BrabA.00163.a

phenylalanyl-tRNA synthetase subunit alpha

CENTER ID: BrabA.00163.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00163.a.A1.GE25581 Full length( BrabA.00163.a ) 1 369
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00163.a.A1.PW25626 Full length( BrabA.00163.a ) 1 369
BrabA.00163.a.A1.PW27586 Full length( BrabA.00163.a ) 1 369
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_127036
PATRIC ID: fig|359391.4.peg.2211
RefSeq: YP_415447.1
UniProt: Q2YQV5
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDLEQLERQ ILEDIAAAVD EQGIEAVRVA ALGKKGTVSE KLKTLGGMSP EERQMQGPAI NGLKNRVTEA LSERRTELRK AAVAARLERE KVDVTLPVRE SAASRGRIHP ISQVIDEITA IFADMGFSIA EGPDIETDYY NFTALNFPEG HPAREMHDTF FFNPDEKGER KLLRTHTSPV QVHTMEKFAA MRDKEGRDEP IRIVIPGKTY RMDSDATHSP MFHQVEGLVV DKSANVANMK WVLEEFCKAF FEVPSVKMRM RPSFFPFTEP SVEVDIQCDR SGPHVKFGEG NDWLEILGCG MVHPNVLRMS GYDPEVYQGF AWGMGIDRIA MLKYGMPDLR AFFDADVRWI EHYGFRPLDI PTLFGGLSA
NT Sequence
ATGAGCGATC TAGAACAACT CGAACGCCAG ATTCTCGAAG ACATTGCCGC GGCAGTGGAT GAGCAGGGTA TTGAGGCCGT GCGCGTGGCA GCGCTTGGCA AGAAAGGCAC GGTTTCGGAA AAGCTGAAGA CGCTGGGCGG CATGTCGCCG GAAGAGCGCC AGATGCAGGG GCCGGCCATC AATGGCCTGA AGAACCGCGT CACCGAGGCC TTGAGCGAGC GCAGGACCGA ACTGCGCAAG GCGGCGGTTG CCGCGCGTCT GGAGCGTGAA AAGGTTGATG TGACGCTCCC GGTGCGTGAA AGCGCGGCAT CGCGCGGGCG CATCCATCCT ATTTCGCAGG TGATCGACGA GATCACGGCG ATTTTCGCGG ATATGGGTTT TTCCATTGCC GAAGGTCCGG ATATCGAGAC CGACTATTAT AATTTCACCG CGCTCAACTT CCCCGAAGGC CATCCGGCGC GCGAGATGCA CGACACGTTC TTCTTCAACC CGGATGAAAA GGGCGAGCGC AAGTTGCTGC GCACGCACAC TTCGCCGGTG CAGGTGCATA CGATGGAGAA GTTCGCCGCC ATGCGCGATA AGGAAGGCCG CGACGAGCCG ATTCGCATCG TCATCCCCGG CAAGACCTAT CGTATGGATT CCGACGCCAC CCATTCGCCG ATGTTCCATC AGGTCGAGGG GCTGGTGGTC GACAAGTCGG CCAATGTCGC CAATATGAAG TGGGTGCTGG AAGAGTTCTG CAAAGCCTTC TTTGAAGTGC CTTCGGTGAA GATGCGTATG CGCCCGTCCT TCTTCCCGTT CACCGAGCCG TCTGTTGAAG TCGATATCCA GTGCGACCGC TCCGGCCCGC ATGTGAAATT CGGCGAGGGC AATGACTGGC TGGAAATTCT GGGCTGCGGC ATGGTGCATC CCAATGTCTT GCGCATGTCG GGCTATGACC CGGAAGTCTA TCAGGGCTTT GCCTGGGGCA TGGGCATCGA CCGCATTGCC ATGCTGAAAT ACGGAATGCC GGATCTGCGC GCCTTCTTCG ACGCCGATGT GCGCTGGATC GAGCATTACG GTTTCCGTCC GCTCGACATT CCGACGCTTT TCGGCGGATT GAGCGCG
Details for BrabA.00163.a.A1.GE25581
HARVESTED ON: 9/10/2008
SEQUENCED ON: 3/6/2009
EXPECTED MW: 43kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDLEQLER QILEDIAAAV DEQGIEAVRV AALGKKGTVS EKLKTLGGMS PEERQMQGPA INGLKNRVTE ALSERRTELR KAAVAARLER EKVDVTLPVR ESAASRGRIH PISQVIDEIT AIFADMGFSI AEGPDIETDY YNFTALNFPE GHPAREMHDT FFFNPDEKGE RKLLRTHTSP VQVHTMEKFA AMRDKEGRDE PIRIVIPGKT YRMDSDATHS PMFHQVEGLV VDKSANVANM MWVLEEFCKA FFEVPSVKMR MRPSFFPFTE PSVEVDIQCD RSGPHVKFGE GNDWLEILGC GMVHPNVLRM SGYDPEVYQG FAWGMGIDRI AMLKYGVPDL RAFFDADVRW IEHYGFRPLD IPTLFGGLSA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagcg atctagaaca actcgaacgc cagattctcg aagacattgc cgcggcagtg gatgagcagg gtattgaggc cgtgcgcgtg gcagcgcttg gcaagaaagg cacggtttcg gaaaagctga agacgctggg cggcatgtcg ccggaagagc gccagatgca ggggccggcc atcaatggcc tgaagaaccg cgtcaccgag gccttgagcg agcgcaggac cgaactgcgc aaggcggcgg ttgccgcgcg tctggagcgt gaaaaggttg atgtgacgct cccggtgcgt gaaagcgcgg catcgcgcgg gcgcatccat cctatttcgc aggtgatcga cgagatcacg gcgattttcg cggatatggg tttttccatt gccgaaggtc cggatatcga gaccgactat tataatttca ccgcgctcaa cttccccgaa ggccatccgg cgcgcgagat gcacgacacg ttcttcttca acccggatga aaagggcgag cgcaagttgc tgcgcacgca cacttcgccg gtgcaggtgc atacgatgga gaagttcgcc gccatgcgcg ataaggaagg ccgcgacgag ccgattcgca tcgtcatccc cggcaagacc tatcgtatgg attccgacgc cacccattcg ccgatgttcc atcaggtcga ggggctggtg gtcgacaagt cggccaatgt cgccaatatg atgtgggtgc tggaagagtt ctgcaaagcc ttctttgaag tgccttcggt gaagatgcgt atgcgcccgt ccttcttccc gttcaccgag ccgtctgttg aagtcgatat ccagtgcgac cgctccggcc cgcatgtgaa attcggcgag ggcaatgact ggctggaaat tctgggctgc ggcatggtgc atcccaatgt cttgcgcatg tcgggctatg acccggaagt ctatcagggc tttgcctggg gcatgggcat cgaccgcatt gccatgctga aatacggagt gccggatctg cgcgccttct tcgacgccga tgtgcgctgg atcgagcatt acggtttccg tccgctcgac attccgacgc ttttcggcgg attgagcgcg taataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDLEQLER QILEDIAAAV DEQGIEAVRV AALGKKGTVS EKLKTLGGMS PEERQMQGPA INGLKNRVTE ALSERRTELR KAAVAARLER EKVDVTLPVR ESAASRGRIH PISQVIDEIT AIFADMGFSI AEGPDIETDY YNFTALNFPE GHPAREMHDT FFFNPDEKGE RKLLRTHTSP VQVHTMEKFA AMRDKEGRDE PIRIVIPGKT YRMDSDATHS PMFHQVEGLV VDKSANVANM KWVLEEFCKA FFEVPSVKMR MRPSFFPFTE PSVEVDIQCD RSGPHVKFGE GNDWLEILGC GMVHPNVLRM SGYDPEVYQG FAWGMGIDRI AMLKYGMPDL RAFFDADVRW IEHYGFRPLD IPTLFGGLSA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgatcta gaacaactcg aacgccagat tctcgaagac attgccgcgg cagtggatga gcagggtatt gaggccgtgc gcgtggcagc gcttggcaag aaaggcacgg tttcggaaaa gctgaagacg ctgggcggca tgtcgccgga agagcgccag atgcaggggc cggccatcaa tggcctgaag aaccgcgtca ccgaggcctt gagcgagcgc aggaccgaac tgcgcaaggc ggcggttgcc gcgcgtctgg agcgtgaaaa ggttgatgtg acgctcccgg tgcgtgaaag cgcggcatcg cgcgggcgca tccatcctat ttcgcaggtg atcgacgaga tcacggcgat tttcgcggat atgggttttt ccattgccga aggtccggat atcgagaccg actattataa tttcaccgcg ctcaacttcc ccgaaggcca tccggcgcgc gagatgcacg acacgttctt cttcaacccg gatgaaaagg gcgagcgcaa gttgctgcgc acgcacactt cgccggtgca ggtgcatacg atggagaagt tcgccgccat gcgcgataag gaaggccgcg acgagccgat tcgcatcgtc atccccggca agacctatcg tatggattcc gacgccaccc attcgccgat gttccatcag gtcgaggggc tggtggtcga caagtcggcc aatgtcgcca atatgaagtg ggtgctggaa gagttctgca aagccttctt tgaagtgcct tcggtgaaga tgcgtatgcg cccgtccttc ttcccgttca ccgagccgtc tgttgaagtc gatatccagt gcgaccgctc cggcccgcat gtgaaattcg gcgagggcaa tgactggctg gaaattctgg gctgcggcat ggtgcatccc aatgtcttgc gcatgtcggg ctatgacccg gaagtctatc agggctttgc ctggggcatg ggcatcgacc gcattgccat gctgaaatac ggaatgccgg atctgcgcgc cttcttcgac gccgatgtgc gctggatcga gcattacggt ttccgtccgc tcgacattcc gacgcttttc ggcggattga gcgcgaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BrabA.00163.a.A1.PW25626
PURIFICATION DATe: 9/29/2008
CONCENTRATION: 9.17mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 100µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDLEQLER QILEDIAAAV DEQGIEAVRV AALGKKGTVS EKLKTLGGMS PEERQMQGPA INGLKNRVTE ALSERRTELR KAAVAARLER EKVDVTLPVR ESAASRGRIH PISQVIDEIT AIFADMGFSI AEGPDIETDY YNFTALNFPE GHPAREMHDT FFFNPDEKGE RKLLRTHTSP VQVHTMEKFA AMRDKEGRDE PIRIVIPGKT YRMDSDATHS PMFHQVEGLV VDKSANVANM MWVLEEFCKA FFEVPSVKMR MRPSFFPFTE PSVEVDIQCD RSGPHVKFGE GNDWLEILGC GMVHPNVLRM SGYDPEVYQG FAWGMGIDRI AMLKYGVPDL RAFFDADVRW IEHYGFRPLD IPTLFGGLSA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagcg atctagaaca actcgaacgc cagattctcg aagacattgc cgcggcagtg gatgagcagg gtattgaggc cgtgcgcgtg gcagcgcttg gcaagaaagg cacggtttcg gaaaagctga agacgctggg cggcatgtcg ccggaagagc gccagatgca ggggccggcc atcaatggcc tgaagaaccg cgtcaccgag gccttgagcg agcgcaggac cgaactgcgc aaggcggcgg ttgccgcgcg tctggagcgt gaaaaggttg atgtgacgct cccggtgcgt gaaagcgcgg catcgcgcgg gcgcatccat cctatttcgc aggtgatcga cgagatcacg gcgattttcg cggatatggg tttttccatt gccgaaggtc cggatatcga gaccgactat tataatttca ccgcgctcaa cttccccgaa ggccatccgg cgcgcgagat gcacgacacg ttcttcttca acccggatga aaagggcgag cgcaagttgc tgcgcacgca cacttcgccg gtgcaggtgc atacgatgga gaagttcgcc gccatgcgcg ataaggaagg ccgcgacgag ccgattcgca tcgtcatccc cggcaagacc tatcgtatgg attccgacgc cacccattcg ccgatgttcc atcaggtcga ggggctggtg gtcgacaagt cggccaatgt cgccaatatg atgtgggtgc tggaagagtt ctgcaaagcc ttctttgaag tgccttcggt gaagatgcgt atgcgcccgt ccttcttccc gttcaccgag ccgtctgttg aagtcgatat ccagtgcgac cgctccggcc cgcatgtgaa attcggcgag ggcaatgact ggctggaaat tctgggctgc ggcatggtgc atcccaatgt cttgcgcatg tcgggctatg acccggaagt ctatcagggc tttgcctggg gcatgggcat cgaccgcatt gccatgctga aatacggagt gccggatctg cgcgccttct tcgacgccga tgtgcgctgg atcgagcatt acggtttccg tccgctcgac attccgacgc ttttcggcgg attgagcgcg taataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDLEQLER QILEDIAAAV DEQGIEAVRV AALGKKGTVS EKLKTLGGMS PEERQMQGPA INGLKNRVTE ALSERRTELR KAAVAARLER EKVDVTLPVR ESAASRGRIH PISQVIDEIT AIFADMGFSI AEGPDIETDY YNFTALNFPE GHPAREMHDT FFFNPDEKGE RKLLRTHTSP VQVHTMEKFA AMRDKEGRDE PIRIVIPGKT YRMDSDATHS PMFHQVEGLV VDKSANVANM KWVLEEFCKA FFEVPSVKMR MRPSFFPFTE PSVEVDIQCD RSGPHVKFGE GNDWLEILGC GMVHPNVLRM SGYDPEVYQG FAWGMGIDRI AMLKYGMPDL RAFFDADVRW IEHYGFRPLD IPTLFGGLSA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGATCTA GAACAACTCG AACGCCAGAT TCTCGAAGAC ATTGCCGCGG CAGTGGATGA GCAGGGTATT GAGGCCGTGC GCGTGGCAGC GCTTGGCAAG AAAGGCACGG TTTCGGAAAA GCTGAAGACG CTGGGCGGCA TGTCGCCGGA AGAGCGCCAG ATGCAGGGGC CGGCCATCAA TGGCCTGAAG AACCGCGTCA CCGAGGCCTT GAGCGAGCGC AGGACCGAAC TGCGCAAGGC GGCGGTTGCC GCGCGTCTGG AGCGTGAAAA GGTTGATGTG ACGCTCCCGG TGCGTGAAAG CGCGGCATCG CGCGGGCGCA TCCATCCTAT TTCGCAGGTG ATCGACGAGA TCACGGCGAT TTTCGCGGAT ATGGGTTTTT CCATTGCCGA AGGTCCGGAT ATCGAGACCG ACTATTATAA TTTCACCGCG CTCAACTTCC CCGAAGGCCA TCCGGCGCGC GAGATGCACG ACACGTTCTT CTTCAACCCG GATGAAAAGG GCGAGCGCAA GTTGCTGCGC ACGCACACTT CGCCGGTGCA GGTGCATACG ATGGAGAAGT TCGCCGCCAT GCGCGATAAG GAAGGCCGCG ACGAGCCGAT TCGCATCGTC ATCCCCGGCA AGACCTATCG TATGGATTCC GACGCCACCC ATTCGCCGAT GTTCCATCAG GTCGAGGGGC TGGTGGTCGA CAAGTCGGCC AATGTCGCCA ATATGAAGTG GGTGCTGGAA GAGTTCTGCA AAGCCTTCTT TGAAGTGCCT TCGGTGAAGA TGCGTATGCG CCCGTCCTTC TTCCCGTTCA CCGAGCCGTC TGTTGAAGTC GATATCCAGT GCGACCGCTC CGGCCCGCAT GTGAAATTCG GCGAGGGCAA TGACTGGCTG GAAATTCTGG GCTGCGGCAT GGTGCATCCC AATGTCTTGC GCATGTCGGG CTATGACCCG GAAGTCTATC AGGGCTTTGC CTGGGGCATG GGCATCGACC GCATTGCCAT GCTGAAATAC GGAATGCCGG ATCTGCGCGC CTTCTTCGAC GCCGATGTGC GCTGGATCGA GCATTACGGT TTCCGTCCGC TCGACATTCC GACGCTTTTC GGCGGATTGA GCGCGAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA
Details for BrabA.00163.a.A1.PW27586
PURIFICATION DATe: 9/22/2009
CONCENTRATION: 20mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDLEQLER QILEDIAAAV DEQGIEAVRV AALGKKGTVS EKLKTLGGMS PEERQMQGPA INGLKNRVTE ALSERRTELR KAAVAARLER EKVDVTLPVR ESAASRGRIH PISQVIDEIT AIFADMGFSI AEGPDIETDY YNFTALNFPE GHPAREMHDT FFFNPDEKGE RKLLRTHTSP VQVHTMEKFA AMRDKEGRDE PIRIVIPGKT YRMDSDATHS PMFHQVEGLV VDKSANVANM MWVLEEFCKA FFEVPSVKMR MRPSFFPFTE PSVEVDIQCD RSGPHVKFGE GNDWLEILGC GMVHPNVLRM SGYDPEVYQG FAWGMGIDRI AMLKYGVPDL RAFFDADVRW IEHYGFRPLD IPTLFGGLSA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagcg atctagaaca actcgaacgc cagattctcg aagacattgc cgcggcagtg gatgagcagg gtattgaggc cgtgcgcgtg gcagcgcttg gcaagaaagg cacggtttcg gaaaagctga agacgctggg cggcatgtcg ccggaagagc gccagatgca ggggccggcc atcaatggcc tgaagaaccg cgtcaccgag gccttgagcg agcgcaggac cgaactgcgc aaggcggcgg ttgccgcgcg tctggagcgt gaaaaggttg atgtgacgct cccggtgcgt gaaagcgcgg catcgcgcgg gcgcatccat cctatttcgc aggtgatcga cgagatcacg gcgattttcg cggatatggg tttttccatt gccgaaggtc cggatatcga gaccgactat tataatttca ccgcgctcaa cttccccgaa ggccatccgg cgcgcgagat gcacgacacg ttcttcttca acccggatga aaagggcgag cgcaagttgc tgcgcacgca cacttcgccg gtgcaggtgc atacgatgga gaagttcgcc gccatgcgcg ataaggaagg ccgcgacgag ccgattcgca tcgtcatccc cggcaagacc tatcgtatgg attccgacgc cacccattcg ccgatgttcc atcaggtcga ggggctggtg gtcgacaagt cggccaatgt cgccaatatg atgtgggtgc tggaagagtt ctgcaaagcc ttctttgaag tgccttcggt gaagatgcgt atgcgcccgt ccttcttccc gttcaccgag ccgtctgttg aagtcgatat ccagtgcgac cgctccggcc cgcatgtgaa attcggcgag ggcaatgact ggctggaaat tctgggctgc ggcatggtgc atcccaatgt cttgcgcatg tcgggctatg acccggaagt ctatcagggc tttgcctggg gcatgggcat cgaccgcatt gccatgctga aatacggagt gccggatctg cgcgccttct tcgacgccga tgtgcgctgg atcgagcatt acggtttccg tccgctcgac attccgacgc ttttcggcgg attgagcgcg taataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDLEQLER QILEDIAAAV DEQGIEAVRV AALGKKGTVS EKLKTLGGMS PEERQMQGPA INGLKNRVTE ALSERRTELR KAAVAARLER EKVDVTLPVR ESAASRGRIH PISQVIDEIT AIFADMGFSI AEGPDIETDY YNFTALNFPE GHPAREMHDT FFFNPDEKGE RKLLRTHTSP VQVHTMEKFA AMRDKEGRDE PIRIVIPGKT YRMDSDATHS PMFHQVEGLV VDKSANVANM KWVLEEFCKA FFEVPSVKMR MRPSFFPFTE PSVEVDIQCD RSGPHVKFGE GNDWLEILGC GMVHPNVLRM SGYDPEVYQG FAWGMGIDRI AMLKYGMPDL RAFFDADVRW IEHYGFRPLD IPTLFGGLSA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGATCTA GAACAACTCG AACGCCAGAT TCTCGAAGAC ATTGCCGCGG CAGTGGATGA GCAGGGTATT GAGGCCGTGC GCGTGGCAGC GCTTGGCAAG AAAGGCACGG TTTCGGAAAA GCTGAAGACG CTGGGCGGCA TGTCGCCGGA AGAGCGCCAG ATGCAGGGGC CGGCCATCAA TGGCCTGAAG AACCGCGTCA CCGAGGCCTT GAGCGAGCGC AGGACCGAAC TGCGCAAGGC GGCGGTTGCC GCGCGTCTGG AGCGTGAAAA GGTTGATGTG ACGCTCCCGG TGCGTGAAAG CGCGGCATCG CGCGGGCGCA TCCATCCTAT TTCGCAGGTG ATCGACGAGA TCACGGCGAT TTTCGCGGAT ATGGGTTTTT CCATTGCCGA AGGTCCGGAT ATCGAGACCG ACTATTATAA TTTCACCGCG CTCAACTTCC CCGAAGGCCA TCCGGCGCGC GAGATGCACG ACACGTTCTT CTTCAACCCG GATGAAAAGG GCGAGCGCAA GTTGCTGCGC ACGCACACTT CGCCGGTGCA GGTGCATACG ATGGAGAAGT TCGCCGCCAT GCGCGATAAG GAAGGCCGCG ACGAGCCGAT TCGCATCGTC ATCCCCGGCA AGACCTATCG TATGGATTCC GACGCCACCC ATTCGCCGAT GTTCCATCAG GTCGAGGGGC TGGTGGTCGA CAAGTCGGCC AATGTCGCCA ATATGAAGTG GGTGCTGGAA GAGTTCTGCA AAGCCTTCTT TGAAGTGCCT TCGGTGAAGA TGCGTATGCG CCCGTCCTTC TTCCCGTTCA CCGAGCCGTC TGTTGAAGTC GATATCCAGT GCGACCGCTC CGGCCCGCAT GTGAAATTCG GCGAGGGCAA TGACTGGCTG GAAATTCTGG GCTGCGGCAT GGTGCATCCC AATGTCTTGC GCATGTCGGG CTATGACCCG GAAGTCTATC AGGGCTTTGC CTGGGGCATG GGCATCGACC GCATTGCCAT GCTGAAATAC GGAATGCCGG ATCTGCGCGC CTTCTTCGAC GCCGATGTGC GCTGGATCGA GCATTACGGT TTCCGTCCGC TCGACATTCC GACGCTTTTC GGCGGATTGA GCGCGAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA