BrabA.00153.a

DNA primase

CENTER ID: BrabA.00153.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00153.a.B1.GE26953 Full length( BrabA.00153.a ) 1 655
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00153.a.B1.PW26933 Full length( BrabA.00153.a ) 1 655
BrabA.00153.a.B1.PW27475 Full length( BrabA.00153.a ) 1 655

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.1557
RefSeq: YP_414867.1
UniProt: Q2YM25

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRFPPSFLDE IRDRVPISTL IGTRVSFDRK KSNPPKGDFW ACCPFHGEKT PSFHCEDRKG RYHCFGCGVT GDHFKFLTDL EGLSFPEAVE RVADMAGVPM PARAPEMEKR EAQRATLYDV MELAAQFFES QLQSATGAKA RAYLRDRGLS TATQQAFRMG YAPESRNALK EFLAGKGVSR EQIEACGLVV HGEGIAVSYD RFRDRVMFPI EDLRGRIIAF GGRALSADAP AKYLNSPETE LFHKGRVLYN GLRARKACQP QGGEPAKPII AVEGYMDVIA LAQAGIHHVV APLGTALTEE QLELLWRISP EPILCFDGDG AGLRAAYRAA DLGLPVLQPG KSLRFALLPE GQDPDDLVKA EGPSAFHAVL RDARPLVEMV WTRETAGGVF DTPERRAELE ARLREITSRI ANEDIRRHYS QDMRERAQAF FGQGRQGFQN GGQRSQSFTR RNEGARGRGG PAGSGRLAIS DSLARSTLVK GGRQGGAAHA PLRETAIVLT LLNHPRLIEE DFETLAALDL GHDALKTLHL AMLDVLAAGR VDDGIAMRRA LVDAGHGDLI KMLEQVVKGA KLWTATTLAA EDDAREALRQ ALHLHQRART LHKELRAVEA ALETDETGEL FARLRDIQNQ ILKADATEAL IDGFGLSSGR PHGGF
NT Sequence
ATGCGTTTTC CGCCCTCCTT TCTCGATGAG ATCAGAGACC GCGTCCCGAT CTCGACGCTG ATCGGAACGC GTGTTTCATT TGACCGCAAG AAAAGCAATC CGCCCAAGGG CGATTTCTGG GCCTGCTGTC CGTTTCATGG CGAGAAGACG CCGAGCTTTC ATTGCGAGGA CCGCAAGGGC CGTTACCACT GTTTCGGTTG CGGTGTGACT GGCGATCACT TCAAGTTCCT GACAGACCTT GAAGGCTTGA GTTTCCCGGA AGCGGTGGAG CGGGTGGCCG ATATGGCCGG TGTTCCCATG CCCGCGCGCG CCCCGGAAAT GGAAAAGCGC GAGGCGCAGC GCGCAACGCT TTACGATGTG ATGGAACTGG CTGCGCAATT CTTTGAGAGC CAGTTGCAAA GTGCGACCGG TGCGAAAGCG CGCGCCTATC TGCGCGACCG CGGCCTTTCG ACCGCCACGC AGCAGGCGTT CCGCATGGGC TATGCGCCTG AATCACGCAA TGCGCTGAAG GAGTTTCTGG CAGGCAAGGG CGTTTCCCGC GAGCAGATCG AAGCCTGCGG CCTTGTGGTG CATGGCGAGG GAATAGCGGT TTCCTACGAT CGTTTCCGTG ATCGCGTGAT GTTCCCTATC GAGGATTTGC GTGGGCGGAT CATCGCTTTT GGCGGGCGGG CTCTTTCTGC GGATGCGCCT GCGAAATATC TCAATTCGCC CGAAACAGAG CTCTTTCATA AGGGGCGCGT GCTTTATAAC GGCTTGCGGG CGCGCAAGGC CTGCCAACCG CAGGGCGGCG AACCGGCCAA GCCGATCATT GCCGTCGAAG GCTATATGGA CGTCATCGCG CTTGCGCAGG CCGGTATCCA TCATGTGGTG GCTCCGCTTG GCACTGCTCT GACCGAAGAA CAGCTTGAAC TTCTCTGGCG CATCAGCCCC GAGCCGATCC TCTGTTTTGA CGGTGACGGC GCTGGCCTTC GTGCGGCCTA TCGTGCTGCC GATCTTGGCC TGCCTGTCTT GCAACCCGGC AAGTCGCTGC GCTTTGCGCT TTTGCCGGAA GGGCAGGACC CGGACGATCT CGTCAAGGCG GAAGGTCCGT CCGCTTTTCA TGCGGTTCTG CGCGATGCAA GACCGCTGGT GGAGATGGTC TGGACGCGGG AAACGGCAGG CGGGGTTTTC GATACGCCGG AGCGCCGGGC CGAACTGGAG GCGCGTCTGC GCGAAATTAC GAGCCGCATT GCCAATGAAG ATATCCGCCG CCACTACAGC CAGGATATGC GCGAGCGGGC GCAGGCCTTT TTCGGGCAGG GGCGGCAGGG CTTTCAAAAC GGCGGCCAGC GCAGCCAGTC GTTTACGCGC CGCAATGAAG GCGCGCGCGG ACGCGGCGGC CCGGCCGGCA GCGGCCGGCT GGCAATCTCC GACAGTCTCG CCCGGTCGAC GCTGGTAAAG GGCGGCCGGC AGGGTGGTGC TGCACATGCT CCCTTGCGCG AGACGGCCAT CGTGCTGACG CTTCTCAATC ATCCCCGGCT TATCGAGGAG GATTTCGAGA CGCTTGCCGC GCTTGATCTT GGCCATGATG CCTTGAAGAC GCTGCATCTT GCCATGCTGG ATGTGCTGGC GGCGGGACGT GTGGACGACG GGATCGCCAT GCGGCGCGCG CTGGTGGATG CCGGTCATGG CGATCTGATC AAGATGCTGG AGCAGGTGGT GAAGGGCGCG AAACTGTGGA CCGCGACCAC CCTTGCCGCT GAAGATGATG CGCGTGAAGC ACTGCGTCAG GCGCTTCACT TGCATCAGCG CGCGCGTACA CTACATAAGG AACTGCGCGC TGTTGAGGCC GCACTTGAGA CAGATGAGAC GGGCGAGTTG TTCGCGCGTC TTCGCGATAT ACAAAACCAG ATTTTAAAAG CCGATGCGAC CGAGGCCCTG ATAGATGGAT TTGGCCTGTC ATCGGGGCGG CCACATGGCG GTTTC
Details for BrabA.00153.a.B1.GE26953
HARVESTED ON: 6/18/2009
SEQUENCED ON: 7/15/2009
EXPECTED MW: 72kDa
OBSERVED MW: 72kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 35
Validated AA Sequence
MRERAQXFFG QGRQGFQNGG QRSQSFTRRX EGARGRGGPA GSGRLAISDS LARSTLVKGG RQGGAAHAPL RETAIVLTLL NHPRLIEEDF ETLAALDLGH DALKTLHLAM LDVLAAGRVD DGIAMRRALV DAGHGDLIKM LEQVVKGAKL WTATTLAAED DAREALRQAL HLHQRARTLH KELRAVEAAL ETDETGELFA RLRDIQNQIL KADATEALID GFGLSSGRPH GGF
Validated NT Sequence
atcttactca cttagaaacc gccatgtggc cgccccgatg acaggccaaa tccatctatc agggcctcgg tcgcatcggc ttttaaaatc tggttttgta tatcgcgaag acgcgcgaac aactcgcccg tctcatctgt ctcaagtgcg gcctcaacag cgcgcagttc cttatgtagt gtacgcgcgc gctgatgcaa gtgaagcgcc tgacgcagtg cttcacgcgc atcatcttca gcggcaaggg tggtcgcggt ccacagtttc gcgcccttca ccacctgctc cagcatcttg atcagatcgc catgaccggc atccaccagc gcgcgccgca tggcgatccc gtcgtccaca cgtcccgccg ccagcacatc cagcatggca agatgcagcg tcttcaaggc atcatggcca agatcaagcg cggcaagcgt ctcgaaatcc tcctcgataa gccggggatg attgagaagc gtcagcacga tggccgtctc gcgcaaggga gcatgtgcag caccaccctg ccggccgccc tttaccagcg tcgaccgggc gagactgtcg gagattgcca gccggccgct gccggccggg ccgccgcgtc cgcgcgcgcc ttcnttgcgg cgcgtaaacg actggctgcg ctggccgccg ttttgaaagc cctgccgccc ctgcccgaaa aaggnctgcg cccgctcgcg catatcctgg ctgtagtgnc ggcgganatc ttcattggca atgcggctcg taatttcgcg canacgcgcc tccngttcgg nccggcgctc cngcgtatcn aaaancccgc ctgccgttnc ccgcgtccag annatctcca ccagcggtct tgcatcgcgc anaaccgcat gaaaagcgga
Expected Protein Sequence
MAHHHHHHMR FPPSFLDEIR DRVPISTLIG TRVSFDRKKS NPPKGDFWAC CPFHGEKTPS FHCEDRKGRY HCFGCGVTGD HFKFLTDLEG LSFPEAVERV ADMAGVPMPA RAPEMEKREA QRATLYDVME LAAQFFESQL QSATGAKARA YLRDRGLSTA TQQAFRMGYA PESRNALKEF LAGKGVSREQ IEACGLVVHG EGIAVSYDRF RDRVMFPIED LRGRIIAFGG RALSADAPAK YLNSPETELF HKGRVLYNGL RARKACQPQG GEPAKPIIAV EGYMDVIALA QAGIHHVVAP LGTALTEEQL ELLWRISPEP ILCFDGDGAG LRAAYRAADL GLPVLQPGKS LRFALLPEGQ DPDDLVKAEG PSAFHAVLRD ARPLVEMVWT RETAGGVFDT PERRAELEAR LREITSRIAN EDIRRHYSQD MRERAQAFFG QGRQGFQNGG QRSQSFTRRN EGARGRGGPA GSGRLAISDS LARSTLVKGG RQGGAAHAPL RETAIVLTLL NHPRLIEEDF ETLAALDLGH DALKTLHLAM LDVLAAGRVD DGIAMRRALV DAGHGDLIKM LEQVVKGAKL WTATTLAAED DAREALRQAL HLHQRARTLH KELRAVEAAL ETDETGELFA RLRDIQNQIL KADATEALID GFGLSSGRPH GGF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtt ttccgccctc ctttctcgat gagatcagag accgcgtccc gatctcgacg ctgatcggaa cgcgtgtttc atttgaccgc aagaaaagca atccgcccaa gggcgatttc tgggcctgct gtccgtttca tggcgagaag acgccgagct ttcattgcga ggaccgcaag ggccgttacc actgtttcgg ttgcggtgtg actggcgatc acttcaagtt cctgacagac cttgaaggct tgagtttccc ggaagcggtg gagcgggtgg ccgatatggc cggtgttccc atgcccgcgc gcgccccgga aatggaaaag cgcgaggcgc agcgcgcaac gctttacgat gtgatggaac tggctgcgca attctttgag agccagttgc aaagtgcgac cggtgcgaaa gcgcgcgcct atctgcgcga ccgcggcctt tcgaccgcca cgcagcaggc gttccgcatg ggctatgcgc ctgaatcacg caatgcgctg aaggagtttc tggcaggcaa gggcgtttcc cgcgagcaga tcgaagcctg cggccttgtg gtgcatggcg agggaatagc ggtttcctac gatcgtttcc gtgatcgcgt gatgttccct atcgaggatt tgcgtgggcg gatcatcgct tttggcgggc gggctctttc tgcggatgcg cctgcgaaat atctcaattc gcccgaaaca gagctctttc ataaggggcg cgtgctttat aacggcttgc gggcgcgcaa ggcctgccaa ccgcagggcg gcgaaccggc caagccgatc attgccgtcg aaggctatat ggacgtcatc gcgcttgcgc aggccggtat ccatcatgtg gtggctccgc ttggcactgc tctgaccgaa gaacagcttg aacttctctg gcgcatcagc cccgagccga tcctctgttt tgacggtgac ggcgctggcc ttcgtgcggc ctatcgtgct gccgatcttg gcctgcctgt cttgcaaccc ggcaagtcgc tgcgctttgc gcttttgccg gaagggcagg acccggacga tctcgtcaag gcggaaggtc cgtccgcttt tcatgcggtt ctgcgcgatg caagaccgct ggtggagatg gtctggacgc gggaaacggc aggcggggtt ttcgatacgc cggagcgccg ggccgaactg gaggcgcgtc tgcgcgaaat tacgagccgc attgccaatg aagatatccg ccgccactac agccaggata tgcgcgagcg ggcgcaggcc tttttcgggc aggggcggca gggctttcaa aacggcggcc agcgcagcca gtcgtttacg cgccgcaatg aaggcgcgcg cggacgcggc ggcccggccg gcagcggccg gctggcaatc tccgacagtc tcgcccggtc gacgctggta aagggcggcc ggcagggtgg tgctgcacat gctcccttgc gcgagacggc catcgtgctg acgcttctca atcatccccg gcttatcgag gaggatttcg agacgcttgc cgcgcttgat cttggccatg atgccttgaa gacgctgcat cttgccatgc tggatgtgct ggcggcggga cgtgtggacg acgggatcgc catgcggcgc gcgctggtgg atgccggtca tggcgatctg atcaagatgc tggagcaggt ggtgaagggc gcgaaactgt ggaccgcgac cacccttgcc gctgaagatg atgcgcgtga agcactgcgt caggcgcttc acttgcatca gcgcgcgcgt acactacata aggaactgcg cgctgttgag gccgcacttg agacagatga gacgggcgag ttgttcgcgc gtcttcgcga tatacaaaac cagattttaa aagccgatgc gaccgaggcc ctgatagatg gatttggcct gtcatcgggg cggccacatg gcggtttctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BrabA.00153.a.B1.PW26933
PURIFICATION DATe: 8/4/2009
CONCENTRATION: 29.09mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 13
VIAL VOLUME: 100µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 35
Protocol Notes
notes unavailable
Validated AA Sequence
MRERAQXFFG QGRQGFQNGG QRSQSFTRRX EGARGRGGPA GSGRLAISDS LARSTLVKGG RQGGAAHAPL RETAIVLTLL NHPRLIEEDF ETLAALDLGH DALKTLHLAM LDVLAAGRVD DGIAMRRALV DAGHGDLIKM LEQVVKGAKL WTATTLAAED DAREALRQAL HLHQRARTLH KELRAVEAAL ETDETGELFA RLRDIQNQIL KADATEALID GFGLSSGRPH GGF
Validated NT Sequence
atcttactca cttagaaacc gccatgtggc cgccccgatg acaggccaaa tccatctatc agggcctcgg tcgcatcggc ttttaaaatc tggttttgta tatcgcgaag acgcgcgaac aactcgcccg tctcatctgt ctcaagtgcg gcctcaacag cgcgcagttc cttatgtagt gtacgcgcgc gctgatgcaa gtgaagcgcc tgacgcagtg cttcacgcgc atcatcttca gcggcaaggg tggtcgcggt ccacagtttc gcgcccttca ccacctgctc cagcatcttg atcagatcgc catgaccggc atccaccagc gcgcgccgca tggcgatccc gtcgtccaca cgtcccgccg ccagcacatc cagcatggca agatgcagcg tcttcaaggc atcatggcca agatcaagcg cggcaagcgt ctcgaaatcc tcctcgataa gccggggatg attgagaagc gtcagcacga tggccgtctc gcgcaaggga gcatgtgcag caccaccctg ccggccgccc tttaccagcg tcgaccgggc gagactgtcg gagattgcca gccggccgct gccggccggg ccgccgcgtc cgcgcgcgcc ttcnttgcgg cgcgtaaacg actggctgcg ctggccgccg ttttgaaagc cctgccgccc ctgcccgaaa aaggnctgcg cccgctcgcg catatcctgg ctgtagtgnc ggcgganatc ttcattggca atgcggctcg taatttcgcg canacgcgcc tccngttcgg nccggcgctc cngcgtatcn aaaancccgc ctgccgttnc ccgcgtccag annatctcca ccagcggtct tgcatcgcgc anaaccgcat gaaaagcgga
Expressed Protein Sequence
MAHHHHHHMR FPPSFLDEIR DRVPISTLIG TRVSFDRKKS NPPKGDFWAC CPFHGEKTPS FHCEDRKGRY HCFGCGVTGD HFKFLTDLEG LSFPEAVERV ADMAGVPMPA RAPEMEKREA QRATLYDVME LAAQFFESQL QSATGAKARA YLRDRGLSTA TQQAFRMGYA PESRNALKEF LAGKGVSREQ IEACGLVVHG EGIAVSYDRF RDRVMFPIED LRGRIIAFGG RALSADAPAK YLNSPETELF HKGRVLYNGL RARKACQPQG GEPAKPIIAV EGYMDVIALA QAGIHHVVAP LGTALTEEQL ELLWRISPEP ILCFDGDGAG LRAAYRAADL GLPVLQPGKS LRFALLPEGQ DPDDLVKAEG PSAFHAVLRD ARPLVEMVWT RETAGGVFDT PERRAELEAR LREITSRIAN EDIRRHYSQD MRERAQAFFG QGRQGFQNGG QRSQSFTRRN EGARGRGGPA GSGRLAISDS LARSTLVKGG RQGGAAHAPL RETAIVLTLL NHPRLIEEDF ETLAALDLGH DALKTLHLAM LDVLAAGRVD DGIAMRRALV DAGHGDLIKM LEQVVKGAKL WTATTLAAED DAREALRQAL HLHQRARTLH KELRAVEAAL ETDETGELFA RLRDIQNQIL KADATEALID GFGLSSGRPH GGF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGTT TTCCGCCCTC CTTTCTCGAT GAGATCAGAG ACCGCGTCCC GATCTCGACG CTGATCGGAA CGCGTGTTTC ATTTGACCGC AAGAAAAGCA ATCCGCCCAA GGGCGATTTC TGGGCCTGCT GTCCGTTTCA TGGCGAGAAG ACGCCGAGCT TTCATTGCGA GGACCGCAAG GGCCGTTACC ACTGTTTCGG TTGCGGTGTG ACTGGCGATC ACTTCAAGTT CCTGACAGAC CTTGAAGGCT TGAGTTTCCC GGAAGCGGTG GAGCGGGTGG CCGATATGGC CGGTGTTCCC ATGCCCGCGC GCGCCCCGGA AATGGAAAAG CGCGAGGCGC AGCGCGCAAC GCTTTACGAT GTGATGGAAC TGGCTGCGCA ATTCTTTGAG AGCCAGTTGC AAAGTGCGAC CGGTGCGAAA GCGCGCGCCT ATCTGCGCGA CCGCGGCCTT TCGACCGCCA CGCAGCAGGC GTTCCGCATG GGCTATGCGC CTGAATCACG CAATGCGCTG AAGGAGTTTC TGGCAGGCAA GGGCGTTTCC CGCGAGCAGA TCGAAGCCTG CGGCCTTGTG GTGCATGGCG AGGGAATAGC GGTTTCCTAC GATCGTTTCC GTGATCGCGT GATGTTCCCT ATCGAGGATT TGCGTGGGCG GATCATCGCT TTTGGCGGGC GGGCTCTTTC TGCGGATGCG CCTGCGAAAT ATCTCAATTC GCCCGAAACA GAGCTCTTTC ATAAGGGGCG CGTGCTTTAT AACGGCTTGC GGGCGCGCAA GGCCTGCCAA CCGCAGGGCG GCGAACCGGC CAAGCCGATC ATTGCCGTCG AAGGCTATAT GGACGTCATC GCGCTTGCGC AGGCCGGTAT CCATCATGTG GTGGCTCCGC TTGGCACTGC TCTGACCGAA GAACAGCTTG AACTTCTCTG GCGCATCAGC CCCGAGCCGA TCCTCTGTTT TGACGGTGAC GGCGCTGGCC TTCGTGCGGC CTATCGTGCT GCCGATCTTG GCCTGCCTGT CTTGCAACCC GGCAAGTCGC TGCGCTTTGC GCTTTTGCCG GAAGGGCAGG ACCCGGACGA TCTCGTCAAG GCGGAAGGTC CGTCCGCTTT TCATGCGGTT CTGCGCGATG CAAGACCGCT GGTGGAGATG GTCTGGACGC GGGAAACGGC AGGCGGGGTT TTCGATACGC CGGAGCGCCG GGCCGAACTG GAGGCGCGTC TGCGCGAAAT TACGAGCCGC ATTGCCAATG AAGATATCCG CCGCCACTAC AGCCAGGATA TGCGCGAGCG GGCGCAGGCC TTTTTCGGGC AGGGGCGGCA GGGCTTTCAA AACGGCGGCC AGCGCAGCCA GTCGTTTACG CGCCGCAATG AAGGCGCGCG CGGACGCGGC GGCCCGGCCG GCAGCGGCCG GCTGGCAATC TCCGACAGTC TCGCCCGGTC GACGCTGGTA AAGGGCGGCC GGCAGGGTGG TGCTGCACAT GCTCCCTTGC GCGAGACGGC CATCGTGCTG ACGCTTCTCA ATCATCCCCG GCTTATCGAG GAGGATTTCG AGACGCTTGC CGCGCTTGAT CTTGGCCATG ATGCCTTGAA GACGCTGCAT CTTGCCATGC TGGATGTGCT GGCGGCGGGA CGTGTGGACG ACGGGATCGC CATGCGGCGC GCGCTGGTGG ATGCCGGTCA TGGCGATCTG ATCAAGATGC TGGAGCAGGT GGTGAAGGGC GCGAAACTGT GGACCGCGAC CACCCTTGCC GCTGAAGATG ATGCGCGTGA AGCACTGCGT CAGGCGCTTC ACTTGCATCA GCGCGCGCGT ACACTACATA AGGAACTGCG CGCTGTTGAG GCCGCACTTG AGACAGATGA GACGGGCGAG TTGTTCGCGC GTCTTCGCGA TATACAAAAC CAGATTTTAA AAGCCGATGC GACCGAGGCC CTGATAGATG GATTTGGCCT GTCATCGGGG CGGCCACATG GCGGTTTCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT
Details for BrabA.00153.a.B1.PW27475
PURIFICATION DATe: 8/4/2009
CONCENTRATION: 18.72mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 19
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 35
Protocol Notes
notes unavailable
Validated AA Sequence
MRERAQXFFG QGRQGFQNGG QRSQSFTRRX EGARGRGGPA GSGRLAISDS LARSTLVKGG RQGGAAHAPL RETAIVLTLL NHPRLIEEDF ETLAALDLGH DALKTLHLAM LDVLAAGRVD DGIAMRRALV DAGHGDLIKM LEQVVKGAKL WTATTLAAED DAREALRQAL HLHQRARTLH KELRAVEAAL ETDETGELFA RLRDIQNQIL KADATEALID GFGLSSGRPH GGF
Validated NT Sequence
atcttactca cttagaaacc gccatgtggc cgccccgatg acaggccaaa tccatctatc agggcctcgg tcgcatcggc ttttaaaatc tggttttgta tatcgcgaag acgcgcgaac aactcgcccg tctcatctgt ctcaagtgcg gcctcaacag cgcgcagttc cttatgtagt gtacgcgcgc gctgatgcaa gtgaagcgcc tgacgcagtg cttcacgcgc atcatcttca gcggcaaggg tggtcgcggt ccacagtttc gcgcccttca ccacctgctc cagcatcttg atcagatcgc catgaccggc atccaccagc gcgcgccgca tggcgatccc gtcgtccaca cgtcccgccg ccagcacatc cagcatggca agatgcagcg tcttcaaggc atcatggcca agatcaagcg cggcaagcgt ctcgaaatcc tcctcgataa gccggggatg attgagaagc gtcagcacga tggccgtctc gcgcaaggga gcatgtgcag caccaccctg ccggccgccc tttaccagcg tcgaccgggc gagactgtcg gagattgcca gccggccgct gccggccggg ccgccgcgtc cgcgcgcgcc ttcnttgcgg cgcgtaaacg actggctgcg ctggccgccg ttttgaaagc cctgccgccc ctgcccgaaa aaggnctgcg cccgctcgcg catatcctgg ctgtagtgnc ggcgganatc ttcattggca atgcggctcg taatttcgcg canacgcgcc tccngttcgg nccggcgctc cngcgtatcn aaaancccgc ctgccgttnc ccgcgtccag annatctcca ccagcggtct tgcatcgcgc anaaccgcat gaaaagcgga
Expressed Protein Sequence
MAHHHHHHMR FPPSFLDEIR DRVPISTLIG TRVSFDRKKS NPPKGDFWAC CPFHGEKTPS FHCEDRKGRY HCFGCGVTGD HFKFLTDLEG LSFPEAVERV ADMAGVPMPA RAPEMEKREA QRATLYDVME LAAQFFESQL QSATGAKARA YLRDRGLSTA TQQAFRMGYA PESRNALKEF LAGKGVSREQ IEACGLVVHG EGIAVSYDRF RDRVMFPIED LRGRIIAFGG RALSADAPAK YLNSPETELF HKGRVLYNGL RARKACQPQG GEPAKPIIAV EGYMDVIALA QAGIHHVVAP LGTALTEEQL ELLWRISPEP ILCFDGDGAG LRAAYRAADL GLPVLQPGKS LRFALLPEGQ DPDDLVKAEG PSAFHAVLRD ARPLVEMVWT RETAGGVFDT PERRAELEAR LREITSRIAN EDIRRHYSQD MRERAQAFFG QGRQGFQNGG QRSQSFTRRN EGARGRGGPA GSGRLAISDS LARSTLVKGG RQGGAAHAPL RETAIVLTLL NHPRLIEEDF ETLAALDLGH DALKTLHLAM LDVLAAGRVD DGIAMRRALV DAGHGDLIKM LEQVVKGAKL WTATTLAAED DAREALRQAL HLHQRARTLH KELRAVEAAL ETDETGELFA RLRDIQNQIL KADATEALID GFGLSSGRPH GGF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGTT TTCCGCCCTC CTTTCTCGAT GAGATCAGAG ACCGCGTCCC GATCTCGACG CTGATCGGAA CGCGTGTTTC ATTTGACCGC AAGAAAAGCA ATCCGCCCAA GGGCGATTTC TGGGCCTGCT GTCCGTTTCA TGGCGAGAAG ACGCCGAGCT TTCATTGCGA GGACCGCAAG GGCCGTTACC ACTGTTTCGG TTGCGGTGTG ACTGGCGATC ACTTCAAGTT CCTGACAGAC CTTGAAGGCT TGAGTTTCCC GGAAGCGGTG GAGCGGGTGG CCGATATGGC CGGTGTTCCC ATGCCCGCGC GCGCCCCGGA AATGGAAAAG CGCGAGGCGC AGCGCGCAAC GCTTTACGAT GTGATGGAAC TGGCTGCGCA ATTCTTTGAG AGCCAGTTGC AAAGTGCGAC CGGTGCGAAA GCGCGCGCCT ATCTGCGCGA CCGCGGCCTT TCGACCGCCA CGCAGCAGGC GTTCCGCATG GGCTATGCGC CTGAATCACG CAATGCGCTG AAGGAGTTTC TGGCAGGCAA GGGCGTTTCC CGCGAGCAGA TCGAAGCCTG CGGCCTTGTG GTGCATGGCG AGGGAATAGC GGTTTCCTAC GATCGTTTCC GTGATCGCGT GATGTTCCCT ATCGAGGATT TGCGTGGGCG GATCATCGCT TTTGGCGGGC GGGCTCTTTC TGCGGATGCG CCTGCGAAAT ATCTCAATTC GCCCGAAACA GAGCTCTTTC ATAAGGGGCG CGTGCTTTAT AACGGCTTGC GGGCGCGCAA GGCCTGCCAA CCGCAGGGCG GCGAACCGGC CAAGCCGATC ATTGCCGTCG AAGGCTATAT GGACGTCATC GCGCTTGCGC AGGCCGGTAT CCATCATGTG GTGGCTCCGC TTGGCACTGC TCTGACCGAA GAACAGCTTG AACTTCTCTG GCGCATCAGC CCCGAGCCGA TCCTCTGTTT TGACGGTGAC GGCGCTGGCC TTCGTGCGGC CTATCGTGCT GCCGATCTTG GCCTGCCTGT CTTGCAACCC GGCAAGTCGC TGCGCTTTGC GCTTTTGCCG GAAGGGCAGG ACCCGGACGA TCTCGTCAAG GCGGAAGGTC CGTCCGCTTT TCATGCGGTT CTGCGCGATG CAAGACCGCT GGTGGAGATG GTCTGGACGC GGGAAACGGC AGGCGGGGTT TTCGATACGC CGGAGCGCCG GGCCGAACTG GAGGCGCGTC TGCGCGAAAT TACGAGCCGC ATTGCCAATG AAGATATCCG CCGCCACTAC AGCCAGGATA TGCGCGAGCG GGCGCAGGCC TTTTTCGGGC AGGGGCGGCA GGGCTTTCAA AACGGCGGCC AGCGCAGCCA GTCGTTTACG CGCCGCAATG AAGGCGCGCG CGGACGCGGC GGCCCGGCCG GCAGCGGCCG GCTGGCAATC TCCGACAGTC TCGCCCGGTC GACGCTGGTA AAGGGCGGCC GGCAGGGTGG TGCTGCACAT GCTCCCTTGC GCGAGACGGC CATCGTGCTG ACGCTTCTCA ATCATCCCCG GCTTATCGAG GAGGATTTCG AGACGCTTGC CGCGCTTGAT CTTGGCCATG ATGCCTTGAA GACGCTGCAT CTTGCCATGC TGGATGTGCT GGCGGCGGGA CGTGTGGACG ACGGGATCGC CATGCGGCGC GCGCTGGTGG ATGCCGGTCA TGGCGATCTG ATCAAGATGC TGGAGCAGGT GGTGAAGGGC GCGAAACTGT GGACCGCGAC CACCCTTGCC GCTGAAGATG ATGCGCGTGA AGCACTGCGT CAGGCGCTTC ACTTGCATCA GCGCGCGCGT ACACTACATA AGGAACTGCG CGCTGTTGAG GCCGCACTTG AGACAGATGA GACGGGCGAG TTGTTCGCGC GTCTTCGCGA TATACAAAAC CAGATTTTAA AAGCCGATGC GACCGAGGCC CTGATAGATG GATTTGGCCT GTCATCGGGG CGGCCACATG GCGGTTTCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT