BrabA.00143.a

3-ketoacyl-(acyl-carrier-protein) reductase

CENTER ID: BrabA.00143.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00143.a.A1.PW25615 Full length( BrabA.00143.a ) 1 249

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.2758
RefSeq: YP_418655.1
UniProt: Q2YL29

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNRLQDKVAI ITGAGSGFGL GMARRFAAEG ARVVLADVNV KRVEDGLADI SSNGISIQAD VSRKDDMERV ARTAFEEFGR IDIMVNNAGF THRNMPLTDV DEHNFDLIVA VNMKALYYST QLVVPIMEHQ GGGVIINTAS ATGLHPRKGL TWYCASKGWV VSATKAMALE LAERNIRVNC LCPAESDGEG LKMFLAEDTP EARAALKSTI PLGRFSTPKD VAEAALWLAS DDASMVTGVA LGIDGGYSI
NT Sequence
ATGAATAGGC TGCAAGATAA GGTCGCGATT ATCACCGGCG CCGGTTCCGG CTTCGGTCTG GGCATGGCGC GCCGGTTTGC AGCCGAGGGT GCCAGGGTGG TTCTGGCCGA TGTGAATGTG AAGCGCGTCG AGGACGGGCT TGCCGATATC AGCAGTAACG GCATTTCCAT CCAGGCGGAT GTTTCGCGCA AGGATGACAT GGAGCGTGTG GCGCGCACTG CTTTTGAAGA ATTCGGCCGT ATCGACATTA TGGTCAACAA TGCTGGTTTC ACGCACCGCA ACATGCCGCT GACCGATGTG GACGAGCATA ATTTCGATCT CATCGTCGCG GTGAACATGA AGGCGCTTTA CTATTCGACG CAGCTCGTCG TGCCGATCAT GGAGCATCAG GGCGGCGGTG TTATCATCAA TACGGCGTCT GCCACGGGGC TGCATCCGCG CAAGGGTCTC ACCTGGTATT GCGCGTCCAA GGGCTGGGTG GTGAGCGCCA CGAAAGCCAT GGCGCTGGAG CTTGCGGAGC GGAACATTCG TGTGAATTGC CTGTGCCCGG CGGAAAGCGA CGGTGAGGGA TTGAAGATGT TTCTGGCGGA AGACACGCCG GAAGCGCGCG CAGCACTGAA AAGCACCATT CCGCTTGGCC GGTTTTCCAC GCCGAAGGAT GTTGCGGAAG CCGCGCTCTG GCTGGCCTCC GACGATGCTT CCATGGTGAC GGGCGTGGCG CTCGGTATCG ATGGCGGCTA TTCGATC
Details for BrabA.00143.a.A1.PW25615
PURIFICATION DATe: 6/21/2010
CONCENTRATION: 29.74mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 100µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNRLQDKVA IITGAGSGFG LGMARRFAAE GARVVLADVN VKRVEDGLAD ISSNGISIQA DVSRKDDMER VARTAFEEFG RIDIMVYNAG FTHRNMPLTD VDEHNFDLIV AVNMKALYYS TQLVVPIMEH QGGGVIINTA SATGLHPRKG LTWYCASKGW VVSATKAMAL ELAERNIRVN CLCPAESDGE GLKMFLAEDT PEARAALKST IPLGRFSTPX DVAEAALWLX SDDASMVTGV ALGIDGGYSI
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaata ggctgcaaga taaggtcgcg attatcaccg gcgccggttc cggcttcggt ctgggcatgg cgcgccggtt tgcagccgag ggtgccaggg tggttctggc cgatgtgaat gtgaagcgcg tcgaggacgg gcttgccgat atcagcagta acggcatttc catccaggcg gatgtttcgc gcaaggatga catggagcgt gtggcgcgca ctgcttttga agaattcggc cgtatcgaca ttatggtcta caatgctggt ttcacgcacc gcaacatgcc gctgaccgat gtggacgagc ataatttcga tctcatcgtc gcggtgaaca tgaaggcgct ttactattcg acgcagctcg tcgtgccgat catggagcat cagggcggcg gtgttatcat caatacggcg tctgccacgg ggctgcatcc gcgcaagggt ctcacctggt attgcgcgtc caagggctgg gtggtgagcg ccacgaaagc catggcgctg gagcttgcgg agcggaacat tcgtgtgaat tgcctgtgcc cggcggaaag cgacggtgag ggattgaaga tgtttctggc ggaagacacg ccggaagcgc gcgcagcact gaaaagcacc attccgcttg gccggttttc cacgccgaan gatgttgcgg aagccgcgct ctggctggnc tccgacgatg cttccatggt gacgggcgtg gcgctcggta tcgatggcgg ctattcgatc taataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNRLQDKVA IITGAGSGFG LGMARRFAAE GARVVLADVN VKRVEDGLAD ISSNGISIQA DVSRKDDMER VARTAFEEFG RIDIMVNNAG FTHRNMPLTD VDEHNFDLIV AVNMKALYYS TQLVVPIMEH QGGGVIINTA SATGLHPRKG LTWYCASKGW VVSATKAMAL ELAERNIRVN CLCPAESDGE GLKMFLAEDT PEARAALKST IPLGRFSTPK DVAEAALWLA SDDASMVTGV ALGIDGGYSI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAATAGGCTG CAAGATAAGG TCGCGATTAT CACCGGCGCC GGTTCCGGCT TCGGTCTGGG CATGGCGCGC CGGTTTGCAG CCGAGGGTGC CAGGGTGGTT CTGGCCGATG TGAATGTGAA GCGCGTCGAG GACGGGCTTG CCGATATCAG CAGTAACGGC ATTTCCATCC AGGCGGATGT TTCGCGCAAG GATGACATGG AGCGTGTGGC GCGCACTGCT TTTGAAGAAT TCGGCCGTAT CGACATTATG GTCAACAATG CTGGTTTCAC GCACCGCAAC ATGCCGCTGA CCGATGTGGA CGAGCATAAT TTCGATCTCA TCGTCGCGGT GAACATGAAG GCGCTTTACT ATTCGACGCA GCTCGTCGTG CCGATCATGG AGCATCAGGG CGGCGGTGTT ATCATCAATA CGGCGTCTGC CACGGGGCTG CATCCGCGCA AGGGTCTCAC CTGGTATTGC GCGTCCAAGG GCTGGGTGGT GAGCGCCACG AAAGCCATGG CGCTGGAGCT TGCGGAGCGG AACATTCGTG TGAATTGCCT GTGCCCGGCG GAAAGCGACG GTGAGGGATT GAAGATGTTT CTGGCGGAAG ACACGCCGGA AGCGCGCGCA GCACTGAAAA GCACCATTCC GCTTGGCCGG TTTTCCACGC CGAAGGATGT TGCGGAAGCC GCGCTCTGGC TGGCCTCCGA CGATGCTTCC ATGGTGACGG GCGTGGCGCT CGGTATCGAT GGCGGCTATT CGATCAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA