SSGCID: Target Page

BrabA.00629.b

Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases

CENTER ID:	BrabA.00629.b
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.00629.b.B1.GE37716	Full length( BrabA.00629.b )		1	300

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel86222_3082

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTGPLLLGVP TILNEGGFTA ENTYDIIERL GVTSLAGSPT AFRLLMAAGP ESAARVKGRL RVASSAGEPL NPEVIRWFDA CLGAPIHDHY GQTELGMVVN NHHGLEHPVR QGSAGYAMPG YRIAVLDEAG KEVGPNEPGV LAIDIDNSPL LWFTGYYKKD TPSISGGYYR TGDTVEFEPD GSISFIGRAD DVITSSGYRI GPFDVESALL EHPAVNEAAV VGVPDPQRTE IVKAFVILAP GFEGTPELAE ELALHVKKQL SAHAYPRQID FVAELPKTPS GKIQRFLLRK AEVEKQQQQN

NT Sequence

GTGACGGGTC CCCTGCTTCT GGGTGTGCCG ACGATCCTGA ACGAAGGCGG TTTCACCGCC GAAAACACCT ATGACATCAT CGAACGCCTC GGCGTGACGA GCCTTGCGGG CTCTCCGACT GCCTTCCGCC TGCTTATGGC CGCCGGGCCG GAATCTGCTG CCCGCGTCAA GGGCCGCTTG CGCGTGGCAT CCAGCGCGGG CGAGCCGCTG AACCCGGAAG TCATTCGCTG GTTCGATGCC TGTCTTGGCG CGCCGATCCA CGATCATTAC GGCCAGACGG AACTGGGCAT GGTGGTGAAC AACCATCATG GATTGGAACA TCCGGTGCGG CAGGGTTCGG CGGGCTATGC CATGCCGGGC TACAGGATTG CGGTTCTGGA TGAAGCGGGC AAAGAAGTCG GTCCCAATGA ACCGGGCGTT CTGGCCATTG ATATCGACAA TTCGCCGCTT CTCTGGTTCA CCGGCTATTA TAAAAAGGAT ACGCCCTCCA TCTCCGGCGG ATATTATCGC ACCGGCGATA CGGTGGAATT CGAGCCGGAT GGGTCTATCA GCTTCATCGG CCGCGCCGAT GACGTCATCA CCTCCTCCGG CTATCGCATC GGGCCTTTCG ATGTGGAAAG CGCGCTTCTG GAACATCCTG CGGTCAACGA GGCTGCGGTG GTCGGCGTTC CCGATCCGCA ACGCACCGAA ATCGTGAAGG CCTTTGTCAT CCTGGCCCCC GGCTTCGAGG GTACGCCGGA ACTGGCGGAA GAACTGGCGC TTCACGTCAA GAAACAGCTT TCCGCCCATG CCTATCCGCG GCAGATCGAC TTTGTGGCGG AGCTTCCCAA GACGCCGAGC GGAAAAATAC AGCGCTTCCT CCTGCGCAAG GCGGAAGTCG AAAAGCAACA GCAACAGAAT

Details for BrabA.00629.b.B1.GE37716

HARVESTED ON:	2/5/2014
SEQUENCED ON:	2/6/2014
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV TGPXXLGVPT ILNEGGFTAE NTYDIIERLG VTSLAGSPTA FRLLMAAGPE SAARVKGRLR VASSAGEPLN PEVIRWFDAC LGAPIHDHYG QTELGMVVNN HHGLEHPVRQ GSAGYAMPGY RIAVLDEAGK EVGPNEPGVL AIDIDNSPLL WFTGYYKKDT PSISGGYYRT GDTVEFEPDG SISFIGRADD VITSSGYRIG PFDVESALLE HPAVNEAAVV GVPDPQRTEI VKAFVILAPG FEGTPELAEE LALHVKKQLS AHAYPRQIDF VAELPKTPSG KIQRFLLRKA EVEKQQQQN

Validated NT Sequence

tactcactta attctgttgc tgttgctttt cgacttccgc cttgcgcagg aggaagcgct gtatttttcc gctcggcgtc ttgggaagct ccgccacaaa gtcgatctgc cgcggatagg catgggcgga aagctgtttc ttgacgtgaa gcgccagttc ttccgccagt tccggcgtac cctcgaagcc gggggccagg atgacaaagg ccttcacgat ttcggtgcgt tgcggatcgg gaacgccgac caccgcagcc tcgttgaccg caggatgttc cagaagcgcg ctttccacat cgaaaggccc gatgcgatag ccggaggagg tgatgacgtc atcggcgcgg ccgatgaagc tgatagaccc atccggctcg aattccaccg tatcgccggt gcgataatat ccgccggaga tggagggcgt atccttttta taatagccgg tgaaccagag aagcggcgaa ttgtcgatat caatggccag aacgcccggt tcattgggac cgacttcttt gcccgcttca tccagaaccg caatcctgta gcccggcatg gcatagcccg ccgaaccctg ccgcaccgga tgttccaatc catgatggtt gttcaccacc atgcccagtt ccgtctggcc gtaatgatcg tggatcggcg cgccaagaca ggcatcgaac cagcgaatga cttccgggtt cagcggctcg cccgcgctgg atgccacgcg caagcggccc ttgacgcggg cagcagattc cggcccggcg gccataagca ggcggaaggc agtcggagag cccgcaaggc tcgtcacgcc gaggcgttcg atgatgtcat aggtgttttc ggcggtgaaa ccgccttcgt tcaggatcgt cggcacaccc agaancnngg gacccgtcac catatggtgg tggtggtggt gagccat

Expected Protein Sequence

MAHHHHHHMT GPLLLGVPTI LNEGGFTAEN TYDIIERLGV TSLAGSPTAF RLLMAAGPES AARVKGRLRV ASSAGEPLNP EVIRWFDACL GAPIHDHYGQ TELGMVVNNH HGLEHPVRQG SAGYAMPGYR IAVLDEAGKE VGPNEPGVLA IDIDNSPLLW FTGYYKKDTP SISGGYYRTG DTVEFEPDGS ISFIGRADDV ITSSGYRIGP FDVESALLEH PAVNEAAVVG VPDPQRTEIV KAFVILAPGF EGTPELAEEL ALHVKKQLSA HAYPRQIDFV AELPKTPSGK IQRFLLRKAE VEKQQQQN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgacgg gtcccctgct tctgggtgtg ccgacgatcc tgaacgaagg cggtttcacc gccgaaaaca cctatgacat catcgaacgc ctcggcgtga cgagccttgc gggctctccg actgccttcc gcctgcttat ggccgccggg ccggaatctg ctgcccgcgt caagggccgc ttgcgcgtgg catccagcgc gggcgagccg ctgaacccgg aagtcattcg ctggttcgat gcctgtcttg gcgcgccgat ccacgatcat tacggccaga cggaactggg catggtggtg aacaaccatc atggattgga acatccggtg cggcagggtt cggcgggcta tgccatgccg ggctacagga ttgcggttct ggatgaagcg ggcaaagaag tcggtcccaa tgaaccgggc gttctggcca ttgatatcga caattcgccg cttctctggt tcaccggcta ttataaaaag gatacgccct ccatctccgg cggatattat cgcaccggcg atacggtgga attcgagccg gatgggtcta tcagcttcat cggccgcgcc gatgacgtca tcacctcctc cggctatcgc atcgggcctt tcgatgtgga aagcgcgctt ctggaacatc ctgcggtcaa cgaggctgcg gtggtcggcg ttcccgatcc gcaacgcacc gaaatcgtga aggcctttgt catcctggcc cccggcttcg agggtacgcc ggaactggcg gaagaactgg cgcttcacgt caagaaacag ctttccgccc atgcctatcc gcggcagatc gactttgtgg cggagcttcc caagacgccg agcggaaaaa tacagcgctt cctcctgcgc aaggcggaag tcgaaaagca acagcaacag aattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat