BrabA.00128.a

Bacterial extracellular solute-binding protein, family 3:Pyridoxal-dependent decarboxylase

CENTER ID: BrabA.00128.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.00128.a.A2.GE28075 Created from Decode construct for feature BrabA.00128.a 1 288
BrabA.00128.a.A4.GE28077 Created from Decode construct for feature BrabA.00128.a 35 304
BrabA.00128.a.A5.GE28078 Created from Decode construct for feature BrabA.00128.a 35 288
BrabA.00128.a.A6.GE28079 Created from Decode construct for feature BrabA.00128.a 35 292
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_129592
PATRIC ID: fig|359391.4.peg.3158
RefSeq: YP_419019
UniProt: Q2YK15
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MCWHKFARYW DVEIREIPME PGRLFMGPEQ MLEAVDENTI GVVPTFGVTY TGNYEFPEPL QDALDKLQKT KGLDIDIHVD AASGGFLAPF CAPDIPWDFR LPRVKSISAS GHKYGLAPLG CGWVVWRDKE ALPEELIFNV DYLGGQVGTF AINFSRPAGQ VISQYYEFMR LGREGYTKVQ QAAYRVAQYI AREIEPLGPY EFICAGEEKG GIPAVCFRIR EGEDPGYSLY DLSERLRLTG WQVPAFALSG KASDITVMRV MCRCGFEMDL AALFIRDFKA GIEFFKSHPS PKITPSMGTG FHHT
NT Sequence
GTGTGTTGGC ACAAATTCGC CCGGTACTGG GATGTCGAGA TCCGGGAAAT TCCGATGGAG CCTGGCCGCC TGTTCATGGG ACCAGAACAA ATGCTTGAAG CCGTTGATGA AAATACCATC GGCGTCGTGC CTACTTTCGG CGTGACCTAT ACCGGCAATT ATGAATTTCC CGAACCGTTG CAGGATGCGC TGGACAAGCT GCAAAAGACC AAGGGTCTTG ATATCGATAT CCATGTCGAT GCGGCAAGCG GCGGCTTTCT GGCACCATTT TGCGCGCCCG ATATTCCGTG GGATTTCCGC CTGCCGCGCG TGAAGTCGAT CAGCGCGTCA GGCCATAAAT ACGGTCTCGC ACCGCTTGGC TGTGGCTGGG TTGTCTGGCG CGACAAAGAG GCCCTGCCGG AAGAGCTGAT CTTCAATGTG GATTATCTCG GTGGACAGGT TGGCACTTTC GCAATCAATT TCTCGCGGCC TGCCGGTCAG GTCATTTCAC AATATTACGA ATTCATGCGT CTTGGCCGCG AAGGCTACAC CAAGGTGCAG CAGGCGGCCT ACCGCGTGGC GCAATATATA GCCAGGGAGA TCGAGCCGCT TGGGCCTTAT GAATTCATCT GCGCGGGCGA GGAGAAAGGC GGCATTCCCG CCGTCTGTTT CCGCATCCGT GAAGGCGAAG ACCCCGGTTA TTCGCTCTAT GATCTGTCGG AACGGCTGCG TCTGACCGGA TGGCAGGTTC CCGCTTTCGC TCTTTCGGGC AAGGCGTCGG ATATCACTGT CATGCGCGTC ATGTGCCGCT GCGGTTTCGA GATGGATCTG GCTGCACTCT TCATTCGGGA TTTCAAGGCT GGGATCGAGT TCTTCAAATC GCATCCTTCA CCGAAGATTA CCCCGTCGAT GGGAACCGGC TTTCACCACA CA
Details for BrabA.00128.a.A2.GE28075
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVCWHKFAR YWDVEIREIP MEPGRLFMGP EQMLEAVDEN TIGVVPTFGV TYTGNYEFPE PLQDALDKLQ KTKGLDIDIH VDAASGGFLA PFCAPDIPWD FRLPRVKSIS ASGHKYGLAP LGCGWVVWRD KEALPEELIF NVDYLGGQVG TFAINFSRPA GQVISQYYEF MRLGREGYTK VQQAAYRVAQ YIAREIEPLG PYEFICAGEE KGGIPAVCFR IREGEDPGYS LYDLSERLRL TGWQVPAFAL SGKASDITVM RVMCRCGFEM DLAALFIRDF KAGIEFFKSH
Validated NT Sequence
aaggagatat accatggctc atcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatgg tgtgttggca caaattcgcc cggtactggg atgtcgagat ccgggaaatt ccgatggagc ctggccgcct gttcatggga ccagaacaaa tgcttgaagc cgttgatgaa aataccatcg gcgtcgtgcc tactttcggc gtgacctata ccggcaatta tgaatttccc gaaccgttgc aggatgcgct ggacaagctg caaaagacca agggtcttga tatcgatatc catgtcgatg cggcaagcgg cggctttctg gcaccatttt gcgcgcccga tattccgtgg gatttccgcc tgccgcgcgt gaagtcgatc agcgcgtcag gccataaata cggtctcgca ccgcttggct gtggctgggt tgtctggcgc gacaaagagg ccctgccgga agagctgatc ttcaatgtgg attatctcgg tggacaggtt ggcactttcg caatcaattt ctcgcggcct gccggtcagg tcatttcaca atattacgaa ttcatgcgtc ttggccgcga aggctacacc aaggtgcagc aggcggccta ccgcgtggcg caatatatag ccagggagat cgagccgctt gggccttatg aattcatctg cgcgggcgag gagaaaggcg gcattcccgc cgtctgtttc cgcatccgtg aaggcgaaga ccccggttat tcgctctatg atctgtcgga acggctgcgt ctgaccggat ggcaggttcc cgctttcgct ctttcgggca aggcgtcgga tatcactgtc atgcgcgtca tgtgccgctg cggtttcgag atggatctgg ctgcactctt cattcgggat ttcaaggctg ggatcgagtt cttcaaatcg cattaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVCWHKFAR YWDVEIREIP MEPGRLFMGP EQMLEAVDEN TIGVVPTFGV TYTGNYEFPE PLQDALDKLQ KTKGLDIDIH VDAASGGFLA PFCAPDIPWD FRLPRVKSIS ASGHKYGLAP LGCGWVVWRD KEALPEELIF NVDYLGGQVG TFAINFSRPA GQVISQYYEF MRLGREGYTK VQQAAYRVAQ YIAREIEPLG PYEFICAGEE KGGIPAVCFR IREGEDPGYS LYDLSERLRL TGWQVPAFAL SGKASDITVM RVMCRCGFEM DLAALFIRDF KAGIEFFKSH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtgttgg cacaaattcg cccggtactg ggatgtcgag atccgggaaa ttccgatgga gcctggccgc ctgttcatgg gaccagaaca aatgcttgaa gccgttgatg aaaataccat cggcgtcgtg cctactttcg gcgtgaccta taccggcaat tatgaatttc ccgaaccgtt gcaggatgcg ctggacaagc tgcaaaagac caagggtctt gatatcgata tccatgtcga tgcggcaagc ggcggctttc tggcaccatt ttgcgcgccc gatattccgt gggatttccg cctgccgcgc gtgaagtcga tcagcgcgtc aggccataaa tacggtctcg caccgcttgg ctgtggctgg gttgtctggc gcgacaaaga ggccctgccg gaagagctga tcttcaatgt ggattatctc ggtggacagg ttggcacttt cgcaatcaat ttctcgcggc ctgccggtca ggtcatttca caatattacg aattcatgcg tcttggccgc gaaggctaca ccaaggtgca gcaggcggcc taccgcgtgg cgcaatatat agccagggag atcgagccgc ttgggcctta tgaattcatc tgcgcgggcg aggagaaagg cggcattccc gccgtctgtt tccgcatccg tgaaggcgaa gaccccggtt attcgctcta tgatctgtcg gaacggctgc gtctgaccgg atggcaggtt cccgctttcg ctctttcggg caaggcgtcg gatatcactg tcatgcgcgt catgtgccgc tgcggtttcg agatggatct ggctgcactc ttcattcggg atttcaaggc tgggatcgag ttcttcaaat cgcataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BrabA.00128.a.A4.GE28077
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVDENTIGV VPTFGVTYTG NYEFPEPLQD ALDKLQKTKG LDIDIHVDAA SGGFLAPFCA PDIPWDFRLP RVKSISASGH KYGLAPLGCG WVVWRDKEAL PEELIFNVDY LGGQVGTFAI NFSRPAGQVI SQYYEFMRLG REGYTKVQQA AYRVAQYIAR EIEPLGPYEF ICAGEEKGGI PAVCFRIREG EDPGYSLYDL SERLRLTGWQ VPAFALSGKA SDITVMRVMC RCGFEMDLAA LFIRDFKAGI EFFKSHPSPK ITPSMGTG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggttg atgaaaatac catcggcgtc gtgcctactt tcggcgtgac ctataccggc aattatgaat ttcccgaacc gttgcaggat gcgctggaca agctgcaaaa gaccaagggt cttgatatcg atatccatgt cgatgcggca agcggcggct ttctggcacc attttgcgcg cccgatattc cgtgggattt ccgcctgccg cgcgtgaagt cgatcagcgc gtcaggccat aaatacggtc tcgcaccgct tggctgtggc tgggttgtct ggcgcgacaa agaggccctg ccggaagagc tgatcttcaa tgtggattat ctcggtggac aggttggcac tttcgcaatc aatttctcgc ggcctgccgg tcaggtcatt tcacaatatt acgaattcat gcgtcttggc cgcgaaggct acaccaaggt gcagcaggcg gcctaccgcg tggcgcaata tatagccagg gagatcgagc cgcttgggcc ttatgaattc atctgcgcgg gcgaggagaa aggcggcatt cccgccgtct gtttccgcat ccgtgaaggc gaagaccccg gttattcgct ctatgatctg tcggaacggc tgcgtctgac cggatggcag gttcccgctt tcgctctttc gggcaaggcg tcggatatca ctgtcatgcg cgtcatgtgc cgctgcggtt tcgagatgga tctggctgca ctcttcattc gggatttcaa ggctgggatc gagttcttca aatcgcatcc ttcaccgaag attaccccgt cgatgggaac cggcttt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVDENTIGV VPTFGVTYTG NYEFPEPLQD ALDKLQKTKG LDIDIHVDAA SGGFLAPFCA PDIPWDFRLP RVKSISASGH KYGLAPLGCG WVVWRDKEAL PEELIFNVDY LGGQVGTFAI NFSRPAGQVI SQYYEFMRLG REGYTKVQQA AYRVAQYIAR EIEPLGPYEF ICAGEEKGGI PAVCFRIREG EDPGYSLYDL SERLRLTGWQ VPAFALSGKA SDITVMRVMC RCGFEMDLAA LFIRDFKAGI EFFKSHPSPK ITPSMGTGFH HT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggttgatgaa aataccatcg gcgtcgtgcc tactttcggc gtgacctata ccggcaatta tgaatttccc gaaccgttgc aggatgcgct ggacaagctg caaaagacca agggtcttga tatcgatatc catgtcgatg cggcaagcgg cggctttctg gcaccatttt gcgcgcccga tattccgtgg gatttccgcc tgccgcgcgt gaagtcgatc agcgcgtcag gccataaata cggtctcgca ccgcttggct gtggctgggt tgtctggcgc gacaaagagg ccctgccgga agagctgatc ttcaatgtgg attatctcgg tggacaggtt ggcactttcg caatcaattt ctcgcggcct gccggtcagg tcatttcaca atattacgaa ttcatgcgtc ttggccgcga aggctacacc aaggtgcagc aggcggccta ccgcgtggcg caatatatag ccagggagat cgagccgctt gggccttatg aattcatctg cgcgggcgag gagaaaggcg gcattcccgc cgtctgtttc cgcatccgtg aaggcgaaga ccccggttat tcgctctatg atctgtcgga acggctgcgt ctgaccggat ggcaggttcc cgctttcgct ctttcgggca aggcgtcgga tatcactgtc atgcgcgtca tgtgccgctg cggtttcgag atggatctgg ctgcactctt cattcgggat ttcaaggctg ggatcgagtt cttcaaatcg catccttcac cgaagattac cccgtcgatg ggaaccggct ttcaccacac aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BrabA.00128.a.A5.GE28078
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVDENTIGV VPTFGVTYTG NYEFPEPLQD ALDKLQKTKG LDIDIHVDAA SGGFLAPFCA PDIPWDFRLP RVKSISASGH KYGLAPLGCG WVVWRDKEAL PEELIFNVDY LGGQVGTFAI NFSRPAGQVI SQYYEFMRLG REGYTKVQQA AYRVAQYIAR EIEPLGPYEF ICAGEEKGGI PAVCFRIREG EDPGYSLYDL SERLRLTGWQ VPAFALSGKA SDITVMRVMC RCGFEMDLAA LFIRDFKAGI EFFKSH
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggttg atgaaaatac catcggcgtc gtgcctactt tcggcgtgac ctataccggc aattatgaat ttcccgaacc gttgcaggat gcgctggaca agctgcaaaa gaccaagggt cttgatatcg atatccatgt cgatgcggca agcggcggct ttctggcacc attttgcgcg cccgatattc cgtgggattt ccgcctgccg cgcgtgaagt cgatcagcgc gtcaggccat aaatacggtc tcgcaccgct tggctgtggc tgggttgtct ggcgcgacaa agaggccctg ccggaagagc tgatcttcaa tgtggattat ctcggtggac aggttggcac tttcgcaatc aatttctcgc ggcctgccgg tcaggtcatt tcacaatatt acgaattcat gcgtcttggc cgcgaaggct acaccaaggt gcagcaggcg gcctaccgcg tggcgcaata tatagccagg gagatcgagc cgcttgggcc ttatgaattc atctgcgcgg gcgaggagaa aggcggcatt cccgccgtct gtttccgcat ccgtgaaggc gaagaccccg gttattcgct ctatgatctg tcggaacggc tgcgtctgac cggatggcag gttcccgctt tcgctctttc gggcaaggcg tcggatatca ctgtcatgcg cgtcatgtgc cgctgcggtt tcgagatgga tctggctgca ctcttcattc gggatttcaa ggctgggatc gagttcttca aatcgcatta ataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVDENTIGV VPTFGVTYTG NYEFPEPLQD ALDKLQKTKG LDIDIHVDAA SGGFLAPFCA PDIPWDFRLP RVKSISASGH KYGLAPLGCG WVVWRDKEAL PEELIFNVDY LGGQVGTFAI NFSRPAGQVI SQYYEFMRLG REGYTKVQQA AYRVAQYIAR EIEPLGPYEF ICAGEEKGGI PAVCFRIREG EDPGYSLYDL SERLRLTGWQ VPAFALSGKA SDITVMRVMC RCGFEMDLAA LFIRDFKAGI EFFKSH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggttgatgaa aataccatcg gcgtcgtgcc tactttcggc gtgacctata ccggcaatta tgaatttccc gaaccgttgc aggatgcgct ggacaagctg caaaagacca agggtcttga tatcgatatc catgtcgatg cggcaagcgg cggctttctg gcaccatttt gcgcgcccga tattccgtgg gatttccgcc tgccgcgcgt gaagtcgatc agcgcgtcag gccataaata cggtctcgca ccgcttggct gtggctgggt tgtctggcgc gacaaagagg ccctgccgga agagctgatc ttcaatgtgg attatctcgg tggacaggtt ggcactttcg caatcaattt ctcgcggcct gccggtcagg tcatttcaca atattacgaa ttcatgcgtc ttggccgcga aggctacacc aaggtgcagc aggcggccta ccgcgtggcg caatatatag ccagggagat cgagccgctt gggccttatg aattcatctg cgcgggcgag gagaaaggcg gcattcccgc cgtctgtttc cgcatccgtg aaggcgaaga ccccggttat tcgctctatg atctgtcgga acggctgcgt ctgaccggat ggcaggttcc cgctttcgct ctttcgggca aggcgtcgga tatcactgtc atgcgcgtca tgtgccgctg cggtttcgag atggatctgg ctgcactctt cattcgggat ttcaaggctg ggatcgagtt cttcaaatcg cataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BrabA.00128.a.A6.GE28079
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 31kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVDENTIGV VPTFGVTYTG NYEFPEPLQD ALDKLQKTKG LDIDIHVDAA SGGFLAPFCA PDIPWDFRLP RVKSISASGH KYGLAPLGCG WVVWRDKEAL PEELIFNVDY LGGQVGTFAI NFSRPAGQVI SQYYEFMRLG REGYTKVQQA AYRVAQYIAR EIEPLGPYEF ICAGEEKGGI PAVCFRIREG EDPGYSLYDL SERLRLTGWQ VPAFALSGKA SDITVMRVMC RCGFEMDLAA LFIRDFKAGI EFFKSHPSPK
Validated NT Sequence
ttattacttc ggtgaaggat gcgatttgaa gaactcgatc ccagccttga aatcccgaat gaagagtgca gccagatcca tctcgaaacc gcagcggcac atgacgcgca tgacagtgat atccgacgcc ttgcccgaaa gagcgaaagc gggaacctgc catccggtca gacgcagccg ttccgacaga tcatagagcg aataaccggg gtcttcgcct tcacggatgc ggaaacagac ggcgggaatg ccgcctttct cctcgcccgc gcagatgaat tcataaggcc caagcggctc gatctccctg gctatatatt gcgccacgcg gtaggccgcc tgctgcacct tggtgtagcc ttcgcggcca agacgcatga attcgtaata ttgtgaaatg acctgaccgg caggccgcga gaaattgatt gcgaaagtgc caacctgtcc accgagataa tccacattga agatcagctc ttccggcagg gcctctttgt cgcgccagac aacccagcca cagccaagcg gtgcgagacc gtatttatgg cctgacgcgc tgatcgactt cacgcgcggc aggcggaaat cccacggaat atcgggcgcg caaaatggtg ccagaaagcc gccgcttgcc gcatcgacat ggatatcgat atcaagaccc ttggtctttt gcagcttgtc cagcgcatcc tgcaacggtt cgggaaattc ataattgccg gtataggtca cgccgaaagt aggcacgacg ccgatggtat tttcatcaac catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVDENTIGV VPTFGVTYTG NYEFPEPLQD ALDKLQKTKG LDIDIHVDAA SGGFLAPFCA PDIPWDFRLP RVKSISASGH KYGLAPLGCG WVVWRDKEAL PEELIFNVDY LGGQVGTFAI NFSRPAGQVI SQYYEFMRLG REGYTKVQQA AYRVAQYIAR EIEPLGPYEF ICAGEEKGGI PAVCFRIREG EDPGYSLYDL SERLRLTGWQ VPAFALSGKA SDITVMRVMC RCGFEMDLAA LFIRDFKAGI EFFKSHPSPK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggttgatgaa aataccatcg gcgtcgtgcc tactttcggc gtgacctata ccggcaatta tgaatttccc gaaccgttgc aggatgcgct ggacaagctg caaaagacca agggtcttga tatcgatatc catgtcgatg cggcaagcgg cggctttctg gcaccatttt gcgcgcccga tattccgtgg gatttccgcc tgccgcgcgt gaagtcgatc agcgcgtcag gccataaata cggtctcgca ccgcttggct gtggctgggt tgtctggcgc gacaaagagg ccctgccgga agagctgatc ttcaatgtgg attatctcgg tggacaggtt ggcactttcg caatcaattt ctcgcggcct gccggtcagg tcatttcaca atattacgaa ttcatgcgtc ttggccgcga aggctacacc aaggtgcagc aggcggccta ccgcgtggcg caatatatag ccagggagat cgagccgctt gggccttatg aattcatctg cgcgggcgag gagaaaggcg gcattcccgc cgtctgtttc cgcatccgtg aaggcgaaga ccccggttat tcgctctatg atctgtcgga acggctgcgt ctgaccggat ggcaggttcc cgctttcgct ctttcgggca aggcgtcgga tatcactgtc atgcgcgtca tgtgccgctg cggtttcgag atggatctgg ctgcactctt cattcgggat ttcaaggctg ggatcgagtt cttcaaatcg catccttcac cgaagaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa