFQPFAGGSV FNTAIALGRL GVPTGFFSGI SSDFFGDVLR DTLARSNVDY SFAAISNRPT TLAFVRLVDG QARYAFYDEN TAGRMLSRND MPYVDETISA MLFGCISLIS EPCGSVYETL LAREAPNRVM FLDPNIRANL ITVRKTHLTR MKRMIALADI VKLSDEDLDW FGEKGSHDEI AAEWLKLGPK LVVITKGAHG AVAYTNHATV PVPGVKVDVV DTVGAGDTVN AGILASLHSQ GLLTKDALAN LSEDQIHSAV ALGVRAAAVT VSRAGANPPW AHEMRD
Validated NT Sequence
tactcactta atcccgcatc tcatgcgccc atggcgggtt cgcacctgcg cgcgagacgg tcacggcagc agcccgtacc ccaagcgcca cggcggaatg tatctggtct tcgctcaggt tcgcaagcgc gtccttcgtc agaaggccct gactgtggag gctcgccaga attcccgcat tgaccgtgtc gcccgcgccc accgtatcaa ccacatccac cttcacgccc ggcacaggca cggttgcatg gtttgtatag gcaacagcgc catgtgcgcc cttggtgatg acgacaagtt tcggcccaag cttcagccat tcggcggcaa tctcgtcatg gctgcctttt tccccgaacc agtccaggtc ttcgtcggag agttttacga tatcggcaag cgcgatcatg cgcttcatgc gcgtcaggtg cgttttacgg acggtgatga ggtttgcgcg aatattgggg tcgaggaaca tcacccggtt tggtgcttcc cgcgccagca gcgtctcata gacgctgccg cagggttcgg aaataaggct gatgcagccg aacagcatgg cgcttatggt ttcatccaca tagggcatgt cgtttctcga cagcattcgc ccggccgtgt tttcgtcata gaaagcatag cgtgcctgac cgtccacgag gcgcacgaag gcaagcgttg tcggcctgtt ggaaatggcg gcgaaggaat aatcgacatt ggagcgcgcc agcgtgtcgc gcagcacatc gccgaaaaaa tccgatgaaa tgccggagaa aaagccggtc ggaacgccaa gccgccccag cgcaatggcc gtgttgaaaa ccgaaccgcc ggcgaaaggc tggaatgcgg tttcgccgcc agtggtttcg cgtggcagca tgtcgatcag ggcttcaccg caacaaagga tcatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHMI LCCGEALIDM LPRETTGGET AFQPFAGGSV FNTAIALGRL GVPTGFFSGI SSDFFGDVLR DTLARSNVDY SFAAISNRPT TLAFVRLVDG QARYAFYDEN TAGRMLSRND MPYVDETISA MLFGCISLIS EPCGSVYETL LAREAPNRVM FLDPNIRANL ITVRKTHLTR MKRMIALADI VKLSDEDLDW FGEKGSHDEI AAEWLKLGPK LVVITKGAHG AVAYTNHATV PVPGVKVDVV DTVGAGDTVN AGILASLHSQ GLLTKDALAN LSEDQIHSAV ALGVRAAAVT VSRAGANPPW AHEMRD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATCC TTTGTTGCGG TGAAGCCCTG ATCGACATGC TGCCACGCGA AACCACTGGC GGCGAAACCG CATTCCAGCC TTTCGCCGGC GGTTCGGTTT TCAACACGGC CATTGCGCTG GGGCGGCTTG GCGTTCCGAC CGGCTTTTTC TCCGGCATTT CATCGGATTT TTTCGGCGAT GTGCTGCGCG ACACGCTGGC GCGCTCCAAT GTCGATTATT CCTTCGCCGC CATTTCCAAC AGGCCGACAA CGCTTGCCTT CGTGCGCCTC GTGGACGGTC AGGCACGCTA TGCTTTCTAT GACGAAAACA CGGCCGGGCG AATGCTGTCG AGAAACGACA TGCCCTATGT GGATGAAACC ATAAGCGCCA TGCTGTTCGG CTGCATCAGC CTTATTTCCG AACCCTGCGG CAGCGTCTAT GAGACGCTGC TGGCGCGGGA AGCACCAAAC CGGGTGATGT TCCTCGACCC CAATATTCGC GCAAACCTCA TCACCGTCCG TAAAACGCAC CTGACGCGCA TGAAGCGCAT GATCGCGCTT GCCGATATCG TAAAACTCTC CGACGAAGAC CTGGACTGGT TCGGGGAAAA AGGCAGCCAT GACGAGATTG CCGCCGAATG GCTGAAGCTT GGGCCGAAAC TTGTCGTCAT CACCAAGGGC GCACATGGCG CTGTTGCCTA TACAAACCAT GCAACCGTGC CTGTGCCGGG CGTGAAGGTG GATGTGGTTG ATACGGTGGG CGCGGGCGAC ACGGTCAATG CGGGAATTCT GGCGAGCCTC CACAGTCAGG GCCTTCTGAC GAAGGACGCG CTTGCGAACC TGAGCGAAGA CCAGATACAT TCCGCCGTGG CGCTTGGGGT ACGGGCTGCT GCCGTGACCG TCTCGCGCGC AGGTGCGAAC CCGCCATGGG CGCATGAGAT GCGGGATTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT