BrabA.00138.a

Alanyl-transfer RNA synthetase

CENTER ID:	BrabA.00138.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.00138.a.A1.PW25613	Full length( BrabA.00138.a )		1	247

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_132880
PATRIC ID:	fig\|359391.4.peg.1118
RefSeq:	YP_414477.1
UniProt:	Q2YQ19

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAYETEALFR EDSYLSTAEG TVLAIAGNGG IILDRTNFYA TSGGQPGDTG FFERADGSRI EIATTVTGET KNEIVHMPAQ GQQMPDVGEK LVLHIDWQRR YKLMRMHTAC HLLSVACPFP ITGAAVGEDE SRVDFDLPDQ SYTKESVTEK LMELVQANAP VSIHWISDEE FLANPDIVKS KNVRPPVGLG RIRLVAIGEN GSVDSQPCGG THVSETQEVG EIHIGKIEKK GKENRRFRIR FGSLPQA

NT Sequence

ATGGCATACG AGACTGAGGC GCTTTTTCGC GAAGATTCCT ATCTTTCAAC TGCGGAGGGC ACAGTTCTCG CCATCGCCGG AAATGGCGGC ATTATCCTGG ACAGGACGAA TTTCTATGCT ACTTCCGGCG GCCAGCCGGG GGATACCGGT TTCTTCGAGC GCGCGGATGG TTCGCGGATC GAGATCGCGA CGACCGTTAC AGGCGAAACC AAGAATGAAA TTGTCCATAT GCCCGCGCAA GGCCAGCAAA TGCCGGACGT GGGCGAAAAA CTCGTCCTGC ATATAGATTG GCAGCGCCGC TACAAACTGA TGCGTATGCA CACGGCCTGC CATCTGCTTT CCGTCGCCTG CCCGTTCCCG ATCACAGGTG CTGCGGTGGG CGAGGATGAA AGCCGTGTCG ATTTCGACCT GCCGGATCAA AGCTATACGA AGGAATCCGT CACCGAAAAA CTGATGGAAC TGGTGCAGGC CAACGCCCCT GTGTCGATCC ACTGGATCAG CGACGAGGAA TTTCTGGCCA ATCCCGACAT TGTGAAATCG AAAAATGTTC GCCCGCCCGT TGGCCTTGGC CGTATCCGGC TGGTCGCCAT TGGTGAAAAT GGCTCTGTGG ATTCGCAACC TTGCGGCGGC ACACACGTTA GTGAAACACA GGAAGTCGGT GAAATCCATA TTGGCAAGAT CGAAAAGAAG GGCAAGGAAA ACCGCCGTTT CCGCATTCGC TTCGGCAGCT TGCCGCAAGC C

Details for BrabA.00138.a.A1.PW25613

PURIFICATION DATe:	4/5/2010
CONCENTRATION:	23.16mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAYETEALF REDSYLSTAE GTVLAIAGNG GIILDRTNFY ATSGGQPGDT GFFERADGSR IEIATTVTGE TKNEIVHMPA QGQQMPDVGE KLVLHIDWQR RYKLMRMHTA CHLLSVACPF PITGAAVGED ESRVDFDLPD QSYTKESVTE KLMELVQANA PVSIHWISDE EFLANPDIVK SKNVRPPVGL GRIRLVAIGE NGSVDSQPCG GTHVSETQEV GEIHIGKIEK KGKENRRFRI RFGSL

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcat acgagactga ggcgcttttt cgcgaagatt cctatctttc aactgcggag ggcacagttc tcgccatcgc cggaaatggc ggcattatcc tggacaggac gaatttctat gctacttccg gcggccagcc gggggatacc ggtttcttcg agcgcgcgga tggttcgcgg atcgagatcg cgacgaccgt tacaggcgaa accaagaatg aaattgtcca tatgcccgcg caaggccagc aaatgccgga cgtgggcgaa aaactcgtcc tgcatataga ttggcagcgc cgctacaaac tgatgcgtat gcacacggcc tgccatctgc tttccgtcgc ctgcccgttc ccgatcacag gtgctgcggt gggcgaggat gaaagccgtg tcgatttcga cctgccggat caaagctata cgaaggaatc cgtcaccgaa aaactgatgg aactggtgca ggccaacgcc cctgtgtcga tccactggat cagcgacgag gaatttctgg ccaatcccga cattgtgaaa tcgaaaaatg ttcgcccgcc cgttggcctt ggccgtatcc ggctggtcgc cattggtgaa aatggctctg tggattcgca accttgcggc ggcacacacg ttagtgaaac acaggaagtc ggtgaaatcc atattggcaa gatcgaaaag aagggcaagg aaaaccgccg tttccgcatt cgcttcggca gcttgccg

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCATACGAG ACTGAGGCGC TTTTTCGCGA AGATTCCTAT CTTTCAACTG CGGAGGGCAC AGTTCTCGCC ATCGCCGGAA ATGGCGGCAT TATCCTGGAC AGGACGAATT TCTATGCTAC TTCCGGCGGC CAGCCGGGGG ATACCGGTTT CTTCGAGCGC GCGGATGGTT CGCGGATCGA GATCGCGACG ACCGTTACAG GCGAAACCAA GAATGAAATT GTCCATATGC CCGCGCAAGG CCAGCAAATG CCGGACGTGG GCGAAAAACT CGTCCTGCAT ATAGATTGGC AGCGCCGCTA CAAACTGATG CGTATGCACA CGGCCTGCCA TCTGCTTTCC GTCGCCTGCC CGTTCCCGAT CACAGGTGCT GCGGTGGGCG AGGATGAAAG CCGTGTCGAT TTCGACCTGC CGGATCAAAG CTATACGAAG GAATCCGTCA CCGAAAAACT GATGGAACTG GTGCAGGCCA ACGCCCCTGT GTCGATCCAC TGGATCAGCG ACGAGGAATT TCTGGCCAAT CCCGACATTG TGAAATCGAA AAATGTTCGC CCGCCCGTTG GCCTTGGCCG TATCCGGCTG GTCGCCATTG GTGAAAATGG CTCTGTGGAT TCGCAACCTT GCGGCGGCAC ACACGTTAGT GAAACACAGG AAGTCGGTGA AATCCATATT GGCAAGATCG AAAAGAAGGG CAAGGAAAAC CGCCGTTTCC GCATTCGCTT CGGCAGCTTG CCGCAAGCCA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A