BrabA.00164.a

arginyl-tRNA synthetase

CENTER ID:	BrabA.00164.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.00164.a.A1.GE25582	Full length( BrabA.00164.a )		1	585

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.00164.a.A1.PW25627	Full length( BrabA.00164.a )		1	585

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126752
PATRIC ID:	fig\|359391.4.peg.931
RefSeq:	YP_414321.1
UniProt:	Q2YNJ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNIFADFDAR IKKTFQDIDL KPKDGGELDL SRIGVEPPRD ASHGDIATNA AMVLSKAVGQ NPRELAARIA EALKADEDVE SVDVAGPGFI NLRLKASYWQ RELLVMLNEG TDFGRSRLGA GKKVNVEYVS ANPTGPMHVG HCRGAVVGDV LANLLKFAGY DVVKEYYIND AGAQIDVLAR SVMLRYREAL GESIGEIPAG LYPGDYLVRV GQELAGEFGT KLLEMPEAEA LAIVKDRTID AMMAMIRADL DALNVHHDVF YSERKLHVDH ARAIRNAIND LTLKGHVYKG KLPPPKGRLP EDWEDREQTL FRSTEVGDDI DRPLMKSDGS FTYFAGDVTY FKDKYDRGFN EMIYVLGADH GGYVKRLEAV ARAVSDGKAK LTVLLCQLVK LFRNGEPVRM SKRAGEFITL RDVVDEVGRD PVRFMMLYRK NDAPLDFDFA KVTEQSKDNP VFYVQYASAR CHSVFRQAAD QLGLVDLDRV AMGSHFEKLT DESEIALVRK LAEYPRLIES AAIHQEPHRL AFYLYDLASS FHSQWNRGAE NPDLRFIKVN DPDLSLARLG LVQVVSDVLT SGLTIIGADA PTEMR

NT Sequence

ATGAATATCT TTGCAGATTT CGATGCACGT ATTAAAAAAA CGTTTCAAGA TATTGATCTG AAACCAAAAG ATGGTGGTGA ACTTGACCTT TCACGCATAG GTGTCGAGCC CCCGCGCGAT GCTTCGCATG GCGATATCGC GACCAATGCC GCAATGGTGC TTTCCAAGGC GGTCGGCCAG AACCCGCGCG AGCTTGCTGC CCGCATTGCC GAGGCGCTGA AGGCCGATGA GGATGTCGAA TCCGTCGATG TCGCCGGTCC GGGCTTCATC AATCTGCGCC TCAAGGCCAG CTATTGGCAG CGCGAGCTTT TGGTGATGCT GAACGAGGGA ACGGATTTTG GCCGCTCCAG GCTTGGCGCG GGCAAGAAGG TCAATGTCGA ATATGTATCG GCCAACCCTA CGGGCCCGAT GCATGTCGGC CATTGCCGGG GCGCGGTTGT GGGTGATGTG CTGGCGAACC TGCTGAAATT CGCGGGCTAT GACGTCGTCA AGGAATATTA CATCAACGAT GCGGGCGCGC AGATCGATGT TCTCGCGCGT TCTGTGATGC TGCGCTATCG TGAGGCACTG GGCGAAAGCA TCGGCGAGAT TCCGGCGGGG CTTTACCCCG GTGATTATCT CGTGCGGGTC GGGCAGGAGC TTGCCGGTGA GTTTGGCACA AAACTTCTGG AAATGCCTGA AGCCGAAGCA CTGGCCATCG TGAAGGACCG CACGATCGAC GCCATGATGG CAATGATCCG CGCTGATCTC GACGCGCTGA ACGTGCATCA TGATGTCTTC TATTCCGAGC GCAAGCTACA TGTCGACCAT GCGCGCGCGA TCCGTAACGC GATCAATGAT CTGACGCTCA AGGGCCATGT TTATAAGGGC AAGCTGCCGC CTCCGAAGGG GCGGTTGCCG GAAGATTGGG AAGACCGTGA GCAGACGCTG TTCCGTTCTA CGGAAGTGGG CGACGATATT GACCGTCCGC TGATGAAGTC CGATGGCTCG TTCACCTATT TTGCGGGTGA CGTGACCTAT TTCAAGGACA AGTACGACCG CGGCTTCAAT GAGATGATCT ATGTGCTCGG CGCGGATCAT GGCGGTTATG TCAAGCGTCT GGAAGCCGTT GCGCGTGCCG TTTCCGATGG CAAGGCCAAA TTGACCGTGC TCCTGTGCCA GCTCGTGAAG CTGTTCCGCA ATGGCGAGCC GGTGCGCATG TCCAAGCGGG CAGGCGAGTT CATTACGCTG CGCGATGTGG TGGACGAGGT CGGTCGCGAT CCGGTCCGCT TCATGATGCT TTACCGGAAG AACGATGCGC CATTGGACTT CGACTTCGCC AAGGTGACGG AGCAGTCCAA GGACAATCCG GTTTTCTATG TGCAATATGC TTCCGCGCGC TGCCATTCGG TTTTCCGTCA GGCTGCGGAC CAGCTTGGGC TTGTCGATCT TGACCGCGTG GCCATGGGGT CGCATTTTGA AAAGCTGACC GACGAGAGCG AAATTGCGCT CGTTCGCAAG CTTGCGGAAT ACCCGCGCCT GATCGAATCG GCAGCCATTC ATCAGGAGCC GCATCGCCTG GCTTTCTACC TCTACGACCT CGCCAGTTCG TTCCATTCGC AATGGAACAG AGGCGCGGAA AATCCGGACT TACGTTTTAT TAAGGTTAAC GATCCAGACT TGTCGCTGGC CAGGCTAGGG CTGGTGCAGG TTGTTTCCGA TGTGCTGACG TCAGGATTGA CGATTATCGG CGCGGATGCT CCTACGGAGA TGCGC

Details for BrabA.00164.a.A1.GE25582

HARVESTED ON:	9/10/2008
SEQUENCED ON:	7/28/2010
EXPECTED MW:	67kDa
OBSERVED MW:	67kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	89
PERCENT COVERAGE:	37

Validated AA Sequence

MVLSKAVGQN PRELAXXXAE ALKADEDVES VDVAGPGFXX LRLKASYXXR ELLVMLNEGX XFGRSRLGAX XKVNVEYVSA NPTGPMHVGH CRGAVVGDVL ANLLKFAGYD VVKEYYINDA GAQIDVLARS VMLRYREALG ESIGEIPAGL YPGDYLVRVG QELAGEFGTK LLEMXEAEAL XIVXDRTIDA MMAMIRADLD ALNVHHDVFY SSASYMSTMR ARSVTRSMX

Validated NT Sequence

cnanntgnng nnnngnngna nnnnnanaan nnnannncnn gganntnnng annnncnccn ttananggnn nnnctggnnn ngcanaccnn gnnnctgggn nnangnngnt cnntgnnnnn nncannnnnn tgannngnnt gctgncnccn nanannnatn nnnancanna agatggtggt gaacttgacc tttcacgcat aggtgtcgag cccccacgcg atgcttcgca tggcgatatc gcgaccaatg ccgcaatggt gctttccaag gcggtcggcc agaacccgcg cgagcttgct gnccnnnttg ccgaggcgct gaaggccgat gaggatgtcg aatccgtcga tgtcgccggt ccgggcttcn tcnatctgcg cctcaaggcc agctattgnn agcgcgagct tttggtgatg ctgaacgagg gnnnnnattt tggccgctcc aggcttggcg cgnnnnagaa ggtcaatgtc gaatatgtat cggccaaccc tacgggcccg atgcatgtcg gccattgccg gggcgcggtt gtgggtgatg tgctggcgaa cctgctgaaa ttcgcgggct atgacgtcgt caaggaatat tacatcaacg atgcgggcgc gcagatcgat gttctcgcgc gctctgtgat gctgcgctat cgtgaggcac tgggcgaaag catcggcgag attccggcgg ggctttaccc cggtgattat ctcgtgcggg tcgggcagga gcttgccggt gagtttggca caaaacttct ggaaatgnct gaagccgaag cactggncat cgtgaangac cgcacgatcg acgccatgat ggcaatgatc cgcgctgatc tcgacgcgct gaacgtgcat catgatgtct tctattcgag cgcaagctac atgtcgacca tgcgcgcncg atccgtaacg cgatcaatga nctgacgctc nagggccatg ttannnngna anntgnnncc tccganggnn gttgccngan annnnnnnnn cgtgagcana ngctgtncgt nntacgnaan tngnnnnacn anatnacntc nctgatgaag tcganggnnc ctanttncng nngacgngnc ntanttcnng nnnnnnncnn nnnnnnnnnn nnnnncnngg nnnnnntcnn nnnnngnaan nnnnnnnnnn nnnntnncnn nnnnnnnnnn nnnnnnnnnn nnnnncnnnn nnnnnnnt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMNIFADFDA RIKKTFQDID LKPKDGGELD LSRIGVEPPR DASHGDIATN AAMVLSKAVG QNPRELAARI AEALKADEDV ESVDVAGPGF INLRLKASYW QRELLVMLNE GTDFGRSRLG AGKKVNVEYV SANPTGPMHV GHCRGAVVGD VLANLLKFAG YDVVKEYYIN DAGAQIDVLA RSVMLRYREA LGESIGEIPA GLYPGDYLVR VGQELAGEFG TKLLEMPEAE ALAIVKDRTI DAMMAMIRAD LDALNVHHDV FYSERKLHVD HARAIRNAIN DLTLKGHVYK GKLPPPKGRL PEDWEDREQT LFRSTEVGDD IDRPLMKSDG SFTYFAGDVT YFKDKYDRGF NEMIYVLGAD HGGYVKRLEA VARAVSDGKA KLTVLLCQLV KLFRNGEPVR MSKRAGEFIT LRDVVDEVGR DPVRFMMLYR KNDAPLDFDF AKVTEQSKDN PVFYVQYASA RCHSVFRQAA DQLGLVDLDR VAMGSHFEKL TDESEIALVR KLAEYPRLIE SAAIHQEPHR LAFYLYDLAS SFHSQWNRGA ENPDLRFIKV NDPDLSLARL GLVQVVSDVL TSGLTIIGAD APTEMR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatatcttt gcagatttcg atgcacgtat taaaaaaacg tttcaagata ttgatctgaa accaaaagat ggtggtgaac ttgacctttc acgcataggt gtcgagcccc cgcgcgatgc ttcgcatggc gatatcgcga ccaatgccgc aatggtgctt tccaaggcgg tcggccagaa cccgcgcgag cttgctgccc gcattgccga ggcgctgaag gccgatgagg atgtcgaatc cgtcgatgtc gccggtccgg gcttcatcaa tctgcgcctc aaggccagct attggcagcg cgagcttttg gtgatgctga acgagggaac ggattttggc cgctccaggc ttggcgcggg caagaaggtc aatgtcgaat atgtatcggc caaccctacg ggcccgatgc atgtcggcca ttgccggggc gcggttgtgg gtgatgtgct ggcgaacctg ctgaaattcg cgggctatga cgtcgtcaag gaatattaca tcaacgatgc gggcgcgcag atcgatgttc tcgcgcgttc tgtgatgctg cgctatcgtg aggcactggg cgaaagcatc ggcgagattc cggcggggct ttaccccggt gattatctcg tgcgggtcgg gcaggagctt gccggtgagt ttggcacaaa acttctggaa atgcctgaag ccgaagcact ggccatcgtg aaggaccgca cgatcgacgc catgatggca atgatccgcg ctgatctcga cgcgctgaac gtgcatcatg atgtcttcta ttccgagcgc aagctacatg tcgaccatgc gcgcgcgatc cgtaacgcga tcaatgatct gacgctcaag ggccatgttt ataagggcaa gctgccgcct ccgaaggggc ggttgccgga agattgggaa gaccgtgagc agacgctgtt ccgttctacg gaagtgggcg acgatattga ccgtccgctg atgaagtccg atggctcgtt cacctatttt gcgggtgacg tgacctattt caaggacaag tacgaccgcg gcttcaatga gatgatctat gtgctcggcg cggatcatgg cggttatgtc aagcgtctgg aagccgttgc gcgtgccgtt tccgatggca aggccaaatt gaccgtgctc ctgtgccagc tcgtgaagct gttccgcaat ggcgagccgg tgcgcatgtc caagcgggca ggcgagttca ttacgctgcg cgatgtggtg gacgaggtcg gtcgcgatcc ggtccgcttc atgatgcttt accggaagaa cgatgcgcca ttggacttcg acttcgccaa ggtgacggag cagtccaagg acaatccggt tttctatgtg caatatgctt ccgcgcgctg ccattcggtt ttccgtcagg ctgcggacca gcttgggctt gtcgatcttg accgcgtggc catggggtcg cattttgaaa agctgaccga cgagagcgaa attgcgctcg ttcgcaagct tgcggaatac ccgcgcctga tcgaatcggc agccattcat caggagccgc atcgcctggc tttctacctc tacgacctcg ccagttcgtt ccattcgcaa tggaacagag gcgcggaaaa tccggactta cgttttatta aggttaacga tccagacttg tcgctggcca ggctagggct ggtgcaggtt gtttccgatg tgctgacgtc aggattgacg attatcggcg cggatgctcc tacggagatg cgcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for BrabA.00164.a.A1.PW25627

PURIFICATION DATe:	9/29/2008
CONCENTRATION:	27.03mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	9
VIAL VOLUME:	100µl
PERCENT IDENTITY:	89
PERCENT COVERAGE:	37

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAATATCTTT GCAGATTTCG ATGCACGTAT TAAAAAAACG TTTCAAGATA TTGATCTGAA ACCAAAAGAT GGTGGTGAAC TTGACCTTTC ACGCATAGGT GTCGAGCCCC CGCGCGATGC TTCGCATGGC GATATCGCGA CCAATGCCGC AATGGTGCTT TCCAAGGCGG TCGGCCAGAA CCCGCGCGAG CTTGCTGCCC GCATTGCCGA GGCGCTGAAG GCCGATGAGG ATGTCGAATC CGTCGATGTC GCCGGTCCGG GCTTCATCAA TCTGCGCCTC AAGGCCAGCT ATTGGCAGCG CGAGCTTTTG GTGATGCTGA ACGAGGGAAC GGATTTTGGC CGCTCCAGGC TTGGCGCGGG CAAGAAGGTC AATGTCGAAT ATGTATCGGC CAACCCTACG GGCCCGATGC ATGTCGGCCA TTGCCGGGGC GCGGTTGTGG GTGATGTGCT GGCGAACCTG CTGAAATTCG CGGGCTATGA CGTCGTCAAG GAATATTACA TCAACGATGC GGGCGCGCAG ATCGATGTTC TCGCGCGTTC TGTGATGCTG CGCTATCGTG AGGCACTGGG CGAAAGCATC GGCGAGATTC CGGCGGGGCT TTACCCCGGT GATTATCTCG TGCGGGTCGG GCAGGAGCTT GCCGGTGAGT TTGGCACAAA ACTTCTGGAA ATGCCTGAAG CCGAAGCACT GGCCATCGTG AAGGACCGCA CGATCGACGC CATGATGGCA ATGATCCGCG CTGATCTCGA CGCGCTGAAC GTGCATCATG ATGTCTTCTA TTCCGAGCGC AAGCTACATG TCGACCATGC GCGCGCGATC CGTAACGCGA TCAATGATCT GACGCTCAAG GGCCATGTTT ATAAGGGCAA GCTGCCGCCT CCGAAGGGGC GGTTGCCGGA AGATTGGGAA GACCGTGAGC AGACGCTGTT CCGTTCTACG GAAGTGGGCG ACGATATTGA CCGTCCGCTG ATGAAGTCCG ATGGCTCGTT CACCTATTTT GCGGGTGACG TGACCTATTT CAAGGACAAG TACGACCGCG GCTTCAATGA GATGATCTAT GTGCTCGGCG CGGATCATGG CGGTTATGTC AAGCGTCTGG AAGCCGTTGC GCGTGCCGTT TCCGATGGCA AGGCCAAATT GACCGTGCTC CTGTGCCAGC TCGTGAAGCT GTTCCGCAAT GGCGAGCCGG TGCGCATGTC CAAGCGGGCA GGCGAGTTCA TTACGCTGCG CGATGTGGTG GACGAGGTCG GTCGCGATCC GGTCCGCTTC ATGATGCTTT ACCGGAAGAA CGATGCGCCA TTGGACTTCG ACTTCGCCAA GGTGACGGAG CAGTCCAAGG ACAATCCGGT TTTCTATGTG CAATATGCTT CCGCGCGCTG CCATTCGGTT TTCCGTCAGG CTGCGGACCA GCTTGGGCTT GTCGATCTTG ACCGCGTGGC CATGGGGTCG CATTTTGAAA AGCTGACCGA CGAGAGCGAA ATTGCGCTCG TTCGCAAGCT TGCGGAATAC CCGCGCCTGA TCGAATCGGC AGCCATTCAT CAGGAGCCGC ATCGCCTGGC TTTCTACCTC TACGACCTCG CCAGTTCGTT CCATTCGCAA TGGAACAGAG GCGCGGAAAA TCCGGACTTA CGTTTTATTA AGGTTAACGA TCCAGACTTG TCGCTGGCCA GGCTAGGGCT GGTGCAGGTT GTTTCCGATG TGCTGACGTC AGGATTGACG ATTATCGGCG CGGATGCTCC TACGGAGATG CGCAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA