BrabA.00232.a

Xylulose kinase (EC 2.7.1.17)

CENTER ID: BrabA.00232.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00232.a.B1.GE37714 Full length( BrabA.00232.a ) 1 483
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel86222_0598
BV-BRC: fig|359391.4.peg.598
RefSeq: YP_414022.1
UniProt: Q2YMQ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYLGLDLGTS GVKALLIDEA QNPVGAAHGE LDVSRPHPGW SEQDPAQWIK ACRTAIEALR AAHPKEFSAI TGIGLSGQMH GATLLDAEDR VLRPCILWND TRSYREAAEL DADPAFRAIT GNIVFPSFTA PKLVWIARNE ADIFARIRKV LLPKDYLRLW LAGEYISDMS DSAGTSWLDT GARRWSAELL AKTGLGEGQM PQLVEGSEAA GCLRAELAAE WSLTASVIVA GGAGDNAASA CGMGTVKPGH AFVSLGTSGV LFAANGAYQP KPESAVHAFC HALPRTWHQM GVILSAASAL EWYSKIVGAT PQSLDRELGE TLKAPGSVTF LPYLSGERTP YNDAKIRGSF CGLEHEADRS ALTQAVLEGV AFAIRDNRLA LQSAGTEITS LTAVGGGSRS TYWLKAIATA LNVPIALPEE GDFGAAFGAA RLGLIAATGA DPFTICTPPQ TARTIEPEQA LLSAYDEAYQ RYHALYPALH ALD
NT Sequence
ATGTATCTGG GTCTCGATTT GGGAACATCC GGCGTCAAGG CGCTTTTGAT CGATGAGGCG CAGAACCCGG TCGGCGCGGC CCATGGCGAA CTGGACGTTT CGCGCCCGCA TCCCGGCTGG AGCGAACAGG ATCCTGCGCA GTGGATCAAA GCCTGCCGTA CGGCCATAGA AGCCTTGCGC GCGGCGCACC CGAAGGAATT TTCCGCCATT ACCGGTATCG GCCTTTCCGG GCAGATGCAT GGCGCAACCC TGCTGGACGC CGAGGACCGC GTATTGCGCC CCTGCATTCT CTGGAACGAT ACGCGCAGCT ATCGCGAAGC AGCCGAACTG GATGCCGACC CCGCATTTCG CGCCATCACC GGCAATATCG TCTTTCCCAG CTTCACCGCA CCGAAACTTG TCTGGATCGC ACGCAACGAA GCCGATATTT TCGCCCGCAT CCGCAAGGTT TTACTGCCGA AAGATTATCT GCGTCTCTGG CTCGCCGGAG AATATATCTC CGACATGTCG GATTCGGCGG GCACAAGCTG GCTCGATACA GGTGCGCGCC GCTGGTCGGC AGAGCTTCTG GCCAAGACCG GCCTGGGCGA AGGGCAGATG CCGCAACTGG TCGAGGGGAG CGAGGCTGCG GGATGTCTGC GCGCCGAGCT TGCCGCCGAA TGGAGCCTCA CCGCATCGGT AATCGTGGCG GGCGGCGCTG GCGACAATGC GGCTTCCGCC TGCGGCATGG GCACGGTCAA GCCCGGCCAT GCCTTCGTTT CACTTGGCAC TTCGGGCGTG CTTTTCGCTG CCAATGGCGC TTACCAGCCA AAACCGGAAA GTGCGGTCCA TGCCTTCTGC CACGCCCTGC CCCGCACATG GCACCAGATG GGCGTTATCC TGTCTGCGGC AAGCGCGCTT GAATGGTATT CAAAAATCGT TGGCGCAACC CCGCAATCGC TCGATCGGGA GCTGGGCGAA ACCTTGAAAG CACCGGGCAG CGTCACCTTC CTGCCCTATC TTTCAGGCGA GCGCACGCCC TATAATGACG CGAAAATCCG CGGCTCCTTC TGCGGGCTGG AACATGAAGC CGACCGAAGT GCCCTCACCC AGGCCGTACT GGAAGGCGTC GCCTTTGCCA TTCGCGACAA TCGTCTTGCC CTGCAATCGG CTGGCACCGA GATTACCTCG CTCACCGCAG TCGGTGGCGG ATCACGCTCC ACCTATTGGC TGAAAGCCAT TGCAACAGCA TTGAATGTGC CGATTGCACT GCCCGAAGAA GGCGATTTCG GCGCAGCCTT TGGCGCGGCC CGCCTCGGCC TCATCGCGGC AACCGGGGCC GATCCCTTCA CCATCTGCAC CCCGCCGCAG ACCGCGCGCA CCATTGAGCC GGAACAGGCG CTGCTTTCAG CTTATGACGA AGCCTATCAG CGTTATCACG CGCTTTATCC GGCGCTCCAT GCACTTGAC
Details for BrabA.00232.a.B1.GE37714
HARVESTED ON: 2/5/2014
SEQUENCED ON: 2/6/2014
EXPECTED MW: 52kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 92
PERCENT COVERAGE: 76
Validated AA Sequence
MAHHHHHHHM YLGLDLGTSG VKALLIDEAQ NPVGAAHGEL DVSRPHPGWS EQDPAQWIKA CRTAIEALRA AHPKEFSAIT GIGLSGQMHG ATLLDAEDRV LRPCILWNDT RSYREAAELD ADPAFRAITG NIVFPSFTAP KLVWIARNEA DIFARIRKVL LPKDYLRLWL AGEYISDMSD SAGTSWLDTG ARRWSAELLA KTGLGEGQMP QLVEGSEAAG CLRAELAAEW SLTASVIVAG GAGDNAASAC GMGTVKPGHA FVSLGTSGVL FAANGAYQPK PESAVHAFCH ALPRTWHQMG VILSAASALE WYSKIVGATP QSLDRELGEI XKAPGSVTXX PYLSXXXTPX XDXEIXXSXX XGWXMKPTXX AXXX
Validated NT Sequence
cnctagaatn attttgttta actttaagaa ggagatatac catggctcac caccaccacc accaccatat gtatctgggt ctcgatttgg gaacatccgg cgtcaaggcg cttttgatcg atgaggcgca gaacccggtc ggcgcggccc atggcgaact ggacgtttcg cgcccgcatc ccggctggag cgaacaggat cctgcgcagt ggatcaaagc ctgccgtacg gccatagaag ccttgcgcgc ggcgcacccg aaggaatttt ccgccattac cggtatcggc ctttccgggc agatgcatgg cgcaaccctg ctggacgccg aggaccgcgt attgcgcccc tgcattctct ggaacgatac gcgcagctat cgcgaagcag ccgaactgga tgccgacccc gcatttcgcg ccatcaccgg caatatcgtc tttcccagct tcaccgcacc gaaacttgtc tggatcgcac gcaacgaagc cgatattttc gcccgcatcc gcaaggtttt actgccgaaa gattatctgc gtctctggct cgccggagaa tatatctccg acatgtcgga ttcggcgggc acaagctggc tcgatacagg tgcgcgccgc tggtcggcag agcttctggc caagaccggc ctgggcgaag ggcagatgcc gcaactggtc gaggggagcg aggctgcggg atgtctgcgc gccgagcttg ccgccgaatg gagcctcacc gcatcggtaa tcgtggcggg cggcgctggc gacaatgcgg cttccgcctg cggcatgggc acggtcaagc ccggccatgc cttcgtttca cttggcactt cgggcgtgct tttcgctgcc aatggcgctt accagccaaa accggaaagt gcggtccatg ccttctgcca cgccctgccc cgcacatggc accagatggg cgttatcctg tctgcggcaa gcgcgcttga atggtattca aaaatcgttg gcgcaacccc gcaatcgctc gatcgggagc tgggcgaaat cnngaaagca ccgggcagcg tcaccnttcn gccctatctt tcagnnngcg nnacgcccta nnatgaccnc gaaatccnnn gctccttnnn nncgggctgg aanatgaagc cnaccnaaan tgccntcncc cang
Expected Protein Sequence
MAHHHHHHMY LGLDLGTSGV KALLIDEAQN PVGAAHGELD VSRPHPGWSE QDPAQWIKAC RTAIEALRAA HPKEFSAITG IGLSGQMHGA TLLDAEDRVL RPCILWNDTR SYREAAELDA DPAFRAITGN IVFPSFTAPK LVWIARNEAD IFARIRKVLL PKDYLRLWLA GEYISDMSDS AGTSWLDTGA RRWSAELLAK TGLGEGQMPQ LVEGSEAAGC LRAELAAEWS LTASVIVAGG AGDNAASACG MGTVKPGHAF VSLGTSGVLF AANGAYQPKP ESAVHAFCHA LPRTWHQMGV ILSAASALEW YSKIVGATPQ SLDRELGETL KAPGSVTFLP YLSGERTPYN DAKIRGSFCG LEHEADRSAL TQAVLEGVAF AIRDNRLALQ SAGTEITSLT AVGGGSRSTY WLKAIATALN VPIALPEEGD FGAAFGAARL GLIAATGADP FTICTPPQTA RTIEPEQALL SAYDEAYQRY HALYPALHAL D
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtatc tgggtctcga tttgggaaca tccggcgtca aggcgctttt gatcgatgag gcgcagaacc cggtcggcgc ggcccatggc gaactggacg tttcgcgccc gcatcccggc tggagcgaac aggatcctgc gcagtggatc aaagcctgcc gtacggccat agaagccttg cgcgcggcgc acccgaagga attttccgcc attaccggta tcggcctttc cgggcagatg catggcgcaa ccctgctgga cgccgaggac cgcgtattgc gcccctgcat tctctggaac gatacgcgca gctatcgcga agcagccgaa ctggatgccg accccgcatt tcgcgccatc accggcaata tcgtctttcc cagcttcacc gcaccgaaac ttgtctggat cgcacgcaac gaagccgata ttttcgcccg catccgcaag gttttactgc cgaaagatta tctgcgtctc tggctcgccg gagaatatat ctccgacatg tcggattcgg cgggcacaag ctggctcgat acaggtgcgc gccgctggtc ggcagagctt ctggccaaga ccggcctggg cgaagggcag atgccgcaac tggtcgaggg gagcgaggct gcgggatgtc tgcgcgccga gcttgccgcc gaatggagcc tcaccgcatc ggtaatcgtg gcgggcggcg ctggcgacaa tgcggcttcc gcctgcggca tgggcacggt caagcccggc catgccttcg tttcacttgg cacttcgggc gtgcttttcg ctgccaatgg cgcttaccag ccaaaaccgg aaagtgcggt ccatgccttc tgccacgccc tgccccgcac atggcaccag atgggcgtta tcctgtctgc ggcaagcgcg cttgaatggt attcaaaaat cgttggcgca accccgcaat cgctcgatcg ggagctgggc gaaaccttga aagcaccggg cagcgtcacc ttcctgccct atctttcagg cgagcgcacg ccctataatg acgcgaaaat ccgcggctcc ttctgcgggc tggaacatga agccgaccga agtgccctca cccaggccgt actggaaggc gtcgcctttg ccattcgcga caatcgtctt gccctgcaat cggctggcac cgagattacc tcgctcaccg cagtcggtgg cggatcacgc tccacctatt ggctgaaagc cattgcaaca gcattgaatg tgccgattgc actgcccgaa gaaggcgatt tcggcgcagc ctttggcgcg gcccgcctcg gcctcatcgc ggcaaccggg gccgatccct tcaccatctg caccccgccg cagaccgcgc gcaccattga gccggaacag gcgctgcttt cagcttatga cgaagcctat cagcgttatc acgcgcttta tccggcgctc catgcacttg actgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat