BrabA.00121.a

Biotin synthase:Elongator protein 3/MiaB/NifB:Radical SAM

CENTER ID: BrabA.00121.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00121.a.A2.GE28070 Created from Decode construct for feature BrabA.00121.a 1 319
BrabA.00121.a.A3.GE28071 Created from Decode construct for feature BrabA.00121.a 1 325
BrabA.00121.a.A4.GE28072 Created from Decode construct for feature BrabA.00121.a 16 328
BrabA.00121.a.A5.GE28073 Created from Decode construct for feature BrabA.00121.a 16 319
BrabA.00121.a.A6.GE28074 Created from Decode construct for feature BrabA.00121.a 16 325
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00121.a.A4.PS00952 Created from Decode construct for feature BrabA.00121.a 16 328
BrabA.00121.a.A3.PW27964 Created from Decode construct for feature BrabA.00121.a 1 325
BrabA.00121.a.A4.PW27965 Created from Decode construct for feature BrabA.00121.a 16 328
BrabA.00121.a.A6.PW27967 Created from Decode construct for feature BrabA.00121.a 16 325
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_129979
PATRIC ID: fig|359391.4.peg.3039
RefSeq: YP_418916
UniProt: Q2YKB8
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPDRGGENGA SCSVGRWSAE EARAIYNLPF NDLLFRAHGL HRENFDPNRI QLSKLLNIKT GGCPEDCGYC SQSASAENGL KASKLMEIET VLEEARKAKA AGATRYCMGA AWRSPKDRDM PALTHMIESV KAMGLETCMT LGMLDSDKAE KLADAGLDYY NHNIDTSERF YPAVITTRSF EDRLDTLANV RNAGIKVCSG GILGLGEEAE DRIDMLVTLA NLPEPPESVP INMLIPMPGT RLAKAAPVDP LEFVRVVALA RILMPKSHVR LTAGRTAMSD EMQALCFFAG ANSLFMGDTL LTAANPGDDR DSSLLRRLGI QAETEQPA
NT Sequence
ATGCCAGATC GTGGAGGCGA GAACGGGGCA TCCTGTTCGG TGGGCCGTTG GAGCGCAGAA GAAGCGCGGG CAATCTATAA TCTGCCGTTC AATGATCTGC TCTTTCGCGC CCACGGCCTG CACCGCGAGA ATTTTGATCC GAACCGCATC CAGCTCAGCA AGCTTCTCAA CATCAAGACG GGTGGATGCC CGGAAGATTG CGGTTATTGC AGCCAGTCTG CTTCTGCGGA AAATGGCCTG AAAGCCTCCA AACTCATGGA AATCGAAACC GTTCTGGAGG AGGCGCGCAA GGCCAAGGCT GCGGGTGCCA CGCGCTATTG CATGGGGGCA GCTTGGCGCA GCCCCAAGGA TCGCGACATG CCCGCCCTCA CCCATATGAT CGAAAGCGTG AAGGCAATGG GCCTGGAAAC CTGCATGACG CTGGGGATGC TGGATAGCGA CAAGGCGGAA AAACTCGCCG ATGCAGGCCT CGATTATTAC AATCACAATA TCGATACGTC CGAGCGTTTT TATCCCGCAG TCATCACCAC GCGCAGTTTT GAAGATCGGC TCGATACGCT TGCAAATGTG CGCAATGCGG GCATCAAGGT TTGCAGCGGC GGTATTCTGG GGCTTGGCGA AGAGGCCGAA GATCGCATCG ACATGCTGGT AACACTGGCC AACCTGCCGG AACCGCCGGA GAGCGTGCCG ATCAATATGC TGATCCCCAT GCCGGGCACA CGGCTGGCAA AGGCCGCGCC AGTCGATCCG CTTGAATTTG TCCGTGTGGT CGCGCTGGCG CGCATTCTCA TGCCGAAATC CCATGTGCGC CTGACGGCTG GCCGTACCGC CATGAGCGAC GAGATGCAGG CGCTCTGCTT CTTTGCCGGA GCCAATTCGC TATTTATGGG CGACACCTTG CTCACAGCCG CCAACCCCGG CGACGACCGC GATTCCAGCC TGCTGCGCCG CCTCGGCATA CAGGCGGAAA CGGAGCAGCC TGCG
Details for BrabA.00121.a.A2.GE28070
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MPDRGGENGA SCSVGRWSAE EARAIYNLPF NDLLFRAHGL HRENFDPNRI QLSKLLNIKT GGCPEDCGYC SQSASAENGL KASKLMEIET VLEEARKAKA AGATRYCMGA AWRSPKDRDM PALTHMIESV KAMGLETCMT LGMLDSDKAE KLADAGLDYY NHNIDTSERF YPAVITTRSF EDRLDTLANV RNAGIKVCSG GILGLGEEAE DRIDMLVTLA NLPEPPESVP INMLIPMPGT RLAKAAPVDP LEFVRVVALA RILMPKSHVR LTAGRTAMSD EMQALCFFAG ANSLFMGDTL LTAANPGDDR DSSLLRRLG
Validated NT Sequence
tattagccga ggcggcgcag caggctggaa tcgcggtcgt cgccggggtt ggcggctgtg agcaaggtgt cgcccataaa tagcgaattg gctccggcaa agaagcagag cgcctgcatc tcgtcgctca tggcggtacg gccagccgtc aggcgcacat gggatttcgg catgagaatg cgcgccagcg cgaccacacg gacaaattca agcggatcga ctggcgcggc ctttgccagc cgtgtgcccg gcatggggat cagcatattg atcggcacgc tctccggcgg ttccggcagg ttggccagtg ttaccagcat gtcgatgcga tcttcggcct cttcgccaag ccccagaata ccgccgctgc aaaccttgat gcccgcattg cgcacatttg caagcgtatc gagccgatct tcaaaactgc gcgtggtgat gactgcggga taaaaacgct cggacgtatc gatattgtga ttgtaataat cgaggcctgc atcggcgagt ttttccgcct tgtcgctatc cagcatcccc agcgtcatgc aggtttccag gcccattgcc ttcacgcttt cgatcatatg ggtgagggcg ggcatgtcgc gatccttggg gctgcgccaa gctgccccca tgcaatagcg cgtggcaccc gcagccttgg ccttgcgcgc ctcctccaga acggtttcga tttccatgag tttggaggct ttcaggccat tttccgcaga agcagactgg ctgcaataac cgcaatcttc cgggcatcca cccgtcttga tgttgagaag cttgctgagc tggatgcggt tcggatcaaa attctcgcgg tgcaggccgt gggcgcgaaa gagcagatca ttgaacggca gattatagat tgcccgcgct tcttctgcgc tccaacggcc caccgaacag gatgccccgt tctcgcctcc acgatctggc atcgaaccag gaccctgggt ctgagcttcc aggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPDRGGENG ASCSVGRWSA EEARAIYNLP FNDLLFRAHG LHRENFDPNR IQLSKLLNIK TGGCPEDCGY CSQSASAENG LKASKLMEIE TVLEEARKAK AAGATRYCMG AAWRSPKDRD MPALTHMIES VKAMGLETCM TLGMLDSDKA EKLADAGLDY YNHNIDTSER FYPAVITTRS FEDRLDTLAN VRNAGIKVCS GGILGLGEEA EDRIDMLVTL ANLPEPPESV PINMLIPMPG TRLAKAAPVD PLEFVRVVAL ARILMPKSHV RLTAGRTAMS DEMQALCFFA GANSLFMGDT LLTAANPGDD RDSSLLRRLG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccagatcgt ggaggcgaga acggggcatc ctgttcggtg ggccgttgga gcgcagaaga agcgcgggca atctataatc tgccgttcaa tgatctgctc tttcgcgccc acggcctgca ccgcgagaat tttgatccga accgcatcca gctcagcaag cttctcaaca tcaagacggg tggatgcccg gaagattgcg gttattgcag ccagtctgct tctgcggaaa atggcctgaa agcctccaaa ctcatggaaa tcgaaaccgt tctggaggag gcgcgcaagg ccaaggctgc gggtgccacg cgctattgca tgggggcagc ttggcgcagc cccaaggatc gcgacatgcc cgccctcacc catatgatcg aaagcgtgaa ggcaatgggc ctggaaacct gcatgacgct ggggatgctg gatagcgaca aggcggaaaa actcgccgat gcaggcctcg attattacaa tcacaatatc gatacgtccg agcgttttta tcccgcagtc atcaccacgc gcagttttga agatcggctc gatacgcttg caaatgtgcg caatgcgggc atcaaggttt gcagcggcgg tattctgggg cttggcgaag aggccgaaga tcgcatcgac atgctggtaa cactggccaa cctgccggaa ccgccggaga gcgtgccgat caatatgctg atccccatgc cgggcacacg gctggcaaag gccgcgccag tcgatccgct tgaatttgtc cgtgtggtcg cgctggcgcg cattctcatg ccgaaatccc atgtgcgcct gacggctggc cgtaccgcca tgagcgacga gatgcaggcg ctctgcttct ttgccggagc caattcgcta tttatgggcg acaccttgct cacagccgcc aaccccggcg acgaccgcga ttccagcctg ctgcgccgcc tcggcaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BrabA.00121.a.A3.GE28071
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 37kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMPDRGGE NGASCSVGRW SAEEARAIYN LPFNDLLFRA HGLHRENFDP NRIQLSKLLN IKTGGCPEDC GYCSQSASAE NGLKASKLME IETVLEEARK AKAAGATRYC MGAAWRSPKD RDMPALTHMI ESVKAMGLET CMTLGMLDSD KAEKLADAGL DYYNHNIDTS ERFYPAVITT RSFEDRLDTL ANVRNAGIKV CSGGILGLGE EAEDRIDMLV TLANLPEPPE SVPINMLIPM PGTRLAKAAP VDPLEFVRVV ALARILMPKS HVRLTAGRTA MSDEMQALCF FAGANSLFMG DTLLTAANPG DDRDSSLLRR LGIQAETE
Validated NT Sequence
ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatgc cagatcgtgg aggcgagaac ggggcatcct gttcggtggg ccgttggagc gcagaagaag cgcgggcaat ctataatctg ccgttcaatg atctgctctt tcgcgcccac ggcctgcacc gcgagaattt tgatccgaac cgcatccagc tcagcaagct tctcaacatc aagacgggtg gatgcccgga agattgcggt tattgcagcc agtctgcttc tgcggaaaat ggcctgaaag cctccaaact catggaaatc gaaaccgttc tggaggaggc gcgcaaggcc aaggctgcgg gtgccacgcg ctattgcatg ggggcagctt ggcgcagccc caaggatcgc gacatgcccg ccctcaccca tatgatcgaa agcgtgaagg caatgggcct ggaaacctgc atgacgctgg ggatgctgga tagcgacaag gcggaaaaac tcgccgatgc aggcctcgat tattacaatc acaatatcga tacgtccgag cgtttttatc ccgcagtcat caccacgcgc agttttgaag atcggctcga tacgcttgca aatgtgcgca atgcgggcat caaggtttgc agcggcggta ttctggggct tggcgaagag gccgaagatc gcatcgacat gctggtaaca ctggccaacc tgccggaacc gccggagagc gtgccgatca atatgctgat ccccatgccg ggcacacggc tggcaaaggc cgcgccagtc gatccgcttg aatttgtccg tgtggtcgcg ctggcgcgca ttctcatgcc gaaatcccat gtgcgcctga cggctggccg taccgccatg agcgacgaga tgcaggcgct ctgcttcttt gccggagcca attcgctatt tatgggcgac accttgctca cagccgccaa ccccggcgac gaccgcgatt ccagcctgct gcgccgcctc ggcatacagg cggaaacgga gtaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPDRGGENG ASCSVGRWSA EEARAIYNLP FNDLLFRAHG LHRENFDPNR IQLSKLLNIK TGGCPEDCGY CSQSASAENG LKASKLMEIE TVLEEARKAK AAGATRYCMG AAWRSPKDRD MPALTHMIES VKAMGLETCM TLGMLDSDKA EKLADAGLDY YNHNIDTSER FYPAVITTRS FEDRLDTLAN VRNAGIKVCS GGILGLGEEA EDRIDMLVTL ANLPEPPESV PINMLIPMPG TRLAKAAPVD PLEFVRVVAL ARILMPKSHV RLTAGRTAMS DEMQALCFFA GANSLFMGDT LLTAANPGDD RDSSLLRRLG IQAETE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccagatcgt ggaggcgaga acggggcatc ctgttcggtg ggccgttgga gcgcagaaga agcgcgggca atctataatc tgccgttcaa tgatctgctc tttcgcgccc acggcctgca ccgcgagaat tttgatccga accgcatcca gctcagcaag cttctcaaca tcaagacggg tggatgcccg gaagattgcg gttattgcag ccagtctgct tctgcggaaa atggcctgaa agcctccaaa ctcatggaaa tcgaaaccgt tctggaggag gcgcgcaagg ccaaggctgc gggtgccacg cgctattgca tgggggcagc ttggcgcagc cccaaggatc gcgacatgcc cgccctcacc catatgatcg aaagcgtgaa ggcaatgggc ctggaaacct gcatgacgct ggggatgctg gatagcgaca aggcggaaaa actcgccgat gcaggcctcg attattacaa tcacaatatc gatacgtccg agcgttttta tcccgcagtc atcaccacgc gcagttttga agatcggctc gatacgcttg caaatgtgcg caatgcgggc atcaaggttt gcagcggcgg tattctgggg cttggcgaag aggccgaaga tcgcatcgac atgctggtaa cactggccaa cctgccggaa ccgccggaga gcgtgccgat caatatgctg atccccatgc cgggcacacg gctggcaaag gccgcgccag tcgatccgct tgaatttgtc cgtgtggtcg cgctggcgcg cattctcatg ccgaaatccc atgtgcgcct gacggctggc cgtaccgcca tgagcgacga gatgcaggcg ctctgcttct ttgccggagc caattcgcta tttatgggcg acaccttgct cacagccgcc aaccccggcg acgaccgcga ttccagcctg ctgcgccgcc tcggcataca ggcggaaacg gagaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BrabA.00121.a.A4.GE28072
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 36kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MRWSAEEARA IYNLPFNDLL FRAHGLHREN FDPNRIQLSK LLNIKTGGCP EDCGYCSQSA SAENGLKASK LMEIETVLEE ARKAKAAGAT RYCMGAAWRS PKDRDMPALT HMIESVKAMG LETCMTLGML DSDKAEKLAD AGLDYYNHNI DTSERFYPAV ITTRSFEDRL DTLANVRNAG IKVCSGGILG LGEEAEDRID MLVTLANLPE PPESVPINML IPMPGTRLAK AAPVDPLEFV RVVALARILM PKSHVRLTAG RTAMSDEMQA LCFFAGANSL FMGDTLLTAA NPGDDRDSSL LRRLGIQAET EQPA
Validated NT Sequence
ggtaccctgg aagctcagac ccagggtcct ggttcgatgc gttggagcgc agaagaagcg cgggcaatct ataatctgcc gttcaatgat ctgctctttc gcgcccacgg cctgcaccgc gagaattttg atccgaaccg catccagctc agcaagcttc tcaacatcaa gacgggtgga tgcccggaag attgcggtta ttgcagccag tctgcttctg cggaaaatgg cctgaaagcc tccaaactca tggaaatcga aaccgttctg gaggaggcgc gcaaggccaa ggctgcgggt gccacgcgct attgcatggg ggcagcttgg cgcagcccca aggatcgcga catgcccgcc ctcacccata tgatcgaaag cgtgaaggca atgggcctgg aaacctgcat gacgctgggg atgctggata gcgacaaggc ggaaaaactc gccgatgcag gcctcgatta ttacaatcac aatatcgata cgtccgagcg tttttatccc gcagtcatca ccacgcgcag ttttgaagat cggctcgata cgcttgcaaa tgtgcgcaat gcgggcatca aggtttgcag cggcggtatt ctggggcttg gcgaagaggc cgaagatcgc atcgacatgc tggtaacact ggccaacctg ccggaaccgc cggagagcgt gccgatcaat atgctgatcc ccatgccggg cacacggctg gcaaaggccg cgccagtcga tccgcttgaa tttgtccgtg tggtcgcgct ggcgcgcatt ctcatgccga aatcccatgt gcgcctgacg gctggccgta ccgccatgag cgacgagatg caggcgctct gcttctttgc cggagccaat tcgctattta tgggcgacac cttgctcaca gccgccaacc ccggcgacga ccgcgattcc agcctgctgc gccgcctcgg catacaggcg gaaacggagc agcctgcgta ataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRWSAEEAR AIYNLPFNDL LFRAHGLHRE NFDPNRIQLS KLLNIKTGGC PEDCGYCSQS ASAENGLKAS KLMEIETVLE EARKAKAAGA TRYCMGAAWR SPKDRDMPAL THMIESVKAM GLETCMTLGM LDSDKAEKLA DAGLDYYNHN IDTSERFYPA VITTRSFEDR LDTLANVRNA GIKVCSGGIL GLGEEAEDRI DMLVTLANLP EPPESVPINM LIPMPGTRLA KAAPVDPLEF VRVVALARIL MPKSHVRLTA GRTAMSDEMQ ALCFFAGANS LFMGDTLLTA ANPGDDRDSS LLRRLGIQAE TEQPA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgttggagc gcagaagaag cgcgggcaat ctataatctg ccgttcaatg atctgctctt tcgcgcccac ggcctgcacc gcgagaattt tgatccgaac cgcatccagc tcagcaagct tctcaacatc aagacgggtg gatgcccgga agattgcggt tattgcagcc agtctgcttc tgcggaaaat ggcctgaaag cctccaaact catggaaatc gaaaccgttc tggaggaggc gcgcaaggcc aaggctgcgg gtgccacgcg ctattgcatg ggggcagctt ggcgcagccc caaggatcgc gacatgcccg ccctcaccca tatgatcgaa agcgtgaagg caatgggcct ggaaacctgc atgacgctgg ggatgctgga tagcgacaag gcggaaaaac tcgccgatgc aggcctcgat tattacaatc acaatatcga tacgtccgag cgtttttatc ccgcagtcat caccacgcgc agttttgaag atcggctcga tacgcttgca aatgtgcgca atgcgggcat caaggtttgc agcggcggta ttctggggct tggcgaagag gccgaagatc gcatcgacat gctggtaaca ctggccaacc tgccggaacc gccggagagc gtgccgatca atatgctgat ccccatgccg ggcacacggc tggcaaaggc cgcgccagtc gatccgcttg aatttgtccg tgtggtcgcg ctggcgcgca ttctcatgcc gaaatcccat gtgcgcctga cggctggccg taccgccatg agcgacgaga tgcaggcgct ctgcttcttt gccggagcca attcgctatt tatgggcgac accttgctca cagccgccaa ccccggcgac gaccgcgatt ccagcctgct gcgccgcctc ggcatacagg cggaaacgga gcagcctgcg aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BrabA.00121.a.A5.GE28073
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 35kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMRWSAEE ARAIYNLPFN DLLFRAHGLH RENFDPNRIQ LSKLLNIKTG GCPEDCGYCS QSASAENGLK ASKLMEIETV LEEARKAKAA GATRYCMGAA WRSPKDRDMP ALTHMIESVK AMGLETCMTL GMLDSDKAEK LADAGLDYYN HNIDTSERFY PAVITTRSFE DRLDTLANVR NAGIKVCSGG ILGLGEEAED RIDMLVTLAN LPEPPESVPI NMLIPMPGTR LAKAAPVDPL EFVRVVALAR ILMPKSHVRL TAGRTAMSDE MQALCFFAGA NSLFMGDTLL TAANPGDDRD SSLLRRLG
Validated NT Sequence
ttattagccg aggcggcgca gcaggctgga atcgcggtcg tcgccggggt tggcggctgt gagcaaggtg tcgcccataa atagcgaatt ggctccggca aagaagcaga gcgcctgcat ctcgtcgctc atggcggtac ggccagccgt caggcgcaca tgggatttcg gcatgagaat gcgcgccagc gcgaccacac ggacaaattc aagcggatcg actggcgcgg cctttgccag ccgtgtgccc ggcatgggga tcagcatatt gatcggcacg ctctccggcg gttccggcag gttggccagt gttaccagca tgtcgatgcg atcttcggcc tcttcgccaa gccccagaat accgccgctg caaaccttga tgcccgcatt gcgcacattt gcaagcgtat cgagccgatc ttcaaaactg cgcgtggtga tgactgcggg ataaaaacgc tcggacgtat cgatattgtg attgtaataa tcgaggcctg catcggcgag tttttccgcc ttgtcgctat ccagcatccc cagcgtcatg caggtttcca ggcccattgc cttcacgctt tcgatcatat gggtgagggc gggcatgtcg cgatccttgg ggctgcgcca agctgccccc atgcaatagc gcgtggcacc cgcagccttg gccttgcgcg cctcctccag aacggtttcg atttccatga gtttggaggc tttcaggcca ttttccgcag aagcagactg gctgcaataa ccgcaatctt ccgggcatcc acccgtcttg atgttgagaa gcttgctgag ctggatgcgg ttcggatcaa aattctcgcg gtgcaggccg tgggcgcgaa agagcagatc attgaacggc agattataga ttgcccgcgc ttcttctgcg ctccaacgca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag cca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRWSAEEAR AIYNLPFNDL LFRAHGLHRE NFDPNRIQLS KLLNIKTGGC PEDCGYCSQS ASAENGLKAS KLMEIETVLE EARKAKAAGA TRYCMGAAWR SPKDRDMPAL THMIESVKAM GLETCMTLGM LDSDKAEKLA DAGLDYYNHN IDTSERFYPA VITTRSFEDR LDTLANVRNA GIKVCSGGIL GLGEEAEDRI DMLVTLANLP EPPESVPINM LIPMPGTRLA KAAPVDPLEF VRVVALARIL MPKSHVRLTA GRTAMSDEMQ ALCFFAGANS LFMGDTLLTA ANPGDDRDSS LLRRLG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgttggagc gcagaagaag cgcgggcaat ctataatctg ccgttcaatg atctgctctt tcgcgcccac ggcctgcacc gcgagaattt tgatccgaac cgcatccagc tcagcaagct tctcaacatc aagacgggtg gatgcccgga agattgcggt tattgcagcc agtctgcttc tgcggaaaat ggcctgaaag cctccaaact catggaaatc gaaaccgttc tggaggaggc gcgcaaggcc aaggctgcgg gtgccacgcg ctattgcatg ggggcagctt ggcgcagccc caaggatcgc gacatgcccg ccctcaccca tatgatcgaa agcgtgaagg caatgggcct ggaaacctgc atgacgctgg ggatgctgga tagcgacaag gcggaaaaac tcgccgatgc aggcctcgat tattacaatc acaatatcga tacgtccgag cgtttttatc ccgcagtcat caccacgcgc agttttgaag atcggctcga tacgcttgca aatgtgcgca atgcgggcat caaggtttgc agcggcggta ttctggggct tggcgaagag gccgaagatc gcatcgacat gctggtaaca ctggccaacc tgccggaacc gccggagagc gtgccgatca atatgctgat ccccatgccg ggcacacggc tggcaaaggc cgcgccagtc gatccgcttg aatttgtccg tgtggtcgcg ctggcgcgca ttctcatgcc gaaatcccat gtgcgcctga cggctggccg taccgccatg agcgacgaga tgcaggcgct ctgcttcttt gccggagcca attcgctatt tatgggcgac accttgctca cagccgccaa ccccggcgac gaccgcgatt ccagcctgct gcgccgcctc ggcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BrabA.00121.a.A6.GE28074
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMRWSAEE ARAIYNLPFN DLLFRAHGLH RENFDPNRIQ LSKLLNIKTG GCPEDCGYCS QSASAENGLK ASKLMEIETV LEEARKAKAA GATRYCMGAA WRSPKDRDMP ALTHMIESVK AMGLETCMTL GMLDSDKAEK LADAGLDYYN HNIDTSERFY PAVITTRSFE DRLDTLANVR NAGIKVCSGG ILGLGEEAED RIDMLVTLAN LPEPPESVPI NMLIPMPGTR LAKAAPVDPL EFVRVVALAR ILMPKSHVRL TAGRTAMSDE MQALCFFAGA NSLFMGDTLL TAANPGDDRD SSLLRRLGIQ AETE
Validated NT Sequence
ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgcgttg gagcgcagaa gaagcgcggg caatctataa tctgccgttc aatgatctgc tctttcgcgc ccacggcctg caccgcgaga attttgatcc gaaccgcatc cagctcagca agcttctcaa catcaagacg ggtggatgcc cggaagattg cggttattgc agccagtctg cttctgcgga aaatggcctg aaagcctcca aactcatgga aatcgaaacc gttctggagg aggcgcgcaa ggccaaggct gcgggtgcca cgcgctattg catgggggca gcttggcgca gccccaagga tcgcgacatg cccgccctca cccatatgat cgaaagcgtg aaggcaatgg gcctggaaac ctgcatgacg ctggggatgc tggatagcga caaggcggaa aaactcgccg atgcaggcct cgattattac aatcacaata tcgatacgtc cgagcgtttt tatcccgcag tcatcaccac gcgcagtttt gaagatcggc tcgatacgct tgcaaatgtg cgcaatgcgg gcatcaaggt ttgcagcggc ggtattctgg ggcttggcga agaggccgaa gatcgcatcg acatgctggt aacactggcc aacctgccgg aaccgccgga gagcgtgccg atcaatatgc tgatccccat gccgggcaca cggctggcaa aggccgcgcc agtcgatccg cttgaatttg tccgtgtggt cgcgctggcg cgcattctca tgccgaaatc ccatgtgcgc ctgacggctg gccgtaccgc catgagcgac gagatgcagg cgctctgctt ctttgccgga gccaattcgc tatttatggg cgacaccttg ctcacagccg ccaaccccgg cgacgaccgc gattccagcc tgctgcgccg cctcggcata caggcggaaa cggagtaata a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRWSAEEAR AIYNLPFNDL LFRAHGLHRE NFDPNRIQLS KLLNIKTGGC PEDCGYCSQS ASAENGLKAS KLMEIETVLE EARKAKAAGA TRYCMGAAWR SPKDRDMPAL THMIESVKAM GLETCMTLGM LDSDKAEKLA DAGLDYYNHN IDTSERFYPA VITTRSFEDR LDTLANVRNA GIKVCSGGIL GLGEEAEDRI DMLVTLANLP EPPESVPINM LIPMPGTRLA KAAPVDPLEF VRVVALARIL MPKSHVRLTA GRTAMSDEMQ ALCFFAGANS LFMGDTLLTA ANPGDDRDSS LLRRLGIQAE TE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgttggagc gcagaagaag cgcgggcaat ctataatctg ccgttcaatg atctgctctt tcgcgcccac ggcctgcacc gcgagaattt tgatccgaac cgcatccagc tcagcaagct tctcaacatc aagacgggtg gatgcccgga agattgcggt tattgcagcc agtctgcttc tgcggaaaat ggcctgaaag cctccaaact catggaaatc gaaaccgttc tggaggaggc gcgcaaggcc aaggctgcgg gtgccacgcg ctattgcatg ggggcagctt ggcgcagccc caaggatcgc gacatgcccg ccctcaccca tatgatcgaa agcgtgaagg caatgggcct ggaaacctgc atgacgctgg ggatgctgga tagcgacaag gcggaaaaac tcgccgatgc aggcctcgat tattacaatc acaatatcga tacgtccgag cgtttttatc ccgcagtcat caccacgcgc agttttgaag atcggctcga tacgcttgca aatgtgcgca atgcgggcat caaggtttgc agcggcggta ttctggggct tggcgaagag gccgaagatc gcatcgacat gctggtaaca ctggccaacc tgccggaacc gccggagagc gtgccgatca atatgctgat ccccatgccg ggcacacggc tggcaaaggc cgcgccagtc gatccgcttg aatttgtccg tgtggtcgcg ctggcgcgca ttctcatgcc gaaatcccat gtgcgcctga cggctggccg taccgccatg agcgacgaga tgcaggcgct ctgcttcttt gccggagcca attcgctatt tatgggcgac accttgctca cagccgccaa ccccggcgac gaccgcgatt ccagcctgct gcgccgcctc ggcatacagg cggaaacgga gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BrabA.00121.a.A4.PS00952
PURIFICATION DATe: 2/1/2011
CONCENTRATION: 26.23mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 16
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MRWSAEEARA IYNLPFNDLL FRAHGLHREN FDPNRIQLSK LLNIKTGGCP EDCGYCSQSA SAENGLKASK LMEIETVLEE ARKAKAAGAT RYCMGAAWRS PKDRDMPALT HMIESVKAMG LETCMTLGML DSDKAEKLAD AGLDYYNHNI DTSERFYPAV ITTRSFEDRL DTLANVRNAG IKVCSGGILG LGEEAEDRID MLVTLANLPE PPESVPINML IPMPGTRLAK AAPVDPLEFV RVVALARILM PKSHVRLTAG RTAMSDEMQA LCFFAGANSL FMGDTLLTAA NPGDDRDSSL LRRLGIQAET EQPA
Validated NT Sequence
ggtaccctgg aagctcagac ccagggtcct ggttcgatgc gttggagcgc agaagaagcg cgggcaatct ataatctgcc gttcaatgat ctgctctttc gcgcccacgg cctgcaccgc gagaattttg atccgaaccg catccagctc agcaagcttc tcaacatcaa gacgggtgga tgcccggaag attgcggtta ttgcagccag tctgcttctg cggaaaatgg cctgaaagcc tccaaactca tggaaatcga aaccgttctg gaggaggcgc gcaaggccaa ggctgcgggt gccacgcgct attgcatggg ggcagcttgg cgcagcccca aggatcgcga catgcccgcc ctcacccata tgatcgaaag cgtgaaggca atgggcctgg aaacctgcat gacgctgggg atgctggata gcgacaaggc ggaaaaactc gccgatgcag gcctcgatta ttacaatcac aatatcgata cgtccgagcg tttttatccc gcagtcatca ccacgcgcag ttttgaagat cggctcgata cgcttgcaaa tgtgcgcaat gcgggcatca aggtttgcag cggcggtatt ctggggcttg gcgaagaggc cgaagatcgc atcgacatgc tggtaacact ggccaacctg ccggaaccgc cggagagcgt gccgatcaat atgctgatcc ccatgccggg cacacggctg gcaaaggccg cgccagtcga tccgcttgaa tttgtccgtg tggtcgcgct ggcgcgcatt ctcatgccga aatcccatgt gcgcctgacg gctggccgta ccgccatgag cgacgagatg caggcgctct gcttctttgc cggagccaat tcgctattta tgggcgacac cttgctcaca gccgccaacc ccggcgacga ccgcgattcc agcctgctgc gccgcctcgg catacaggcg gaaacggagc agcctgcgta ataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRWSAEEAR AIYNLPFNDL LFRAHGLHRE NFDPNRIQLS KLLNIKTGGC PEDCGYCSQS ASAENGLKAS KLMEIETVLE EARKAKAAGA TRYCMGAAWR SPKDRDMPAL THMIESVKAM GLETCMTLGM LDSDKAEKLA DAGLDYYNHN IDTSERFYPA VITTRSFEDR LDTLANVRNA GIKVCSGGIL GLGEEAEDRI DMLVTLANLP EPPESVPINM LIPMPGTRLA KAAPVDPLEF VRVVALARIL MPKSHVRLTA GRTAMSDEMQ ALCFFAGANS LFMGDTLLTA ANPGDDRDSS LLRRLGIQAE TEQPA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGTTGGAGC GCAGAAGAAG CGCGGGCAAT CTATAATCTG CCGTTCAATG ATCTGCTCTT TCGCGCCCAC GGCCTGCACC GCGAGAATTT TGATCCGAAC CGCATCCAGC TCAGCAAGCT TCTCAACATC AAGACGGGTG GATGCCCGGA AGATTGCGGT TATTGCAGCC AGTCTGCTTC TGCGGAAAAT GGCCTGAAAG CCTCCAAACT CATGGAAATC GAAACCGTTC TGGAGGAGGC GCGCAAGGCC AAGGCTGCGG GTGCCACGCG CTATTGCATG GGGGCAGCTT GGCGCAGCCC CAAGGATCGC GACATGCCCG CCCTCACCCA TATGATCGAA AGCGTGAAGG CAATGGGCCT GGAAACCTGC ATGACGCTGG GGATGCTGGA TAGCGACAAG GCGGAAAAAC TCGCCGATGC AGGCCTCGAT TATTACAATC ACAATATCGA TACGTCCGAG CGTTTTTATC CCGCAGTCAT CACCACGCGC AGTTTTGAAG ATCGGCTCGA TACGCTTGCA AATGTGCGCA ATGCGGGCAT CAAGGTTTGC AGCGGCGGTA TTCTGGGGCT TGGCGAAGAG GCCGAAGATC GCATCGACAT GCTGGTAACA CTGGCCAACC TGCCGGAACC GCCGGAGAGC GTGCCGATCA ATATGCTGAT CCCCATGCCG GGCACACGGC TGGCAAAGGC CGCGCCAGTC GATCCGCTTG AATTTGTCCG TGTGGTCGCG CTGGCGCGCA TTCTCATGCC GAAATCCCAT GTGCGCCTGA CGGCTGGCCG TACCGCCATG AGCGACGAGA TGCAGGCGCT CTGCTTCTTT GCCGGAGCCA ATTCGCTATT TATGGGCGAC ACCTTGCTCA CAGCCGCCAA CCCCGGCGAC GACCGCGATT CCAGCCTGCT GCGCCGCCTC GGCATACAGG CGGAAACGGA GCAGCCTGCG AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA
Details for BrabA.00121.a.A3.PW27964
PURIFICATION DATe: 2/8/2010
CONCENTRATION: 26.84mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 12
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMPDRGGE NGASCSVGRW SAEEARAIYN LPFNDLLFRA HGLHRENFDP NRIQLSKLLN IKTGGCPEDC GYCSQSASAE NGLKASKLME IETVLEEARK AKAAGATRYC MGAAWRSPKD RDMPALTHMI ESVKAMGLET CMTLGMLDSD KAEKLADAGL DYYNHNIDTS ERFYPAVITT RSFEDRLDTL ANVRNAGIKV CSGGILGLGE EAEDRIDMLV TLANLPEPPE SVPINMLIPM PGTRLAKAAP VDPLEFVRVV ALARILMPKS HVRLTAGRTA MSDEMQALCF FAGANSLFMG DTLLTAANPG DDRDSSLLRR LGIQAETE
Validated NT Sequence
ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatgc cagatcgtgg aggcgagaac ggggcatcct gttcggtggg ccgttggagc gcagaagaag cgcgggcaat ctataatctg ccgttcaatg atctgctctt tcgcgcccac ggcctgcacc gcgagaattt tgatccgaac cgcatccagc tcagcaagct tctcaacatc aagacgggtg gatgcccgga agattgcggt tattgcagcc agtctgcttc tgcggaaaat ggcctgaaag cctccaaact catggaaatc gaaaccgttc tggaggaggc gcgcaaggcc aaggctgcgg gtgccacgcg ctattgcatg ggggcagctt ggcgcagccc caaggatcgc gacatgcccg ccctcaccca tatgatcgaa agcgtgaagg caatgggcct ggaaacctgc atgacgctgg ggatgctgga tagcgacaag gcggaaaaac tcgccgatgc aggcctcgat tattacaatc acaatatcga tacgtccgag cgtttttatc ccgcagtcat caccacgcgc agttttgaag atcggctcga tacgcttgca aatgtgcgca atgcgggcat caaggtttgc agcggcggta ttctggggct tggcgaagag gccgaagatc gcatcgacat gctggtaaca ctggccaacc tgccggaacc gccggagagc gtgccgatca atatgctgat ccccatgccg ggcacacggc tggcaaaggc cgcgccagtc gatccgcttg aatttgtccg tgtggtcgcg ctggcgcgca ttctcatgcc gaaatcccat gtgcgcctga cggctggccg taccgccatg agcgacgaga tgcaggcgct ctgcttcttt gccggagcca attcgctatt tatgggcgac accttgctca cagccgccaa ccccggcgac gaccgcgatt ccagcctgct gcgccgcctc ggcatacagg cggaaacgga gtaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPDRGGENG ASCSVGRWSA EEARAIYNLP FNDLLFRAHG LHRENFDPNR IQLSKLLNIK TGGCPEDCGY CSQSASAENG LKASKLMEIE TVLEEARKAK AAGATRYCMG AAWRSPKDRD MPALTHMIES VKAMGLETCM TLGMLDSDKA EKLADAGLDY YNHNIDTSER FYPAVITTRS FEDRLDTLAN VRNAGIKVCS GGILGLGEEA EDRIDMLVTL ANLPEPPESV PINMLIPMPG TRLAKAAPVD PLEFVRVVAL ARILMPKSHV RLTAGRTAMS DEMQALCFFA GANSLFMGDT LLTAANPGDD RDSSLLRRLG IQAETE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCAGATCGT GGAGGCGAGA ACGGGGCATC CTGTTCGGTG GGCCGTTGGA GCGCAGAAGA AGCGCGGGCA ATCTATAATC TGCCGTTCAA TGATCTGCTC TTTCGCGCCC ACGGCCTGCA CCGCGAGAAT TTTGATCCGA ACCGCATCCA GCTCAGCAAG CTTCTCAACA TCAAGACGGG TGGATGCCCG GAAGATTGCG GTTATTGCAG CCAGTCTGCT TCTGCGGAAA ATGGCCTGAA AGCCTCCAAA CTCATGGAAA TCGAAACCGT TCTGGAGGAG GCGCGCAAGG CCAAGGCTGC GGGTGCCACG CGCTATTGCA TGGGGGCAGC TTGGCGCAGC CCCAAGGATC GCGACATGCC CGCCCTCACC CATATGATCG AAAGCGTGAA GGCAATGGGC CTGGAAACCT GCATGACGCT GGGGATGCTG GATAGCGACA AGGCGGAAAA ACTCGCCGAT GCAGGCCTCG ATTATTACAA TCACAATATC GATACGTCCG AGCGTTTTTA TCCCGCAGTC ATCACCACGC GCAGTTTTGA AGATCGGCTC GATACGCTTG CAAATGTGCG CAATGCGGGC ATCAAGGTTT GCAGCGGCGG TATTCTGGGG CTTGGCGAAG AGGCCGAAGA TCGCATCGAC ATGCTGGTAA CACTGGCCAA CCTGCCGGAA CCGCCGGAGA GCGTGCCGAT CAATATGCTG ATCCCCATGC CGGGCACACG GCTGGCAAAG GCCGCGCCAG TCGATCCGCT TGAATTTGTC CGTGTGGTCG CGCTGGCGCG CATTCTCATG CCGAAATCCC ATGTGCGCCT GACGGCTGGC CGTACCGCCA TGAGCGACGA GATGCAGGCG CTCTGCTTCT TTGCCGGAGC CAATTCGCTA TTTATGGGCG ACACCTTGCT CACAGCCGCC AACCCCGGCG ACGACCGCGA TTCCAGCCTG CTGCGCCGCC TCGGCATACA GGCGGAAACG GAGAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA
Details for BrabA.00121.a.A4.PW27965
PURIFICATION DATe: 2/8/2010
CONCENTRATION: 28.46mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MRWSAEEARA IYNLPFNDLL FRAHGLHREN FDPNRIQLSK LLNIKTGGCP EDCGYCSQSA SAENGLKASK LMEIETVLEE ARKAKAAGAT RYCMGAAWRS PKDRDMPALT HMIESVKAMG LETCMTLGML DSDKAEKLAD AGLDYYNHNI DTSERFYPAV ITTRSFEDRL DTLANVRNAG IKVCSGGILG LGEEAEDRID MLVTLANLPE PPESVPINML IPMPGTRLAK AAPVDPLEFV RVVALARILM PKSHVRLTAG RTAMSDEMQA LCFFAGANSL FMGDTLLTAA NPGDDRDSSL LRRLGIQAET EQPA
Validated NT Sequence
ggtaccctgg aagctcagac ccagggtcct ggttcgatgc gttggagcgc agaagaagcg cgggcaatct ataatctgcc gttcaatgat ctgctctttc gcgcccacgg cctgcaccgc gagaattttg atccgaaccg catccagctc agcaagcttc tcaacatcaa gacgggtgga tgcccggaag attgcggtta ttgcagccag tctgcttctg cggaaaatgg cctgaaagcc tccaaactca tggaaatcga aaccgttctg gaggaggcgc gcaaggccaa ggctgcgggt gccacgcgct attgcatggg ggcagcttgg cgcagcccca aggatcgcga catgcccgcc ctcacccata tgatcgaaag cgtgaaggca atgggcctgg aaacctgcat gacgctgggg atgctggata gcgacaaggc ggaaaaactc gccgatgcag gcctcgatta ttacaatcac aatatcgata cgtccgagcg tttttatccc gcagtcatca ccacgcgcag ttttgaagat cggctcgata cgcttgcaaa tgtgcgcaat gcgggcatca aggtttgcag cggcggtatt ctggggcttg gcgaagaggc cgaagatcgc atcgacatgc tggtaacact ggccaacctg ccggaaccgc cggagagcgt gccgatcaat atgctgatcc ccatgccggg cacacggctg gcaaaggccg cgccagtcga tccgcttgaa tttgtccgtg tggtcgcgct ggcgcgcatt ctcatgccga aatcccatgt gcgcctgacg gctggccgta ccgccatgag cgacgagatg caggcgctct gcttctttgc cggagccaat tcgctattta tgggcgacac cttgctcaca gccgccaacc ccggcgacga ccgcgattcc agcctgctgc gccgcctcgg catacaggcg gaaacggagc agcctgcgta ataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRWSAEEAR AIYNLPFNDL LFRAHGLHRE NFDPNRIQLS KLLNIKTGGC PEDCGYCSQS ASAENGLKAS KLMEIETVLE EARKAKAAGA TRYCMGAAWR SPKDRDMPAL THMIESVKAM GLETCMTLGM LDSDKAEKLA DAGLDYYNHN IDTSERFYPA VITTRSFEDR LDTLANVRNA GIKVCSGGIL GLGEEAEDRI DMLVTLANLP EPPESVPINM LIPMPGTRLA KAAPVDPLEF VRVVALARIL MPKSHVRLTA GRTAMSDEMQ ALCFFAGANS LFMGDTLLTA ANPGDDRDSS LLRRLGIQAE TEQPA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGTTGGAGC GCAGAAGAAG CGCGGGCAAT CTATAATCTG CCGTTCAATG ATCTGCTCTT TCGCGCCCAC GGCCTGCACC GCGAGAATTT TGATCCGAAC CGCATCCAGC TCAGCAAGCT TCTCAACATC AAGACGGGTG GATGCCCGGA AGATTGCGGT TATTGCAGCC AGTCTGCTTC TGCGGAAAAT GGCCTGAAAG CCTCCAAACT CATGGAAATC GAAACCGTTC TGGAGGAGGC GCGCAAGGCC AAGGCTGCGG GTGCCACGCG CTATTGCATG GGGGCAGCTT GGCGCAGCCC CAAGGATCGC GACATGCCCG CCCTCACCCA TATGATCGAA AGCGTGAAGG CAATGGGCCT GGAAACCTGC ATGACGCTGG GGATGCTGGA TAGCGACAAG GCGGAAAAAC TCGCCGATGC AGGCCTCGAT TATTACAATC ACAATATCGA TACGTCCGAG CGTTTTTATC CCGCAGTCAT CACCACGCGC AGTTTTGAAG ATCGGCTCGA TACGCTTGCA AATGTGCGCA ATGCGGGCAT CAAGGTTTGC AGCGGCGGTA TTCTGGGGCT TGGCGAAGAG GCCGAAGATC GCATCGACAT GCTGGTAACA CTGGCCAACC TGCCGGAACC GCCGGAGAGC GTGCCGATCA ATATGCTGAT CCCCATGCCG GGCACACGGC TGGCAAAGGC CGCGCCAGTC GATCCGCTTG AATTTGTCCG TGTGGTCGCG CTGGCGCGCA TTCTCATGCC GAAATCCCAT GTGCGCCTGA CGGCTGGCCG TACCGCCATG AGCGACGAGA TGCAGGCGCT CTGCTTCTTT GCCGGAGCCA ATTCGCTATT TATGGGCGAC ACCTTGCTCA CAGCCGCCAA CCCCGGCGAC GACCGCGATT CCAGCCTGCT GCGCCGCCTC GGCATACAGG CGGAAACGGA GCAGCCTGCG AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA
Details for BrabA.00121.a.A6.PW27967
PURIFICATION DATe: 2/8/2010
CONCENTRATION: 28.37mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMRWSAEE ARAIYNLPFN DLLFRAHGLH RENFDPNRIQ LSKLLNIKTG GCPEDCGYCS QSASAENGLK ASKLMEIETV LEEARKAKAA GATRYCMGAA WRSPKDRDMP ALTHMIESVK AMGLETCMTL GMLDSDKAEK LADAGLDYYN HNIDTSERFY PAVITTRSFE DRLDTLANVR NAGIKVCSGG ILGLGEEAED RIDMLVTLAN LPEPPESVPI NMLIPMPGTR LAKAAPVDPL EFVRVVALAR ILMPKSHVRL TAGRTAMSDE MQALCFFAGA NSLFMGDTLL TAANPGDDRD SSLLRRLGIQ AETE
Validated NT Sequence
ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgcgttg gagcgcagaa gaagcgcggg caatctataa tctgccgttc aatgatctgc tctttcgcgc ccacggcctg caccgcgaga attttgatcc gaaccgcatc cagctcagca agcttctcaa catcaagacg ggtggatgcc cggaagattg cggttattgc agccagtctg cttctgcgga aaatggcctg aaagcctcca aactcatgga aatcgaaacc gttctggagg aggcgcgcaa ggccaaggct gcgggtgcca cgcgctattg catgggggca gcttggcgca gccccaagga tcgcgacatg cccgccctca cccatatgat cgaaagcgtg aaggcaatgg gcctggaaac ctgcatgacg ctggggatgc tggatagcga caaggcggaa aaactcgccg atgcaggcct cgattattac aatcacaata tcgatacgtc cgagcgtttt tatcccgcag tcatcaccac gcgcagtttt gaagatcggc tcgatacgct tgcaaatgtg cgcaatgcgg gcatcaaggt ttgcagcggc ggtattctgg ggcttggcga agaggccgaa gatcgcatcg acatgctggt aacactggcc aacctgccgg aaccgccgga gagcgtgccg atcaatatgc tgatccccat gccgggcaca cggctggcaa aggccgcgcc agtcgatccg cttgaatttg tccgtgtggt cgcgctggcg cgcattctca tgccgaaatc ccatgtgcgc ctgacggctg gccgtaccgc catgagcgac gagatgcagg cgctctgctt ctttgccgga gccaattcgc tatttatggg cgacaccttg ctcacagccg ccaaccccgg cgacgaccgc gattccagcc tgctgcgccg cctcggcata caggcggaaa cggagtaata a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRWSAEEAR AIYNLPFNDL LFRAHGLHRE NFDPNRIQLS KLLNIKTGGC PEDCGYCSQS ASAENGLKAS KLMEIETVLE EARKAKAAGA TRYCMGAAWR SPKDRDMPAL THMIESVKAM GLETCMTLGM LDSDKAEKLA DAGLDYYNHN IDTSERFYPA VITTRSFEDR LDTLANVRNA GIKVCSGGIL GLGEEAEDRI DMLVTLANLP EPPESVPINM LIPMPGTRLA KAAPVDPLEF VRVVALARIL MPKSHVRLTA GRTAMSDEMQ ALCFFAGANS LFMGDTLLTA ANPGDDRDSS LLRRLGIQAE TE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGTTGGAGC GCAGAAGAAG CGCGGGCAAT CTATAATCTG CCGTTCAATG ATCTGCTCTT TCGCGCCCAC GGCCTGCACC GCGAGAATTT TGATCCGAAC CGCATCCAGC TCAGCAAGCT TCTCAACATC AAGACGGGTG GATGCCCGGA AGATTGCGGT TATTGCAGCC AGTCTGCTTC TGCGGAAAAT GGCCTGAAAG CCTCCAAACT CATGGAAATC GAAACCGTTC TGGAGGAGGC GCGCAAGGCC AAGGCTGCGG GTGCCACGCG CTATTGCATG GGGGCAGCTT GGCGCAGCCC CAAGGATCGC GACATGCCCG CCCTCACCCA TATGATCGAA AGCGTGAAGG CAATGGGCCT GGAAACCTGC ATGACGCTGG GGATGCTGGA TAGCGACAAG GCGGAAAAAC TCGCCGATGC AGGCCTCGAT TATTACAATC ACAATATCGA TACGTCCGAG CGTTTTTATC CCGCAGTCAT CACCACGCGC AGTTTTGAAG ATCGGCTCGA TACGCTTGCA AATGTGCGCA ATGCGGGCAT CAAGGTTTGC AGCGGCGGTA TTCTGGGGCT TGGCGAAGAG GCCGAAGATC GCATCGACAT GCTGGTAACA CTGGCCAACC TGCCGGAACC GCCGGAGAGC GTGCCGATCA ATATGCTGAT CCCCATGCCG GGCACACGGC TGGCAAAGGC CGCGCCAGTC GATCCGCTTG AATTTGTCCG TGTGGTCGCG CTGGCGCGCA TTCTCATGCC GAAATCCCAT GTGCGCCTGA CGGCTGGCCG TACCGCCATG AGCGACGAGA TGCAGGCGCT CTGCTTCTTT GCCGGAGCCA ATTCGCTATT TATGGGCGAC ACCTTGCTCA CAGCCGCCAA CCCCGGCGAC GACCGCGATT CCAGCCTGCT GCGCCGCCTC GGCATACAGG CGGAAACGGA GAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA