BrabA.00156.a

threonyl-tRNA synthetase

CENTER ID: BrabA.00156.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00156.a.A1.GE25578 Full length( BrabA.00156.a ) 1 658
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00156.a.A1.PW25623 Full length( BrabA.00156.a ) 1 658
BrabA.00156.a.A1.PW27576 Full length( BrabA.00156.a ) 1 658
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_126764
PATRIC ID: fig|359391.4.peg.1138
RefSeq: YP_414489.1
UniProt: Q2YQ07
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSNTVSLQFP DGSVREYDAS MTGAALAESI SKSLAKKAVA YAVDGTVRDL SDPLGASGKL EIITREDPRA LELIRHDTAH VLAEAVQELF PGTQVTIGPV IENGFYYDFA RNEPFTLDDL PVIEKKMREI IQRNKPFTKE VWSREKAKQV FSDKGESYKV ELVDAIPAGQ DLKIYYQGDW FDLCRGPHMA STGQIGNSFK LMKVAGAYWR GDANNPMLTR IYGTAFANDN DLQAYLHMLE EAEKRDHRRL GREMDLFHFQ EEGPGVVFWH AKGWKMFQNL VSYMRRRLDS HGYQEVNTPQ VLDKSLWETS GHWGWYRDNM FKVTVAGDDT DDDRVFALKP MNCPGHVQIF KHGLKSYRDL PIKLAEFGNV HRYEPSGALH GLMRVRGFTQ DDAHIFCTEE QMAAECLHIN DLILSVYKDF GFEEITIKLS TRPEKRVGSD ELWDRAESVM MTVLEQIRQQ SNNIKTGILP GEGAFYGPKF EYTLKDAIGR EWQCGTTQVD FNLPERFGAF YIGADSEKKQ PVMIHRAICG SMERFLGILI ENFAGHMPLW FAPVQVVVAT ITSDADEYAK EAAAKLKAAG LQVVTDLRNE KINYKVREHS LQKVPVILVC GKREAEEKTV NMRRLGSRDQ ESMTLDEAIA RLCEEATPPD LLRLKNAG
NT Sequence
ATGTCGAATA CCGTTTCCCT GCAATTTCCC GATGGTTCCG TACGTGAATA CGATGCATCG ATGACAGGGG CCGCCCTTGC GGAATCAATT TCCAAGTCGC TTGCCAAGAA GGCTGTCGCT TACGCTGTTG ATGGCACGGT TCGTGATCTT TCCGATCCGC TTGGCGCTTC CGGCAAGCTG GAAATCATCA CCCGCGAAGA CCCGCGCGCG CTGGAACTCA TCCGCCACGA CACTGCCCAT GTGCTTGCAG AAGCCGTGCA GGAGCTTTTT CCCGGAACGC AGGTAACGAT CGGCCCCGTG ATCGAAAACG GCTTCTATTA CGACTTTGCG CGCAACGAGC CGTTTACGCT CGATGACCTG CCGGTGATCG AAAAGAAGAT GCGCGAGATC ATTCAGCGCA ACAAGCCGTT CACCAAGGAA GTCTGGTCAC GCGAGAAGGC CAAACAGGTT TTTTCCGACA AGGGCGAAAG CTACAAGGTC GAGCTGGTCG ATGCCATTCC GGCCGGGCAG GACCTCAAGA TCTATTATCA GGGTGACTGG TTCGACCTTT GCCGCGGGCC GCATATGGCT TCCACGGGTC AGATCGGCAA TTCATTCAAG TTGATGAAGG TGGCGGGCGC TTATTGGCGC GGCGATGCCA ATAATCCGAT GCTGACCCGC ATCTACGGCA CCGCCTTTGC AAACGATAAT GACCTTCAAG CCTATCTCCA TATGCTTGAG GAAGCGGAGA AGCGCGACCA CCGCCGCCTT GGCCGCGAAA TGGACCTGTT CCATTTCCAG GAAGAGGGGC CGGGCGTCGT GTTCTGGCAC GCCAAAGGCT GGAAGATGTT CCAGAATCTG GTGAGCTATA TGCGCCGCCG CCTCGACAGC CATGGCTATC AGGAAGTGAA TACGCCGCAG GTGCTGGACA AGTCGCTGTG GGAAACCTCC GGCCACTGGG GCTGGTATCG CGACAATATG TTCAAGGTTA CGGTTGCCGG TGACGATACC GACGATGACC GTGTCTTTGC GCTGAAGCCG ATGAACTGCC CCGGCCATGT GCAGATATTC AAGCATGGCC TTAAATCATA CCGCGATCTT CCTATAAAGC TTGCGGAATT CGGCAATGTG CACCGCTATG AGCCTTCCGG TGCGCTGCAC GGGCTGATGC GTGTGCGCGG TTTCACGCAG GACGATGCGC ATATTTTCTG CACCGAAGAG CAGATGGCGG CGGAATGCCT GCACATCAAT GATCTTATCT TGTCGGTCTA TAAGGATTTC GGCTTCGAGG AAATCACCAT CAAGCTCTCG ACGCGCCCGG AAAAGCGCGT TGGCTCGGAT GAGCTTTGGG ATCGTGCCGA AAGCGTGATG ATGACCGTTC TGGAGCAGAT CCGGCAGCAG TCGAACAATA TCAAGACCGG CATTCTGCCG GGCGAGGGTG CGTTCTACGG GCCGAAGTTT GAATATACGC TGAAGGACGC CATCGGCCGT GAATGGCAGT GCGGCACCAC GCAGGTCGAT TTCAACCTGC CGGAACGCTT CGGCGCCTTC TATATCGGCG CGGATTCGGA AAAGAAGCAG CCGGTCATGA TCCATCGCGC CATCTGCGGC TCGATGGAAC GTTTCCTCGG CATTCTGATC GAGAATTTTG CCGGTCACAT GCCGCTCTGG TTTGCGCCGG TACAGGTGGT TGTTGCAACC ATCACGTCGG ATGCCGATGA ATATGCAAAG GAAGCGGCTG CGAAGCTCAA GGCTGCCGGT CTTCAGGTCG TGACCGATCT GCGCAACGAG AAGATCAACT ACAAGGTTCG CGAACATTCG CTCCAGAAAG TTCCGGTCAT CCTCGTTTGC GGCAAGCGTG AAGCAGAAGA AAAGACTGTC AACATGCGCC GTCTCGGCTC ACGCGATCAG GAATCCATGA CGCTGGATGA AGCGATTGCC CGGCTTTGCG AAGAGGCGAC CCCGCCCGAT CTGCTTCGCC TGAAAAATGC AGGC
Details for BrabA.00156.a.A1.GE25578
HARVESTED ON: 9/10/2008
SEQUENCED ON: 3/16/2012
EXPECTED MW: 76kDa
OBSERVED MW: 76kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 39
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSNTVSLQF PDGSVREYDA SMTGAALAES ISKSLAKKAV AYAVDGTVRD LSDPLGASGK LEIITREDPR ALELIRHDTA HVLAEAVQEL FPGTQVTIGP VIENGFYYDF ARNEPFTLDD LPVIEKKMRE IIQRNKPFTK EVWSREKAKQ VFSDKGESYK VELVDAIPAG QDLKIYYQGD WFDLCRGPHM ASTGQIGNSF KLMKVAGAYW RGDANNPMLT RIYGTAFAND NDLQAYLHML EEAEXRDHRR
Validated NT Sequence
tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtcgaa taccgtttcc ctgcaatttc ccgatggttc cgtacgtgaa tacgatgcat cgatgacagg ggccgccctt gcggaatcaa tttccaagtc gcttgccaag aaggctgtcg cttacgctgt tgatggcacg gttcgtgatc tttccgatcc gcttggcgct tccggcaagc tggaaatcat cacccgcgaa gacccgcgcg cgctggaact catccgccac gacactgccc atgtgcttgc agaagccgtg caggagcttt ttcccggaac gcaggtaacg atcggccccg tgatcgaaaa cggcttctat tacgactttg cgcgcaacga gccgtttacg ctcgatgacc tgccggtgat cgaaaagaag atgcgcgaga tcattcagcg caacaagccg ttcaccaagg aagtctggtc acgcgagaag gccaaacagg ttttttccga caagggcgaa agctacaagg tcgagctggt cgatgccatt ccggccgggc aggacctcaa gatctattat cagggtgact ggttcgacct ttgccgcggg ccgcatatgg cttccacggg tcagatcggc aattcattca agttgatgaa ggtggcgggc gcttattggc gcggcgatgc caataatccg atgctgaccc gcatctacgg caccgccttt gcaaacgata atgaccttca agcctatctc catatgcttg aggaagcgga gaancgcgac caccgccgcc tt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSNTVSLQF PDGSVREYDA SMTGAALAES ISKSLAKKAV AYAVDGTVRD LSDPLGASGK LEIITREDPR ALELIRHDTA HVLAEAVQEL FPGTQVTIGP VIENGFYYDF ARNEPFTLDD LPVIEKKMRE IIQRNKPFTK EVWSREKAKQ VFSDKGESYK VELVDAIPAG QDLKIYYQGD WFDLCRGPHM ASTGQIGNSF KLMKVAGAYW RGDANNPMLT RIYGTAFAND NDLQAYLHML EEAEKRDHRR LGREMDLFHF QEEGPGVVFW HAKGWKMFQN LVSYMRRRLD SHGYQEVNTP QVLDKSLWET SGHWGWYRDN MFKVTVAGDD TDDDRVFALK PMNCPGHVQI FKHGLKSYRD LPIKLAEFGN VHRYEPSGAL HGLMRVRGFT QDDAHIFCTE EQMAAECLHI NDLILSVYKD FGFEEITIKL STRPEKRVGS DELWDRAESV MMTVLEQIRQ QSNNIKTGIL PGEGAFYGPK FEYTLKDAIG REWQCGTTQV DFNLPERFGA FYIGADSEKK QPVMIHRAIC GSMERFLGIL IENFAGHMPL WFAPVQVVVA TITSDADEYA KEAAAKLKAA GLQVVTDLRN EKINYKVREH SLQKVPVILV CGKREAEEKT VNMRRLGSRD QESMTLDEAI ARLCEEATPP DLLRLKNAG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgaatacc gtttccctgc aatttcccga tggttccgta cgtgaatacg atgcatcgat gacaggggcc gcccttgcgg aatcaatttc caagtcgctt gccaagaagg ctgtcgctta cgctgttgat ggcacggttc gtgatctttc cgatccgctt ggcgcttccg gcaagctgga aatcatcacc cgcgaagacc cgcgcgcgct ggaactcatc cgccacgaca ctgcccatgt gcttgcagaa gccgtgcagg agctttttcc cggaacgcag gtaacgatcg gccccgtgat cgaaaacggc ttctattacg actttgcgcg caacgagccg tttacgctcg atgacctgcc ggtgatcgaa aagaagatgc gcgagatcat tcagcgcaac aagccgttca ccaaggaagt ctggtcacgc gagaaggcca aacaggtttt ttccgacaag ggcgaaagct acaaggtcga gctggtcgat gccattccgg ccgggcagga cctcaagatc tattatcagg gtgactggtt cgacctttgc cgcgggccgc atatggcttc cacgggtcag atcggcaatt cattcaagtt gatgaaggtg gcgggcgctt attggcgcgg cgatgccaat aatccgatgc tgacccgcat ctacggcacc gcctttgcaa acgataatga ccttcaagcc tatctccata tgcttgagga agcggagaag cgcgaccacc gccgccttgg ccgcgaaatg gacctgttcc atttccagga agaggggccg ggcgtcgtgt tctggcacgc caaaggctgg aagatgttcc agaatctggt gagctatatg cgccgccgcc tcgacagcca tggctatcag gaagtgaata cgccgcaggt gctggacaag tcgctgtggg aaacctccgg ccactggggc tggtatcgcg acaatatgtt caaggttacg gttgccggtg acgataccga cgatgaccgt gtctttgcgc tgaagccgat gaactgcccc ggccatgtgc agatattcaa gcatggcctt aaatcatacc gcgatcttcc tataaagctt gcggaattcg gcaatgtgca ccgctatgag ccttccggtg cgctgcacgg gctgatgcgt gtgcgcggtt tcacgcagga cgatgcgcat attttctgca ccgaagagca gatggcggcg gaatgcctgc acatcaatga tcttatcttg tcggtctata aggatttcgg cttcgaggaa atcaccatca agctctcgac gcgcccggaa aagcgcgttg gctcggatga gctttgggat cgtgccgaaa gcgtgatgat gaccgttctg gagcagatcc ggcagcagtc gaacaatatc aagaccggca ttctgccggg cgagggtgcg ttctacgggc cgaagtttga atatacgctg aaggacgcca tcggccgtga atggcagtgc ggcaccacgc aggtcgattt caacctgccg gaacgcttcg gcgccttcta tatcggcgcg gattcggaaa agaagcagcc ggtcatgatc catcgcgcca tctgcggctc gatggaacgt ttcctcggca ttctgatcga gaattttgcc ggtcacatgc cgctctggtt tgcgccggta caggtggttg ttgcaaccat cacgtcggat gccgatgaat atgcaaagga agcggctgcg aagctcaagg ctgccggtct tcaggtcgtg accgatctgc gcaacgagaa gatcaactac aaggttcgcg aacattcgct ccagaaagtt ccggtcatcc tcgtttgcgg caagcgtgaa gcagaagaaa agactgtcaa catgcgccgt ctcggctcac gcgatcagga atccatgacg ctggatgaag cgattgcccg gctttgcgaa gaggcgaccc cgcccgatct gcttcgcctg aaaaatgcag gcaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for BrabA.00156.a.A1.PW25623
PURIFICATION DATe: 9/29/2008
CONCENTRATION: 34.06mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 100µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 39
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSNTVSLQF PDGSVREYDA SMTGAALAES ISKSLAKKAV AYAVDGTVRD LSDPLGASGK LEIITREDPR ALELIRHDTA HVLAEAVQEL FPGTQVTIGP VIENGFYYDF ARNEPFTLDD LPVIEKKMRE IIQRNKPFTK EVWSREKAKQ VFSDKGESYK VELVDAIPAG QDLKIYYQGD WFDLCRGPHM ASTGQIGNSF KLMKVAGAYW RGDANNPMLT RIYGTAFAND NDLQAYLHML EEAEXRDHRR
Validated NT Sequence
tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtcgaa taccgtttcc ctgcaatttc ccgatggttc cgtacgtgaa tacgatgcat cgatgacagg ggccgccctt gcggaatcaa tttccaagtc gcttgccaag aaggctgtcg cttacgctgt tgatggcacg gttcgtgatc tttccgatcc gcttggcgct tccggcaagc tggaaatcat cacccgcgaa gacccgcgcg cgctggaact catccgccac gacactgccc atgtgcttgc agaagccgtg caggagcttt ttcccggaac gcaggtaacg atcggccccg tgatcgaaaa cggcttctat tacgactttg cgcgcaacga gccgtttacg ctcgatgacc tgccggtgat cgaaaagaag atgcgcgaga tcattcagcg caacaagccg ttcaccaagg aagtctggtc acgcgagaag gccaaacagg ttttttccga caagggcgaa agctacaagg tcgagctggt cgatgccatt ccggccgggc aggacctcaa gatctattat cagggtgact ggttcgacct ttgccgcggg ccgcatatgg cttccacggg tcagatcggc aattcattca agttgatgaa ggtggcgggc gcttattggc gcggcgatgc caataatccg atgctgaccc gcatctacgg caccgccttt gcaaacgata atgaccttca agcctatctc catatgcttg aggaagcgga gaancgcgac caccgccgcc tt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSNTVSLQF PDGSVREYDA SMTGAALAES ISKSLAKKAV AYAVDGTVRD LSDPLGASGK LEIITREDPR ALELIRHDTA HVLAEAVQEL FPGTQVTIGP VIENGFYYDF ARNEPFTLDD LPVIEKKMRE IIQRNKPFTK EVWSREKAKQ VFSDKGESYK VELVDAIPAG QDLKIYYQGD WFDLCRGPHM ASTGQIGNSF KLMKVAGAYW RGDANNPMLT RIYGTAFAND NDLQAYLHML EEAEKRDHRR LGREMDLFHF QEEGPGVVFW HAKGWKMFQN LVSYMRRRLD SHGYQEVNTP QVLDKSLWET SGHWGWYRDN MFKVTVAGDD TDDDRVFALK PMNCPGHVQI FKHGLKSYRD LPIKLAEFGN VHRYEPSGAL HGLMRVRGFT QDDAHIFCTE EQMAAECLHI NDLILSVYKD FGFEEITIKL STRPEKRVGS DELWDRAESV MMTVLEQIRQ QSNNIKTGIL PGEGAFYGPK FEYTLKDAIG REWQCGTTQV DFNLPERFGA FYIGADSEKK QPVMIHRAIC GSMERFLGIL IENFAGHMPL WFAPVQVVVA TITSDADEYA KEAAAKLKAA GLQVVTDLRN EKINYKVREH SLQKVPVILV CGKREAEEKT VNMRRLGSRD QESMTLDEAI ARLCEEATPP DLLRLKNAG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCGAATACC GTTTCCCTGC AATTTCCCGA TGGTTCCGTA CGTGAATACG ATGCATCGAT GACAGGGGCC GCCCTTGCGG AATCAATTTC CAAGTCGCTT GCCAAGAAGG CTGTCGCTTA CGCTGTTGAT GGCACGGTTC GTGATCTTTC CGATCCGCTT GGCGCTTCCG GCAAGCTGGA AATCATCACC CGCGAAGACC CGCGCGCGCT GGAACTCATC CGCCACGACA CTGCCCATGT GCTTGCAGAA GCCGTGCAGG AGCTTTTTCC CGGAACGCAG GTAACGATCG GCCCCGTGAT CGAAAACGGC TTCTATTACG ACTTTGCGCG CAACGAGCCG TTTACGCTCG ATGACCTGCC GGTGATCGAA AAGAAGATGC GCGAGATCAT TCAGCGCAAC AAGCCGTTCA CCAAGGAAGT CTGGTCACGC GAGAAGGCCA AACAGGTTTT TTCCGACAAG GGCGAAAGCT ACAAGGTCGA GCTGGTCGAT GCCATTCCGG CCGGGCAGGA CCTCAAGATC TATTATCAGG GTGACTGGTT CGACCTTTGC CGCGGGCCGC ATATGGCTTC CACGGGTCAG ATCGGCAATT CATTCAAGTT GATGAAGGTG GCGGGCGCTT ATTGGCGCGG CGATGCCAAT AATCCGATGC TGACCCGCAT CTACGGCACC GCCTTTGCAA ACGATAATGA CCTTCAAGCC TATCTCCATA TGCTTGAGGA AGCGGAGAAG CGCGACCACC GCCGCCTTGG CCGCGAAATG GACCTGTTCC ATTTCCAGGA AGAGGGGCCG GGCGTCGTGT TCTGGCACGC CAAAGGCTGG AAGATGTTCC AGAATCTGGT GAGCTATATG CGCCGCCGCC TCGACAGCCA TGGCTATCAG GAAGTGAATA CGCCGCAGGT GCTGGACAAG TCGCTGTGGG AAACCTCCGG CCACTGGGGC TGGTATCGCG ACAATATGTT CAAGGTTACG GTTGCCGGTG ACGATACCGA CGATGACCGT GTCTTTGCGC TGAAGCCGAT GAACTGCCCC GGCCATGTGC AGATATTCAA GCATGGCCTT AAATCATACC GCGATCTTCC TATAAAGCTT GCGGAATTCG GCAATGTGCA CCGCTATGAG CCTTCCGGTG CGCTGCACGG GCTGATGCGT GTGCGCGGTT TCACGCAGGA CGATGCGCAT ATTTTCTGCA CCGAAGAGCA GATGGCGGCG GAATGCCTGC ACATCAATGA TCTTATCTTG TCGGTCTATA AGGATTTCGG CTTCGAGGAA ATCACCATCA AGCTCTCGAC GCGCCCGGAA AAGCGCGTTG GCTCGGATGA GCTTTGGGAT CGTGCCGAAA GCGTGATGAT GACCGTTCTG GAGCAGATCC GGCAGCAGTC GAACAATATC AAGACCGGCA TTCTGCCGGG CGAGGGTGCG TTCTACGGGC CGAAGTTTGA ATATACGCTG AAGGACGCCA TCGGCCGTGA ATGGCAGTGC GGCACCACGC AGGTCGATTT CAACCTGCCG GAACGCTTCG GCGCCTTCTA TATCGGCGCG GATTCGGAAA AGAAGCAGCC GGTCATGATC CATCGCGCCA TCTGCGGCTC GATGGAACGT TTCCTCGGCA TTCTGATCGA GAATTTTGCC GGTCACATGC CGCTCTGGTT TGCGCCGGTA CAGGTGGTTG TTGCAACCAT CACGTCGGAT GCCGATGAAT ATGCAAAGGA AGCGGCTGCG AAGCTCAAGG CTGCCGGTCT TCAGGTCGTG ACCGATCTGC GCAACGAGAA GATCAACTAC AAGGTTCGCG AACATTCGCT CCAGAAAGTT CCGGTCATCC TCGTTTGCGG CAAGCGTGAA GCAGAAGAAA AGACTGTCAA CATGCGCCGT CTCGGCTCAC GCGATCAGGA ATCCATGACG CTGGATGAAG CGATTGCCCG GCTTTGCGAA GAGGCGACCC CGCCCGATCT GCTTCGCCTG AAAAATGCAG GCAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA
Details for BrabA.00156.a.A1.PW27576
PURIFICATION DATe: 9/8/2009
CONCENTRATION: 3.59mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 39
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSNTVSLQF PDGSVREYDA SMTGAALAES ISKSLAKKAV AYAVDGTVRD LSDPLGASGK LEIITREDPR ALELIRHDTA HVLAEAVQEL FPGTQVTIGP VIENGFYYDF ARNEPFTLDD LPVIEKKMRE IIQRNKPFTK EVWSREKAKQ VFSDKGESYK VELVDAIPAG QDLKIYYQGD WFDLCRGPHM ASTGQIGNSF KLMKVAGAYW RGDANNPMLT RIYGTAFAND NDLQAYLHML EEAEXRDHRR
Validated NT Sequence
tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtcgaa taccgtttcc ctgcaatttc ccgatggttc cgtacgtgaa tacgatgcat cgatgacagg ggccgccctt gcggaatcaa tttccaagtc gcttgccaag aaggctgtcg cttacgctgt tgatggcacg gttcgtgatc tttccgatcc gcttggcgct tccggcaagc tggaaatcat cacccgcgaa gacccgcgcg cgctggaact catccgccac gacactgccc atgtgcttgc agaagccgtg caggagcttt ttcccggaac gcaggtaacg atcggccccg tgatcgaaaa cggcttctat tacgactttg cgcgcaacga gccgtttacg ctcgatgacc tgccggtgat cgaaaagaag atgcgcgaga tcattcagcg caacaagccg ttcaccaagg aagtctggtc acgcgagaag gccaaacagg ttttttccga caagggcgaa agctacaagg tcgagctggt cgatgccatt ccggccgggc aggacctcaa gatctattat cagggtgact ggttcgacct ttgccgcggg ccgcatatgg cttccacggg tcagatcggc aattcattca agttgatgaa ggtggcgggc gcttattggc gcggcgatgc caataatccg atgctgaccc gcatctacgg caccgccttt gcaaacgata atgaccttca agcctatctc catatgcttg aggaagcgga gaancgcgac caccgccgcc tt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSNTVSLQF PDGSVREYDA SMTGAALAES ISKSLAKKAV AYAVDGTVRD LSDPLGASGK LEIITREDPR ALELIRHDTA HVLAEAVQEL FPGTQVTIGP VIENGFYYDF ARNEPFTLDD LPVIEKKMRE IIQRNKPFTK EVWSREKAKQ VFSDKGESYK VELVDAIPAG QDLKIYYQGD WFDLCRGPHM ASTGQIGNSF KLMKVAGAYW RGDANNPMLT RIYGTAFAND NDLQAYLHML EEAEKRDHRR LGREMDLFHF QEEGPGVVFW HAKGWKMFQN LVSYMRRRLD SHGYQEVNTP QVLDKSLWET SGHWGWYRDN MFKVTVAGDD TDDDRVFALK PMNCPGHVQI FKHGLKSYRD LPIKLAEFGN VHRYEPSGAL HGLMRVRGFT QDDAHIFCTE EQMAAECLHI NDLILSVYKD FGFEEITIKL STRPEKRVGS DELWDRAESV MMTVLEQIRQ QSNNIKTGIL PGEGAFYGPK FEYTLKDAIG REWQCGTTQV DFNLPERFGA FYIGADSEKK QPVMIHRAIC GSMERFLGIL IENFAGHMPL WFAPVQVVVA TITSDADEYA KEAAAKLKAA GLQVVTDLRN EKINYKVREH SLQKVPVILV CGKREAEEKT VNMRRLGSRD QESMTLDEAI ARLCEEATPP DLLRLKNAG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCGAATACC GTTTCCCTGC AATTTCCCGA TGGTTCCGTA CGTGAATACG ATGCATCGAT GACAGGGGCC GCCCTTGCGG AATCAATTTC CAAGTCGCTT GCCAAGAAGG CTGTCGCTTA CGCTGTTGAT GGCACGGTTC GTGATCTTTC CGATCCGCTT GGCGCTTCCG GCAAGCTGGA AATCATCACC CGCGAAGACC CGCGCGCGCT GGAACTCATC CGCCACGACA CTGCCCATGT GCTTGCAGAA GCCGTGCAGG AGCTTTTTCC CGGAACGCAG GTAACGATCG GCCCCGTGAT CGAAAACGGC TTCTATTACG ACTTTGCGCG CAACGAGCCG TTTACGCTCG ATGACCTGCC GGTGATCGAA AAGAAGATGC GCGAGATCAT TCAGCGCAAC AAGCCGTTCA CCAAGGAAGT CTGGTCACGC GAGAAGGCCA AACAGGTTTT TTCCGACAAG GGCGAAAGCT ACAAGGTCGA GCTGGTCGAT GCCATTCCGG CCGGGCAGGA CCTCAAGATC TATTATCAGG GTGACTGGTT CGACCTTTGC CGCGGGCCGC ATATGGCTTC CACGGGTCAG ATCGGCAATT CATTCAAGTT GATGAAGGTG GCGGGCGCTT ATTGGCGCGG CGATGCCAAT AATCCGATGC TGACCCGCAT CTACGGCACC GCCTTTGCAA ACGATAATGA CCTTCAAGCC TATCTCCATA TGCTTGAGGA AGCGGAGAAG CGCGACCACC GCCGCCTTGG CCGCGAAATG GACCTGTTCC ATTTCCAGGA AGAGGGGCCG GGCGTCGTGT TCTGGCACGC CAAAGGCTGG AAGATGTTCC AGAATCTGGT GAGCTATATG CGCCGCCGCC TCGACAGCCA TGGCTATCAG GAAGTGAATA CGCCGCAGGT GCTGGACAAG TCGCTGTGGG AAACCTCCGG CCACTGGGGC TGGTATCGCG ACAATATGTT CAAGGTTACG GTTGCCGGTG ACGATACCGA CGATGACCGT GTCTTTGCGC TGAAGCCGAT GAACTGCCCC GGCCATGTGC AGATATTCAA GCATGGCCTT AAATCATACC GCGATCTTCC TATAAAGCTT GCGGAATTCG GCAATGTGCA CCGCTATGAG CCTTCCGGTG CGCTGCACGG GCTGATGCGT GTGCGCGGTT TCACGCAGGA CGATGCGCAT ATTTTCTGCA CCGAAGAGCA GATGGCGGCG GAATGCCTGC ACATCAATGA TCTTATCTTG TCGGTCTATA AGGATTTCGG CTTCGAGGAA ATCACCATCA AGCTCTCGAC GCGCCCGGAA AAGCGCGTTG GCTCGGATGA GCTTTGGGAT CGTGCCGAAA GCGTGATGAT GACCGTTCTG GAGCAGATCC GGCAGCAGTC GAACAATATC AAGACCGGCA TTCTGCCGGG CGAGGGTGCG TTCTACGGGC CGAAGTTTGA ATATACGCTG AAGGACGCCA TCGGCCGTGA ATGGCAGTGC GGCACCACGC AGGTCGATTT CAACCTGCCG GAACGCTTCG GCGCCTTCTA TATCGGCGCG GATTCGGAAA AGAAGCAGCC GGTCATGATC CATCGCGCCA TCTGCGGCTC GATGGAACGT TTCCTCGGCA TTCTGATCGA GAATTTTGCC GGTCACATGC CGCTCTGGTT TGCGCCGGTA CAGGTGGTTG TTGCAACCAT CACGTCGGAT GCCGATGAAT ATGCAAAGGA AGCGGCTGCG AAGCTCAAGG CTGCCGGTCT TCAGGTCGTG ACCGATCTGC GCAACGAGAA GATCAACTAC AAGGTTCGCG AACATTCGCT CCAGAAAGTT CCGGTCATCC TCGTTTGCGG CAAGCGTGAA GCAGAAGAAA AGACTGTCAA CATGCGCCGT CTCGGCTCAC GCGATCAGGA ATCCATGACG CTGGATGAAG CGATTGCCCG GCTTTGCGAA GAGGCGACCC CGCCCGATCT GCTTCGCCTG AAAAATGCAG GCAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA