BrabA.00113.a
Beta-ketoacyl synthase
| CENTER ID: | BrabA.00113.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00113.a.A3.GE28065 | Created from Decode construct for feature BrabA.00113.a | 1 | 403 | ||
| BrabA.00113.a.A4.GE28066 | Created from Decode construct for feature BrabA.00113.a | 1 | 405 | ||
| BrabA.00113.a.A5.GE28067 | Created from Decode construct for feature BrabA.00113.a | 3 | 407 | ||
| BrabA.00113.a.A6.GE28068 | Created from Decode construct for feature BrabA.00113.a | 3 | 403 | ||
| BrabA.00113.a.A7.GE28069 | Created from Decode construct for feature BrabA.00113.a | 3 | 405 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00113.a.A1.PW25441 | Full length( BrabA.00113.a ) | 1 | 407 | ||
| BrabA.00113.a.A5.PW27960 | Created from Decode construct for feature BrabA.00113.a | 3 | 407 |
Structures
3LRF
DEPOSITED: 2/11/2010
DETERMINATION: XRay
CLONE: BrabA.00113.a.A1.GE25403
PROTEIN: BrabA.00113.a.A1.PW25441
3MQD
DEPOSITED: 4/28/2010
DETERMINATION: XRay
CLONE: BrabA.00113.a.A1.GE25403
PROTEIN: BrabA.00113.a.A1.PW25441
3U0E
DEPOSITED: 9/28/2011
DETERMINATION: XRay
CLONE: BrabA.00113.a.A1.GE25403
PROTEIN: BrabA.00113.a.A1.PW25441
3U0F
DEPOSITED: 9/28/2011
DETERMINATION: XRay
CLONE: BrabA.00113.a.A1.GE25403
PROTEIN: BrabA.00113.a.A1.PW25441
4JV3
DEPOSITED: 3/25/2013
DETERMINATION: XRay
CLONE: BrabA.00113.a.A1.GE25403
PROTEIN: BrabA.00113.a.A1.PW25441
Publications by SSGCID
Structural characterization of β‐ketoacyl ACP synthase I bound to platencin and fragment screening molecules at two substrate binding sites
Abendroth JA, Bryan CM, Myler PJ, Sankaran B, Forwood JK, Patterson E, Nanson J
Proteins - 2019
volume 88, issue 1, pages 47-56
PMID: 31237717; PMCID: PMC9518911
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|359391.4.peg.2262 |
| RefSeq: | YP_415493 |
| UniProt: | Q2YQQ9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRRVVVTGMG IVSSIGSNTE EVTASLREAK SGISRAEEYA ELGFRCQVHG APDIDIESLV DRRAMRFHGR GTAWNHIAMD QAIADAGLTE EEVSNERTGI IMGSGGPSTR TIVDSADITR EKGPKRVGPF AVPKAMSSTA SATLATFFKI KGINYSISSA CATSNHCIGN AYEMIQYGKQ DRMFAGGCED LDWTLSVLFD AMGAMSSKYN DTPSTASRAY DKNRDGFVIA GGAGVLVLED LETALARGAK IYGEIVGYGA TSDGYDMVAP SGEGAIRCMK MALSTVTSKI DYINPHATST PAGDAPEIEA IRQIFGAGDV CPPIAATKSL TGHSLGATGV QEAIYSLLMM QNNFICESAH IEELDPAFAD MPIVRKRIDN VQLNTVLSNS FGFGGTNATL VFQRYQG
NT Sequence
ATGCGGCGTG TGGTCGTAAC GGGTATGGGG ATCGTATCCT CAATTGGTAG CAACACCGAA GAGGTCACGG CCTCGCTGCG CGAAGCCAAA TCCGGCATCT CCCGCGCGGA AGAATATGCG GAACTGGGCT TCCGCTGCCA GGTTCACGGC GCGCCGGACA TTGATATCGA ATCACTTGTA GACCGCCGCG CCATGCGCTT CCACGGGCGG GGCACGGCAT GGAATCATAT CGCCATGGAT CAGGCGATTG CAGATGCGGG GCTGACCGAG GAGGAAGTCT CCAACGAGCG TACCGGCATC ATCATGGGTT CCGGCGGCCC GTCCACCCGC ACCATTGTCG ATTCCGCCGA TATCACCCGC GAAAAAGGCC CAAAGCGCGT CGGCCCATTC GCCGTGCCAA AAGCCATGAG CTCGACGGCT TCGGCAACAC TTGCGACCTT CTTCAAGATC AAGGGCATCA ACTATTCGAT TTCTTCGGCC TGCGCGACAT CGAATCATTG CATCGGCAAT GCCTATGAGA TGATCCAGTA TGGCAAGCAG GACCGTATGT TTGCCGGCGG CTGCGAAGAC CTCGACTGGA CACTTTCGGT TCTGTTCGAC GCCATGGGCG CCATGTCGTC CAAATATAAC GACACGCCTT CCACCGCTTC ACGCGCCTAT GACAAGAACC GCGACGGCTT CGTCATCGCT GGCGGCGCAG GCGTTCTGGT TCTTGAAGAC CTCGAAACGG CACTTGCACG CGGTGCGAAG ATCTACGGCG AAATCGTGGG CTATGGCGCA ACCTCCGACG GCTACGACAT GGTTGCACCG TCTGGCGAAG GCGCAATCCG CTGCATGAAA ATGGCGCTTT CGACCGTCAC GTCGAAGATC GATTACATCA ATCCGCACGC AACATCGACG CCTGCGGGCG ACGCCCCGGA AATCGAAGCG ATCCGCCAGA TTTTCGGCGC GGGCGACGTC TGCCCGCCAA TCGCCGCCAC CAAATCGCTC ACCGGCCATT CGCTGGGCGC AACGGGCGTG CAGGAAGCGA TCTATTCGCT TTTGATGATG CAGAACAATT TCATTTGCGA AAGCGCGCAT ATCGAAGAAC TGGACCCCGC CTTCGCGGAT ATGCCCATCG TTCGCAAGCG CATCGATAAT GTTCAGCTCA ACACCGTGCT CTCCAATTCC TTCGGGTTTG GCGGCACCAA CGCCACGCTG GTGTTTCAGC GGTATCAGGG C
Details for BrabA.00113.a.A3.GE28065
| HARVESTED ON: | 12/7/2009 |
| SEQUENCED ON: | 12/23/2009 |
| EXPECTED MW: | 45kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | No Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRRVVVTGM GIVSSIGSNT EEVTASLREA KSGISRAEEY AELGFRCQVH GAPDIDIESL VDRRAMRFHG RGTAWNHIAM DQAIADAGLT EEEVSNERTG IIMGSGGPST RTIVDSADIT REKGPKRVGP FAVPKAMSST ASATLATFFK IKGINYSISS ACATSNHCIG NAYEMIQYGK QDRMFAGGCE DLDWTLSVLF DAMGAMSSKY NDTPSTASRA YDKNRDGFVI AGGAGVLVLE DLETALARGA KIYGEIVGYG ATSDGYDMVA PSGEGAIRCM KMALSTVTSK IDYINPHATS TPAGDAPEIE AIRQIFGAGD VCPPIAATKS LTGHSLGATG VQEAIYSLLM MQNNFICESA HIEELDPAFA DMPIVRKRID NVQLNTVLSN SFGFGGTNAT LVFQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcggcgtgtg gtcgtaacgg gtatggggat cgtatcctca attggtagca acaccgaaga ggtcacggcc tcgctgcgcg aagccaaatc cggcatctcc cgcgcggaag aatatgcgga actgggcttc cgctgccagg ttcacggcgc gccggacatt gatatcgaat cacttgtaga ccgccgcgcc atgcgcttcc acgggcgggg cacggcatgg aatcatatcg ccatggatca ggcgattgca gatgcggggc tgaccgagga ggaagtctcc aacgagcgta ccggcatcat catgggttcc ggcggcccgt ccacccgcac cattgtcgat tccgccgata tcacccgcga aaaaggccca aagcgcgtcg gcccattcgc cgtgccaaaa gccatgagct cgacggcttc ggcaacactt gcgaccttct tcaagatcaa gggcatcaac tattcgattt cttcggcctg cgcgacatcg aatcattgca tcggcaatgc ctatgagatg atccagtatg gcaagcagga ccgtatgttt gccggcggct gcgaagacct cgactggaca ctttcggttc tgttcgacgc catgggcgcc atgtcgtcca aatataacga cacgccttcc accgcttcac gcgcctatga caagaaccgc gacggcttcg tcatcgctgg cggcgcaggc gttctggttc ttgaagacct cgaaacggca cttgcacgcg gtgcgaagat ctacggcgaa atcgtgggct atggcgcaac ctccgacggc tacgacatgg ttgcaccgtc tggcgaaggc gcaatccgct gcatgaaaat ggcgctttcg accgtcacgt cgaagatcga ttacatcaat ccgcacgcaa catcgacgcc tgcgggcgac gccccggaaa tcgaagcgat ccgccagatt ttcggcgcgg gcgacgtctg cccgccaatc gccgccacca aatcgctcac cggccattcg ctgggcgcaa cgggcgtgca ggaagcgatc tattcgcttt tgatgatgca gaacaatttc atttgcgaaa gcgcgcatat cgaagaactg gaccccgcct tcgcggatat gcccatcgtt cgcaagcgca tcgataatgt tcagctcaac accgtgctct ccaattcctt cgggtttggc ggcaccaacg ccacgctggt gtttcagaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BrabA.00113.a.A4.GE28066
| HARVESTED ON: | 12/7/2009 |
| SEQUENCED ON: | 2/24/2010 |
| EXPECTED MW: | 45kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 76 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRRVVVTGM GIVSSIGSNT EEVTASLREA KSGISRAEEY AELGFRCQVH GAPDIDIESL VDRRAMRFHG RGTAWNHIAM DQAIADAGLT EEEVSNERTG IIMGSGGPST RTIVDSADIT REKGPKRVGP FAVPKAMSST ASATLATFFK IKGINYSISS ACATSNHCIG NAYEMIQYGK QDRMFAGGCE DLDWTLSVLF DAMGAMSSKY NDTPSTASRA YDKNRDGFVI AGGAXVLVLE DLETALARGA KIYGEIVGYG ATSDGYDMVA PSGEXXIRCM KMALSTVTSX XDYINPHATS TPXGXX
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcggc gtgtggtcgt aacgggtatg gggatcgtat cctcaattgg tagcaacacc gaagaggtca cggcctcgct gcgcgaagcc aaatccggca tctcccgcgc ggaagaatat gcggaactgg gcttccgctg ccaggttcac ggcgcgccgg acattgatat cgaatcactt gtagaccgcc gcgccatgcg cttccacggg cggggcacgg catggaatca tatcgccatg gatcaggcga ttgcagatgc ggggctgacc gaggaggaag tctccaacga gcgtaccggc atcatcatgg gttccggcgg cccgtccacc cgcaccattg tcgattccgc cgatatcacc cgcgaaaaag gcccaaagcg cgtcggccca ttcgccgtgc caaaagccat gagctcgacg gcttcggcaa cacttgcgac cttcttcaag atcaagggca tcaactattc gatttcttcg gcctgcgcga catcgaatca ttgcatcggc aatgcctatg agatgatcca gtatggcaag caggaccgta tgtttgccgg cggctgcgaa gacctcgact ggacactttc ggttctgttc gacgccatgg gcgccatgtc gtccaaatat aacgacacgc cttccaccgc ttcacgcgcc tatgacaaga accgcgacgg cttcgtcatc gctggcggcg cangcgttct ggttcttgaa gacctcgaaa cggcacttgc acgcggtgcg aagatctacg gcgaaatcgt gggctatggc gcaacctccg acggctacga catggttgca ccgtctggcg aannnncaat ccgctgcatg aaaatggcgc tttcgaccgt cacgtcgaan ancgattaca tcaatccgca cgcaacatcg acgcctgnng gcgangnccc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRRVVVTGM GIVSSIGSNT EEVTASLREA KSGISRAEEY AELGFRCQVH GAPDIDIESL VDRRAMRFHG RGTAWNHIAM DQAIADAGLT EEEVSNERTG IIMGSGGPST RTIVDSADIT REKGPKRVGP FAVPKAMSST ASATLATFFK IKGINYSISS ACATSNHCIG NAYEMIQYGK QDRMFAGGCE DLDWTLSVLF DAMGAMSSKY NDTPSTASRA YDKNRDGFVI AGGAGVLVLE DLETALARGA KIYGEIVGYG ATSDGYDMVA PSGEGAIRCM KMALSTVTSK IDYINPHATS TPAGDAPEIE AIRQIFGAGD VCPPIAATKS LTGHSLGATG VQEAIYSLLM MQNNFICESA HIEELDPAFA DMPIVRKRID NVQLNTVLSN SFGFGGTNAT LVFQRY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcggcgtgtg gtcgtaacgg gtatggggat cgtatcctca attggtagca acaccgaaga ggtcacggcc tcgctgcgcg aagccaaatc cggcatctcc cgcgcggaag aatatgcgga actgggcttc cgctgccagg ttcacggcgc gccggacatt gatatcgaat cacttgtaga ccgccgcgcc atgcgcttcc acgggcgggg cacggcatgg aatcatatcg ccatggatca ggcgattgca gatgcggggc tgaccgagga ggaagtctcc aacgagcgta ccggcatcat catgggttcc ggcggcccgt ccacccgcac cattgtcgat tccgccgata tcacccgcga aaaaggccca aagcgcgtcg gcccattcgc cgtgccaaaa gccatgagct cgacggcttc ggcaacactt gcgaccttct tcaagatcaa gggcatcaac tattcgattt cttcggcctg cgcgacatcg aatcattgca tcggcaatgc ctatgagatg atccagtatg gcaagcagga ccgtatgttt gccggcggct gcgaagacct cgactggaca ctttcggttc tgttcgacgc catgggcgcc atgtcgtcca aatataacga cacgccttcc accgcttcac gcgcctatga caagaaccgc gacggcttcg tcatcgctgg cggcgcaggc gttctggttc ttgaagacct cgaaacggca cttgcacgcg gtgcgaagat ctacggcgaa atcgtgggct atggcgcaac ctccgacggc tacgacatgg ttgcaccgtc tggcgaaggc gcaatccgct gcatgaaaat ggcgctttcg accgtcacgt cgaagatcga ttacatcaat ccgcacgcaa catcgacgcc tgcgggcgac gccccggaaa tcgaagcgat ccgccagatt ttcggcgcgg gcgacgtctg cccgccaatc gccgccacca aatcgctcac cggccattcg ctgggcgcaa cgggcgtgca ggaagcgatc tattcgcttt tgatgatgca gaacaatttc atttgcgaaa gcgcgcatat cgaagaactg gaccccgcct tcgcggatat gcccatcgtt cgcaagcgca tcgataatgt tcagctcaac accgtgctct ccaattcctt cgggtttggc ggcaccaacg ccacgctggt gtttcagcgg tataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BrabA.00113.a.A5.GE28067
| HARVESTED ON: | 12/7/2009 |
| SEQUENCED ON: | 12/23/2009 |
| EXPECTED MW: | 45kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRVVVTGMG IVSSIGSNTE EVTASLREAK SGISRAEEYA ELGFRCQVHG APDIDIESLV DRRAMRFHGR GTAWNHIAMD QAIADAGLTE EEVSNERTGI IMGSGGPSTR TIVDSADITR EKGPKRVGPF AVPKAMSSTA SATLATFFKI KGINYSISSA CATSNHCIGN AYEMIQYGKQ DRMFAGGCED LDWTLSVLFD AMGAMSSKYN DTPSTASRAY DKNRDGFVIA GGAGVLVLED LETALARGAK IYGEIVGYGA TSDGYDMVAP SGEGAIRCMK MALSTVTSKI DYINPHATST PAGDAPEIEA IRQIFGAGDV CPPIAATKSL TGHSLGATGV QEAIYSLLMM QNNFICESAH IEELDPAFAD MPIVRKRIDN VQLNTVLSNS FGFGGTNATL VFQRYQG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtgtggtc gtaacgggta tggggatcgt atcctcaatt ggtagcaaca ccgaagaggt cacggcctcg ctgcgcgaag ccaaatccgg catctcccgc gcggaagaat atgcggaact gggcttccgc tgccaggttc acggcgcgcc ggacattgat atcgaatcac ttgtagaccg ccgcgccatg cgcttccacg ggcggggcac ggcatggaat catatcgcca tggatcaggc gattgcagat gcggggctga ccgaggagga agtctccaac gagcgtaccg gcatcatcat gggttccggc ggcccgtcca cccgcaccat tgtcgattcc gccgatatca cccgcgaaaa aggcccaaag cgcgtcggcc cattcgccgt gccaaaagcc atgagctcga cggcttcggc aacacttgcg accttcttca agatcaaggg catcaactat tcgatttctt cggcctgcgc gacatcgaat cattgcatcg gcaatgccta tgagatgatc cagtatggca agcaggaccg tatgtttgcc ggcggctgcg aagacctcga ctggacactt tcggttctgt tcgacgccat gggcgccatg tcgtccaaat ataacgacac gccttccacc gcttcacgcg cctatgacaa gaaccgcgac ggcttcgtca tcgctggcgg cgcaggcgtt ctggttcttg aagacctcga aacggcactt gcacgcggtg cgaagatcta cggcgaaatc gtgggctatg gcgcaacctc cgacggctac gacatggttg caccgtctgg cgaaggcgca atccgctgca tgaaaatggc gctttcgacc gtcacgtcga agatcgatta catcaatccg cacgcaacat cgacgcctgc gggcgacgcc ccggaaatcg aagcgatccg ccagattttc ggcgcgggcg acgtctgccc gccaatcgcc gccaccaaat cgctcaccgg ccattcgctg ggcgcaacgg gcgtgcagga agcgatctat tcgcttttga tgatgcagaa caatttcatt tgcgaaagcg cgcatatcga agaactggac cccgccttcg cggatatgcc catcgttcgc aagcgcatcg ataatgttca gctcaacacc gtgctctcca attccttcgg gtttggcggc accaacgcca cgctggtgtt tcagcggtat cagggcaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BrabA.00113.a.A6.GE28068
| HARVESTED ON: | 12/7/2009 |
| SEQUENCED ON: | 12/23/2009 |
| EXPECTED MW: | 44kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | No Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRVVVTGMG IVSSIGSNTE EVTASLREAK SGISRAEEYA ELGFRCQVHG APDIDIESLV DRRAMRFHGR GTAWNHIAMD QAIADAGLTE EEVSNERTGI IMGSGGPSTR TIVDSADITR EKGPKRVGPF AVPKAMSSTA SATLATFFKI KGINYSISSA CATSNHCIGN AYEMIQYGKQ DRMFAGGCED LDWTLSVLFD AMGAMSSKYN DTPSTASRAY DKNRDGFVIA GGAGVLVLED LETALARGAK IYGEIVGYGA TSDGYDMVAP SGEGAIRCMK MALSTVTSKI DYINPHATST PAGDAPEIEA IRQIFGAGDV CPPIAATKSL TGHSLGATGV QEAIYSLLMM QNNFICESAH IEELDPAFAD MPIVRKRIDN VQLNTVLSNS FGFGGTNATL VFQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtgtggtc gtaacgggta tggggatcgt atcctcaatt ggtagcaaca ccgaagaggt cacggcctcg ctgcgcgaag ccaaatccgg catctcccgc gcggaagaat atgcggaact gggcttccgc tgccaggttc acggcgcgcc ggacattgat atcgaatcac ttgtagaccg ccgcgccatg cgcttccacg ggcggggcac ggcatggaat catatcgcca tggatcaggc gattgcagat gcggggctga ccgaggagga agtctccaac gagcgtaccg gcatcatcat gggttccggc ggcccgtcca cccgcaccat tgtcgattcc gccgatatca cccgcgaaaa aggcccaaag cgcgtcggcc cattcgccgt gccaaaagcc atgagctcga cggcttcggc aacacttgcg accttcttca agatcaaggg catcaactat tcgatttctt cggcctgcgc gacatcgaat cattgcatcg gcaatgccta tgagatgatc cagtatggca agcaggaccg tatgtttgcc ggcggctgcg aagacctcga ctggacactt tcggttctgt tcgacgccat gggcgccatg tcgtccaaat ataacgacac gccttccacc gcttcacgcg cctatgacaa gaaccgcgac ggcttcgtca tcgctggcgg cgcaggcgtt ctggttcttg aagacctcga aacggcactt gcacgcggtg cgaagatcta cggcgaaatc gtgggctatg gcgcaacctc cgacggctac gacatggttg caccgtctgg cgaaggcgca atccgctgca tgaaaatggc gctttcgacc gtcacgtcga agatcgatta catcaatccg cacgcaacat cgacgcctgc gggcgacgcc ccggaaatcg aagcgatccg ccagattttc ggcgcgggcg acgtctgccc gccaatcgcc gccaccaaat cgctcaccgg ccattcgctg ggcgcaacgg gcgtgcagga agcgatctat tcgcttttga tgatgcagaa caatttcatt tgcgaaagcg cgcatatcga agaactggac cccgccttcg cggatatgcc catcgttcgc aagcgcatcg ataatgttca gctcaacacc gtgctctcca attccttcgg gtttggcggc accaacgcca cgctggtgtt tcagaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BrabA.00113.a.A7.GE28069
| HARVESTED ON: | 12/7/2009 |
| SEQUENCED ON: | 12/23/2009 |
| EXPECTED MW: | 45kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 72 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRVVVTGMG IVSSIGSNTE EVTASLREAK SGISRAEEYA ELGFRCQVHG APDIDIESLV DRRAMRFHGR GTAWNHIAMD QAIADAGLTE EEVSNERTGI IMGSGGPSTR TIVDSADITR EKGPKRVGPF AVPKAMSSTA SATLATFFKI KGINYSISSA CATSNHCIGN AYEMIQYGKQ DRMFAGGCED LDWTLSVLFD AMGAMSSKYN DTPSTASRAY DKNRDGFVIA GGAGVLVLED LETALARGAK IYGEIVGYGA TSDGYDMVAP SGXXAIXCMK MALSTVXS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgtg tggtcgtaac gggtatgggg atcgtatcct caattggtag caacaccgaa gaggtcacgg cctcgctgcg cgaagccaaa tccggcatct cccgcgcgga agaatatgcg gaactgggct tccgctgcca ggttcacggc gcaccggaca ttgatatcga atcacttgta gaccgccgcg ccatgcgctt ccacgggcgg ggcacggcat ggaatcatat cgccatggat caggcgattg cagatgcggg gctgaccgag gaggaagtct ccaacgagcg taccggcatc atcatgggtt ccggcggccc gtccacccgc accattgtcg attccgccga tatcacccgc gaaaaaggcc caaagcgcgt cggcccattc gccgtgccaa aagccatgag ctcgacggct tcggcaacac ttgcgacctt cttcaagatc aagggcatca actattcgat ttcttcggcc tgcgcgacat cgaatcattg catcggcaat gcctatgaga tgatccagta tggcaagcag gaccgtatgt ttgccggcgg ctgcgaagac ctcgactgga cactttcggt tctgttcgac gccatgggcg ccatgtcgtc caaatataac gacacgcctt ccaccgcttc acgcgcctat gacaagaacc gcgacggctt cgtcatcgct ggcggcgcag gcgttctggt tcttgaagac ctcgaaacgg cacttgcacg cggtgcgaag atctacggcg aaatcgtggg ctatggcgca acctccgacg gctacgacat ggttgcaccg tctggcgang nngcaatccn ctgcatgaaa atggcgcttt cgaccgtcnc gtcgaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRVVVTGMG IVSSIGSNTE EVTASLREAK SGISRAEEYA ELGFRCQVHG APDIDIESLV DRRAMRFHGR GTAWNHIAMD QAIADAGLTE EEVSNERTGI IMGSGGPSTR TIVDSADITR EKGPKRVGPF AVPKAMSSTA SATLATFFKI KGINYSISSA CATSNHCIGN AYEMIQYGKQ DRMFAGGCED LDWTLSVLFD AMGAMSSKYN DTPSTASRAY DKNRDGFVIA GGAGVLVLED LETALARGAK IYGEIVGYGA TSDGYDMVAP SGEGAIRCMK MALSTVTSKI DYINPHATST PAGDAPEIEA IRQIFGAGDV CPPIAATKSL TGHSLGATGV QEAIYSLLMM QNNFICESAH IEELDPAFAD MPIVRKRIDN VQLNTVLSNS FGFGGTNATL VFQRY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtgtggtc gtaacgggta tggggatcgt atcctcaatt ggtagcaaca ccgaagaggt cacggcctcg ctgcgcgaag ccaaatccgg catctcccgc gcggaagaat atgcggaact gggcttccgc tgccaggttc acggcgcgcc ggacattgat atcgaatcac ttgtagaccg ccgcgccatg cgcttccacg ggcggggcac ggcatggaat catatcgcca tggatcaggc gattgcagat gcggggctga ccgaggagga agtctccaac gagcgtaccg gcatcatcat gggttccggc ggcccgtcca cccgcaccat tgtcgattcc gccgatatca cccgcgaaaa aggcccaaag cgcgtcggcc cattcgccgt gccaaaagcc atgagctcga cggcttcggc aacacttgcg accttcttca agatcaaggg catcaactat tcgatttctt cggcctgcgc gacatcgaat cattgcatcg gcaatgccta tgagatgatc cagtatggca agcaggaccg tatgtttgcc ggcggctgcg aagacctcga ctggacactt tcggttctgt tcgacgccat gggcgccatg tcgtccaaat ataacgacac gccttccacc gcttcacgcg cctatgacaa gaaccgcgac ggcttcgtca tcgctggcgg cgcaggcgtt ctggttcttg aagacctcga aacggcactt gcacgcggtg cgaagatcta cggcgaaatc gtgggctatg gcgcaacctc cgacggctac gacatggttg caccgtctgg cgaaggcgca atccgctgca tgaaaatggc gctttcgacc gtcacgtcga agatcgatta catcaatccg cacgcaacat cgacgcctgc gggcgacgcc ccggaaatcg aagcgatccg ccagattttc ggcgcgggcg acgtctgccc gccaatcgcc gccaccaaat cgctcaccgg ccattcgctg ggcgcaacgg gcgtgcagga agcgatctat tcgcttttga tgatgcagaa caatttcatt tgcgaaagcg cgcatatcga agaactggac cccgccttcg cggatatgcc catcgttcgc aagcgcatcg ataatgttca gctcaacacc gtgctctcca attccttcgg gtttggcggc accaacgcca cgctggtgtt tcagcggtat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BrabA.00113.a.A1.PW25441
| PURIFICATION DATe: | 12/28/2009 |
| CONCENTRATION: | 22.94mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 29 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRRVVVTGM GIVSSIGSNT EEVTASLREA KSGISRAEEY AELGFRCQVH GAPDIDIESL VDRRAMRFHG RGTAWNHIAM DQAIADAGLT EEEVSNERTG IIMGSGGPST RTIVDSADIT REKGPKRVGP FAVPKAMSST ASATLATFFK IKGINYSISS ACATSNHCIG NAYEMIQYGK QDRMFAGGCE DLDWTLSVLF DAMGAMSSKY NDTPSTASRA YDKNRDGFVI AGGAGVLVLE DLETALARGA KIYGEIVGYG ATSDGYDMVA PSGEGAIRCM KMALSTVTSK IDYINPHATS TPAGDAPEIE AIRQIFGAGD VCPPIAATKS LTGHSLGATG VQEAIYSLLM MQNNFICESA HIEELDPAFA DMPIVRKRID NVQLNTVLSN SFGFGGTNAT LVFQRYQG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGGCGTGTG GTCGTAACGG GTATGGGGAT CGTATCCTCA ATTGGTAGCA ACACCGAAGA GGTCACGGCC TCGCTGCGCG AAGCCAAATC CGGCATCTCC CGCGCGGAAG AATATGCGGA ACTGGGCTTC CGCTGCCAGG TTCACGGCGC GCCGGACATT GATATCGAAT CACTTGTAGA CCGCCGCGCC ATGCGCTTCC ACGGGCGGGG CACGGCATGG AATCATATCG CCATGGATCA GGCGATTGCA GATGCGGGGC TGACCGAGGA GGAAGTCTCC AACGAGCGTA CCGGCATCAT CATGGGTTCC GGCGGCCCGT CCACCCGCAC CATTGTCGAT TCCGCCGATA TCACCCGCGA AAAAGGCCCA AAGCGCGTCG GCCCATTCGC CGTGCCAAAA GCCATGAGCT CGACGGCTTC GGCAACACTT GCGACCTTCT TCAAGATCAA GGGCATCAAC TATTCGATTT CTTCGGCCTG CGCGACATCG AATCATTGCA TCGGCAATGC CTATGAGATG ATCCAGTATG GCAAGCAGGA CCGTATGTTT GCCGGCGGCT GCGAAGACCT CGACTGGACA CTTTCGGTTC TGTTCGACGC CATGGGCGCC ATGTCGTCCA AATATAACGA CACGCCTTCC ACCGCTTCAC GCGCCTATGA CAAGAACCGC GACGGCTTCG TCATCGCTGG CGGCGCAGGC GTTCTGGTTC TTGAAGACCT CGAAACGGCA CTTGCACGCG GTGCGAAGAT CTACGGCGAA ATCGTGGGCT ATGGCGCAAC CTCCGACGGC TACGACATGG TTGCACCGTC TGGCGAAGGC GCAATCCGCT GCATGAAAAT GGCGCTTTCG ACCGTCACGT CGAAGATCGA TTACATCAAT CCGCACGCAA CATCGACGCC TGCGGGCGAC GCCCCGGAAA TCGAAGCGAT CCGCCAGATT TTCGGCGCGG GCGACGTCTG CCCGCCAATC GCCGCCACCA AATCGCTCAC CGGCCATTCG CTGGGCGCAA CGGGCGTGCA GGAAGCGATC TATTCGCTTT TGATGATGCA GAACAATTTC ATTTGCGAAA GCGCGCATAT CGAAGAACTG GACCCCGCCT TCGCGGATAT GCCCATCGTT CGCAAGCGCA TCGATAATGT TCAGCTCAAC ACCGTGCTCT CCAATTCCTT CGGGTTTGGC GGCACCAACG CCACGCTGGT GTTTCAGCGG TATCAGGGCT GAAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA
Details for BrabA.00113.a.A5.PW27960
| PURIFICATION DATe: | 1/25/2010 |
| CONCENTRATION: | 17.79mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRVVVTGMG IVSSIGSNTE EVTASLREAK SGISRAEEYA ELGFRCQVHG APDIDIESLV DRRAMRFHGR GTAWNHIAMD QAIADAGLTE EEVSNERTGI IMGSGGPSTR TIVDSADITR EKGPKRVGPF AVPKAMSSTA SATLATFFKI KGINYSISSA CATSNHCIGN AYEMIQYGKQ DRMFAGGCED LDWTLSVLFD AMGAMSSKYN DTPSTASRAY DKNRDGFVIA GGAGVLVLED LETALARGAK IYGEIVGYGA TSDGYDMVAP SGEGAIRCMK MALSTVTSKI DYINPHATST PAGDAPEIEA IRQIFGAGDV CPPIAATKSL TGHSLGATGV QEAIYSLLMM QNNFICESAH IEELDPAFAD MPIVRKRIDN VQLNTVLSNS FGFGGTNATL VFQRYQG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGTGTGGTC GTAACGGGTA TGGGGATCGT ATCCTCAATT GGTAGCAACA CCGAAGAGGT CACGGCCTCG CTGCGCGAAG CCAAATCCGG CATCTCCCGC GCGGAAGAAT ATGCGGAACT GGGCTTCCGC TGCCAGGTTC ACGGCGCGCC GGACATTGAT ATCGAATCAC TTGTAGACCG CCGCGCCATG CGCTTCCACG GGCGGGGCAC GGCATGGAAT CATATCGCCA TGGATCAGGC GATTGCAGAT GCGGGGCTGA CCGAGGAGGA AGTCTCCAAC GAGCGTACCG GCATCATCAT GGGTTCCGGC GGCCCGTCCA CCCGCACCAT TGTCGATTCC GCCGATATCA CCCGCGAAAA AGGCCCAAAG CGCGTCGGCC CATTCGCCGT GCCAAAAGCC ATGAGCTCGA CGGCTTCGGC AACACTTGCG ACCTTCTTCA AGATCAAGGG CATCAACTAT TCGATTTCTT CGGCCTGCGC GACATCGAAT CATTGCATCG GCAATGCCTA TGAGATGATC CAGTATGGCA AGCAGGACCG TATGTTTGCC GGCGGCTGCG AAGACCTCGA CTGGACACTT TCGGTTCTGT TCGACGCCAT GGGCGCCATG TCGTCCAAAT ATAACGACAC GCCTTCCACC GCTTCACGCG CCTATGACAA GAACCGCGAC GGCTTCGTCA TCGCTGGCGG CGCAGGCGTT CTGGTTCTTG AAGACCTCGA AACGGCACTT GCACGCGGTG CGAAGATCTA CGGCGAAATC GTGGGCTATG GCGCAACCTC CGACGGCTAC GACATGGTTG CACCGTCTGG CGAAGGCGCA ATCCGCTGCA TGAAAATGGC GCTTTCGACC GTCACGTCGA AGATCGATTA CATCAATCCG CACGCAACAT CGACGCCTGC GGGCGACGCC CCGGAAATCG AAGCGATCCG CCAGATTTTC GGCGCGGGCG ACGTCTGCCC GCCAATCGCC GCCACCAAAT CGCTCACCGG CCATTCGCTG GGCGCAACGG GCGTGCAGGA AGCGATCTAT TCGCTTTTGA TGATGCAGAA CAATTTCATT TGCGAAAGCG CGCATATCGA AGAACTGGAC CCCGCCTTCG CGGATATGCC CATCGTTCGC AAGCGCATCG ATAATGTTCA GCTCAACACC GTGCTCTCCA ATTCCTTCGG GTTTGGCGGC ACCAACGCCA CGCTGGTGTT TCAGCGGTAT CAGGGCAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA