BrabA.00150.a

Bacterial transferase hexapeptide repeat:ATP/GTP-binding site motif A (P-loop):Nucleotidyl transferase

CENTER ID: BrabA.00150.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00150.a.A1.PS00111 Full length( BrabA.00150.a ) 1 454

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.2951
RefSeq: YP_418832.1
UniProt: Q2YKK2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDRTCLSIV LAAGEGTRMK SNLPKVLHRV AGLPLVCHVV NAVRGTGKSD VALVVGRGAE DVRSAVEKIA GPVSAFEQKE RLGTAHAVLA AHEAIARGYD DLLIVFGDTP LIEAQSLLAA RERLAQGADL VVIGFRPASP HGYGRLIEEG GQLVAIIEEK EATDEQKKIG FCNGGLMALR GQHALALLDA VGNDNAKGEY YLTDIVAIAH GKGLNVTAIE VPVDNVIGIN NRAELAEAET IWQNRKRREL MLSGVTLIAP ETVFFSYDTV IEPDVVIEPN VFFGPSVHVA SGALIHSFSH LEGAQVGEKA EIGPFARLRP GADLAEKSKV GNFCEVKNAK VGKGAKINHL AYIGDAVIGA SSNIGAGTIT CNYDGYNKFK TIIGDNAFIG SNSSLVAPVE IGDNAYIASG SVITADVPAD ALALGRARQE TKEGRAKILR EKYAAIKAAK SVSK
NT Sequence
ATGACTGACC GTACATGCCT TTCCATCGTG CTTGCCGCTG GCGAAGGCAC GCGCATGAAA TCGAATTTGC CGAAAGTGCT GCATCGCGTG GCAGGCCTGC CGCTGGTATG CCATGTGGTC AATGCGGTGC GGGGCACGGG CAAAAGCGAC GTTGCGCTTG TGGTCGGCCG CGGTGCGGAA GACGTGCGCA GCGCGGTGGA GAAAATCGCC GGGCCGGTTT CGGCCTTCGA GCAGAAGGAA AGGCTTGGCA CGGCGCACGC TGTTCTTGCC GCGCACGAAG CCATTGCGCG TGGCTATGAC GACCTTCTGA TCGTTTTTGG CGATACGCCG CTGATCGAGG CGCAATCGCT TCTGGCCGCG CGTGAGCGGC TGGCGCAAGG GGCAGACCTT GTCGTGATCG GTTTTCGCCC GGCCAGCCCG CACGGCTATG GCCGCCTGAT CGAAGAAGGC GGGCAGCTTG TCGCGATTAT CGAGGAAAAA GAGGCGACCG ACGAACAGAA GAAAATCGGC TTCTGCAATG GCGGCCTCAT GGCGTTGCGC GGACAACACG CGCTGGCGCT GCTCGATGCC GTTGGCAATG ACAATGCGAA GGGCGAATAT TACCTCACTG ATATTGTTGC AATCGCGCAC GGCAAGGGGC TGAACGTCAC GGCCATCGAA GTGCCGGTCG ATAATGTCAT CGGCATCAAC AATCGCGCCG AACTGGCCGA GGCCGAAACC ATCTGGCAAA ACCGCAAGCG TCGGGAGCTT ATGCTTTCCG GTGTGACGCT GATCGCACCT GAAACAGTCT TCTTTTCATA TGACACGGTG ATTGAGCCCG ATGTTGTGAT CGAACCGAAC GTCTTCTTCG GCCCTTCGGT GCATGTCGCA TCGGGTGCGC TCATCCATTC CTTCTCGCAT CTGGAAGGGG CGCAGGTGGG TGAAAAGGCC GAGATCGGGC CATTTGCCCG GCTTCGCCCC GGCGCCGATC TGGCGGAAAA ATCCAAGGTC GGCAATTTCT GCGAAGTAAA GAACGCGAAG GTTGGCAAGG GCGCAAAGAT CAATCACCTG GCCTATATTG GCGATGCGGT CATCGGCGCT TCCAGCAATA TTGGTGCGGG CACGATCACC TGTAATTATG ATGGCTACAA CAAGTTCAAG ACCATCATCG GCGACAATGC CTTTATCGGC TCCAACAGTT CGCTCGTTGC GCCGGTGGAA ATCGGCGACA ACGCCTATAT CGCCTCCGGC AGCGTCATCA CGGCAGACGT TCCAGCCGAT GCGCTGGCCC TTGGCCGCGC CCGTCAGGAG ACGAAGGAAG GCCGGGCAAA GATCCTGCGG GAGAAGTATG CCGCCATCAA AGCGGCTAAA AGCGTTTCAA AG
Details for BrabA.00150.a.A1.PS00111
PURIFICATION DATe: 10/23/2008
CONCENTRATION: 23.5mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDRTCLSI VLAAGEGTRM KSNLPKVLHR VAGLPLVCHV VNAVRGTGKS DVALVVGRGA EDVRSAVEKI AGPVSAFEQK ERLGTAHAVL AAHEAIARGY DDLLIVFGDT PLIEAQSLLA ARERLAQGAD LVVIGFRPAS PHGYGRLIEE GGQLVAIIEE KEATDEQKKI GFCNGGLMAL RGQHALALLD AVGNDNAKGE YYLTDIVAIA HGKGLNVTAI EVPVDNVIGI NNRAELAEAE TIWQNRKRRE LMLSGVTLIA PETVFFSYDT VIEPDVVIEP NVFFGPSVHV ASGALIHSFS HLEGAQVGEK AEIGPFARLR PGADLAEKSK VGNFCEVKNA KVGKGAKINH LAYIGDAVIG ASSNIGAGTI TCNYDGYNKF KTIIGDNAFI GSNSSLVAPV EIGDNAYIAS GSVITADVPA DALALGRARQ ETKEGRAKIL REKYAAIKAA KSVSK
Validated NT Sequence
ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgact gaccgtacat gcctttccat cgtgcttgcc gctggcgaag gcacgcgcat gaaatcgaat ttgccgaaag tgctgcatcg cgtggcaggc ctgccgctgg tatgccatgt ggtcaatgcg gtgcggggca cgggcaaaag cgacgttgcg cttgtggtcg gccgcggtgc ggaagacgtg cgcagcgcgg tggagaaaat cgccgggccg gtttcggcct tcgagcagaa ggaaaggctt ggcacggcgc acgctgttct tgccgcgcac gaagccattg cgcgtggcta tgacgacctt ctgatcgttt ttggcgatac gccgctgatc gaggcgcaat cgcttctggc cgcgcgtgag cggctggcgc aaggggcaga ccttgtcgtg atcggttttc gcccggccag cccgcacggc tatggccgcc tgatcgaaga aggcgggcag cttgtcgcga ttatcgagga aaaagaggcg accgacgaac agaagaaaat cggcttctgc aatggcggcc tcatggcgtt gcgcggacaa cacgcgctgg cgctgctcga tgccgttggc aatgacaatg cgaagggcga atattacctc actgatattg ttgcaatcgc gcacggcaag gggctgaacg tcacggccat cgaagtgccg gtcgataatg tcatcggcat caacaatcgc gccgaactgg ccgaggccga aaccatctgg caaaaccgca agcgtcggga gcttatgctt tccggtgtga cgctgatcgc acctgaaaca gtcttctttt catatgacac ggtgattgag cccgatgttg tgatcgaacc gaacgtcttc ttcggccctt cggtgcatgt cgcatcgggt gcgctcatcc attccttctc gcatctggaa ggggcgcagg tgggtgaaaa ggccgagatc gggccatttg cccggcttcg ccccggcgcc gatctggcgg aaaaatccaa ggtcggcaat ttctgcgaag taaagaacgc gaaggttggc aagggcgcaa agatcaatca cctggcctat attggcgatg cggtcatcgg cgcttccagc aatattggtg cgggcacgat cacctgtaac tatgatggct acaacaagtt caagaccatc atcggcgaca atgcctttat cggctccaac agttcgctcg ttgcgccggt ggaaatcggc gacaacgcct atatcgcctc cggcagcgtc atcacggcag acgttccagc cgatgcgctg gcccttggcc gcgcccgtca ggagacgaag gaaggccggg caaagatcct gcgggagaag tatgccgcca tcaaagcggc taaaagcgtt tcaaagtaat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDRTCLSI VLAAGEGTRM KSNLPKVLHR VAGLPLVCHV VNAVRGTGKS DVALVVGRGA EDVRSAVEKI AGPVSAFEQK ERLGTAHAVL AAHEAIARGY DDLLIVFGDT PLIEAQSLLA ARERLAQGAD LVVIGFRPAS PHGYGRLIEE GGQLVAIIEE KEATDEQKKI GFCNGGLMAL RGQHALALLD AVGNDNAKGE YYLTDIVAIA HGKGLNVTAI EVPVDNVIGI NNRAELAEAE TIWQNRKRRE LMLSGVTLIA PETVFFSYDT VIEPDVVIEP NVFFGPSVHV ASGALIHSFS HLEGAQVGEK AEIGPFARLR PGADLAEKSK VGNFCEVKNA KVGKGAKINH LAYIGDAVIG ASSNIGAGTI TCNYDGYNKF KTIIGDNAFI GSNSSLVAPV EIGDNAYIAS GSVITADVPA DALALGRARQ ETKEGRAKIL REKYAAIKAA KSVSK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACTGACCGT ACATGCCTTT CCATCGTGCT TGCCGCTGGC GAAGGCACGC GCATGAAATC GAATTTGCCG AAAGTGCTGC ATCGCGTGGC AGGCCTGCCG CTGGTATGCC ATGTGGTCAA TGCGGTGCGG GGCACGGGCA AAAGCGACGT TGCGCTTGTG GTCGGCCGCG GTGCGGAAGA CGTGCGCAGC GCGGTGGAGA AAATCGCCGG GCCGGTTTCG GCCTTCGAGC AGAAGGAAAG GCTTGGCACG GCGCACGCTG TTCTTGCCGC GCACGAAGCC ATTGCGCGTG GCTATGACGA CCTTCTGATC GTTTTTGGCG ATACGCCGCT GATCGAGGCG CAATCGCTTC TGGCCGCGCG TGAGCGGCTG GCGCAAGGGG CAGACCTTGT CGTGATCGGT TTTCGCCCGG CCAGCCCGCA CGGCTATGGC CGCCTGATCG AAGAAGGCGG GCAGCTTGTC GCGATTATCG AGGAAAAAGA GGCGACCGAC GAACAGAAGA AAATCGGCTT CTGCAATGGC GGCCTCATGG CGTTGCGCGG ACAACACGCG CTGGCGCTGC TCGATGCCGT TGGCAATGAC AATGCGAAGG GCGAATATTA CCTCACTGAT ATTGTTGCAA TCGCGCACGG CAAGGGGCTG AACGTCACGG CCATCGAAGT GCCGGTCGAT AATGTCATCG GCATCAACAA TCGCGCCGAA CTGGCCGAGG CCGAAACCAT CTGGCAAAAC CGCAAGCGTC GGGAGCTTAT GCTTTCCGGT GTGACGCTGA TCGCACCTGA AACAGTCTTC TTTTCATATG ACACGGTGAT TGAGCCCGAT GTTGTGATCG AACCGAACGT CTTCTTCGGC CCTTCGGTGC ATGTCGCATC GGGTGCGCTC ATCCATTCCT TCTCGCATCT GGAAGGGGCG CAGGTGGGTG AAAAGGCCGA GATCGGGCCA TTTGCCCGGC TTCGCCCCGG CGCCGATCTG GCGGAAAAAT CCAAGGTCGG CAATTTCTGC GAAGTAAAGA ACGCGAAGGT TGGCAAGGGC GCAAAGATCA ATCACCTGGC CTATATTGGC GATGCGGTCA TCGGCGCTTC CAGCAATATT GGTGCGGGCA CGATCACCTG TAATTATGAT GGCTACAACA AGTTCAAGAC CATCATCGGC GACAATGCCT TTATCGGCTC CAACAGTTCG CTCGTTGCGC CGGTGGAAAT CGGCGACAAC GCCTATATCG CCTCCGGCAG CGTCATCACG GCAGACGTTC CAGCCGATGC GCTGGCCCTT GGCCGCGCCC GTCAGGAGAC GAAGGAAGGC CGGGCAAAGA TCCTGCGGGA GAAGTATGCC GCCATCAAAG CGGCTAAAAG CGTTTCAAAG AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA