BrabA.00133.a

cysteinyl-tRNA synthetase

CENTER ID:	BrabA.00133.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.00133.a.A1.GE25567	Full length( BrabA.00133.a )		1	506

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.00133.a.A1.PW25612	Full length( BrabA.00133.a )		1	506

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126755
PATRIC ID:	fig\|359391.4.peg.726
RefSeq:	YP_414139.1
UniProt:	Q2YN22

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPDTTPQLRL YNTLTRTKEA FAPIDAKNVR MYVCGPTVYD FAHIGNARPV IVFDVLFRLL RHVYGAQHVT YARNITDVDD KINARAARDY PDLPFNEAIR KVTESTNAQF QADVTALGNL QPTVQPRATE HMDEMRAMID RLVQRGVAYV AQDHVLFSPS AMNARKGPRY GALARRSLDE MLAGARVDVA SYKRDEMDFV LWKPSKKGEP GWPSPAGIET LGRPGWHIEC SAMSMAKLLE PFGGGLKCDD PERNQFDIHG GGIDLVFPHH ENEIAQSCCA LGTERMANIW MHNGFLQVEG QKMSKSLGNF ITIRDVLNDG LPQLGEWGDN TVRDHWAGLA ARLSMLQTHY REPINWTAQR LAESADELHR WYGLLRDEGF GAPEKLSHAS AVAAALCDDL NSWAAITALR QAFKVRDVAA LGEGMALMGL LDPYFVTASD VPIFARADVD ASAIAARIAE RLNFINAKNW AEADRIRDEL LQEGVQLKDS KDPATGERIT TWDVVG

NT Sequence

ATGCCGGATA CCACGCCGCA ATTGCGTCTT TATAATACGC TGACCCGCAC GAAGGAGGCA TTTGCCCCCA TCGATGCGAA GAACGTGCGC ATGTATGTCT GCGGCCCGAC GGTTTATGAT TTTGCGCATA TTGGCAATGC GCGCCCGGTG ATCGTGTTCG ACGTGCTGTT CCGTCTGTTG CGCCATGTTT ACGGCGCGCA GCATGTCACC TATGCGCGCA ACATTACCGA TGTGGATGAC AAGATCAATG CGCGCGCCGC GCGTGATTAT CCCGATCTTC CCTTCAATGA AGCGATCCGC AAAGTCACCG AAAGCACCAA TGCGCAGTTT CAGGCCGATG TGACCGCGCT TGGTAATCTT CAGCCCACGG TCCAGCCGCG CGCAACCGAA CATATGGACG AAATGCGCGC CATGATCGAC CGGCTGGTGC AGCGCGGCGT GGCCTATGTG GCGCAAGATC ATGTGCTCTT CTCGCCTTCG GCCATGAATG CGCGCAAGGG GCCGCGCTAT GGCGCGCTGG CCCGCCGCTC GCTCGATGAA ATGCTGGCAG GCGCGCGCGT CGATGTCGCG TCCTACAAGC GCGACGAGAT GGATTTCGTG CTCTGGAAAC CCTCGAAGAA AGGTGAACCG GGCTGGCCCT CACCGGCTGG TATTGAAACT CTGGGCCGTC CGGGCTGGCA TATCGAGTGC TCGGCCATGT CGATGGCCAA GCTTCTGGAG CCGTTCGGCG GCGGGTTGAA ATGCGACGAC CCGGAAAGGA ACCAGTTCGA CATTCATGGC GGCGGTATCG ATCTTGTCTT TCCGCACCAC GAAAACGAGA TTGCCCAGTC CTGCTGCGCA TTGGGAACCG AACGCATGGC CAATATCTGG ATGCATAACG GTTTTCTTCA GGTCGAGGGG CAGAAGATGT CGAAATCGCT CGGCAACTTC ATCACCATCC GCGACGTGCT GAATGATGGC CTGCCGCAAT TGGGCGAGTG GGGCGATAAT ACGGTGCGCG ACCACTGGGC CGGTCTTGCG GCGCGCCTTT CCATGCTTCA GACCCATTAT CGCGAGCCGA TCAACTGGAC GGCGCAGCGT CTGGCGGAAT CGGCTGATGA ATTGCATCGC TGGTATGGCC TACTGCGCGA CGAAGGCTTT GGCGCGCCGG AAAAACTGTC TCACGCCAGT GCGGTTGCTG CGGCCCTTTG CGATGATCTT AATAGCTGGG CGGCAATCAC TGCCTTGCGT CAGGCGTTCA AGGTCAGGGA CGTCGCTGCT CTGGGTGAGG GGATGGCGCT GATGGGCCTG CTTGATCCTT ATTTCGTGAC GGCTTCCGAT GTGCCGATCT TTGCCAGGGC AGATGTGGAT GCGAGCGCCA TTGCGGCGCG GATTGCGGAA CGTCTGAACT TCATCAACGC GAAAAACTGG GCTGAGGCGG ACCGCATTCG CGACGAGCTT CTACAGGAAG GCGTGCAGTT GAAGGATAGC AAGGACCCTG CGACTGGCGA GCGCATAACG ACATGGGATG TGGTAGGG

Details for BrabA.00133.a.A1.GE25567

HARVESTED ON:	9/10/2008
SEQUENCED ON:	7/28/2010
EXPECTED MW:	58kDa
OBSERVED MW:	58kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	71
PERCENT COVERAGE:	43

Validated AA Sequence

MDEMRAMIDR LVQRGVAYVA QDHVLFSPSA MNARKGPRYG ALARRSLDEM LAGARVDVAS YKRDEMDFVL WKPSKKGEPG WPSPAGIETL GRPGWXIXCS AMSMAKLLEP FGGGLKCXDP ERNXFXIHGG GXDLVFPHHE NEIXXXCCAX GTERMXXIWX HNXFLQXXGQ KXXKSLXNFX XXRXVLXXXP AXXXXGXXXX XXXXGXXXXX XXXXHXXXXX XXXXXSXXXX X

Validated NT Sequence

annnnngttn nntttannan nnnagatata ccatggctca tcnccntcnc nntcatntgg gtacccngna agctcaaccc nnanncctgg ttcgatgccg gataccacgc cgcaattgcg tctttataat acgctgaccc gcacgaagga ggcatttgcc cccatcgatg cnaagaacgt gcgcatgtat gtctgcggcc cgacggttta tgattttgcg catattggca atgcgcgccc ggtgatcgtg ttcgacgtgc tgttccgtct gttgcgccat gtttacggcg cgcagcatgt cacctatgcg cgcaacatta ccgatgtgga tgacaagatc aatgcgcgcg ccgcgcgtga ttatcccgat cttcccttca atgaagcgat ccgcaaagtc accgaaagca ccaatgcgca gtttcnnncg atgtgaccgc gcttggtaat cttcagccca cggtccagcc gcgcgcaacc gaacatatgg acgaaatgcg cgccatgatc gaccggctgg tgcagcgcgg cgtggcctat gtggcgcaag atcatgtgct cttctcgcct tcggccatga atgcgcgcaa ggggccgcgc tatggcgcgc tggcccgccg ctcgctcgat gaaatgctgg caggcgcgcg cgtcgatgtc gcgtcctaca agcgcgacga gatggatttc gtgctctgga aaccctcgaa gaaaggtgaa ccgggctggc cctcaccggc tggtattgaa actctgggcc gtccgggctg gnatatcnag tgctcggcca tgtcgatggc caagcttctg gagccgttcg gcggcgggtt gaaatgcnac gacccggaaa ggaaccngtt cnacattcat ggcggcggnn tcgatcttgt ctttccgcac cacgaaaacg agattnncca nnnctgctgc gcntnnggaa ccgaacgcat ggncannatc tggntgcata acgnttttct tcaggncnan gggcagaaga ngnngaaatc gctcnncaac ttcntcnccn tccgcnncgt gctgaangan ngncctgcnn cnnnggnnnn nggggnnann nnnnnngnnc nannnnnggg nnnnnntnnn nnnnnnntnn ttnnnancca ttannnnnnn nnnatnannn nnnnncnnnn ntctnnnnnn annnnnnnnn nt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPDTTPQLR LYNTLTRTKE AFAPIDAKNV RMYVCGPTVY DFAHIGNARP VIVFDVLFRL LRHVYGAQHV TYARNITDVD DKINARAARD YPDLPFNEAI RKVTESTNAQ FQADVTALGN LQPTVQPRAT EHMDEMRAMI DRLVQRGVAY VAQDHVLFSP SAMNARKGPR YGALARRSLD EMLAGARVDV ASYKRDEMDF VLWKPSKKGE PGWPSPAGIE TLGRPGWHIE CSAMSMAKLL EPFGGGLKCD DPERNQFDIH GGGIDLVFPH HENEIAQSCC ALGTERMANI WMHNGFLQVE GQKMSKSLGN FITIRDVLND GLPQLGEWGD NTVRDHWAGL AARLSMLQTH YREPINWTAQ RLAESADELH RWYGLLRDEG FGAPEKLSHA SAVAAALCDD LNSWAAITAL RQAFKVRDVA ALGEGMALMG LLDPYFVTAS DVPIFARADV DASAIAARIA ERLNFINAKN WAEADRIRDE LLQEGVQLKD SKDPATGERI TTWDVVG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccggatacc acgccgcaat tgcgtcttta taatacgctg acccgcacga aggaggcatt tgcccccatc gatgcgaaga acgtgcgcat gtatgtctgc ggcccgacgg tttatgattt tgcgcatatt ggcaatgcgc gcccggtgat cgtgttcgac gtgctgttcc gtctgttgcg ccatgtttac ggcgcgcagc atgtcaccta tgcgcgcaac attaccgatg tggatgacaa gatcaatgcg cgcgccgcgc gtgattatcc cgatcttccc ttcaatgaag cgatccgcaa agtcaccgaa agcaccaatg cgcagtttca ggccgatgtg accgcgcttg gtaatcttca gcccacggtc cagccgcgcg caaccgaaca tatggacgaa atgcgcgcca tgatcgaccg gctggtgcag cgcggcgtgg cctatgtggc gcaagatcat gtgctcttct cgccttcggc catgaatgcg cgcaaggggc cgcgctatgg cgcgctggcc cgccgctcgc tcgatgaaat gctggcaggc gcgcgcgtcg atgtcgcgtc ctacaagcgc gacgagatgg atttcgtgct ctggaaaccc tcgaagaaag gtgaaccggg ctggccctca ccggctggta ttgaaactct gggccgtccg ggctggcata tcgagtgctc ggccatgtcg atggccaagc ttctggagcc gttcggcggc gggttgaaat gcgacgaccc ggaaaggaac cagttcgaca ttcatggcgg cggtatcgat cttgtctttc cgcaccacga aaacgagatt gcccagtcct gctgcgcatt gggaaccgaa cgcatggcca atatctggat gcataacggt tttcttcagg tcgaggggca gaagatgtcg aaatcgctcg gcaacttcat caccatccgc gacgtgctga atgatggcct gccgcaattg ggcgagtggg gcgataatac ggtgcgcgac cactgggccg gtcttgcggc gcgcctttcc atgcttcaga cccattatcg cgagccgatc aactggacgg cgcagcgtct ggcggaatcg gctgatgaat tgcatcgctg gtatggccta ctgcgcgacg aaggctttgg cgcgccggaa aaactgtctc acgccagtgc ggttgctgcg gccctttgcg atgatcttaa tagctgggcg gcaatcactg ccttgcgtca ggcgttcaag gtcagggacg tcgctgctct gggtgagggg atggcgctga tgggcctgct tgatccttat ttcgtgacgg cttccgatgt gccgatcttt gccagggcag atgtggatgc gagcgccatt gcggcgcgga ttgcggaacg tctgaacttc atcaacgcga aaaactgggc tgaggcggac cgcattcgcg acgagcttct acaggaaggc gtgcagttga aggatagcaa ggaccctgcg actggcgagc gcataacgac atgggatgtg gtagggaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for BrabA.00133.a.A1.PW25612

PURIFICATION DATe:	9/22/2008
CONCENTRATION:	25.62mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	36
VIAL VOLUME:	100µl
PERCENT IDENTITY:	71
PERCENT COVERAGE:	43

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCGGATACC ACGCCGCAAT TGCGTCTTTA TAATACGCTG ACCCGCACGA AGGAGGCATT TGCCCCCATC GATGCGAAGA ACGTGCGCAT GTATGTCTGC GGCCCGACGG TTTATGATTT TGCGCATATT GGCAATGCGC GCCCGGTGAT CGTGTTCGAC GTGCTGTTCC GTCTGTTGCG CCATGTTTAC GGCGCGCAGC ATGTCACCTA TGCGCGCAAC ATTACCGATG TGGATGACAA GATCAATGCG CGCGCCGCGC GTGATTATCC CGATCTTCCC TTCAATGAAG CGATCCGCAA AGTCACCGAA AGCACCAATG CGCAGTTTCA GGCCGATGTG ACCGCGCTTG GTAATCTTCA GCCCACGGTC CAGCCGCGCG CAACCGAACA TATGGACGAA ATGCGCGCCA TGATCGACCG GCTGGTGCAG CGCGGCGTGG CCTATGTGGC GCAAGATCAT GTGCTCTTCT CGCCTTCGGC CATGAATGCG CGCAAGGGGC CGCGCTATGG CGCGCTGGCC CGCCGCTCGC TCGATGAAAT GCTGGCAGGC GCGCGCGTCG ATGTCGCGTC CTACAAGCGC GACGAGATGG ATTTCGTGCT CTGGAAACCC TCGAAGAAAG GTGAACCGGG CTGGCCCTCA CCGGCTGGTA TTGAAACTCT GGGCCGTCCG GGCTGGCATA TCGAGTGCTC GGCCATGTCG ATGGCCAAGC TTCTGGAGCC GTTCGGCGGC GGGTTGAAAT GCGACGACCC GGAAAGGAAC CAGTTCGACA TTCATGGCGG CGGTATCGAT CTTGTCTTTC CGCACCACGA AAACGAGATT GCCCAGTCCT GCTGCGCATT GGGAACCGAA CGCATGGCCA ATATCTGGAT GCATAACGGT TTTCTTCAGG TCGAGGGGCA GAAGATGTCG AAATCGCTCG GCAACTTCAT CACCATCCGC GACGTGCTGA ATGATGGCCT GCCGCAATTG GGCGAGTGGG GCGATAATAC GGTGCGCGAC CACTGGGCCG GTCTTGCGGC GCGCCTTTCC ATGCTTCAGA CCCATTATCG CGAGCCGATC AACTGGACGG CGCAGCGTCT GGCGGAATCG GCTGATGAAT TGCATCGCTG GTATGGCCTA CTGCGCGACG AAGGCTTTGG CGCGCCGGAA AAACTGTCTC ACGCCAGTGC GGTTGCTGCG GCCCTTTGCG ATGATCTTAA TAGCTGGGCG GCAATCACTG CCTTGCGTCA GGCGTTCAAG GTCAGGGACG TCGCTGCTCT GGGTGAGGGG ATGGCGCTGA TGGGCCTGCT TGATCCTTAT TTCGTGACGG CTTCCGATGT GCCGATCTTT GCCAGGGCAG ATGTGGATGC GAGCGCCATT GCGGCGCGGA TTGCGGAACG TCTGAACTTC ATCAACGCGA AAAACTGGGC TGAGGCGGAC CGCATTCGCG ACGAGCTTCT ACAGGAAGGC GTGCAGTTGA AGGATAGCAA GGACCCTGCG ACTGGCGAGC GCATAACGAC ATGGGATGTG GTAGGGAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA