SSGCID: Target Page

BrabA.00339.a

Alanine racemase

CENTER ID:	BrabA.00339.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.00339.a.A1.GE31545	Full length( BrabA.00339.a )		1	396

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.00339.a.A1.PW31443	Full length( BrabA.00339.a )		1	396

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.2606
RefSeq:	YP_418523.1
UniProt:	Q2YIU9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLPFSQDER DLAAGGILTI DLAALRHNYS AIATRIAPTR TAAVVKADAY GLGASRVAPA FYEAGCRDFF VAHLGEAVAL KPFLKPDATL YVLNGLQPGT EAACAREGIL PVLNSLEQVE NWAALATRLG KKLPALLQFD TGMSRLGLSA KEFDRLLENV TLLSRIDIKF AISHLANGDE PGNAVNARQL AKMTALLARL PKLPAALANS GGTFLGKTYY FDLARPGIAL YGIDPERQHD FSDKVAHENK KPKHSILPVL TLSARVIQVR DVDKGATVGY GGTYVANGPM RIATIAVGYA DGLFRSLSNK GAAFFGDTRL PIIGRVSMDS ITLDVTSLPE GTLKLGSLVE LIGPHQRLED VARDCDTIPY EILTALGNRY ARVYVYVNGG GTSTTA

NT Sequence

ATGTCTTTAC CTTTTTCTCA GGATGAGCGC GATCTCGCGG CTGGCGGCAT ATTGACCATT GATCTCGCAG CCCTGCGCCA CAATTATTCA GCCATCGCAA CCCGCATTGC CCCAACCCGT ACGGCCGCTG TGGTAAAGGC AGACGCCTAT GGCCTGGGCG CAAGCCGGGT CGCACCGGCT TTCTACGAGG CCGGTTGCCG CGATTTCTTC GTTGCACATC TTGGCGAAGC AGTGGCGCTG AAGCCCTTTT TGAAACCCGA CGCAACACTT TATGTCCTGA ACGGCCTTCA GCCCGGAACG GAAGCAGCCT GTGCCCGCGA AGGCATCCTG CCTGTTCTCA ATTCGCTGGA ACAGGTTGAA AACTGGGCAG CACTCGCCAC CCGGCTGGGC AAAAAACTGC CCGCCCTGCT CCAGTTCGAT ACCGGCATGT CTCGTCTTGG CCTGTCAGCC AAGGAATTTG ACCGCCTTCT TGAGAACGTC ACGCTCCTGA GCAGGATCGA CATCAAATTC GCCATCAGCC ATCTGGCAAA CGGCGACGAG CCGGGAAATG CGGTAAATGC CCGTCAGCTT GCCAAGATGA CGGCCTTGCT CGCTCGCCTG CCGAAACTGC CGGCTGCCTT GGCCAATTCC GGCGGCACCT TCCTCGGCAA GACCTATTAT TTCGATCTCG CCCGCCCCGG CATCGCGCTA TATGGCATCG ATCCGGAGAG GCAGCATGAC TTTTCGGATA AGGTGGCGCA CGAGAATAAG AAACCCAAGC ATTCGATCCT TCCCGTCCTC ACCCTTTCGG CGCGTGTCAT TCAGGTGCGC GACGTCGACA AGGGAGCGAC AGTTGGCTAT GGCGGCACCT ATGTCGCCAA CGGCCCGATG CGGATTGCGA CGATTGCGGT CGGCTATGCT GACGGCTTGT TCCGTTCCTT GAGTAACAAG GGCGCAGCCT TTTTCGGCGA TACGCGCCTG CCCATCATCG GGCGCGTATC GATGGATTCG ATCACGCTCG ACGTCACCTC ATTGCCGGAG GGAACGCTGA AACTCGGCAG CCTTGTGGAA TTGATCGGCC CGCATCAGCG CCTCGAAGAC GTGGCGCGGG ACTGCGACAC TATCCCCTAT GAAATTCTGA CTGCGCTTGG CAACCGTTAT GCGCGCGTTT ACGTTTACGT GAATGGCGGT GGCACCAGCA CAACAGCA

Details for BrabA.00339.a.A1.GE31545

HARVESTED ON:	2/1/2011
SEQUENCED ON:	2/8/2011
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	8
PERCENT COVERAGE:	70

Validated AA Sequence

MXXXXXXXXP XXXXGTLEXX TPGPGSXSLP FSXXXXDLXX XXXXXXXXXX XXXXXXXXXX XXPPPXXXXX XXXXXXXXXX HXXXXGXXXS XXXXAXXXXX XXXXXXXXXX VXXXPXPXXX XXXCPRXXXX XXXXXXRXXX XXXXXXXXXK XXXXXXSXFX XXXXRXXXXX XXXXXXXLXX XXXXXXXXXX XXXXXXXXXX XXXXXVXXXX XXXXXXCXXX XXXXXXXXXX XXXXGXXXXX XXXXXXXXXX XXXXIXXXXX XXXXXXXXXX XKXXXXXXXX XXXXGXXXXX XXXXXX

Validated NT Sequence

annnncgnnn nngccatgnn nnnnnnnnnc cntgnnnnnn naccnnnann nnnanngggt accctggaan ctcanacccc gggtcctggn tcnangtcnt taccnttttc ncnngangnn nnngatctcc ngncngnntn tngnncnntg ntnnncnann cnngnncnnc nntantcncn nnnnnnannc nnantnnncn nccnccnccn nnnnnngngn nnnannannn cnnnnnnnnn nnnnnnngcg ngnnncacnn nanntnnncn ggcngnnnnt nntcnnngan nnnnnntgcn nnnnngnnnn ncnnnnacnn nnttnnannc gnnnnnnnnn nnnntgtncn ngnncnnccn ncnccncnnn nancgnnnnn nnngtgcccn cgnagnnnan nnnnnnnnnn ntgnnntngn anncgtngaa nacannnnna gnnnnngcnn annnnntngn nnaaannant gnnngnncnn nnctcnnnnt tcgnncnngn cntgnnncga ncngnnnngn nnnncnnngn ganntngann nnnntnctng nnnaannncn nnnnnnnnna ngnnnnnnnt nnnnttngnn nnnnnnnatn nnnnaaanan nngannaann nnnnnnngnn gtnanngnnn nnnannnnnn ngnngnnngc cnttgcnnnc nnannnnnnt ancngnnnnn nnnnnttgnn cnnntnnnnn tntnnnnggn nnnannntnn nnnnntnnnn ncnnnnnnnn nncnnnnnnn ncnnnnnang gnnnnnnatc nnnnnnnngn annannnncn tnnnnnannn nngnngnnnn nnantnnnaa annnnnnnnn nnnnttnnnt nnnnncnnnn nnnnnngggn nnnnnnnnnn ntnnnnnnnc nnntnnnnnn gnna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSLPFSQDE RDLAAGGILT IDLAALRHNY SAIATRIAPT RTAAVVKADA YGLGASRVAP AFYEAGCRDF FVAHLGEAVA LKPFLKPDAT LYVLNGLQPG TEAACAREGI LPVLNSLEQV ENWAALATRL GKKLPALLQF DTGMSRLGLS AKEFDRLLEN VTLLSRIDIK FAISHLANGD EPGNAVNARQ LAKMTALLAR LPKLPAALAN SGGTFLGKTY YFDLARPGIA LYGIDPERQH DFSDKVAHEN KKPKHSILPV LTLSARVIQV RDVDKGATVG YGGTYVANGP MRIATIAVGY ADGLFRSLSN KGAAFFGDTR LPIIGRVSMD SITLDVTSLP EGTLKLGSLV ELIGPHQRLE DVARDCDTIP YEILTALGNR YARVYVYVNG GGTSTTA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctttacct ttttctcagg atgagcgcga tctcgcggct ggcggcatat tgaccattga tctcgcagcc ctgcgccaca attattcagc catcgcaacc cgcattgccc caacccgtac ggccgctgtg gtaaaggcag acgcctatgg cctgggcgca agccgggtcg caccggcttt ctacgaggcc ggttgccgcg atttcttcgt tgcacatctt ggcgaagcag tggcgctgaa gccctttttg aaacccgacg caacacttta tgtcctgaac ggccttcagc ccggaacgga agcagcctgt gcccgcgaag gcatcctgcc tgttctcaat tcgctggaac aggttgaaaa ctgggcagca ctcgccaccc ggctgggcaa aaaactgccc gccctgctcc agttcgatac cggcatgtct cgtcttggcc tgtcagccaa ggaatttgac cgccttcttg agaacgtcac gctcctgagc aggatcgaca tcaaattcgc catcagccat ctggcaaacg gcgacgagcc gggaaatgcg gtaaatgccc gtcagcttgc caagatgacg gccttgctcg ctcgcctgcc gaaactgccg gctgccttgg ccaattccgg cggcaccttc ctcggcaaga cctattattt cgatctcgcc cgccccggca tcgcgctata tggcatcgat ccggagaggc agcatgactt ttcggataag gtggcgcacg agaataagaa acccaagcat tcgatccttc ccgtcctcac cctttcggcg cgtgtcattc aggtgcgcga cgtcgacaag ggagcgacag ttggctatgg cggcacctat gtcgccaacg gcccgatgcg gattgcgacg attgcggtcg gctatgctga cggcttgttc cgttccttga gtaacaaggg cgcagccttt ttcggcgata cgcgcctgcc catcatcggg cgcgtatcga tggattcgat cacgctcgac gtcacctcat tgccggaggg aacgctgaaa ctcggcagcc ttgtggaatt gatcggcccg catcagcgcc tcgaagacgt ggcgcgggac tgcgacacta tcccctatga aattctgact gcgcttggca accgttatgc gcgcgtttac gtttacgtga atggcggtgg caccagcaca acagcataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for BrabA.00339.a.A1.PW31443

PURIFICATION DATe:	4/1/2011
CONCENTRATION:	21.68mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	38
VIAL VOLUME:	200µl
PERCENT IDENTITY:	8
PERCENT COVERAGE:	70

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTTTACCT TTTTCTCAGG ATGAGCGCGA TCTCGCGGCT GGCGGCATAT TGACCATTGA TCTCGCAGCC CTGCGCCACA ATTATTCAGC CATCGCAACC CGCATTGCCC CAACCCGTAC GGCCGCTGTG GTAAAGGCAG ACGCCTATGG CCTGGGCGCA AGCCGGGTCG CACCGGCTTT CTACGAGGCC GGTTGCCGCG ATTTCTTCGT TGCACATCTT GGCGAAGCAG TGGCGCTGAA GCCCTTTTTG AAACCCGACG CAACACTTTA TGTCCTGAAC GGCCTTCAGC CCGGAACGGA AGCAGCCTGT GCCCGCGAAG GCATCCTGCC TGTTCTCAAT TCGCTGGAAC AGGTTGAAAA CTGGGCAGCA CTCGCCACCC GGCTGGGCAA AAAACTGCCC GCCCTGCTCC AGTTCGATAC CGGCATGTCT CGTCTTGGCC TGTCAGCCAA GGAATTTGAC CGCCTTCTTG AGAACGTCAC GCTCCTGAGC AGGATCGACA TCAAATTCGC CATCAGCCAT CTGGCAAACG GCGACGAGCC GGGAAATGCG GTAAATGCCC GTCAGCTTGC CAAGATGACG GCCTTGCTCG CTCGCCTGCC GAAACTGCCG GCTGCCTTGG CCAATTCCGG CGGCACCTTC CTCGGCAAGA CCTATTATTT CGATCTCGCC CGCCCCGGCA TCGCGCTATA TGGCATCGAT CCGGAGAGGC AGCATGACTT TTCGGATAAG GTGGCGCACG AGAATAAGAA ACCCAAGCAT TCGATCCTTC CCGTCCTCAC CCTTTCGGCG CGTGTCATTC AGGTGCGCGA CGTCGACAAG GGAGCGACAG TTGGCTATGG CGGCACCTAT GTCGCCAACG GCCCGATGCG GATTGCGACG ATTGCGGTCG GCTATGCTGA CGGCTTGTTC CGTTCCTTGA GTAACAAGGG CGCAGCCTTT TTCGGCGATA CGCGCCTGCC CATCATCGGG CGCGTATCGA TGGATTCGAT CACGCTCGAC GTCACCTCAT TGCCGGAGGG AACGCTGAAA CTCGGCAGCC TTGTGGAATT GATCGGCCCG CATCAGCGCC TCGAAGACGT GGCGCGGGAC TGCGACACTA TCCCCTATGA AATTCTGACT GCGCTTGGCA ACCGTTATGC GCGCGTTTAC GTTTACGTGA ATGGCGGTGG CACCAGCACA ACAGCATAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA