BrabA.00323.a

ZINC PROTEASE (EC 3.4.99.-)

CENTER ID: BrabA.00323.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00323.a.B1.GE37715 Full length( BrabA.00323.a ) 1 250
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel86222_2487

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKSLQSISRD DLANFHRKNF ARDKLTVGVV GAINAKDLGV MLDRIFGDLP ASAELVPVPD AKLALGTTTS LNFDMPQTSI SFVYPAIPRK DPEFFAAYLM NHILGGGFTS RLYNEVREKR GLAYSVSSSM VMHDHVSELM ISTATRPDKA QDSLKIIREQ VAAMANDGPT EEELAAAKSF LKGSYAVNNL DSSGAIANTL VSLQEAGLPS DYIDKRSELI DAVTLDQVKA IAWKLLQAEP AILIYGPAQS
NT Sequence
GTGAAATCCC TGCAATCGAT CAGCCGCGAT GATCTTGCGA ATTTCCATCG CAAGAATTTC GCCCGCGACA AGCTGACCGT TGGCGTTGTG GGTGCCATCA ACGCCAAGGA TCTGGGCGTC ATGCTCGACA GGATTTTTGG CGACCTGCCC GCCTCGGCGG AACTGGTTCC GGTGCCGGAT GCGAAGCTTG CGCTTGGCAC CACCACCAGC CTGAATTTCG ATATGCCGCA GACGTCGATC AGCTTCGTCT ACCCGGCCAT TCCGCGCAAA GACCCTGAGT TTTTTGCCGC CTATCTGATG AACCATATTC TGGGCGGCGG TTTCACCTCG CGTCTTTATA ACGAAGTGCG TGAAAAGCGC GGCCTTGCCT ATTCGGTGTC CTCGTCCATG GTCATGCACG ACCATGTTTC CGAACTGATG ATTTCCACCG CCACCCGGCC TGACAAGGCC CAGGACTCAT TGAAAATCAT TCGTGAGCAG GTTGCCGCCA TGGCCAATGA CGGCCCGACC GAGGAAGAGC TTGCCGCAGC CAAGAGCTTT CTCAAAGGCT CCTATGCGGT GAACAATCTC GATTCGTCGG GAGCTATTGC CAATACGCTG GTGAGTTTGC AGGAAGCCGG GCTTCCAAGC GATTATATCG ACAAGCGTTC CGAACTCATC GATGCTGTCA CGCTGGATCA GGTCAAGGCC ATTGCCTGGA AACTGCTTCA GGCCGAGCCT GCGATCCTGA TCTATGGACC GGCGCAAAGT
Details for BrabA.00323.a.B1.GE37715
HARVESTED ON: 2/5/2014
SEQUENCED ON: 2/6/2014
EXPECTED MW: 28kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV KSLQSISRDD LANFHRKNFA RDKLTVGVVG AINAKDLGVM LDRIFGDLPA SAELVPVPDA KLALGTTTSL NFDMPQTSIS FVYPAIPRKD PEFFAAYLMN HILGGGFTSR LYNEVREKRG LAYSVSSSMV MHDHVSELMI STATRPDKAQ DSLKIIREQV AAMANDGPTE EELAAAKSFL KGSYAVNNLD SSGAIANTLV SLQEAGLPSD YIDKRSELID AVTLDQVKAI AWKLLQAEPA ILIYGPAQS
Validated NT Sequence
annnctctag atattttgtt taactttaag aaggagatat accatggctc accaccacca ccaccatatg gtgaaatccc tgcaatcgat cagccgcgat gatcttgcga atttccatcg caagaatttc gcccgcgaca agctgaccgt tggcgttgtg ggtgccatca acgccaagga tctgggcgtc atgctcgaca ggatttttgg cgacctgccc gcctcggcgg aactggttcc ggtgccggat gcgaagcttg cgcttggcac caccaccagc ctgaatttcg atatgccgca gacgtcgatc agcttcgtct acccggccat tccgcgcaaa gaccctgagt tttttgccgc ctatctgatg aaccatattc tgggcggcgg tttcacctcg cgtctttata acgaagtgcg tgaaaagcgc ggccttgcct attcggtgtc ctcgtccatg gtcatgcacg accatgtttc cgaactgatg atttccaccg ccacccggcc tgacaaggcc caggactcat tgaaaatcat tcgtgagcag gttgccgcca tggccaatga cggcccgacc gaggaagagc ttgccgcagc caagagcttt ctcaaaggct cctatgcggt gaacaatctc gattcgtcgg gagctattgc caatacgctg gtgagtttgc aggaagccgg gcttccaagc gattatatcg acaagcgttc cgaactcatc gatgctgtca cgctggatca ggtcaaggcc attgcctgga aactgcttca ggccgagcct gcgatcctga tctatggacc ggcgcaaagt taagtgagta agataggatc ctgnannnac cccnncacan agagaanaan nncggnnnnn nnnnnnnnca tanntttang gaaacggctn gcnatacgct gggcaggttt gnnntanact gcncgcgaac tgattaccnn ctnntcgatt cgananggtg tgtaccacgc gacnagagca taatctnnga tctngnnann ngccantcng aaanncgnnc gnagctaatt cncnnggaac ctntcnggag annccgnnct tnccgnnnat acnagtannn gnntnntnnn naaannnnnc ta
Expected Protein Sequence
MAHHHHHHMK SLQSISRDDL ANFHRKNFAR DKLTVGVVGA INAKDLGVML DRIFGDLPAS AELVPVPDAK LALGTTTSLN FDMPQTSISF VYPAIPRKDP EFFAAYLMNH ILGGGFTSRL YNEVREKRGL AYSVSSSMVM HDHVSELMIS TATRPDKAQD SLKIIREQVA AMANDGPTEE ELAAAKSFLK GSYAVNNLDS SGAIANTLVS LQEAGLPSDY IDKRSELIDA VTLDQVKAIA WKLLQAEPAI LIYGPAQS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaaat ccctgcaatc gatcagccgc gatgatcttg cgaatttcca tcgcaagaat ttcgcccgcg acaagctgac cgttggcgtt gtgggtgcca tcaacgccaa ggatctgggc gtcatgctcg acaggatttt tggcgacctg cccgcctcgg cggaactggt tccggtgccg gatgcgaagc ttgcgcttgg caccaccacc agcctgaatt tcgatatgcc gcagacgtcg atcagcttcg tctacccggc cattccgcgc aaagaccctg agttttttgc cgcctatctg atgaaccata ttctgggcgg cggtttcacc tcgcgtcttt ataacgaagt gcgtgaaaag cgcggccttg cctattcggt gtcctcgtcc atggtcatgc acgaccatgt ttccgaactg atgatttcca ccgccacccg gcctgacaag gcccaggact cattgaaaat cattcgtgag caggttgccg ccatggccaa tgacggcccg accgaggaag agcttgccgc agccaagagc tttctcaaag gctcctatgc ggtgaacaat ctcgattcgt cgggagctat tgccaatacg ctggtgagtt tgcaggaagc cgggcttcca agcgattata tcgacaagcg ttccgaactc atcgatgctg tcacgctgga tcaggtcaag gccattgcct ggaaactgct tcaggccgag cctgcgatcc tgatctatgg accggcgcaa agttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat