SSGCID: Target Page

BrabA.01123.b

Dihydropyrimidinase (EC 3.5.2.2)

CENTER ID:	BrabA.01123.b
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.01123.b.B1.GE37717	Full length( BrabA.01123.b )		1	302

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel86222_0328

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAKTGASKVI KGGTVITADR TFRADILIED GKIAAIGDSL EGDEVIDASG CYVMRGGIDP HTHLQMPFMG TYSSDDFDTG TAAALAGGTT MVVDFVLPDS EGNLLDALQE WFQKAGKART DYSFHMAITG WNERTFNEMV EVVKRGINTF KHFMAYKGAL MVNDDEMFAS FQRCAELGAM PLVHAENGDI VAQLQAKLMA EGNDGPEAHA YSRPPEVEGE ATNRAIMIAD QAGVPLYVVH VSCEQSHEAI RRARQKGIRV FGEPLIQHLT LDESEYHNRD WDYAARRVMS PPFRDKLNQD SL

NT Sequence

ATGGCCAAGA CCGGGGCAAG CAAAGTCATC AAGGGCGGCA CCGTCATCAC GGCTGACCGC ACGTTCAGGG CAGATATTCT TATCGAAGAC GGCAAGATCG CCGCCATTGG CGATAGCCTT GAAGGCGATG AGGTCATCGA CGCATCCGGC TGCTATGTCA TGCGGGGCGG CATCGACCCG CACACCCACC TGCAAATGCC CTTCATGGGC ACATATTCCT CCGACGATTT CGACACGGGC ACGGCGGCAG CACTTGCCGG CGGCACGACG ATGGTGGTCG ATTTCGTCCT GCCGGATTCC GAGGGCAATC TTCTGGATGC GCTTCAGGAA TGGTTTCAGA AGGCGGGCAA GGCGCGCACC GATTATTCGT TCCACATGGC GATCACCGGC TGGAACGAGC GCACTTTCAA CGAAATGGTC GAAGTGGTGA AGCGCGGCAT CAACACGTTC AAGCATTTCA TGGCCTATAA GGGCGCGCTG ATGGTGAATG ACGATGAGAT GTTCGCCTCG TTCCAGCGTT GTGCGGAACT GGGTGCCATG CCGCTCGTCC ATGCGGAAAA CGGCGATATC GTCGCGCAAT TGCAGGCGAA ACTCATGGCC GAAGGCAATG ACGGGCCGGA AGCCCATGCC TATTCCCGCC CGCCGGAAGT GGAAGGAGAA GCCACCAATC GCGCCATCAT GATCGCCGAT CAGGCGGGCG TACCGCTTTA TGTGGTGCAT GTCTCCTGCG AGCAAAGCCA TGAAGCAATC CGCCGCGCGC GCCAGAAGGG TATACGTGTT TTCGGCGAGC CGCTGATCCA GCATCTGACG CTCGATGAAA GCGAATATCA CAATCGCGAC TGGGACTATG CCGCGCGGCG CGTGATGTCG CCGCCGTTCC GCGACAAGCT CAATCAGGAC AGCCTC

Details for BrabA.01123.b.B1.GE37717

HARVESTED ON:	2/5/2014
SEQUENCED ON:	2/7/2014
EXPECTED MW:	34kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA KTGASKVIKG GTVITADRTF RADILIEDGK IAAIGDSLEG DEVIDASGCY VMRGGIDPHT HLQMPFMGTY SSDDFDTGTA AALAGGTTMV VDFVLPDSEG NLLDALQEWF QKAGKARTDY SFHMAITGWN ERTFNEMVEV VKRGINTFKH FMAYKGALMV NDDEMFASFQ RCAELGAMPL VHAENGDIVA QLQAKLMAEG NDGPEAHAYS RPPEVEGEAT NRAIMIADQA GVPLYVVHVS CEQSHEAIRR ARQKGIRVFG EPLIQHLTLD ESEYHNRDWD YAARRVMSPP FRDKLNQDSL

Validated NT Sequence

cnnnnnnnnn tnnntanata ttttgtttaa ctttaagaag gagatatacc atggctcacc accaccacca ccatatggcc aagaccgggg caagcaaagt catcaagggc ggcaccgtca tcacggctga ccgcacgttc agggcagata ttcttatcga agacggcaag atcgccgcca ttggcgatag ccttgaaggc gatgaggtca tcgacgcatc cggctgctat gtcatgcggg gcggcatcga cccgcacacc cacctgcaaa tgcccttcat gggcacatat tcctccgacg atttcgacac gggcacggcg gcagcacttg ccggcggcac gacgatggtg gtcgatttcg tcctgccgga ttccgagggc aatcttctgg atgcgcttca ggaatggttt cagaaggcgg gcaaggcgcg caccgattat tcgttccaca tggcgatcac cggctggaac gagcgcactt tcaacgaaat ggtcgaagtg gtgaagcgcg gcatcaacac gttcaagcat ttcatggcct ataagggcgc gctgatggtg aatgacgatg agatgttcgc ctcgttccag cgttgtgcgg aactgggtgc catgccgctc gtccatgcgg aaaacggcga tatcgtcgcg caattgcagg cgaaactcat ggccgaaggc aatgacgggc cggaagccca cgcctattcc cgcccgccgg aagtggaagg agaagccacc aatcgcgcca tcatgatcgc cgatcaggcg ggcgtaccgc tttatgtggt gcatgtctcc tgcgagcaaa gccatgaagc aatccgccgc gcgcgccaga agggtatacg tgttttcggc gagccgctga tccagcatct gacgctcgat gaaagcgaat atcacaatcg cgactgggac tatgccgcgc ggcgcgtgat gtcgccgccg ttccgcgaca agctcaatca ggacagcctc taagtgagta agatagnncc gtnnnncccc cccnnnnnnn annnnnnnng g

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcca agaccggggc aagcaaagtc atcaagggcg gcaccgtcat cacggctgac cgcacgttca gggcagatat tcttatcgaa gacggcaaga tcgccgccat tggcgatagc cttgaaggcg atgaggtcat cgacgcatcc ggctgctatg tcatgcgggg cggcatcgac ccgcacaccc acctgcaaat gcccttcatg ggcacatatt cctccgacga tttcgacacg ggcacggcgg cagcacttgc cggcggcacg acgatggtgg tcgatttcgt cctgccggat tccgagggca atcttctgga tgcgcttcag gaatggtttc agaaggcggg caaggcgcgc accgattatt cgttccacat ggcgatcacc ggctggaacg agcgcacttt caacgaaatg gtcgaagtgg tgaagcgcgg catcaacacg ttcaagcatt tcatggccta taagggcgcg ctgatggtga atgacgatga gatgttcgcc tcgttccagc gttgtgcgga actgggtgcc atgccgctcg tccatgcgga aaacggcgat atcgtcgcgc aattgcaggc gaaactcatg gccgaaggca atgacgggcc ggaagcccat gcctattccc gcccgccgga agtggaagga gaagccacca atcgcgccat catgatcgcc gatcaggcgg gcgtaccgct ttatgtggtg catgtctcct gcgagcaaag ccatgaagca atccgccgcg cgcgccagaa gggtatacgt gttttcggcg agccgctgat ccagcatctg acgctcgatg aaagcgaata tcacaatcgc gactgggact atgccgcgcg gcgcgtgatg tcgccgccgt tccgcgacaa gctcaatcag gacagcctct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat