BrabA.00113.b
3-oxoacyl-(acyl carrier protein) synthase II
| CENTER ID: | BrabA.00113.b |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00113.b.B1.GE27576 | Full length( BrabA.00113.b ) | 1 | 420 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00113.b.B1.PS00386 | Full length( BrabA.00113.b ) | 1 | 420 |
Structures
3KZU
DEPOSITED: 12/8/2009
DETERMINATION: XRay
CLONE: BrabA.00113.b.B1.GE27576
PROTEIN: BrabA.00113.b.B1.PS00386
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126985 |
| PATRIC ID: | fig|359391.4.peg.507 |
| RefSeq: | YP_413946.1 |
| UniProt: | Q2YMJ5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRRVVITGLG LVSPLASGVE ETWKRLLAGE SGARRVTEFE VDDLACQIAC RIPVGDGTNG TFNPDLHMDP KEQRKVDPFI VYAVGAADQA LDDAGWHPEN DEDQVRTGVL IGSGIGGIEG IVEAGYTLRD KGPRRISPFF IPGRLINLAS GHVSIKHKLR GPNHSVVTAC ATGTHAIGDA ARLIAFGDAD VMVAGGTESP VSRISLAGFA ACKALSTERN DDPTAASRPY DEDRDGFVMG EGAGIVVLEE LEHALARGAK IYAEVIGYGM SGDAFHITAP TESGEGAQRC MVAALKRAGI VPDEIDYINA HGTSTMADTI ELGAVERVVG EAAAKISMSS TKSSIGHLLG AAGAAEAVFS TLAIRDNIAP ATLNLDNPAA QTRIDLVPHK PRERKIDVAL SNSFGFGGTN ASLVLRRYTA
NT Sequence
ATGAGGCGTG TCGTCATCAC CGGTCTCGGT CTGGTGTCAC CGCTCGCAAG CGGTGTGGAA GAGACTTGGA AGCGGCTTCT TGCCGGTGAA AGCGGGGCTC GCCGCGTTAC GGAATTCGAG GTGGACGATC TGGCCTGCCA GATTGCCTGC CGCATTCCCG TTGGCGATGG CACGAACGGT ACGTTCAATC CCGACCTGCA TATGGACCCG AAGGAACAGC GCAAGGTCGA TCCTTTCATC GTTTATGCCG TCGGTGCAGC CGATCAGGCT CTGGATGATG CCGGCTGGCA CCCGGAAAAT GATGAGGATC AGGTCCGCAC CGGCGTTCTT ATCGGTTCGG GCATCGGCGG CATCGAAGGC ATTGTCGAGG CGGGCTACAC GCTGCGCGAC AAGGGCCCGC GCCGTATTTC GCCCTTCTTC ATTCCGGGCC GTCTCATCAA TCTGGCCTCC GGCCATGTGT CGATCAAGCA CAAGCTGCGC GGGCCGAACC ACTCGGTCGT CACGGCCTGT GCAACCGGCA CGCACGCTAT TGGCGATGCC GCCCGCCTCA TTGCTTTCGG CGATGCGGAT GTGATGGTTG CCGGTGGCAC GGAATCGCCG GTGAGCCGCA TTTCGCTTGC TGGCTTTGCC GCCTGCAAGG CGCTTTCCAC CGAGCGCAAT GACGATCCGA CCGCCGCTTC ACGTCCTTAT GACGAGGATC GTGACGGCTT CGTCATGGGC GAAGGCGCTG GCATTGTGGT GCTTGAAGAG CTGGAACACG CTTTGGCGCG CGGTGCGAAG ATTTATGCCG AAGTCATCGG CTATGGCATG TCGGGCGATG CCTTCCATAT CACGGCCCCG ACCGAGAGCG GTGAGGGCGC ACAGCGCTGC ATGGTGGCGG CTCTCAAGCG TGCCGGGATC GTGCCGGACG AGATCGACTA TATCAATGCG CATGGCACGT CCACCATGGC CGATACGATC GAGCTTGGCG CGGTGGAGCG CGTGGTGGGT GAGGCGGCTG CGAAGATTTC GATGTCTTCC ACCAAATCAT CCATCGGCCA CCTGTTGGGT GCGGCAGGGG CTGCCGAAGC GGTCTTCTCC ACGCTGGCCA TTCGCGACAA TATCGCGCCT GCCACACTCA ATCTGGACAA TCCGGCGGCG CAGACCAGGA TCGATCTCGT GCCGCACAAG CCGCGCGAAC GCAAGATCGA TGTGGCGCTG TCGAATTCGT TCGGTTTCGG TGGCACCAAT GCATCGCTGG TGCTGCGCCG CTACACGGCC
Details for BrabA.00113.b.B1.GE27576
| HARVESTED ON: | 8/17/2009 |
| SEQUENCED ON: | 3/21/2012 |
| EXPECTED MW: | 45kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMR RVVITGLGLV SPLASGVEET WKRLLAGESG ARRVTEFEVD DLACQIACRI PVGDGTNGTF NPDLHMDPKE QRKVDPFIVY AVGAADQALD DAGWHPENDE DQVRTGVLIG SGIGGIEGIV EAGYTLRDKG PRRISPFFIP GRLINLASGH VSIKHKLRGP NHSVVTACAT GTHAIGDAAR LIAFGDADVM VAGGTESPVS RISLAGFAAC KALSTERNDD PTAASRPYDE DRDGFVMGEG AGIVVLEELE HALARGAKIY AEVIGYGMSG DAFHITAPTE SGEGAQRCMV AALKRAGIVP DEIDYINAHG TSTMADTIEL GAVERVVGEA AAKISMSSTK SSIGHLLGAA GAAEAVFSTL AIRDNIAPAT LNLDNPAAQT RIDLVPHKPR ERKIDVALSN SFGFGGTNAS LVLRRYTA
Validated NT Sequence
atggctcacc accaccacca ccatatgagg cgtgtcgtca tcaccggtct cggtctggtg tcaccgctcg caagcggtgt ggaagagact tggaagcggc ttcttgccgg tgaaagcggg gctcgccgcg ttacggaatt cgaggtggac gatctggcct gccagattgc ctgccgcatt cccgttggcg atggcacgaa cggtacgttc aatcccgacc tgcatatgga cccgaaggaa cagcgcaagg tcgatccttt catcgtttat gccgtcggtg cagccgatca ggctctggat gatgccggct ggcacccgga aaatgatgag gatcaggtcc gcaccggcgt tcttatcggt tcgggcatcg gcggcatcga aggcattgtc gaggcgggct acacgctgcg cgacaagggc ccgcgccgta tttcgccctt cttcattccg ggccgtctca tcaatctggc ctccggccat gtgtcgatca agcacaagct gcgcgggccg aaccactcgg tcgtcacggc ctgtgcaacc ggcacgcacg ctattggcga tgccgcccgc ctcattgctt tcggcgatgc ggatgtgatg gttgccggtg gcacggaatc gccggtgagc cgcatttcgc ttgctggctt tgccgcctgc aaggcgcttt ccaccgagcg caatgacgat ccgaccgccg cttcacgtcc ttatgacgag gatcgtgacg gcttcgtcat gggcgaaggc gctggcattg tggtgcttga agagctggaa cacgctttgg cgcgcggtgc gaagatttat gccgaagtca tcggctatgg catgtcgggc gatgccttcc atatcacggc cccgaccgag agcggtgagg gcgcacagcg ctgcatggtg gcggctctca agcgtgccgg gatcgtgccg gacgagatcg actatatcaa tgcgcatggc acgtccacca tggccgatac gatcgagctt ggcgcggtgg agcgcgtggt gggtgaggcg gctgcgaaga tttcgatgtc ttccaccaaa tcatccatcg gccacctgtt gggtgcggca ggggctgccg aagcggtctt ctccacgctg gccattcgcg acaatatcgc gcctgccaca ctcaatctgg acaatccggc ggcgcagacc aggatcgatc tcgtgccgca caagccgcgc gaacgcaaga tcgatgtggc gctgtcgaat tcgttcggtt tcggtggcac caatgcatcg ctggtgctgc gccgctacac ggcctaagtg agtaagat
Expected Protein Sequence
MAHHHHHHMR RVVITGLGLV SPLASGVEET WKRLLAGESG ARRVTEFEVD DLACQIACRI PVGDGTNGTF NPDLHMDPKE QRKVDPFIVY AVGAADQALD DAGWHPENDE DQVRTGVLIG SGIGGIEGIV EAGYTLRDKG PRRISPFFIP GRLINLASGH VSIKHKLRGP NHSVVTACAT GTHAIGDAAR LIAFGDADVM VAGGTESPVS RISLAGFAAC KALSTERNDD PTAASRPYDE DRDGFVMGEG AGIVVLEELE HALARGAKIY AEVIGYGMSG DAFHITAPTE SGEGAQRCMV AALKRAGIVP DEIDYINAHG TSTMADTIEL GAVERVVGEA AAKISMSSTK SSIGHLLGAA GAAEAVFSTL AIRDNIAPAT LNLDNPAAQT RIDLVPHKPR ERKIDVALSN SFGFGGTNAS LVLRRYTA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaggc gtgtcgtcat caccggtctc ggtctggtgt caccgctcgc aagcggtgtg gaagagactt ggaagcggct tcttgccggt gaaagcgggg ctcgccgcgt tacggaattc gaggtggacg atctggcctg ccagattgcc tgccgcattc ccgttggcga tggcacgaac ggtacgttca atcccgacct gcatatggac ccgaaggaac agcgcaaggt cgatcctttc atcgtttatg ccgtcggtgc agccgatcag gctctggatg atgccggctg gcacccggaa aatgatgagg atcaggtccg caccggcgtt cttatcggtt cgggcatcgg cggcatcgaa ggcattgtcg aggcgggcta cacgctgcgc gacaagggcc cgcgccgtat ttcgcccttc ttcattccgg gccgtctcat caatctggcc tccggccatg tgtcgatcaa gcacaagctg cgcgggccga accactcggt cgtcacggcc tgtgcaaccg gcacgcacgc tattggcgat gccgcccgcc tcattgcttt cggcgatgcg gatgtgatgg ttgccggtgg cacggaatcg ccggtgagcc gcatttcgct tgctggcttt gccgcctgca aggcgctttc caccgagcgc aatgacgatc cgaccgccgc ttcacgtcct tatgacgagg atcgtgacgg cttcgtcatg ggcgaaggcg ctggcattgt ggtgcttgaa gagctggaac acgctttggc gcgcggtgcg aagatttatg ccgaagtcat cggctatggc atgtcgggcg atgccttcca tatcacggcc ccgaccgaga gcggtgaggg cgcacagcgc tgcatggtgg cggctctcaa gcgtgccggg atcgtgccgg acgagatcga ctatatcaat gcgcatggca cgtccaccat ggccgatacg atcgagcttg gcgcggtgga gcgcgtggtg ggtgaggcgg ctgcgaagat ttcgatgtct tccaccaaat catccatcgg ccacctgttg ggtgcggcag gggctgccga agcggtcttc tccacgctgg ccattcgcga caatatcgcg cctgccacac tcaatctgga caatccggcg gcgcagacca ggatcgatct cgtgccgcac aagccgcgcg aacgcaagat cgatgtggcg ctgtcgaatt cgttcggttt cggtggcacc aatgcatcgc tggtgctgcg ccgctacacg gcctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for BrabA.00113.b.B1.PS00386
| PURIFICATION DATe: | 10/20/2009 |
| CONCENTRATION: | 50.99mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMR RVVITGLGLV SPLASGVEET WKRLLAGESG ARRVTEFEVD DLACQIACRI PVGDGTNGTF NPDLHMDPKE QRKVDPFIVY AVGAADQALD DAGWHPENDE DQVRTGVLIG SGIGGIEGIV EAGYTLRDKG PRRISPFFIP GRLINLASGH VSIKHKLRGP NHSVVTACAT GTHAIGDAAR LIAFGDADVM VAGGTESPVS RISLAGFAAC KALSTERNDD PTAASRPYDE DRDGFVMGEG AGIVVLEELE HALARGAKIY AEVIGYGMSG DAFHITAPTE SGEGAQRCMV AALKRAGIVP DEIDYINAHG TSTMADTIEL GAVERVVGEA AAKISMSSTK SSIGHLLGAA GAAEAVFSTL AIRDNIAPAT LNLDNPAAQT RIDLVPHKPR ERKIDVALSN SFGFGGTNAS LVLRRYTA
Validated NT Sequence
atggctcacc accaccacca ccatatgagg cgtgtcgtca tcaccggtct cggtctggtg tcaccgctcg caagcggtgt ggaagagact tggaagcggc ttcttgccgg tgaaagcggg gctcgccgcg ttacggaatt cgaggtggac gatctggcct gccagattgc ctgccgcatt cccgttggcg atggcacgaa cggtacgttc aatcccgacc tgcatatgga cccgaaggaa cagcgcaagg tcgatccttt catcgtttat gccgtcggtg cagccgatca ggctctggat gatgccggct ggcacccgga aaatgatgag gatcaggtcc gcaccggcgt tcttatcggt tcgggcatcg gcggcatcga aggcattgtc gaggcgggct acacgctgcg cgacaagggc ccgcgccgta tttcgccctt cttcattccg ggccgtctca tcaatctggc ctccggccat gtgtcgatca agcacaagct gcgcgggccg aaccactcgg tcgtcacggc ctgtgcaacc ggcacgcacg ctattggcga tgccgcccgc ctcattgctt tcggcgatgc ggatgtgatg gttgccggtg gcacggaatc gccggtgagc cgcatttcgc ttgctggctt tgccgcctgc aaggcgcttt ccaccgagcg caatgacgat ccgaccgccg cttcacgtcc ttatgacgag gatcgtgacg gcttcgtcat gggcgaaggc gctggcattg tggtgcttga agagctggaa cacgctttgg cgcgcggtgc gaagatttat gccgaagtca tcggctatgg catgtcgggc gatgccttcc atatcacggc cccgaccgag agcggtgagg gcgcacagcg ctgcatggtg gcggctctca agcgtgccgg gatcgtgccg gacgagatcg actatatcaa tgcgcatggc acgtccacca tggccgatac gatcgagctt ggcgcggtgg agcgcgtggt gggtgaggcg gctgcgaaga tttcgatgtc ttccaccaaa tcatccatcg gccacctgtt gggtgcggca ggggctgccg aagcggtctt ctccacgctg gccattcgcg acaatatcgc gcctgccaca ctcaatctgg acaatccggc ggcgcagacc aggatcgatc tcgtgccgca caagccgcgc gaacgcaaga tcgatgtggc gctgtcgaat tcgttcggtt tcggtggcac caatgcatcg ctggtgctgc gccgctacac ggcctaagtg agtaagat
Expressed Protein Sequence
MAHHHHHHMR RVVITGLGLV SPLASGVEET WKRLLAGESG ARRVTEFEVD DLACQIACRI PVGDGTNGTF NPDLHMDPKE QRKVDPFIVY AVGAADQALD DAGWHPENDE DQVRTGVLIG SGIGGIEGIV EAGYTLRDKG PRRISPFFIP GRLINLASGH VSIKHKLRGP NHSVVTACAT GTHAIGDAAR LIAFGDADVM VAGGTESPVS RISLAGFAAC KALSTERNDD PTAASRPYDE DRDGFVMGEG AGIVVLEELE HALARGAKIY AEVIGYGMSG DAFHITAPTE SGEGAQRCMV AALKRAGIVP DEIDYINAHG TSTMADTIEL GAVERVVGEA AAKISMSSTK SSIGHLLGAA GAAEAVFSTL AIRDNIAPAT LNLDNPAAQT RIDLVPHKPR ERKIDVALSN SFGFGGTNAS LVLRRYTA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGGC GTGTCGTCAT CACCGGTCTC GGTCTGGTGT CACCGCTCGC AAGCGGTGTG GAAGAGACTT GGAAGCGGCT TCTTGCCGGT GAAAGCGGGG CTCGCCGCGT TACGGAATTC GAGGTGGACG ATCTGGCCTG CCAGATTGCC TGCCGCATTC CCGTTGGCGA TGGCACGAAC GGTACGTTCA ATCCCGACCT GCATATGGAC CCGAAGGAAC AGCGCAAGGT CGATCCTTTC ATCGTTTATG CCGTCGGTGC AGCCGATCAG GCTCTGGATG ATGCCGGCTG GCACCCGGAA AATGATGAGG ATCAGGTCCG CACCGGCGTT CTTATCGGTT CGGGCATCGG CGGCATCGAA GGCATTGTCG AGGCGGGCTA CACGCTGCGC GACAAGGGCC CGCGCCGTAT TTCGCCCTTC TTCATTCCGG GCCGTCTCAT CAATCTGGCC TCCGGCCATG TGTCGATCAA GCACAAGCTG CGCGGGCCGA ACCACTCGGT CGTCACGGCC TGTGCAACCG GCACGCACGC TATTGGCGAT GCCGCCCGCC TCATTGCTTT CGGCGATGCG GATGTGATGG TTGCCGGTGG CACGGAATCG CCGGTGAGCC GCATTTCGCT TGCTGGCTTT GCCGCCTGCA AGGCGCTTTC CACCGAGCGC AATGACGATC CGACCGCCGC TTCACGTCCT TATGACGAGG ATCGTGACGG CTTCGTCATG GGCGAAGGCG CTGGCATTGT GGTGCTTGAA GAGCTGGAAC ACGCTTTGGC GCGCGGTGCG AAGATTTATG CCGAAGTCAT CGGCTATGGC ATGTCGGGCG ATGCCTTCCA TATCACGGCC CCGACCGAGA GCGGTGAGGG CGCACAGCGC TGCATGGTGG CGGCTCTCAA GCGTGCCGGG ATCGTGCCGG ACGAGATCGA CTATATCAAT GCGCATGGCA CGTCCACCAT GGCCGATACG ATCGAGCTTG GCGCGGTGGA GCGCGTGGTG GGTGAGGCGG CTGCGAAGAT TTCGATGTCT TCCACCAAAT CATCCATCGG CCACCTGTTG GGTGCGGCAG GGGCTGCCGA AGCGGTCTTC TCCACGCTGG CCATTCGCGA CAATATCGCG CCTGCCACAC TCAATCTGGA CAATCCGGCG GCGCAGACCA GGATCGATCT CGTGCCGCAC AAGCCGCGCG AACGCAAGAT CGATGTGGCG CTGTCGAATT CGTTCGGTTT CGGTGGCACC AATGCATCGC TGGTGCTGCG CCGCTACACG GCCTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT