SSGCID: Target Page

BrabA.00143.b

SHORT-CHAIN DEHYDROGENASE

CENTER ID:	BrabA.00143.b
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.00143.b.B1.GE37710	Full length( BrabA.00143.b )		1	269

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel86222_1413
BV-BRC:	fig\|359391.4.peg.1413
RefSeq:	YP_414734.1
UniProt:	Q2YQE6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGGQLKGKIA VITGSTQGLG AATARLFAER GAQGLVICGR SAEKGRAQAA GLEELGAKAV FVQADLEKVE DCRRIVAEAD RAFGRLDILV NAAGLTDRGT ILDTSPELFD RLFAVNTRAP FFLIQEAIKL FRHDRVEGAI VNVSSMSSMG GQPFIAAYCA SKGALDTLTR NVAYSVLRNR IRVNSLNIGW MASDGEDRIQ REYHGAPANW LEEAVKTQPF GRLLDPAEVA RAIAFLASEE SGLMTGAIVN FDQSVWGAYD GSPHPEKPL

NT Sequence

ATGGGCGGAC AGTTGAAGGG TAAGATTGCA GTCATTACAG GCAGCACGCA GGGGCTGGGC GCGGCCACCG CCCGGCTGTT TGCCGAACGG GGCGCGCAAG GCCTTGTGAT CTGCGGGCGT TCTGCCGAAA AGGGCAGGGC GCAGGCTGCC GGGCTTGAAG AACTGGGCGC AAAAGCGGTT TTCGTGCAGG CGGACCTTGA AAAGGTGGAA GACTGCCGCC GCATCGTTGC AGAAGCTGAC CGGGCATTCG GCAGGCTTGA TATTCTGGTG AATGCGGCGG GACTGACCGA TCGCGGCACT ATTCTGGATA CATCACCGGA ACTGTTCGAC CGGCTCTTTG CCGTGAACAC GCGCGCGCCG TTTTTCCTTA TTCAGGAAGC GATCAAGCTT TTCCGCCATG ACAGGGTGGA GGGTGCGATT GTGAATGTCT CCTCCATGTC GTCCATGGGA GGCCAGCCTT TTATTGCAGC CTATTGCGCC TCCAAGGGCG CGCTCGACAC GCTGACGCGC AATGTTGCCT ATTCGGTTCT TCGCAACCGC ATTCGGGTCA ACAGCCTCAA TATCGGCTGG ATGGCATCCG ATGGCGAGGA TCGTATCCAG CGTGAATATC ATGGTGCACC GGCAAACTGG CTGGAGGAAG CCGTAAAGAC GCAACCTTTC GGGCGGCTTC TCGACCCGGC GGAAGTGGCG CGCGCCATTG CTTTTCTGGC CTCGGAGGAA TCAGGACTGA TGACGGGTGC TATCGTCAAT TTCGACCAGT CGGTCTGGGG CGCTTATGAC GGTTCTCCGC ATCCTGAGAA GCCGCTT

Details for BrabA.00143.b.B1.GE37710

HARVESTED ON:	2/5/2014
SEQUENCED ON:	2/6/2014
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG GQLKGKIAVI TGSTQGLGAA TARLFAERGA QGLVICGRSA EKGRAQAAGL EELGAKAVFV QADLEKVEDC RRIVAEADRA FGRLDILVNA AGLTDRGTIL DTSPELFDRL FAVNTRAPFF LIQEAIKLFR HDRVEGAIVN VSSMSSMGGQ PFIAAYCASK GALDTLTRNV AYSVLRNRIR VNSLNIGWMA SDGEDRIQRE YHGAPANWLE EAVKTQPFGR LLDPAEVARA IAFLASEESG LMTGAIVNFD QSVWGAYDGS PHPESRFSE

Validated NT Sequence

annncnctag atattttgtt taactttaag aaggagatat accatggctc accaccacca ccaccatatg ggcggacagt tgaagggtaa gattgcagtc attacaggca gcacgcaggg gctgggcgcg gccaccgccc ggctgtttgc cgaacggggc gcgcaaggcc ttgtgatctg cgggcgttct gccgaaaagg gcagggcgca ggctgccggg cttgaagaac tgggcgcaaa agcggttttc gtgcaggcgg accttgaaaa ggtggaagac tgccgccgca tcgttgcaga agctgaccgg gcattcggca ggcttgatat tctggtgaat gcggcgggac tgaccgatcg cggcactatt ctggatacat caccggaact gttcgaccgg ctctttgccg tgaacacgcg cgcgccgttt ttccttattc aggaagcgat caagcttttc cgccatgaca gggtggaggg tgcgattgtg aatgtctcct ccatgtcgtc catgggaggc cagcctttta ttgcagccta ttgcgcctcc aagggcgcgc tcgacacgct gacgcgcaat gttgcctatt cggttcttcg caaccgcatt cgggtcaaca gcctcaatat cggctggatg gcatccgatg gcgaggatcg tatccagcgt gaatatcatg gtgcaccggc aaactggctg gaggaagccg taaagacgca acctttcggg cggcttctcg acccggcgga agtggcgcgc gccattgctt ttctggcctc ggaggaatca ggactgatga cgggtgctat cgtcaatttc gaccagtcgg tctggggcgc ttatgacggt tctccgcatc ctgagagccg ctttagtgag tagatnnatc cnntnnnnnn ncncnnnnnn nnnnnnannn nnacaaannn nantgcgtct ttacngcttc tncancnngt ntcggtgcac ctggattctc ancctggaaa cgatnctcgc catcngatgc catcccgccc ntcnnnnagc ngnnatccnn nnngcngnaa gnanccgana ggnnnnntgc tngtcagcnn gttcagagng cctgnagncg cannagctg

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggcg gacagttgaa gggtaagatt gcagtcatta caggcagcac gcaggggctg ggcgcggcca ccgcccggct gtttgccgaa cggggcgcgc aaggccttgt gatctgcggg cgttctgccg aaaagggcag ggcgcaggct gccgggcttg aagaactggg cgcaaaagcg gttttcgtgc aggcggacct tgaaaaggtg gaagactgcc gccgcatcgt tgcagaagct gaccgggcat tcggcaggct tgatattctg gtgaatgcgg cgggactgac cgatcgcggc actattctgg atacatcacc ggaactgttc gaccggctct ttgccgtgaa cacgcgcgcg ccgtttttcc ttattcagga agcgatcaag cttttccgcc atgacagggt ggagggtgcg attgtgaatg tctcctccat gtcgtccatg ggaggccagc cttttattgc agcctattgc gcctccaagg gcgcgctcga cacgctgacg cgcaatgttg cctattcggt tcttcgcaac cgcattcggg tcaacagcct caatatcggc tggatggcat ccgatggcga ggatcgtatc cagcgtgaat atcatggtgc accggcaaac tggctggagg aagccgtaaa gacgcaacct ttcgggcggc ttctcgaccc ggcggaagtg gcgcgcgcca ttgcttttct ggcctcggag gaatcaggac tgatgacggg tgctatcgtc aatttcgacc agtcggtctg gggcgcttat gacggttctc cgcatcctga gaagccgctt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat