BrabA.00276.a

Triosephosphate isomerase 2

CENTER ID: BrabA.00276.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00276.a.A1.GE31544 Full length( BrabA.00276.a ) 1 256
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00276.a.A1.PW31442 Full length( BrabA.00276.a ) 1 256

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.2660
RefSeq: YP_418574.1
UniProt: Q2YIQ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTKFWIGTSW KMNKTLAEAR LFAEALKAAD AGRSPDIQRF VIPPFTAVRE VKEILSGTSV KVGAQNMHWA DQGAWTGEIS PLMLKDCNLD IVELGHSERR EHFGETNETV GLKVEAAVRH GLIPLICIGE TLEDRESGRA AAVLEEEVRG ALSKLSEAQK QAEILFAYEP VWAIGENGIP ASADYADARQ AEIIAVAQSV LARRVPCLYG GSVNPGNCEE LIACPHIDGL FIGRSAWNVE GYLDILARCA TKVQAN
NT Sequence
ATGACCAAAT TCTGGATTGG CACAAGCTGG AAAATGAACA AGACGCTGGC CGAAGCCCGC CTTTTCGCGG AAGCGCTGAA AGCCGCCGAT GCAGGCCGCT CGCCAGACAT CCAGCGTTTT GTCATCCCGC CTTTCACCGC CGTGCGTGAG GTGAAGGAAA TCCTCTCCGG CACATCCGTG AAGGTTGGCG CGCAGAACAT GCACTGGGCC GATCAGGGTG CATGGACCGG TGAAATCTCC CCGCTGATGC TGAAAGATTG CAATCTCGAC ATTGTTGAAC TGGGCCATTC GGAGCGCCGC GAACATTTCG GCGAGACCAA CGAGACGGTC GGCTTGAAAG TGGAAGCCGC CGTGCGCCAC GGCCTGATCC CGCTCATCTG CATCGGTGAA ACGCTGGAAG ACCGTGAAAG CGGACGCGCA GCCGCGGTGC TTGAAGAAGA AGTGCGCGGC GCGCTTTCCA AGCTTTCCGA GGCGCAGAAG CAGGCCGAAA TCCTCTTCGC CTATGAGCCA GTCTGGGCCA TCGGCGAAAA CGGCATTCCC GCTTCCGCCG ACTATGCCGA TGCGCGACAG GCCGAAATCA TCGCGGTGGC ACAAAGCGTG CTCGCTCGCC GCGTGCCGTG CCTTTATGGC GGCTCGGTCA ATCCGGGCAA TTGCGAAGAA CTGATCGCCT GCCCGCATAT CGATGGGCTT TTCATCGGCC GTTCGGCGTG GAACGTCGAA GGCTATCTCG ACATTCTGGC CAGGTGCGCC ACCAAGGTTC AAGCGAAT
Details for BrabA.00276.a.A1.GE31544
HARVESTED ON: 2/1/2011
SEQUENCED ON: 2/8/2011
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTKFWIGTS WKMNKTLAEA RLFAEALKAA DAGRSPDIQR FVIPPFTAVR EVKEILSGTS VKVGAQNMHW ADQGAWTGEI SPLMLKGCNL DIVELGHSER REHFGETNET VGLKVEAAVR HGLIPLICIG ETLEDRESGR AAAVLEEEVR GALSKLSEAQ KQAEILFAYE PVWAIGENGI PASADYADAR QAEIIAVAQS VLARRVPCLY GGSVNPGNCE ELIACPHIDG LFIGRSAWNV EGYLDILARC ATKVQAN
Validated NT Sequence
aattcgcttg aaccttggtg gcgcacctgg ccagaatgtc gagatagcct tcgacgttcc acgccgaacg gccgatgaaa agcccatcga tatgcgggca ggcgatcagt tcttcgcaat tgcccggatt gaccgagccg ccataaaggc acggcacgcg gcgagcgagc acgctttgtg ccaccgcgat gatttcggcc tgtcgcgcat cggcatagtc ggcggaggcg ggaatgccgt tttcgccgat ggcccagact ggctcatagg cgaagaggat ttcggcctgc ttctgcgcct cggaaagctt ggaaagcgcg ccgcgcactt cttcttcaag caccgcggct gcgcgtccgc tttcacggtc ttccagcgtt tcaccgatgc agatgagcgg gatcaggccg tggcgcacgg cggcttccac tttcaagccg accgtctcgt tggtctcgcc gaaatgttcg cggcgctccg aatggcccag ttcaacaatg tcgagattgc aacctttcag catcagcggg gagatttcac cggtccatgc accctgatcg gcccagtgca tgttctgcgc gccaaccttc acggatgtgc cggagaggat ttccttcacc tcacgcacgg cggtgaaagg cgggatgaca aaacgctgga tgtctggcga gcggcctgca tcggcggctt tcagcgcttc cgcgaaaagg cgggcttcgg ccagcgtctt gttcattttc cagcttgtgc caatccagaa tttggtcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTKFWIGTS WKMNKTLAEA RLFAEALKAA DAGRSPDIQR FVIPPFTAVR EVKEILSGTS VKVGAQNMHW ADQGAWTGEI SPLMLKDCNL DIVELGHSER REHFGETNET VGLKVEAAVR HGLIPLICIG ETLEDRESGR AAAVLEEEVR GALSKLSEAQ KQAEILFAYE PVWAIGENGI PASADYADAR QAEIIAVAQS VLARRVPCLY GGSVNPGNCE ELIACPHIDG LFIGRSAWNV EGYLDILARC ATKVQAN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccaaattc tggattggca caagctggaa aatgaacaag acgctggccg aagcccgcct tttcgcggaa gcgctgaaag ccgccgatgc aggccgctcg ccagacatcc agcgttttgt catcccgcct ttcaccgccg tgcgtgaggt gaaggaaatc ctctccggca catccgtgaa ggttggcgcg cagaacatgc actgggccga tcagggtgca tggaccggtg aaatctcccc gctgatgctg aaagattgca atctcgacat tgttgaactg ggccattcgg agcgccgcga acatttcggc gagaccaacg agacggtcgg cttgaaagtg gaagccgccg tgcgccacgg cctgatcccg ctcatctgca tcggtgaaac gctggaagac cgtgaaagcg gacgcgcagc cgcggtgctt gaagaagaag tgcgcggcgc gctttccaag ctttccgagg cgcagaagca ggccgaaatc ctcttcgcct atgagccagt ctgggccatc ggcgaaaacg gcattcccgc ttccgccgac tatgccgatg cgcgacaggc cgaaatcatc gcggtggcac aaagcgtgct cgctcgccgc gtgccgtgcc tttatggcgg ctcggtcaat ccgggcaatt gcgaagaact gatcgcctgc ccgcatatcg atgggctttt catcggccgt tcggcgtgga acgtcgaagg ctatctcgac attctggcca ggtgcgccac caaggttcaa gcgaattaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BrabA.00276.a.A1.PW31442
PURIFICATION DATe: 4/1/2011
CONCENTRATION: 20.08mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 36
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTKFWIGTS WKMNKTLAEA RLFAEALKAA DAGRSPDIQR FVIPPFTAVR EVKEILSGTS VKVGAQNMHW ADQGAWTGEI SPLMLKGCNL DIVELGHSER REHFGETNET VGLKVEAAVR HGLIPLICIG ETLEDRESGR AAAVLEEEVR GALSKLSEAQ KQAEILFAYE PVWAIGENGI PASADYADAR QAEIIAVAQS VLARRVPCLY GGSVNPGNCE ELIACPHIDG LFIGRSAWNV EGYLDILARC ATKVQAN
Validated NT Sequence
aattcgcttg aaccttggtg gcgcacctgg ccagaatgtc gagatagcct tcgacgttcc acgccgaacg gccgatgaaa agcccatcga tatgcgggca ggcgatcagt tcttcgcaat tgcccggatt gaccgagccg ccataaaggc acggcacgcg gcgagcgagc acgctttgtg ccaccgcgat gatttcggcc tgtcgcgcat cggcatagtc ggcggaggcg ggaatgccgt tttcgccgat ggcccagact ggctcatagg cgaagaggat ttcggcctgc ttctgcgcct cggaaagctt ggaaagcgcg ccgcgcactt cttcttcaag caccgcggct gcgcgtccgc tttcacggtc ttccagcgtt tcaccgatgc agatgagcgg gatcaggccg tggcgcacgg cggcttccac tttcaagccg accgtctcgt tggtctcgcc gaaatgttcg cggcgctccg aatggcccag ttcaacaatg tcgagattgc aacctttcag catcagcggg gagatttcac cggtccatgc accctgatcg gcccagtgca tgttctgcgc gccaaccttc acggatgtgc cggagaggat ttccttcacc tcacgcacgg cggtgaaagg cgggatgaca aaacgctgga tgtctggcga gcggcctgca tcggcggctt tcagcgcttc cgcgaaaagg cgggcttcgg ccagcgtctt gttcattttc cagcttgtgc caatccagaa tttggtcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTKFWIGTS WKMNKTLAEA RLFAEALKAA DAGRSPDIQR FVIPPFTAVR EVKEILSGTS VKVGAQNMHW ADQGAWTGEI SPLMLKDCNL DIVELGHSER REHFGETNET VGLKVEAAVR HGLIPLICIG ETLEDRESGR AAAVLEEEVR GALSKLSEAQ KQAEILFAYE PVWAIGENGI PASADYADAR QAEIIAVAQS VLARRVPCLY GGSVNPGNCE ELIACPHIDG LFIGRSAWNV EGYLDILARC ATKVQAN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCAAATTC TGGATTGGCA CAAGCTGGAA AATGAACAAG ACGCTGGCCG AAGCCCGCCT TTTCGCGGAA GCGCTGAAAG CCGCCGATGC AGGCCGCTCG CCAGACATCC AGCGTTTTGT CATCCCGCCT TTCACCGCCG TGCGTGAGGT GAAGGAAATC CTCTCCGGCA CATCCGTGAA GGTTGGCGCG CAGAACATGC ACTGGGCCGA TCAGGGTGCA TGGACCGGTG AAATCTCCCC GCTGATGCTG AAAGATTGCA ATCTCGACAT TGTTGAACTG GGCCATTCGG AGCGCCGCGA ACATTTCGGC GAGACCAACG AGACGGTCGG CTTGAAAGTG GAAGCCGCCG TGCGCCACGG CCTGATCCCG CTCATCTGCA TCGGTGAAAC GCTGGAAGAC CGTGAAAGCG GACGCGCAGC CGCGGTGCTT GAAGAAGAAG TGCGCGGCGC GCTTTCCAAG CTTTCCGAGG CGCAGAAGCA GGCCGAAATC CTCTTCGCCT ATGAGCCAGT CTGGGCCATC GGCGAAAACG GCATTCCCGC TTCCGCCGAC TATGCCGATG CGCGACAGGC CGAAATCATC GCGGTGGCAC AAAGCGTGCT CGCTCGCCGC GTGCCGTGCC TTTATGGCGG CTCGGTCAAT CCGGGCAATT GCGAAGAACT GATCGCCTGC CCGCATATCG ATGGGCTTTT CATCGGCCGT TCGGCGTGGA ACGTCGAAGG CTATCTCGAC ATTCTGGCCA GGTGCGCCAC CAAGGTTCAA GCGAATTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA