SSGCID: Target Page

BrabA.00119.a

D-alanine--D-alanine ligase/VANA/B/C:D-alanine--D-alanine ligase

CENTER ID:	BrabA.00119.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.00119.a.A1.GE32517	Full length( BrabA.00119.a )		1	308

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.00119.a.A1.PS01143	Full length( BrabA.00119.a )		1	308

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.1504
RefSeq:	YP_414819.1
UniProt:	Q2YLY8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTGKHVAVLM GGFSSERSVS LSSGVACATT LEECGYRVTR IDVDRNVASI LVELKPDVVF NALHGPFGED GAIQGVLEYL QIPYTHSGVL ASALAMDKDR AKKVAAAAGV VVAPSRLMNR FDIGSQHPMK PPYVVKPVRE GSSFGVVIVK EDQPHPPQVI GSADWKYGDE VMVEGYIAGR ELTCAVMGDR AMDVCEIMPV GYQFYDYDSK YVAGASTHVC PAKILPNIYQ KIQTMALTAH RAIGCRGVSR SDFRFDDRFS EEGEVVWLEI NTQPGMTPTS LVPDIAKAAG ISFAELLSWM MEDASCLR

NT Sequence

ATGACGGGAA AACACGTCGC CGTTTTGATG GGTGGTTTTT CTTCGGAACG CTCCGTCAGC CTTTCCTCCG GTGTGGCCTG TGCAACGACG CTTGAAGAAT GCGGCTATCG CGTCACGCGC ATTGATGTGG ATCGCAATGT TGCGAGCATC CTGGTCGAGT TGAAACCCGA TGTTGTGTTC AACGCCTTGC ACGGCCCCTT CGGCGAAGAT GGCGCCATTC AGGGCGTGCT TGAATATCTC CAGATTCCCT ACACACATTC CGGCGTGCTG GCTTCCGCGC TTGCGATGGA CAAGGATCGC GCCAAGAAGG TGGCGGCAGC GGCGGGCGTC GTGGTTGCGC CGTCACGCCT GATGAACCGT TTCGACATTG GTTCGCAGCA TCCGATGAAG CCGCCTTACG TGGTGAAGCC GGTGCGTGAA GGGTCGAGCT TCGGTGTGGT GATCGTCAAG GAAGATCAGC CGCATCCGCC GCAGGTCATC GGTTCGGCCG ATTGGAAATA TGGTGATGAG GTCATGGTCG AAGGCTATAT TGCCGGGCGT GAGCTGACCT GTGCCGTGAT GGGGGATCGC GCCATGGATG TGTGCGAGAT CATGCCGGTC GGTTATCAGT TCTACGACTA TGATTCAAAA TATGTTGCAG GTGCATCAAC TCACGTGTGC CCGGCAAAAA TTTTACCAAA TATTTACCAA AAAATACAAA CAATGGCCCT TACGGCGCAT CGGGCAATCG GGTGTCGGGG CGTAAGCCGG TCGGACTTCC GTTTCGATGA TCGTTTTTCT GAAGAAGGCG AAGTTGTCTG GCTGGAAATC AATACCCAGC CGGGGATGAC TCCTACCTCG CTCGTGCCTG ATATCGCCAA AGCGGCGGGT ATCTCTTTCG CTGAGTTGTT GAGTTGGATG ATGGAGGACG CGTCTTGTTT GCGT

Details for BrabA.00119.a.A1.GE32517

HARVESTED ON:	5/17/2011
SEQUENCED ON:	5/23/2011
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMTGKHVA VLMGGFSSER SVSLSSGVAC ATTLEECGYR VTRIDVDRNV ASILVELKPD VVFNALHGPF GEDGAIQGVL EYLQIPYTHS GVLASALAMD KDRAKKVAAA AGVVVAPSRL MNRFDIGSQH PMKPPYVVKP VREGSSFGVV IVKEDQPHPP QVIGSADWKY GDEVMVEGYI AGRELTCAVM GDRAMDVCEI MPVGYQFYDY DSKYVAGAST HVCPAKILPN IYQKIQTMAL TAHRAIGCRG VSRSDFRFDD RFSEEGEVVW LEINTQPGMT PTSLVPDIAK AAGISFAELL SWMMEDASCL R

Validated NT Sequence

gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgacgggaa aacacgtcgc cgttttgatg ggtggttttt cttcggaacg ctccgtcagc ctttcctccg gtgtggcctg tgcaacgacg cttgaagaat gcggctatcg cgtcacgcgc attgatgtgg atcgcaatgt tgcgagcatc ctggtcgagt tgaaacccga tgttgtgttc aacgccttgc acggcccctt cggcgaagat ggcgccattc agggcgtgct tgaatatctc cagattccct acacacattc cggcgtgctg gcttccgcgc ttgcgatgga caaggatcgc gccaagaagg tggcggcagc ggcgggcgtc gtggttgcgc cgtcacgcct gatgaaccgt ttcgacattg gttcgcagca tccgatgaag ccgccttacg tggtgaagcc ggtgcgtgaa gggtcgagct tcggtgtggt gatcgtcaag gaagatcagc cgcatccgcc gcaggtcatc ggttcggccg attggaaata tggtgatgag gtcatggtcg aaggctatat tgccgggcgt gagctgacct gtgccgtgat gggggatcgc gccatggatg tgtgcgagat catgccggtc ggttatcagt tctacgacta tgattcaaaa tatgttgcag gtgcatcaac tcacgtgtgc ccggcaaaaa ttttaccaaa tatttaccaa aaaatacaaa caatggccct tacggcgcat cgggcaatcg ggtgtcgggg cgtaagccgg tcggacttcc gtttcgatga tcgtttttct gaagaaggcg aagttgtctg gctggaaatc aatacccagc cggggatgac tcctacctcg ctcgtgcctg atatcgccaa agcggcgggt atctctttcg ctgagttgtt gagttggatg atggaggacg cgtcttgttt gcgttaataa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTGKHVAVL MGGFSSERSV SLSSGVACAT TLEECGYRVT RIDVDRNVAS ILVELKPDVV FNALHGPFGE DGAIQGVLEY LQIPYTHSGV LASALAMDKD RAKKVAAAAG VVVAPSRLMN RFDIGSQHPM KPPYVVKPVR EGSSFGVVIV KEDQPHPPQV IGSADWKYGD EVMVEGYIAG RELTCAVMGD RAMDVCEIMP VGYQFYDYDS KYVAGASTHV CPAKILPNIY QKIQTMALTA HRAIGCRGVS RSDFRFDDRF SEEGEVVWLE INTQPGMTPT SLVPDIAKAA GISFAELLSW MMEDASCLR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgggaaaa cacgtcgccg ttttgatggg tggtttttct tcggaacgct ccgtcagcct ttcctccggt gtggcctgtg caacgacgct tgaagaatgc ggctatcgcg tcacgcgcat tgatgtggat cgcaatgttg cgagcatcct ggtcgagttg aaacccgatg ttgtgttcaa cgccttgcac ggccccttcg gcgaagatgg cgccattcag ggcgtgcttg aatatctcca gattccctac acacattccg gcgtgctggc ttccgcgctt gcgatggaca aggatcgcgc caagaaggtg gcggcagcgg cgggcgtcgt ggttgcgccg tcacgcctga tgaaccgttt cgacattggt tcgcagcatc cgatgaagcc gccttacgtg gtgaagccgg tgcgtgaagg gtcgagcttc ggtgtggtga tcgtcaagga agatcagccg catccgccgc aggtcatcgg ttcggccgat tggaaatatg gtgatgaggt catggtcgaa ggctatattg ccgggcgtga gctgacctgt gccgtgatgg gggatcgcgc catggatgtg tgcgagatca tgccggtcgg ttatcagttc tacgactatg attcaaaata tgttgcaggt gcatcaactc acgtgtgccc ggcaaaaatt ttaccaaata tttaccaaaa aatacaaaca atggccctta cggcgcatcg ggcaatcggg tgtcggggcg taagccggtc ggacttccgt ttcgatgatc gtttttctga agaaggcgaa gttgtctggc tggaaatcaa tacccagccg gggatgactc ctacctcgct cgtgcctgat atcgccaaag cggcgggtat ctctttcgct gagttgttga gttggatgat ggaggacgcg tcttgtttgc gttaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for BrabA.00119.a.A1.PS01143

PURIFICATION DATe:	6/29/2011
CONCENTRATION:	34.4mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGGGAAAA CACGTCGCCG TTTTGATGGG TGGTTTTTCT TCGGAACGCT CCGTCAGCCT TTCCTCCGGT GTGGCCTGTG CAACGACGCT TGAAGAATGC GGCTATCGCG TCACGCGCAT TGATGTGGAT CGCAATGTTG CGAGCATCCT GGTCGAGTTG AAACCCGATG TTGTGTTCAA CGCCTTGCAC GGCCCCTTCG GCGAAGATGG CGCCATTCAG GGCGTGCTTG AATATCTCCA GATTCCCTAC ACACATTCCG GCGTGCTGGC TTCCGCGCTT GCGATGGACA AGGATCGCGC CAAGAAGGTG GCGGCAGCGG CGGGCGTCGT GGTTGCGCCG TCACGCCTGA TGAACCGTTT CGACATTGGT TCGCAGCATC CGATGAAGCC GCCTTACGTG GTGAAGCCGG TGCGTGAAGG GTCGAGCTTC GGTGTGGTGA TCGTCAAGGA AGATCAGCCG CATCCGCCGC AGGTCATCGG TTCGGCCGAT TGGAAATATG GTGATGAGGT CATGGTCGAA GGCTATATTG CCGGGCGTGA GCTGACCTGT GCCGTGATGG GGGATCGCGC CATGGATGTG TGCGAGATCA TGCCGGTCGG TTATCAGTTC TACGACTATG ATTCAAAATA TGTTGCAGGT GCATCAACTC ACGTGTGCCC GGCAAAAATT TTACCAAATA TTTACCAAAA AATACAAACA ATGGCCCTTA CGGCGCATCG GGCAATCGGG TGTCGGGGCG TAAGCCGGTC GGACTTCCGT TTCGATGATC GTTTTTCTGA AGAAGGCGAA GTTGTCTGGC TGGAAATCAA TACCCAGCCG GGGATGACTC CTACCTCGCT CGTGCCTGAT ATCGCCAAAG CGGCGGGTAT CTCTTTCGCT GAGTTGTTGA GTTGGATGAT GGAGGACGCG TCTTGTTTGC GTTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA